qpcr reaction Search Results


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  • 92
    Thermo Fisher quantitative polymerase chain reaction qpcr
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Quantitative Polymerase Chain Reaction Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1694 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche quantitative pcr analysis a lightcyler 96 quantitative polymerase chain reaction qpcr system
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Quantitative Pcr Analysis A Lightcyler 96 Quantitative Polymerase Chain Reaction Qpcr System, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad quantitative polymerase chain reaction qpcr quantitative pcr
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Quantitative Polymerase Chain Reaction Qpcr Quantitative Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche quantitative polymerase chain reaction qpcr
    Quantitative PCR results of the 3 confirmed <t>CNV</t> differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female
    Quantitative Polymerase Chain Reaction Qpcr, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative polymerase chain reaction qpcr
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Quantitative Polymerase Chain Reaction Qpcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative polymerase chain reaction pcr kits
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Quantitative Polymerase Chain Reaction Pcr Kits, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG fluorescence quantitative polymerase chain reaction pcr
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Fluorescence Quantitative Polymerase Chain Reaction Pcr, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG fluorescent quantitative polymerase chain reaction pcr
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Fluorescent Quantitative Polymerase Chain Reaction Pcr, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler quantitative polymerase chain reaction pcr
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Lightcycler Quantitative Polymerase Chain Reaction Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative real time polymerase chain reaction pcr quantitative real time polymerase chain reaction
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Quantitative Real Time Polymerase Chain Reaction Pcr Quantitative Real Time Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative polymerase chain reaction qpcr kit
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Quantitative Polymerase Chain Reaction Qpcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene quantitative polymerase chain reaction qpcr machine
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Quantitative Polymerase Chain Reaction Qpcr Machine, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kapa Biosystems quantitative polymerase chain reaction qpcr reagent
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Quantitative Polymerase Chain Reaction Qpcr Reagent, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7900ht quantitative polymerase chain reaction pcr instrument
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    7900ht Quantitative Polymerase Chain Reaction Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fluorescence quantitative polymerase chain reaction pcr instrument
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Fluorescence Quantitative Polymerase Chain Reaction Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore quantitative polymerase chain reaction qpcr primers
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Quantitative Polymerase Chain Reaction Qpcr Primers, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative polymerase chain reaction qpcr kits
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Quantitative Polymerase Chain Reaction Qpcr Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co quantitative polymerase chain reaction qpcr kit
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Quantitative Polymerase Chain Reaction Qpcr Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad fluorescence quantitative polymerase chain reaction pcr instrument
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Fluorescence Quantitative Polymerase Chain Reaction Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shanghai Kehua fluorescence quantitative polymerase chain reaction fq pcr
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Fluorescence Quantitative Polymerase Chain Reaction Fq Pcr, supplied by Shanghai Kehua, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad myiq quantitative polymerase chain reaction pcr machine
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Myiq Quantitative Polymerase Chain Reaction Pcr Machine, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fluorescence quantitative polymerase chain reaction pcr kit
    <t>miR-296</t> mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
    Fluorescence Quantitative Polymerase Chain Reaction Pcr Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Journal: Carcinogenesis

    Article Title: Flaxseed lignans enriched in secoisolariciresinol diglucoside prevent acute asbestos-induced peritoneal inflammation in mice

    doi: 10.1093/carcin/bgv174

    Figure Lengend Snippet: Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Article Snippet: Reverse transcription of RNA to cDNA was then performed on a Veriti® Thermal Cycler using the high capacity RNA to cDNA kit supplied by Applied Biosystems Quantitative polymerase chain reaction (qPCR) and performed using TaqMan® probe-based gene expression assays supplied by Applied Biosystems, Life Technologies (Carlsbad, CA).

    Techniques: Expressing, CTL Assay, Real-time Polymerase Chain Reaction

    Flaxseed lignan diet abrogates increased nitrosative and oxidative stress from exposure to asbestos fibers. Mouse cohorts (13 weeks old) were fed CTL ( n = 16) or FLC ( n = 17) diets for 7 days prior to exposure to 400 µg of crocidolite asbestos fibers. PLF was evaluated 3 days following asbestos exposure for nitrite ( A ) and MDA concentrations ( C ). PLF WBC mRNA expression of iNOS ( B ), HO-1 ( D ), Nqo1 ( E ) and Gstm1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Journal: Carcinogenesis

    Article Title: Flaxseed lignans enriched in secoisolariciresinol diglucoside prevent acute asbestos-induced peritoneal inflammation in mice

    doi: 10.1093/carcin/bgv174

    Figure Lengend Snippet: Flaxseed lignan diet abrogates increased nitrosative and oxidative stress from exposure to asbestos fibers. Mouse cohorts (13 weeks old) were fed CTL ( n = 16) or FLC ( n = 17) diets for 7 days prior to exposure to 400 µg of crocidolite asbestos fibers. PLF was evaluated 3 days following asbestos exposure for nitrite ( A ) and MDA concentrations ( C ). PLF WBC mRNA expression of iNOS ( B ), HO-1 ( D ), Nqo1 ( E ) and Gstm1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Article Snippet: Reverse transcription of RNA to cDNA was then performed on a Veriti® Thermal Cycler using the high capacity RNA to cDNA kit supplied by Applied Biosystems Quantitative polymerase chain reaction (qPCR) and performed using TaqMan® probe-based gene expression assays supplied by Applied Biosystems, Life Technologies (Carlsbad, CA).

    Techniques: CTL Assay, Multiple Displacement Amplification, Expressing, Real-time Polymerase Chain Reaction

    Flaxseed lignan diet ameliorates asbestos-induced WBC proinflammatory cytokine release and gene expression levels. PLF levels (pg/ml for IL-1ß and IL-6 and ng/ml for HMGB1) of proinflammatory cytokines IL-1ß ( A ), IL-6 ( C ) and HMGB1 ( E ) were evaluated 3 days post asbestos exposure. PLF WBC mRNA expression of IL-1ß ( B ), IL-6 ( D ) and HMGB1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Journal: Carcinogenesis

    Article Title: Flaxseed lignans enriched in secoisolariciresinol diglucoside prevent acute asbestos-induced peritoneal inflammation in mice

    doi: 10.1093/carcin/bgv174

    Figure Lengend Snippet: Flaxseed lignan diet ameliorates asbestos-induced WBC proinflammatory cytokine release and gene expression levels. PLF levels (pg/ml for IL-1ß and IL-6 and ng/ml for HMGB1) of proinflammatory cytokines IL-1ß ( A ), IL-6 ( C ) and HMGB1 ( E ) were evaluated 3 days post asbestos exposure. PLF WBC mRNA expression of IL-1ß ( B ), IL-6 ( D ) and HMGB1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Article Snippet: Reverse transcription of RNA to cDNA was then performed on a Veriti® Thermal Cycler using the high capacity RNA to cDNA kit supplied by Applied Biosystems Quantitative polymerase chain reaction (qPCR) and performed using TaqMan® probe-based gene expression assays supplied by Applied Biosystems, Life Technologies (Carlsbad, CA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, CTL Assay

    Fold decrements of template DNA of the β-globin and rhodopsin genes after MNase digestion of chromatin samples quantified by qPCR. Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .

    Journal: Nucleic Acids Research

    Article Title: Linker histone partial phosphorylation: effects on secondary structure and chromatin condensation

    doi: 10.1093/nar/gkv304

    Figure Lengend Snippet: Fold decrements of template DNA of the β-globin and rhodopsin genes after MNase digestion of chromatin samples quantified by qPCR. Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .

    Article Snippet: Purified DNA was used as a template for the amplification of β-globin and rhodopsin genes by Quantitative Polymerase Chain Reaction (qPCR), following MIQE guidelines, in a C1000TM thermal cycler (BioRad).

    Techniques: Real-time Polymerase Chain Reaction

    Quantitative PCR results of the 3 confirmed CNV differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female

    Journal: Molecular Syndromology

    Article Title: Differences in Copy Number Variation between Discordant Monozygotic Twins as a Model for Exploring Chromosomal Mosaicism in Congenital Heart Defects

    doi: 10.1159/000335284

    Figure Lengend Snippet: Quantitative PCR results of the 3 confirmed CNV differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female

    Article Snippet: To confirm the presence of CNV differences, quantitative polymerase chain reaction (qPCR) was performed on DNA from both twin members and 3 unrelated controls (2 males and 1 female) using the LightCycler® 480 Real-Time PCR system (Roche Applied Science, Belgium, ), following the manufacturer's instructions.

    Techniques: Real-time Polymerase Chain Reaction

    miR-296 mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after alkali exposures

    doi: 10.3892/etm.2017.5408

    Figure Lengend Snippet: miR-296 mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P

    Article Snippet: Total RNA sample preparation and quantitative polymerase chain reaction (qPCR) To quantify miR-296 mRNA expression levels, frozen mice corneal tissues were dissolved in Trizol (Takara Biotechnology Co., Ltd., Dalian, China) and treated 3 times with liquid nitrogen for 5 min and then a 37°C water bath for 5 min. Total RNA was extracted from the tissues according to the manufacturer's guidelines.

    Techniques: Expressing, Real-time Polymerase Chain Reaction