Journal: International Journal of Molecular Sciences

Article Title: Is Cadmium Toxicity Tissue-Specific? Toxicogenomics Studies Reveal Common and Specific Pathways in Pulmonary, Hepatic, and Neuronal Cell Models

doi: 10.3390/ijms23031768

Figure Lengend Snippet: Relative quantification of HMOX1, GADD45β, SLC30A1 and SLC30A2 mRNA levels by real time quantitative PCR. The relative expression levels in A549 ( A ) and SH-SY5Y ( B ) cells exposed to 10 μM and 20 μM Cd for 24 h were expressed as a fold change ± standard error (SE), using β-ACT gene as internal reference, and cells not treated with cadmium as calibrator. Values are presented as means ± standard error (SE) of three different experiments. Significantly different from control: *** p < 0.001 (Dunnett’s test).

Article Snippet: Briefly, 50 ng cDNA was PCR amplified with Luna ® Universal qPCR Master Mix (New England BioLabs, Hitchin, Hertfordshire, UK) and specific primers, using an initial denaturation step at 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 sec and 59 °C annealing for 1 min. Each sample was normalized using β-actin gene as an internal reference control.

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: medRxiv

Article Title: Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA

doi: 10.1101/2020.04.18.20070755

Figure Lengend Snippet: Linearity of SARS-CoV-2 RT-qPCR by direct approach. A tittered SARS-CoV-2 positive nasopharyngeal specimen was serially diluted using a negative specimen, pre-heated at 65°C for 10 min and assessed by SARS-CoV-2 RT-qPCR as described in the Supplemental methods. A) Amplification curves from SARS-CoV-2 RT-qPCR assay on the serially diluted sample. B) RT-qPCR C T values were plotted against estimated copy number of SARS-CoV-2 RNA in each dilution. Each data point represents an average of data obtained from 8 replicates.

Article Snippet: Similar results were observed when two commercial test kits were used for direct RT-qPCR: Arcis Coronavirus RNA extraction research kit comes with lysis reagents that can be used directly in RT-qPCR; and Takara PrimeDirect™ Probe RT-qPCR kit provides a master mix that is compatible with heat-treated specimen extracts.

Techniques: Quantitative RT-PCR, Amplification

Journal: medRxiv

Article Title: Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA

doi: 10.1101/2020.04.18.20070755

Figure Lengend Snippet: Correlation of RT=qPCR C T values obtained by direct versus standard approach.

Article Snippet: Similar results were observed when two commercial test kits were used for direct RT-qPCR: Arcis Coronavirus RNA extraction research kit comes with lysis reagents that can be used directly in RT-qPCR; and Takara PrimeDirect™ Probe RT-qPCR kit provides a master mix that is compatible with heat-treated specimen extracts.

Techniques: Quantitative RT-PCR