qpcrs Search Results


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  • 99
    Qiagen rt2 qpcr primer assay
    Induction of RA metabolic enzymes in decidualizing cells. (a) Expression of DHRS3 , RDH12 , RBP4 and <t>CYP26A1</t> transcripts in undifferentiated or cells decidualized with 8-bromo-cAMP, P4, and E in the presence or absence of 10 −8 M atRA or 10 −8 M atRald for 8 days (n = 9–11). The results show fold-change (mean ± SEM) in mRNA levels relative to expression levels in vehicle-treated undifferentiated HESCs ( dotted lines ). (b) Representative Western blot analysis of culture treated in parallel. β-actin served as a loading control. * P
    Rt2 Qpcr Primer Assay, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Jena Bioscience qpcr greenmaster
    Induction of RA metabolic enzymes in decidualizing cells. (a) Expression of DHRS3 , RDH12 , RBP4 and <t>CYP26A1</t> transcripts in undifferentiated or cells decidualized with 8-bromo-cAMP, P4, and E in the presence or absence of 10 −8 M atRA or 10 −8 M atRald for 8 days (n = 9–11). The results show fold-change (mean ± SEM) in mRNA levels relative to expression levels in vehicle-treated undifferentiated HESCs ( dotted lines ). (b) Representative Western blot analysis of culture treated in parallel. β-actin served as a loading control. * P
    Qpcr Greenmaster, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 99/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega gotaq qpcr and rt qpcr systems
    Performance comparison of two different commercial kits for WSSV detection by <t>qPCR.</t> The solid line represents iQ SYBR® Green supermix (Biorad) amplification, whereas the dashed line represents amplification using <t>GoTaq</t> qPCR Master mix (Promega). No differences were detected.
    Gotaq Qpcr And Rt Qpcr Systems, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Promega qpcr master mix
    Performance comparison of two different commercial kits for WSSV detection by <t>qPCR.</t> The solid line represents iQ SYBR® Green supermix (Biorad) amplification, whereas the dashed line represents amplification using <t>GoTaq</t> qPCR Master mix (Promega). No differences were detected.
    Qpcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 97/100, based on 286 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Horizon Discovery qpcrs
    Performance comparison of two different commercial kits for WSSV detection by <t>qPCR.</t> The solid line represents iQ SYBR® Green supermix (Biorad) amplification, whereas the dashed line represents amplification using <t>GoTaq</t> qPCR Master mix (Promega). No differences were detected.
    Qpcrs, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad qpcrs
    Performance comparison of two different commercial kits for WSSV detection by <t>qPCR.</t> The solid line represents iQ SYBR® Green supermix (Biorad) amplification, whereas the dashed line represents amplification using <t>GoTaq</t> qPCR Master mix (Promega). No differences were detected.
    Qpcrs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1780 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Genecopoeia qpcrs
    Performance comparison of two different commercial kits for WSSV detection by <t>qPCR.</t> The solid line represents iQ SYBR® Green supermix (Biorad) amplification, whereas the dashed line represents amplification using <t>GoTaq</t> qPCR Master mix (Promega). No differences were detected.
    Qpcrs, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher qpcrs
    Performance comparison of two different commercial kits for WSSV detection by <t>qPCR.</t> The solid line represents iQ SYBR® Green supermix (Biorad) amplification, whereas the dashed line represents amplification using <t>GoTaq</t> qPCR Master mix (Promega). No differences were detected.
    Qpcrs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3817 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher qpcr
    Performance comparison of two different commercial kits for WSSV detection by <t>qPCR.</t> The solid line represents iQ SYBR® Green supermix (Biorad) amplification, whereas the dashed line represents amplification using <t>GoTaq</t> qPCR Master mix (Promega). No differences were detected.
    Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 17019 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega gotaq probe qpcr and rt qpcr systems
    Performance comparison of two different commercial kits for WSSV detection by <t>qPCR.</t> The solid line represents iQ SYBR® Green supermix (Biorad) amplification, whereas the dashed line represents amplification using <t>GoTaq</t> qPCR Master mix (Promega). No differences were detected.
    Gotaq Probe Qpcr And Rt Qpcr Systems, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Jena Bioscience qpcr probesmaster
    Performance comparison of two different commercial kits for WSSV detection by <t>qPCR.</t> The solid line represents iQ SYBR® Green supermix (Biorad) amplification, whereas the dashed line represents amplification using <t>GoTaq</t> qPCR Master mix (Promega). No differences were detected.
    Qpcr Probesmaster, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Promega gotap qpcr master mix
    Performance comparison of two different commercial kits for WSSV detection by <t>qPCR.</t> The solid line represents iQ SYBR® Green supermix (Biorad) amplification, whereas the dashed line represents amplification using <t>GoTaq</t> qPCR Master mix (Promega). No differences were detected.
    Gotap Qpcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 96/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher qpcr supermix
    ERβ-dependent changes in gene expression are regulated in opposite directions in ECs derived from different vascular beds. qRT–PCR validation of TAC results was carried out using Roche Universal ProbeLibrary (Roche Applied Science) with Express <t>qPCR</t> <t>Supermix</t> (Invitrogen) on HEECs, UtMVECs and HUVECs, treated with the vehicle control (DMSO), DPN (10 −8 M) or DPN plus the anti-estrogen Fulvestrant (ICI, 10 −7 M). Values represent the mean ± SEM, n = 5. Results are normalized to the vehicle control where one replicate was given the arbitrary value of 1. RQ, relative quantification (* P
    Qpcr Supermix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Promega sybr green qpcr master mix
    ERβ-dependent changes in gene expression are regulated in opposite directions in ECs derived from different vascular beds. qRT–PCR validation of TAC results was carried out using Roche Universal ProbeLibrary (Roche Applied Science) with Express <t>qPCR</t> <t>Supermix</t> (Invitrogen) on HEECs, UtMVECs and HUVECs, treated with the vehicle control (DMSO), DPN (10 −8 M) or DPN plus the anti-estrogen Fulvestrant (ICI, 10 −7 M). Values represent the mean ± SEM, n = 5. Results are normalized to the vehicle control where one replicate was given the arbitrary value of 1. RQ, relative quantification (* P
    Sybr Green Qpcr Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 97/100, based on 143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega qpcr kit gotag qpcr
    ERβ-dependent changes in gene expression are regulated in opposite directions in ECs derived from different vascular beds. qRT–PCR validation of TAC results was carried out using Roche Universal ProbeLibrary (Roche Applied Science) with Express <t>qPCR</t> <t>Supermix</t> (Invitrogen) on HEECs, UtMVECs and HUVECs, treated with the vehicle control (DMSO), DPN (10 −8 M) or DPN plus the anti-estrogen Fulvestrant (ICI, 10 −7 M). Values represent the mean ± SEM, n = 5. Results are normalized to the vehicle control where one replicate was given the arbitrary value of 1. RQ, relative quantification (* P
    Qpcr Kit Gotag Qpcr, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore luminoct qpcr readymix
    ERβ-dependent changes in gene expression are regulated in opposite directions in ECs derived from different vascular beds. qRT–PCR validation of TAC results was carried out using Roche Universal ProbeLibrary (Roche Applied Science) with Express <t>qPCR</t> <t>Supermix</t> (Invitrogen) on HEECs, UtMVECs and HUVECs, treated with the vehicle control (DMSO), DPN (10 −8 M) or DPN plus the anti-estrogen Fulvestrant (ICI, 10 −7 M). Values represent the mean ± SEM, n = 5. Results are normalized to the vehicle control where one replicate was given the arbitrary value of 1. RQ, relative quantification (* P
    Luminoct Qpcr Readymix, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Zymo Research questtaq qpcr premix
    ERβ-dependent changes in gene expression are regulated in opposite directions in ECs derived from different vascular beds. qRT–PCR validation of TAC results was carried out using Roche Universal ProbeLibrary (Roche Applied Science) with Express <t>qPCR</t> <t>Supermix</t> (Invitrogen) on HEECs, UtMVECs and HUVECs, treated with the vehicle control (DMSO), DPN (10 −8 M) or DPN plus the anti-estrogen Fulvestrant (ICI, 10 −7 M). Values represent the mean ± SEM, n = 5. Results are normalized to the vehicle control where one replicate was given the arbitrary value of 1. RQ, relative quantification (* P
    Questtaq Qpcr Premix, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Zymo Research zymotaq qpcr premix
    ERβ-dependent changes in gene expression are regulated in opposite directions in ECs derived from different vascular beds. qRT–PCR validation of TAC results was carried out using Roche Universal ProbeLibrary (Roche Applied Science) with Express <t>qPCR</t> <t>Supermix</t> (Invitrogen) on HEECs, UtMVECs and HUVECs, treated with the vehicle control (DMSO), DPN (10 −8 M) or DPN plus the anti-estrogen Fulvestrant (ICI, 10 −7 M). Values represent the mean ± SEM, n = 5. Results are normalized to the vehicle control where one replicate was given the arbitrary value of 1. RQ, relative quantification (* P
    Zymotaq Qpcr Premix, supplied by Zymo Research, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore qpcr probes
    Determination of intracellular <t>Ca2+</t> levels in <t>NPCs</t>
    Qpcr Probes, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    DaAn Gene taqman qpcr taqman qpcr kits
    Determination of intracellular <t>Ca2+</t> levels in <t>NPCs</t>
    Taqman Qpcr Taqman Qpcr Kits, supplied by DaAn Gene, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher qpcr supermix universal
    Determination of intracellular <t>Ca2+</t> levels in <t>NPCs</t>
    Qpcr Supermix Universal, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher absolute qpcr seals
    Quantitative <t>PCR.</t>
    Absolute Qpcr Seals, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher absolute qpcr
    Quantitative <t>PCR.</t>
    Absolute Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher dynamo probe qpcr
    Replication of vaccine candidates in suckling mouse brain assayed by quantitative <t>PCR.</t> Five-day-old Swiss Webster mice were inoculated i.c. with 1x10 5 FFU of the indicated virus. On days 1, 3, 5, 7, 9, 14, and 21, three mice per group were sacrificed
    Dynamo Probe Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore kicqstart probe qpcr readymix
    Replication of vaccine candidates in suckling mouse brain assayed by quantitative <t>PCR.</t> Five-day-old Swiss Webster mice were inoculated i.c. with 1x10 5 FFU of the indicated virus. On days 1, 3, 5, 7, 9, 14, and 21, three mice per group were sacrificed
    Kicqstart Probe Qpcr Readymix, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Endress+Hauser innumix qpcr mastermix sygreen qpcr kit
    Replication of vaccine candidates in suckling mouse brain assayed by quantitative <t>PCR.</t> Five-day-old Swiss Webster mice were inoculated i.c. with 1x10 5 FFU of the indicated virus. On days 1, 3, 5, 7, 9, 14, and 21, three mice per group were sacrificed
    Innumix Qpcr Mastermix Sygreen Qpcr Kit, supplied by Endress+Hauser, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher absolute qpcr rox
    Replication of vaccine candidates in suckling mouse brain assayed by quantitative <t>PCR.</t> Five-day-old Swiss Webster mice were inoculated i.c. with 1x10 5 FFU of the indicated virus. On days 1, 3, 5, 7, 9, 14, and 21, three mice per group were sacrificed
    Absolute Qpcr Rox, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa bacpak qpcr titration kit
    Replication of vaccine candidates in suckling mouse brain assayed by quantitative <t>PCR.</t> Five-day-old Swiss Webster mice were inoculated i.c. with 1x10 5 FFU of the indicated virus. On days 1, 3, 5, 7, 9, 14, and 21, three mice per group were sacrificed
    Bacpak Qpcr Titration Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Induction of RA metabolic enzymes in decidualizing cells. (a) Expression of DHRS3 , RDH12 , RBP4 and CYP26A1 transcripts in undifferentiated or cells decidualized with 8-bromo-cAMP, P4, and E in the presence or absence of 10 −8 M atRA or 10 −8 M atRald for 8 days (n = 9–11). The results show fold-change (mean ± SEM) in mRNA levels relative to expression levels in vehicle-treated undifferentiated HESCs ( dotted lines ). (b) Representative Western blot analysis of culture treated in parallel. β-actin served as a loading control. * P

    Journal: PLoS ONE

    Article Title: Reprogramming of the retinoic acid pathway in decidualizing human endometrial stromal cells

    doi: 10.1371/journal.pone.0173035

    Figure Lengend Snippet: Induction of RA metabolic enzymes in decidualizing cells. (a) Expression of DHRS3 , RDH12 , RBP4 and CYP26A1 transcripts in undifferentiated or cells decidualized with 8-bromo-cAMP, P4, and E in the presence or absence of 10 −8 M atRA or 10 −8 M atRald for 8 days (n = 9–11). The results show fold-change (mean ± SEM) in mRNA levels relative to expression levels in vehicle-treated undifferentiated HESCs ( dotted lines ). (b) Representative Western blot analysis of culture treated in parallel. β-actin served as a loading control. * P

    Article Snippet: We used commercially available primers for FABP5 (330001 PPH02412E, QIAGEN, Mayland, USA) and Cyp26a1 (330001 PPH01229A, QIAGEN, Mayland, USA).

    Techniques: Expressing, Western Blot

    Schematic overview of the RA pathway. Retinol, hydrolyzed from retinyl ester, is converted to retinaldehyde (Rald) and generates retinoic acid (RA) following two-step oxidation. Intracellular RA binds to CRABP2 and activates heterodimer, RAR and RXR, leading to apoptotic machinery. By contrast, RA-dependent activation of nuclear receptor, PPAR β/δ is mediated by FABP5.

    Journal: PLoS ONE

    Article Title: Reprogramming of the retinoic acid pathway in decidualizing human endometrial stromal cells

    doi: 10.1371/journal.pone.0173035

    Figure Lengend Snippet: Schematic overview of the RA pathway. Retinol, hydrolyzed from retinyl ester, is converted to retinaldehyde (Rald) and generates retinoic acid (RA) following two-step oxidation. Intracellular RA binds to CRABP2 and activates heterodimer, RAR and RXR, leading to apoptotic machinery. By contrast, RA-dependent activation of nuclear receptor, PPAR β/δ is mediated by FABP5.

    Article Snippet: We used commercially available primers for FABP5 (330001 PPH02412E, QIAGEN, Mayland, USA) and Cyp26a1 (330001 PPH01229A, QIAGEN, Mayland, USA).

    Techniques: Activation Assay

    Expression of cellular retinoic acid binding proteins in decidualizing HESCs. (a) CRABP2 and FABP5 transcript levels in undifferentiated or decidualized cells treated with 8-bromo-cAMP, P4, and E in combination with or without 10 −8 M atRA or 10 −8 M atRald for 8 days. The results show fold-change (mean ± SEM) relative to vehicle control ( dotted lines ). CRABP2 mRNA was measured in 8 primary HESC cultures and FABP5 mRNA in 9 cultures. (b) Representative Western blot analysis of retinoic acid binding proteins in whole cell lysates from undifferentiated or cells decidualized in the absence or presence of 10 −8 M atRA or 10 −8 M atRald. β-actin served as a loading control. E, cortisone; RA, retinoic acid; Rald, retinaldehyde. * indicates P

    Journal: PLoS ONE

    Article Title: Reprogramming of the retinoic acid pathway in decidualizing human endometrial stromal cells

    doi: 10.1371/journal.pone.0173035

    Figure Lengend Snippet: Expression of cellular retinoic acid binding proteins in decidualizing HESCs. (a) CRABP2 and FABP5 transcript levels in undifferentiated or decidualized cells treated with 8-bromo-cAMP, P4, and E in combination with or without 10 −8 M atRA or 10 −8 M atRald for 8 days. The results show fold-change (mean ± SEM) relative to vehicle control ( dotted lines ). CRABP2 mRNA was measured in 8 primary HESC cultures and FABP5 mRNA in 9 cultures. (b) Representative Western blot analysis of retinoic acid binding proteins in whole cell lysates from undifferentiated or cells decidualized in the absence or presence of 10 −8 M atRA or 10 −8 M atRald. β-actin served as a loading control. E, cortisone; RA, retinoic acid; Rald, retinaldehyde. * indicates P

    Article Snippet: We used commercially available primers for FABP5 (330001 PPH02412E, QIAGEN, Mayland, USA) and Cyp26a1 (330001 PPH01229A, QIAGEN, Mayland, USA).

    Techniques: Expressing, Binding Assay, Western Blot

    Performance comparison of two different commercial kits for WSSV detection by qPCR. The solid line represents iQ SYBR® Green supermix (Biorad) amplification, whereas the dashed line represents amplification using GoTaq qPCR Master mix (Promega). No differences were detected.

    Journal: Virology Journal

    Article Title: Development and validation of a quantitative real-time polymerase chain assay for universal detection of the White Spot Syndrome Virus in marine crustaceans

    doi: 10.1186/1743-422X-10-186

    Figure Lengend Snippet: Performance comparison of two different commercial kits for WSSV detection by qPCR. The solid line represents iQ SYBR® Green supermix (Biorad) amplification, whereas the dashed line represents amplification using GoTaq qPCR Master mix (Promega). No differences were detected.

    Article Snippet: In addition, in order to compare the assay precision, qPCR was also conducted with the dilution DNA samples containing 1.24 × 108 and 1.24 × 102 copies/μL in triplicate using a different detection chemistry kit, the GoTaq® qPCR Master mix (Promega).

    Techniques: Real-time Polymerase Chain Reaction, SYBR Green Assay, Amplification

    ERβ-dependent changes in gene expression are regulated in opposite directions in ECs derived from different vascular beds. qRT–PCR validation of TAC results was carried out using Roche Universal ProbeLibrary (Roche Applied Science) with Express qPCR Supermix (Invitrogen) on HEECs, UtMVECs and HUVECs, treated with the vehicle control (DMSO), DPN (10 −8 M) or DPN plus the anti-estrogen Fulvestrant (ICI, 10 −7 M). Values represent the mean ± SEM, n = 5. Results are normalized to the vehicle control where one replicate was given the arbitrary value of 1. RQ, relative quantification (* P

    Journal: Human Reproduction (Oxford, England)

    Article Title: ER?-dependent effects on uterine endothelial cells are cell specific and mediated via Sp1

    doi: 10.1093/humrep/det235

    Figure Lengend Snippet: ERβ-dependent changes in gene expression are regulated in opposite directions in ECs derived from different vascular beds. qRT–PCR validation of TAC results was carried out using Roche Universal ProbeLibrary (Roche Applied Science) with Express qPCR Supermix (Invitrogen) on HEECs, UtMVECs and HUVECs, treated with the vehicle control (DMSO), DPN (10 −8 M) or DPN plus the anti-estrogen Fulvestrant (ICI, 10 −7 M). Values represent the mean ± SEM, n = 5. Results are normalized to the vehicle control where one replicate was given the arbitrary value of 1. RQ, relative quantification (* P

    Article Snippet: Additional real-time PCR reactions were performed using the Roche Universal ProbeLibrary (Roche Applied Science, West Sussex, UK) and Express qPCR Supermix (Invitrogen). qPCR was performed on a 7900 Fast Real-Time PCR machine with 18S as the endogenous control.

    Techniques: Expressing, Derivative Assay, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    Determination of intracellular Ca2+ levels in NPCs

    Journal: Experimental Neurobiology

    Article Title: Restorative Mechanism of Neural Progenitor Cells Overexpressing Arginine Decarboxylase Genes Following Ischemic Injury

    doi: 10.5607/en.2019.28.1.85

    Figure Lengend Snippet: Determination of intracellular Ca2+ levels in NPCs

    Article Snippet: Determination of intracellular Ca2+ levels in NPCs We chemically dissociated the NPCs into single cells and attached them to a glass bottom dish (SPL, Republic of Korea) coated with poly-L-ornithine (P4957, Sigma) for 1 h. NPCs were loaded with the Ca2+ -sensitive green fluorescence indicator Fluo-4 AM (4 µM, F14217; Molecular Probes®) diluted in Tyrode's buffer (2Ca5K solution (319 mOsm) containing 2 mM CaCl2 , 1 mM MgCl2 , 140 mM NaCl, 5 mM KCl, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (pH 7.4, Sigma), 10 mM glucose, and 0.5 M ethylenediaminetetraacetic acid (EDTA) at 37℃ for 30 min. We prepared zero-calcium solutions by replacing CaCl2 with equimolar MgCl2 and 0.5 mM EDTA [ ].

    Techniques:

    SF2-GFP redistributes to the vicinity of the GC component B23 in response to UV irradiation. (A) A diagram of the nucleolus with the fibrillar center (FC) in the middle surrounded by a dense fibrillar component (DFC) and a granular component (GC) in an

    Journal: Nucleus

    Article Title: SR and SR-related proteins redistribute to segregated fibrillar components of nucleoli in a response to DNA damage

    doi: 10.4161/nucl.1.4.12683

    Figure Lengend Snippet: SF2-GFP redistributes to the vicinity of the GC component B23 in response to UV irradiation. (A) A diagram of the nucleolus with the fibrillar center (FC) in the middle surrounded by a dense fibrillar component (DFC) and a granular component (GC) in an

    Article Snippet: The secondary antibodies used were: Alexa Fluor 546-conjugated donkey anti-goat IgG (H + L) (Molecular Probes, Eugene, OR, USA) for U2AF35 , hSlu7, hnRNP A1 and B23; Cy3-conjugated donkey anti-mouse IgG (Chemicon, Temecula, CA, USA) for SC35, U1-70K, p32, SMN, 8WG16, FLAG and BrdU; Fluorolink Cy3-conjugated goat anti-rabbit IgG (H + L) (GE Healthcare, Tokyo, Japan) for U5-116K and HSF1; Alexa Fluor 488-conjugated rabbit anti-goat IgG (Molecular Probes) for B23; and Alexa Fluor 488-conjugated donkey anti-mouse IgG (Molecular Probes) for SF2/ASF, CUG-BP1, U2AF65 , TAP, FLAG, T7 and BrdU.

    Techniques: Irradiation

    Quantitative PCR.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Identification of Immune Parameters To Differentiate Disease States among Sheep Infected with Mycobacterium avium subsp. paratuberculosis ▿

    doi: 10.1128/CVI.00359-09

    Figure Lengend Snippet: Quantitative PCR.

    Article Snippet: TaqMan assays were designed to span intron sequences (Table ), as predicted by alignment of the ovine sequences against the complete bovine genome database. qPCRs were carried out in MicroAmp Fast optical 96-well reaction plates (Applied Biosystems) sealed with ABsolute qPCR seals (ABgene) by combining the TaqMan universal PCR master mix (Applied Biosystems) with the primer at 900 nM and the probe at 100 nM.

    Techniques: Real-time Polymerase Chain Reaction

    Replication of vaccine candidates in suckling mouse brain assayed by quantitative PCR. Five-day-old Swiss Webster mice were inoculated i.c. with 1x10 5 FFU of the indicated virus. On days 1, 3, 5, 7, 9, 14, and 21, three mice per group were sacrificed

    Journal: Virology

    Article Title: A replication-deficient rabies virus vaccine expressing Ebola virus glycoprotein is highly attenuated for neurovirulence

    doi: 10.1016/j.virol.2012.07.020

    Figure Lengend Snippet: Replication of vaccine candidates in suckling mouse brain assayed by quantitative PCR. Five-day-old Swiss Webster mice were inoculated i.c. with 1x10 5 FFU of the indicated virus. On days 1, 3, 5, 7, 9, 14, and 21, three mice per group were sacrificed

    Article Snippet: Real-time PCR was performed on an ABI 7900HT Fast machine using a Dynamo Probe qPCR kit (Thermo Scientific) ( ).

    Techniques: Real-time Polymerase Chain Reaction, Mouse Assay