Journal: Experimental and Therapeutic Medicine
Article Title: MicroRNA-296 mediated corneal neovascularization in an animal model of corneal burns after alkali exposures
Figure Lengend Snippet: miR-296 mRNA expressed at different levels in different treatment on day 7 after corneal alkali burn. Histogram showing miR-296 mRNA expression level as determined by quantitative polymerase chain reaction. The y-axis shows miR-296 expression levels relative to the β-actin reference gene. Data shown are based on the linear conversion of ΔCq values for each sample (n=3, error bars denote standard error of the mean; *P
Article Snippet: Total RNA sample preparation and quantitative polymerase chain reaction (qPCR) To quantify miR-296 mRNA expression levels, frozen mice corneal tissues were dissolved in Trizol (Takara Biotechnology Co., Ltd., Dalian, China) and treated 3 times with liquid nitrogen for 5 min and then a 37°C water bath for 5 min. Total RNA was extracted from the tissues according to the manufacturer's guidelines.
Techniques: Expressing, Real-time Polymerase Chain Reaction