qpcr reactions Search Results


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  • 97
    Roche quantitative polymerase chain reaction qpcr
    Quantitative PCR results of the 3 confirmed <t>CNV</t> differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female
    Quantitative Polymerase Chain Reaction Qpcr, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative polymerase chain reaction qpcr
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Quantitative Polymerase Chain Reaction Qpcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad quantitative polymerase chain reaction qpcr quantitative pcr
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Quantitative Polymerase Chain Reaction Qpcr Quantitative Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative polymerase chain reaction qpcr
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Quantitative Polymerase Chain Reaction Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1508 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche quantitative pcr analysis a lightcyler 96 quantitative polymerase chain reaction qpcr system
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Quantitative Pcr Analysis A Lightcyler 96 Quantitative Polymerase Chain Reaction Qpcr System, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa quantitative polymerase chain reaction pcr kits
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Quantitative Polymerase Chain Reaction Pcr Kits, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG fluorescence quantitative polymerase chain reaction pcr
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Fluorescence Quantitative Polymerase Chain Reaction Pcr, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG fluorescent quantitative polymerase chain reaction pcr
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Fluorescent Quantitative Polymerase Chain Reaction Pcr, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative polymerase chain reaction qpcr kit
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Quantitative Polymerase Chain Reaction Qpcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore quantitative polymerase chain reaction qpcr primers
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Quantitative Polymerase Chain Reaction Qpcr Primers, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative polymerase chain reaction qpcr kits
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Quantitative Polymerase Chain Reaction Qpcr Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler quantitative polymerase chain reaction pcr
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Lightcycler Quantitative Polymerase Chain Reaction Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co quantitative polymerase chain reaction qpcr kit
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Quantitative Polymerase Chain Reaction Qpcr Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kapa Biosystems quantitative polymerase chain reaction qpcr reagent
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Quantitative Polymerase Chain Reaction Qpcr Reagent, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SuperArray Bioscience Corporation superarray quantitative polymerase chain reaction pcr assay
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Superarray Quantitative Polymerase Chain Reaction Pcr Assay, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene quantitative polymerase chain reaction qpcr machine
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Quantitative Polymerase Chain Reaction Qpcr Machine, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7900ht quantitative polymerase chain reaction pcr instrument
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    7900ht Quantitative Polymerase Chain Reaction Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fluorescence quantitative polymerase chain reaction pcr kit
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Fluorescence Quantitative Polymerase Chain Reaction Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega fluorescent quantitative polymerase chain reaction pcr kit
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Fluorescent Quantitative Polymerase Chain Reaction Pcr Kit, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche quantitative polymerase chain reaction pcr reactions
    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) <t>qRT-PCR</t> analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P
    Quantitative Polymerase Chain Reaction Pcr Reactions, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative real time polymerase chain reaction pcr quantitative real time polymerase chain reaction
    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) <t>qRT-PCR</t> analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P
    Quantitative Real Time Polymerase Chain Reaction Pcr Quantitative Real Time Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7900ht quantitative polymerase chain reaction qpcr machine
    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) <t>qRT-PCR</t> analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P
    7900ht Quantitative Polymerase Chain Reaction Qpcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore quantitative polymerase chain reaction qpcr trizol reagent
    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) <t>qRT-PCR</t> analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P
    Quantitative Polymerase Chain Reaction Qpcr Trizol Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher transcriptase quantitative polymerase chain reaction qpcr
    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) <t>qRT-PCR</t> analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P
    Transcriptase Quantitative Polymerase Chain Reaction Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mx3005p quantitative polymerase chain reaction qpcr system
    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) <t>qRT-PCR</t> analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P
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    Thermo Fisher fluorescence quantitative polymerase chain reaction pcr instrument
    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) <t>qRT-PCR</t> analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P
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    Bio-Rad fluorescence quantitative polymerase chain reaction pcr instrument
    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) <t>qRT-PCR</t> analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P
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    Shanghai Kehua fluorescence quantitative polymerase chain reaction fq pcr
    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) <t>qRT-PCR</t> analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P
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    Bio-Rad myiq quantitative polymerase chain reaction pcr machine
    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) <t>qRT-PCR</t> analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P
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    Agilent technologies quantitative polymerase chain reaction pcr reactions
    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) <t>qRT-PCR</t> analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P
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    Stratagene mx3005p quantitative polymerase chain reaction qpcr system
    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) <t>qRT-PCR</t> analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P
    Mx3005p Quantitative Polymerase Chain Reaction Qpcr System, supplied by Stratagene, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative polymerase chain reaction qpcr trizol reagent
    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) <t>qRT-PCR</t> analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P
    Quantitative Polymerase Chain Reaction Qpcr Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Quantitative PCR results of the 3 confirmed CNV differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female

    Journal: Molecular Syndromology

    Article Title: Differences in Copy Number Variation between Discordant Monozygotic Twins as a Model for Exploring Chromosomal Mosaicism in Congenital Heart Defects

    doi: 10.1159/000335284

    Figure Lengend Snippet: Quantitative PCR results of the 3 confirmed CNV differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female

    Article Snippet: To confirm the presence of CNV differences, quantitative polymerase chain reaction (qPCR) was performed on DNA from both twin members and 3 unrelated controls (2 males and 1 female) using the LightCycler® 480 Real-Time PCR system (Roche Applied Science, Belgium, ), following the manufacturer's instructions.

    Techniques: Real-time Polymerase Chain Reaction

    Transcript abundance of mitochondrial mRNAs in Arabidopsis wild-type and mterf22 plants grown at 28°C. The accumulation of various mtRNAs in wild-type and mterf22 plants grown at 28°C was analyzed by quantitative reverse transcription PCR (RT-qPCR). Total RNA (depleted from DNA) was extracted from 3-week-old wild-type (Col-0) and mutant plants, reverse-transcribed, and the relative steady-state levels of different organellar transcripts were evaluated by qPCR with specific oligonucleotides, after normalization to the actin2 ). The histogram shows the relative mRNAs levels in mutant lines versus those of wild-type plants (i.e., log2 ratios of mutant to wild-type mtRNAs). Transcripts analyzed in these assays include the NADH dehydrogenase (i.e., complex I) subunits nad1 exons a-b, b-c, c-d, d-e, nad2 exons a-b, b-c, c-d, d-e, nad3 , nad4 exons a-b, b-c, c-d, nad4L , nad5 exons a-b, b-c, c-d, d-e, the intronless nad6 subunit, nad7 exons a-b, b-c, c-d, d-e, and nad9 , the complex III cob subunit, cytochrome oxidase (complex IV) cox1 , cox2 exons a-b and cox3 subunits, the ATP synthase (i.e., complex V) subunits atp1 , atp4 , atp6 , atp8 and atp9 , genes encoding the cytochrome c maturation ( ccm ) factors, ccmB , ccmC , ccmFn1 , ccmFn2 and ccmFc exons a-b, the ribosomal subunits rpl2 exons a-b, rps3 exons a-b, rps4 , rps7 , rps12 , rpl16 , rrn26 , and the mttB gene. The values are means of five biological replicates (error bars indicate one standard deviation).

    Journal: PLoS ONE

    Article Title: Control of organelle gene expression by the mitochondrial transcription termination factor mTERF22 in Arabidopsis thaliana plants

    doi: 10.1371/journal.pone.0201631

    Figure Lengend Snippet: Transcript abundance of mitochondrial mRNAs in Arabidopsis wild-type and mterf22 plants grown at 28°C. The accumulation of various mtRNAs in wild-type and mterf22 plants grown at 28°C was analyzed by quantitative reverse transcription PCR (RT-qPCR). Total RNA (depleted from DNA) was extracted from 3-week-old wild-type (Col-0) and mutant plants, reverse-transcribed, and the relative steady-state levels of different organellar transcripts were evaluated by qPCR with specific oligonucleotides, after normalization to the actin2 ). The histogram shows the relative mRNAs levels in mutant lines versus those of wild-type plants (i.e., log2 ratios of mutant to wild-type mtRNAs). Transcripts analyzed in these assays include the NADH dehydrogenase (i.e., complex I) subunits nad1 exons a-b, b-c, c-d, d-e, nad2 exons a-b, b-c, c-d, d-e, nad3 , nad4 exons a-b, b-c, c-d, nad4L , nad5 exons a-b, b-c, c-d, d-e, the intronless nad6 subunit, nad7 exons a-b, b-c, c-d, d-e, and nad9 , the complex III cob subunit, cytochrome oxidase (complex IV) cox1 , cox2 exons a-b and cox3 subunits, the ATP synthase (i.e., complex V) subunits atp1 , atp4 , atp6 , atp8 and atp9 , genes encoding the cytochrome c maturation ( ccm ) factors, ccmB , ccmC , ccmFn1 , ccmFn2 and ccmFc exons a-b, the ribosomal subunits rpl2 exons a-b, rps3 exons a-b, rps4 , rps7 , rps12 , rpl16 , rrn26 , and the mttB gene. The values are means of five biological replicates (error bars indicate one standard deviation).

    Article Snippet: Quantitative PCR (qPCR) reactions were run on a 384-well LightCycler 480® Instrument II (Roche, Product no. 05015243001), using 2.5 μl of LightCycler 480 SYBR Green I Master mix and 2.5 μM forward and reverse primers in a final volume of 5 μl.

    Techniques: Polymerase Chain Reaction, Quantitative RT-PCR, Mutagenesis, Real-time Polymerase Chain Reaction, Standard Deviation

    ( A ) qPCR data of Bacterial and Archaeal 16S copy numbers (per g biomass) on the right y-axis from biomass samples (x-axis) throughout the trial corresponding to the ratio of bacteria to archaea (expressed as a percentage) on the left y-axis. ( B ) qPCR data of Bacterial and Archaeal 16S rRNA transcripts copy numbers (per g biomass) on the right y-axis from biomass samples (x-axis) throughout the trial corresponding to the ratio of bacteria to archaea (expressed as a percentage) on the left y-axis.

    Journal: FEMS Microbiology Ecology

    Article Title: Cold adaptation and replicable microbial community development during long-term low-temperature anaerobic digestion treatment of synthetic sewage

    doi: 10.1093/femsec/fiy095

    Figure Lengend Snippet: ( A ) qPCR data of Bacterial and Archaeal 16S copy numbers (per g biomass) on the right y-axis from biomass samples (x-axis) throughout the trial corresponding to the ratio of bacteria to archaea (expressed as a percentage) on the left y-axis. ( B ) qPCR data of Bacterial and Archaeal 16S rRNA transcripts copy numbers (per g biomass) on the right y-axis from biomass samples (x-axis) throughout the trial corresponding to the ratio of bacteria to archaea (expressed as a percentage) on the left y-axis.

    Article Snippet: Quantitative-polymerase chain reaction Quantitative polymerase chain reaction (qPCR) was carried out for Archaeal and Bacterial domains using DNA and cDNA generated from granular biomass sampled from R1 and R2 and the fixed-film filter as described above. qPCR was performed using a LightCycler 480 (Roche, Manheim, Germany).

    Techniques: Real-time Polymerase Chain Reaction

    miR-296 mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after alkali exposures

    doi: 10.3892/etm.2017.5408

    Figure Lengend Snippet: miR-296 mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P

    Article Snippet: Total RNA sample preparation and quantitative polymerase chain reaction (qPCR) To quantify miR-296 mRNA expression levels, frozen mice corneal tissues were dissolved in Trizol (Takara Biotechnology Co., Ltd., Dalian, China) and treated 3 times with liquid nitrogen for 5 min and then a 37°C water bath for 5 min. Total RNA was extracted from the tissues according to the manufacturer's guidelines.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Fold decrements of template DNA of the β-globin and rhodopsin genes after MNase digestion of chromatin samples quantified by qPCR. Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .

    Journal: Nucleic Acids Research

    Article Title: Linker histone partial phosphorylation: effects on secondary structure and chromatin condensation

    doi: 10.1093/nar/gkv304

    Figure Lengend Snippet: Fold decrements of template DNA of the β-globin and rhodopsin genes after MNase digestion of chromatin samples quantified by qPCR. Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .

    Article Snippet: Purified DNA was used as a template for the amplification of β-globin and rhodopsin genes by Quantitative Polymerase Chain Reaction (qPCR), following MIQE guidelines, in a C1000TM thermal cycler (BioRad).

    Techniques: Real-time Polymerase Chain Reaction

    Zinc finger protein 407 (Zfp407) mRNA overexpression in ZFP-TG mice. A : transgene construct with mouse Zfp407 cDNA tagged with Myc and DDK. B – D : endogenous ( B ), transgenic ( C ), and total Zfp407 mRNA expression ( D ) were measured by quantitative PCR and normalized to levels of Arbp in wild-type (WT) and ZFP-TG (TG) mice ( n = 4–10 mice/group). * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Zinc finger protein 407 overexpression upregulates PPAR target gene expression and improves glucose homeostasis in mice

    doi: 10.1152/ajpendo.00234.2016

    Figure Lengend Snippet: Zinc finger protein 407 (Zfp407) mRNA overexpression in ZFP-TG mice. A : transgene construct with mouse Zfp407 cDNA tagged with Myc and DDK. B – D : endogenous ( B ), transgenic ( C ), and total Zfp407 mRNA expression ( D ) were measured by quantitative PCR and normalized to levels of Arbp in wild-type (WT) and ZFP-TG (TG) mice ( n = 4–10 mice/group). * P

    Article Snippet: Quantitative PCR (qPCR) reactions were performed with the power SYBR green PCR Master Mix and run on a Bio-Rad (Hercules, CA) CFX Connect Real Time System.

    Techniques: Over Expression, Mouse Assay, Construct, Transgenic Assay, Expressing, Real-time Polymerase Chain Reaction

    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Journal: Carcinogenesis

    Article Title: Flaxseed lignans enriched in secoisolariciresinol diglucoside prevent acute asbestos-induced peritoneal inflammation in mice

    doi: 10.1093/carcin/bgv174

    Figure Lengend Snippet: Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Article Snippet: Reverse transcription of RNA to cDNA was then performed on a Veriti® Thermal Cycler using the high capacity RNA to cDNA kit supplied by Applied Biosystems Quantitative polymerase chain reaction (qPCR) and performed using TaqMan® probe-based gene expression assays supplied by Applied Biosystems, Life Technologies (Carlsbad, CA).

    Techniques: Expressing, CTL Assay, Real-time Polymerase Chain Reaction

    Flaxseed lignan diet abrogates increased nitrosative and oxidative stress from exposure to asbestos fibers. Mouse cohorts (13 weeks old) were fed CTL ( n = 16) or FLC ( n = 17) diets for 7 days prior to exposure to 400 µg of crocidolite asbestos fibers. PLF was evaluated 3 days following asbestos exposure for nitrite ( A ) and MDA concentrations ( C ). PLF WBC mRNA expression of iNOS ( B ), HO-1 ( D ), Nqo1 ( E ) and Gstm1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Journal: Carcinogenesis

    Article Title: Flaxseed lignans enriched in secoisolariciresinol diglucoside prevent acute asbestos-induced peritoneal inflammation in mice

    doi: 10.1093/carcin/bgv174

    Figure Lengend Snippet: Flaxseed lignan diet abrogates increased nitrosative and oxidative stress from exposure to asbestos fibers. Mouse cohorts (13 weeks old) were fed CTL ( n = 16) or FLC ( n = 17) diets for 7 days prior to exposure to 400 µg of crocidolite asbestos fibers. PLF was evaluated 3 days following asbestos exposure for nitrite ( A ) and MDA concentrations ( C ). PLF WBC mRNA expression of iNOS ( B ), HO-1 ( D ), Nqo1 ( E ) and Gstm1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Article Snippet: Reverse transcription of RNA to cDNA was then performed on a Veriti® Thermal Cycler using the high capacity RNA to cDNA kit supplied by Applied Biosystems Quantitative polymerase chain reaction (qPCR) and performed using TaqMan® probe-based gene expression assays supplied by Applied Biosystems, Life Technologies (Carlsbad, CA).

    Techniques: CTL Assay, Multiple Displacement Amplification, Expressing, Real-time Polymerase Chain Reaction

    Flaxseed lignan diet ameliorates asbestos-induced WBC proinflammatory cytokine release and gene expression levels. PLF levels (pg/ml for IL-1ß and IL-6 and ng/ml for HMGB1) of proinflammatory cytokines IL-1ß ( A ), IL-6 ( C ) and HMGB1 ( E ) were evaluated 3 days post asbestos exposure. PLF WBC mRNA expression of IL-1ß ( B ), IL-6 ( D ) and HMGB1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Journal: Carcinogenesis

    Article Title: Flaxseed lignans enriched in secoisolariciresinol diglucoside prevent acute asbestos-induced peritoneal inflammation in mice

    doi: 10.1093/carcin/bgv174

    Figure Lengend Snippet: Flaxseed lignan diet ameliorates asbestos-induced WBC proinflammatory cytokine release and gene expression levels. PLF levels (pg/ml for IL-1ß and IL-6 and ng/ml for HMGB1) of proinflammatory cytokines IL-1ß ( A ), IL-6 ( C ) and HMGB1 ( E ) were evaluated 3 days post asbestos exposure. PLF WBC mRNA expression of IL-1ß ( B ), IL-6 ( D ) and HMGB1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Article Snippet: Reverse transcription of RNA to cDNA was then performed on a Veriti® Thermal Cycler using the high capacity RNA to cDNA kit supplied by Applied Biosystems Quantitative polymerase chain reaction (qPCR) and performed using TaqMan® probe-based gene expression assays supplied by Applied Biosystems, Life Technologies (Carlsbad, CA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, CTL Assay

    Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) qRT-PCR analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P

    Journal: Nucleic Acids Research

    Article Title: Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression

    doi: 10.1093/nar/gkw583

    Figure Lengend Snippet: Establishment of the CRISPR interference system in THP-1 acute monocytic leukemia cells. ( A ) Flow cytometry analysis of mCHERRY expression in THP-1;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting as well as after additional 3 months in culture. ( B ) Western blot analysis of dCas9 protein expression in THP-1;KRAB-dCas9-2A-CHERRY cells after 3 months in culture. dCas9 is detected using an antibody against the C-terminal HA-tag. ( C ) Experimental design used to assess the efficiency of individual sgRNAs to mediate knockdown of target gene expression. ( D ) qRT-PCR analysis of GTF2B and ANAPC4 expression in THP-1;KRAB-dCas9-2A-CHERRY cells transduced with either a non-targeting (negative control) sgRNA or sgRNAs against ANAPC4 and GTF2B , respectively. The values are normalized to RPLP0 and shown as mean ± SD. P -values ( P

    Article Snippet: Quantitative polymerase chain reaction (PCR) with reverse transcription (qRT-PCR) reactions were set up in triplicate using LightCycler 480 SYBR Green I Master (Roche) and primers listed in Supplementary Table S1. qRT-PCR experiments were performed on a LightCycler 480 Instrument II (Roche).

    Techniques: CRISPR, Flow Cytometry, Cytometry, Expressing, Transduction, FACS, Western Blot, Quantitative RT-PCR, Negative Control

    Comparison of the original and re-designed sgRNA sets targeting 20 genes chosen for re-design experiments. ( A ) Bar plot representing the number of functional and non-functional sgRNAs in the original and re-designed sgRNA sets. ( B ) Box plot comparing the proportion remaining transcript between the original and re-designed sgRNA sets. The lower and upper whiskers represent values for 5 and 95% of the data, respectively. The box designates lower and upper quantiles as well as a median value. The P -value is calculated using the Wilcoxon test. ( C ) Flow cytometry analysis of mCHERRY expression in BJ-hTERT/small-t;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting. ( D ) qRT-PCR analysis of the expression of ASH1L, KDM5B, PRDM2, SETD1B, SETD4, KDM6B, SETD7, SETD1A and KDM2A genes in BJ-hTERT/small-t;dCas9 immortalized human fibroblasts after the transduction with the indicated sgRNAs. Values represent mean ± SD. The location of the sgRNAs relative to the predominant CAGE-defined TSS is indicated in red in brackets. ( E ) Box plot comparing the SSC scores for functional and non-functional sgRNAs mapping inside and outside the area of −50…+150 bps around the predominant CAGE-peak center. The lower and upper whiskers represent values for 5 and 95% of the data, respectively. The box designates lower and upper quantiles as well as a median value. The P -values are calculated using the Wilcoxon test.

    Journal: Nucleic Acids Research

    Article Title: Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression

    doi: 10.1093/nar/gkw583

    Figure Lengend Snippet: Comparison of the original and re-designed sgRNA sets targeting 20 genes chosen for re-design experiments. ( A ) Bar plot representing the number of functional and non-functional sgRNAs in the original and re-designed sgRNA sets. ( B ) Box plot comparing the proportion remaining transcript between the original and re-designed sgRNA sets. The lower and upper whiskers represent values for 5 and 95% of the data, respectively. The box designates lower and upper quantiles as well as a median value. The P -value is calculated using the Wilcoxon test. ( C ) Flow cytometry analysis of mCHERRY expression in BJ-hTERT/small-t;dCas9-KRAB-2A-CHERRY cells after transduction and cell sorting. ( D ) qRT-PCR analysis of the expression of ASH1L, KDM5B, PRDM2, SETD1B, SETD4, KDM6B, SETD7, SETD1A and KDM2A genes in BJ-hTERT/small-t;dCas9 immortalized human fibroblasts after the transduction with the indicated sgRNAs. Values represent mean ± SD. The location of the sgRNAs relative to the predominant CAGE-defined TSS is indicated in red in brackets. ( E ) Box plot comparing the SSC scores for functional and non-functional sgRNAs mapping inside and outside the area of −50…+150 bps around the predominant CAGE-peak center. The lower and upper whiskers represent values for 5 and 95% of the data, respectively. The box designates lower and upper quantiles as well as a median value. The P -values are calculated using the Wilcoxon test.

    Article Snippet: Quantitative polymerase chain reaction (PCR) with reverse transcription (qRT-PCR) reactions were set up in triplicate using LightCycler 480 SYBR Green I Master (Roche) and primers listed in Supplementary Table S1. qRT-PCR experiments were performed on a LightCycler 480 Instrument II (Roche).

    Techniques: Functional Assay, Flow Cytometry, Cytometry, Expressing, Transduction, FACS, Quantitative RT-PCR