qpcr instrument Search Results


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  • 99
    Thermo Fisher 7900ht quantitative pcr instrument
    7900ht Quantitative Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7900ht quantitative pcr instrument/product/Thermo Fisher
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    7900ht quantitative pcr instrument - by Bioz Stars, 2020-09
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    91
    Thermo Fisher qpcr instrument
    Assay LODs using model CTC samples. Varying levels of KRAS <t>G13D</t> mutation-positive tumor cells (MDA-MB-231) were spiked into healthy donor blood and processed on the IsoFlux System. gDNA was amplified, purified, and tested for KRAS mutations. The C t difference between KRAS mutant and wild-type assays (d C t ) was calculated. Samples having as few as nine recovered tumor cells were detectable using the <t>qPCR</t> assay. Healthy control samples remained above the mutation detection cutoff.
    Qpcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 329 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qpcr instrument/product/Thermo Fisher
    Average 91 stars, based on 329 article reviews
    Price from $9.99 to $1999.99
    qpcr instrument - by Bioz Stars, 2020-09
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    90
    Thermo Fisher abi7500 quantitative pcr instrument
    Assay LODs using model CTC samples. Varying levels of KRAS <t>G13D</t> mutation-positive tumor cells (MDA-MB-231) were spiked into healthy donor blood and processed on the IsoFlux System. gDNA was amplified, purified, and tested for KRAS mutations. The C t difference between KRAS mutant and wild-type assays (d C t ) was calculated. Samples having as few as nine recovered tumor cells were detectable using the <t>qPCR</t> assay. Healthy control samples remained above the mutation detection cutoff.
    Abi7500 Quantitative Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abi7500 quantitative pcr instrument/product/Thermo Fisher
    Average 90 stars, based on 154 article reviews
    Price from $9.99 to $1999.99
    abi7500 quantitative pcr instrument - by Bioz Stars, 2020-09
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    89
    Bio-Rad cfx96 qpcr instrument
    Assay LODs using model CTC samples. Varying levels of KRAS <t>G13D</t> mutation-positive tumor cells (MDA-MB-231) were spiked into healthy donor blood and processed on the IsoFlux System. gDNA was amplified, purified, and tested for KRAS mutations. The C t difference between KRAS mutant and wild-type assays (d C t ) was calculated. Samples having as few as nine recovered tumor cells were detectable using the <t>qPCR</t> assay. Healthy control samples remained above the mutation detection cutoff.
    Cfx96 Qpcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cfx96 qpcr instrument/product/Bio-Rad
    Average 89 stars, based on 252 article reviews
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    90
    Bio-Rad qpcr instrument
    gD facilitates IRF7-mediated <t>IFN-β</t> promoter activity through enhancing the interaction of viperin with IRAK1 and increasing K63-linked polyubiquitination of IRAK1. (A–C) HEK293T cells were co-transfected with IFN-β-Luc reporter, pRL-TK and gD-EYFP or pViperin-Flag or plasmids combination of gD-EYFP and pViperin-Flag, with or without IRF3/5D (A) or IRF7/6D (B,C) expression plasmid. Twenty-four hours post-transfection, luciferase activity was analyzed. (D) HEK293T cells were co-transfected with the indicated plasmids as described in (C) , except for the reporter plasmids. Twenty-four hours post-transfection, <t>RT-qPCR</t> was performed to analyze the relative mRNA expression level of IFN-β. Data were expressed as means ± SD from three independent experiments. (E,F) HEK293T cells co-transfected with expression plasmids pViperin-Flag, IRAK1-HA and gD-EYFP, or EYFP control construct were harvested and immunoprecipitated with mouse anti-Flag mAb or non-specific mouse IgG, and IB analysis was probed with the indicated antibodies. Densitometry of the IRAK1 and viperin interaction bands were normalized to the loading control β-actin. (G,H) HA-tagged Ub (WT), Ub (K48), or Ub (K63) expression plasmid was co-transfected with plasmids combination of pCMV-Flag-IRAK1 and pViperin-Flag into HEK293T cells, with or without the presence of gD-EYFP. Twenty-four hours post-transfection, cells were collected, followed by Co-IP with mouse anti-Flag mAb and IB analysis with mouse anti-HA mAb. Densitometry of IRAK1 polyubiquitination bands were normalized to the loading control β-actin. Data were expressed as means ± SD from three independent experiments. Statistical analyses were performed using one-way ANOVA, except (F) using student t test. * P
    Qpcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qpcr instrument/product/Bio-Rad
    Average 90 stars, based on 74 article reviews
    Price from $9.99 to $1999.99
    qpcr instrument - by Bioz Stars, 2020-09
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    90
    Stratagene mx3000p qpcr instrument
    gD facilitates IRF7-mediated <t>IFN-β</t> promoter activity through enhancing the interaction of viperin with IRAK1 and increasing K63-linked polyubiquitination of IRAK1. (A–C) HEK293T cells were co-transfected with IFN-β-Luc reporter, pRL-TK and gD-EYFP or pViperin-Flag or plasmids combination of gD-EYFP and pViperin-Flag, with or without IRF3/5D (A) or IRF7/6D (B,C) expression plasmid. Twenty-four hours post-transfection, luciferase activity was analyzed. (D) HEK293T cells were co-transfected with the indicated plasmids as described in (C) , except for the reporter plasmids. Twenty-four hours post-transfection, <t>RT-qPCR</t> was performed to analyze the relative mRNA expression level of IFN-β. Data were expressed as means ± SD from three independent experiments. (E,F) HEK293T cells co-transfected with expression plasmids pViperin-Flag, IRAK1-HA and gD-EYFP, or EYFP control construct were harvested and immunoprecipitated with mouse anti-Flag mAb or non-specific mouse IgG, and IB analysis was probed with the indicated antibodies. Densitometry of the IRAK1 and viperin interaction bands were normalized to the loading control β-actin. (G,H) HA-tagged Ub (WT), Ub (K48), or Ub (K63) expression plasmid was co-transfected with plasmids combination of pCMV-Flag-IRAK1 and pViperin-Flag into HEK293T cells, with or without the presence of gD-EYFP. Twenty-four hours post-transfection, cells were collected, followed by Co-IP with mouse anti-Flag mAb and IB analysis with mouse anti-HA mAb. Densitometry of IRAK1 polyubiquitination bands were normalized to the loading control β-actin. Data were expressed as means ± SD from three independent experiments. Statistical analyses were performed using one-way ANOVA, except (F) using student t test. * P
    Mx3000p Qpcr Instrument, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mx3000p qpcr instrument/product/Stratagene
    Average 90 stars, based on 234 article reviews
    Price from $9.99 to $1999.99
    mx3000p qpcr instrument - by Bioz Stars, 2020-09
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    90
    Thermo Fisher abi 7500 fluorescence quantitative pcr instrument
    gD facilitates IRF7-mediated <t>IFN-β</t> promoter activity through enhancing the interaction of viperin with IRAK1 and increasing K63-linked polyubiquitination of IRAK1. (A–C) HEK293T cells were co-transfected with IFN-β-Luc reporter, pRL-TK and gD-EYFP or pViperin-Flag or plasmids combination of gD-EYFP and pViperin-Flag, with or without IRF3/5D (A) or IRF7/6D (B,C) expression plasmid. Twenty-four hours post-transfection, luciferase activity was analyzed. (D) HEK293T cells were co-transfected with the indicated plasmids as described in (C) , except for the reporter plasmids. Twenty-four hours post-transfection, <t>RT-qPCR</t> was performed to analyze the relative mRNA expression level of IFN-β. Data were expressed as means ± SD from three independent experiments. (E,F) HEK293T cells co-transfected with expression plasmids pViperin-Flag, IRAK1-HA and gD-EYFP, or EYFP control construct were harvested and immunoprecipitated with mouse anti-Flag mAb or non-specific mouse IgG, and IB analysis was probed with the indicated antibodies. Densitometry of the IRAK1 and viperin interaction bands were normalized to the loading control β-actin. (G,H) HA-tagged Ub (WT), Ub (K48), or Ub (K63) expression plasmid was co-transfected with plasmids combination of pCMV-Flag-IRAK1 and pViperin-Flag into HEK293T cells, with or without the presence of gD-EYFP. Twenty-four hours post-transfection, cells were collected, followed by Co-IP with mouse anti-Flag mAb and IB analysis with mouse anti-HA mAb. Densitometry of IRAK1 polyubiquitination bands were normalized to the loading control β-actin. Data were expressed as means ± SD from three independent experiments. Statistical analyses were performed using one-way ANOVA, except (F) using student t test. * P
    Abi 7500 Fluorescence Quantitative Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
    abi 7500 fluorescence quantitative pcr instrument - by Bioz Stars, 2020-09
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    91
    Agilent technologies mx3005p qpcr instrument
    gD facilitates IRF7-mediated <t>IFN-β</t> promoter activity through enhancing the interaction of viperin with IRAK1 and increasing K63-linked polyubiquitination of IRAK1. (A–C) HEK293T cells were co-transfected with IFN-β-Luc reporter, pRL-TK and gD-EYFP or pViperin-Flag or plasmids combination of gD-EYFP and pViperin-Flag, with or without IRF3/5D (A) or IRF7/6D (B,C) expression plasmid. Twenty-four hours post-transfection, luciferase activity was analyzed. (D) HEK293T cells were co-transfected with the indicated plasmids as described in (C) , except for the reporter plasmids. Twenty-four hours post-transfection, <t>RT-qPCR</t> was performed to analyze the relative mRNA expression level of IFN-β. Data were expressed as means ± SD from three independent experiments. (E,F) HEK293T cells co-transfected with expression plasmids pViperin-Flag, IRAK1-HA and gD-EYFP, or EYFP control construct were harvested and immunoprecipitated with mouse anti-Flag mAb or non-specific mouse IgG, and IB analysis was probed with the indicated antibodies. Densitometry of the IRAK1 and viperin interaction bands were normalized to the loading control β-actin. (G,H) HA-tagged Ub (WT), Ub (K48), or Ub (K63) expression plasmid was co-transfected with plasmids combination of pCMV-Flag-IRAK1 and pViperin-Flag into HEK293T cells, with or without the presence of gD-EYFP. Twenty-four hours post-transfection, cells were collected, followed by Co-IP with mouse anti-Flag mAb and IB analysis with mouse anti-HA mAb. Densitometry of IRAK1 polyubiquitination bands were normalized to the loading control β-actin. Data were expressed as means ± SD from three independent experiments. Statistical analyses were performed using one-way ANOVA, except (F) using student t test. * P
    Mx3005p Qpcr Instrument, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mx3005p qpcr instrument/product/Agilent technologies
    Average 91 stars, based on 204 article reviews
    Price from $9.99 to $1999.99
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    88
    Stratagene mx3005p quantitative pcr instrument
    gD facilitates IRF7-mediated <t>IFN-β</t> promoter activity through enhancing the interaction of viperin with IRAK1 and increasing K63-linked polyubiquitination of IRAK1. (A–C) HEK293T cells were co-transfected with IFN-β-Luc reporter, pRL-TK and gD-EYFP or pViperin-Flag or plasmids combination of gD-EYFP and pViperin-Flag, with or without IRF3/5D (A) or IRF7/6D (B,C) expression plasmid. Twenty-four hours post-transfection, luciferase activity was analyzed. (D) HEK293T cells were co-transfected with the indicated plasmids as described in (C) , except for the reporter plasmids. Twenty-four hours post-transfection, <t>RT-qPCR</t> was performed to analyze the relative mRNA expression level of IFN-β. Data were expressed as means ± SD from three independent experiments. (E,F) HEK293T cells co-transfected with expression plasmids pViperin-Flag, IRAK1-HA and gD-EYFP, or EYFP control construct were harvested and immunoprecipitated with mouse anti-Flag mAb or non-specific mouse IgG, and IB analysis was probed with the indicated antibodies. Densitometry of the IRAK1 and viperin interaction bands were normalized to the loading control β-actin. (G,H) HA-tagged Ub (WT), Ub (K48), or Ub (K63) expression plasmid was co-transfected with plasmids combination of pCMV-Flag-IRAK1 and pViperin-Flag into HEK293T cells, with or without the presence of gD-EYFP. Twenty-four hours post-transfection, cells were collected, followed by Co-IP with mouse anti-Flag mAb and IB analysis with mouse anti-HA mAb. Densitometry of IRAK1 polyubiquitination bands were normalized to the loading control β-actin. Data were expressed as means ± SD from three independent experiments. Statistical analyses were performed using one-way ANOVA, except (F) using student t test. * P
    Mx3005p Quantitative Pcr Instrument, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mx3005p quantitative pcr instrument/product/Stratagene
    Average 88 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    mx3005p quantitative pcr instrument - by Bioz Stars, 2020-09
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    90
    Thermo Fisher fluorescence quantitative pcr instrument
    gD facilitates IRF7-mediated <t>IFN-β</t> promoter activity through enhancing the interaction of viperin with IRAK1 and increasing K63-linked polyubiquitination of IRAK1. (A–C) HEK293T cells were co-transfected with IFN-β-Luc reporter, pRL-TK and gD-EYFP or pViperin-Flag or plasmids combination of gD-EYFP and pViperin-Flag, with or without IRF3/5D (A) or IRF7/6D (B,C) expression plasmid. Twenty-four hours post-transfection, luciferase activity was analyzed. (D) HEK293T cells were co-transfected with the indicated plasmids as described in (C) , except for the reporter plasmids. Twenty-four hours post-transfection, <t>RT-qPCR</t> was performed to analyze the relative mRNA expression level of IFN-β. Data were expressed as means ± SD from three independent experiments. (E,F) HEK293T cells co-transfected with expression plasmids pViperin-Flag, IRAK1-HA and gD-EYFP, or EYFP control construct were harvested and immunoprecipitated with mouse anti-Flag mAb or non-specific mouse IgG, and IB analysis was probed with the indicated antibodies. Densitometry of the IRAK1 and viperin interaction bands were normalized to the loading control β-actin. (G,H) HA-tagged Ub (WT), Ub (K48), or Ub (K63) expression plasmid was co-transfected with plasmids combination of pCMV-Flag-IRAK1 and pViperin-Flag into HEK293T cells, with or without the presence of gD-EYFP. Twenty-four hours post-transfection, cells were collected, followed by Co-IP with mouse anti-Flag mAb and IB analysis with mouse anti-HA mAb. Densitometry of IRAK1 polyubiquitination bands were normalized to the loading control β-actin. Data were expressed as means ± SD from three independent experiments. Statistical analyses were performed using one-way ANOVA, except (F) using student t test. * P
    Fluorescence Quantitative Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence quantitative pcr instrument/product/Thermo Fisher
    Average 90 stars, based on 93 article reviews
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    fluorescence quantitative pcr instrument - by Bioz Stars, 2020-09
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    90
    Bio-Rad fluorescence quantitative pcr instrument
    The DNA methylation level in the 5′ flanking region of <t>C3</t> and KNG1 after GCRV infection. ( a ) A schematic diagram of CpG loci in the 5′ flanking region of C3 and KNG1 genes. Four CpG loci were selected in both C3 (-293, -192, -164, and -132) and KNG1 (-215, -207, -115, and -104) genes for <t>BSP-PCR.</t> ( b ) Changes of DNA methylation levels after GCRV infection. Data are shown as the mean ± standard deviation of three biological duplicates ( n = 5 for each biological duplicate). Significant difference ( p
    Fluorescence Quantitative Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescence quantitative pcr instrument/product/Bio-Rad
    Average 90 stars, based on 161 article reviews
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    90
    Agilent technologies mx3000p qpcr instrument
    The DNA methylation level in the 5′ flanking region of <t>C3</t> and KNG1 after GCRV infection. ( a ) A schematic diagram of CpG loci in the 5′ flanking region of C3 and KNG1 genes. Four CpG loci were selected in both C3 (-293, -192, -164, and -132) and KNG1 (-215, -207, -115, and -104) genes for <t>BSP-PCR.</t> ( b ) Changes of DNA methylation levels after GCRV infection. Data are shown as the mean ± standard deviation of three biological duplicates ( n = 5 for each biological duplicate). Significant difference ( p
    Mx3000p Qpcr Instrument, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mx3000p qpcr instrument/product/Agilent technologies
    Average 90 stars, based on 118 article reviews
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    88
    Thermo Fisher viia7 qpcr instrument
    Details of the PCR setup. Detailed information of the PCR setup on the <t>ViiA7</t> instrument with telomere primers ( A ; annealing at 56 °C) and human β-globin primers ( B ; annealing at 58 °C) is provided. Cycling temperatures with time, number of cycles, as well as ramping rates are clearly indicated here. A melt curve analysis is added at the end of 40 cycles of PCR to ensure the absence of primer-dimers.
    Viia7 Qpcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 141 article reviews
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    88
    Thermo Fisher stepone qpcr instrument
    Genes significantly deregulated in HCT116 cells with corrected PRDM2 (A-B) Top 10 PRDM2-deregulated genes identified under normal (A) and reduced (B) serum conditions. (C-D) <t>RT-qPCR</t> validation of PRDM2-deregulated target genes identified under normal (C) and reduced (D) serum conditions. (E-F) RT-qPCR validation of PRDM2-deregulated genes involved in EMT under normal (E) and reduced (F) serum conditions. Genes validated in at least two of the three clones are shown. Bars represent minimum and maximum relative quantity (RQ) calculated by the <t>StepOne</t> software (Applied Biosystems). The dotted line indicates the level of gene expression in parental HCT116 cells. Data from one experiment are presented.
    Stepone Qpcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad cfx qpcr instrument
    Genes significantly deregulated in HCT116 cells with corrected PRDM2 (A-B) Top 10 PRDM2-deregulated genes identified under normal (A) and reduced (B) serum conditions. (C-D) <t>RT-qPCR</t> validation of PRDM2-deregulated target genes identified under normal (C) and reduced (D) serum conditions. (E-F) RT-qPCR validation of PRDM2-deregulated genes involved in EMT under normal (E) and reduced (F) serum conditions. Genes validated in at least two of the three clones are shown. Bars represent minimum and maximum relative quantity (RQ) calculated by the <t>StepOne</t> software (Applied Biosystems). The dotted line indicates the level of gene expression in parental HCT116 cells. Data from one experiment are presented.
    Cfx Qpcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher stepone plus qpcr instrument
    Genes significantly deregulated in HCT116 cells with corrected PRDM2 (A-B) Top 10 PRDM2-deregulated genes identified under normal (A) and reduced (B) serum conditions. (C-D) <t>RT-qPCR</t> validation of PRDM2-deregulated target genes identified under normal (C) and reduced (D) serum conditions. (E-F) RT-qPCR validation of PRDM2-deregulated genes involved in EMT under normal (E) and reduced (F) serum conditions. Genes validated in at least two of the three clones are shown. Bars represent minimum and maximum relative quantity (RQ) calculated by the <t>StepOne</t> software (Applied Biosystems). The dotted line indicates the level of gene expression in parental HCT116 cells. Data from one experiment are presented.
    Stepone Plus Qpcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher 7500 fast real time qpcr instrument
    Genes significantly deregulated in HCT116 cells with corrected PRDM2 (A-B) Top 10 PRDM2-deregulated genes identified under normal (A) and reduced (B) serum conditions. (C-D) <t>RT-qPCR</t> validation of PRDM2-deregulated target genes identified under normal (C) and reduced (D) serum conditions. (E-F) RT-qPCR validation of PRDM2-deregulated genes involved in EMT under normal (E) and reduced (F) serum conditions. Genes validated in at least two of the three clones are shown. Bars represent minimum and maximum relative quantity (RQ) calculated by the <t>StepOne</t> software (Applied Biosystems). The dotted line indicates the level of gene expression in parental HCT116 cells. Data from one experiment are presented.
    7500 Fast Real Time Qpcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher 7500 qpcr instrument
    Genes significantly deregulated in HCT116 cells with corrected PRDM2 (A-B) Top 10 PRDM2-deregulated genes identified under normal (A) and reduced (B) serum conditions. (C-D) <t>RT-qPCR</t> validation of PRDM2-deregulated target genes identified under normal (C) and reduced (D) serum conditions. (E-F) RT-qPCR validation of PRDM2-deregulated genes involved in EMT under normal (E) and reduced (F) serum conditions. Genes validated in at least two of the three clones are shown. Bars represent minimum and maximum relative quantity (RQ) calculated by the <t>StepOne</t> software (Applied Biosystems). The dotted line indicates the level of gene expression in parental HCT116 cells. Data from one experiment are presented.
    7500 Qpcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Assay LODs using model CTC samples. Varying levels of KRAS G13D mutation-positive tumor cells (MDA-MB-231) were spiked into healthy donor blood and processed on the IsoFlux System. gDNA was amplified, purified, and tested for KRAS mutations. The C t difference between KRAS mutant and wild-type assays (d C t ) was calculated. Samples having as few as nine recovered tumor cells were detectable using the qPCR assay. Healthy control samples remained above the mutation detection cutoff.

    Journal: Translational Oncology

    Article Title: Mutational Analysis of Circulating Tumor Cells Using a Novel Microfluidic Collection Device and qPCR Assay 1Mutational Analysis of Circulating Tumor Cells Using a Novel Microfluidic Collection Device and qPCR Assay 1 2

    doi:

    Figure Lengend Snippet: Assay LODs using model CTC samples. Varying levels of KRAS G13D mutation-positive tumor cells (MDA-MB-231) were spiked into healthy donor blood and processed on the IsoFlux System. gDNA was amplified, purified, and tested for KRAS mutations. The C t difference between KRAS mutant and wild-type assays (d C t ) was calculated. Samples having as few as nine recovered tumor cells were detectable using the qPCR assay. Healthy control samples remained above the mutation detection cutoff.

    Article Snippet: Amplified gDNA was purified and eluted in 50 µl of AE buffer (QIAamp DNA Micro Kit; Qiagen) and used for qPCR. gDNA was tested for mutations using a panel of KRAS mutation assays (nucleotide changes of c.34G > T, c.34G > A, c.34G > C, c.35G > T, c.35G > A, c.35G > C, and c.38G > A, corresponding to amino acid changes of G12C, G12S, G12R, G12V, G12D, G12A, and G13D) using Competitive Allele-Specific TaqMan PCR (castPCR) reagents on a qPCR instrument (StepOne Plus; Life Technologies Inc) according to the manufacturer's recommended protocol [ ].

    Techniques: Mutagenesis, Multiple Displacement Amplification, Amplification, Purification, Real-time Polymerase Chain Reaction

    Comparison of allele discrimination in KASP and Amplifluor-like systems using the same Master-mix. SNP genotyping was developed targeting SNPs in a barley collection, in the FT5 gene using KASP ( a ) and Amplifluor-like ( b ) systems; and in the PHY-C gene using the same KASP ( c ) and Amplifluor-like ( d ) systems. Blue and red dots indicate genotypes with homozygous alleles, green dots for heterozygous, pink dots and crosses for samples with no signals, and black dots and squares shows NTC, No template controls. X- and Y-axes show Relative amplification units, ΔRn, for FAM and HEX/VIC fluorescence signals, respectively, as determined by the qPCR instrument

    Journal: BMC Plant Biology

    Article Title: Advantages of Amplifluor-like SNP markers over KASP in plant genotyping

    doi: 10.1186/s12870-017-1197-x

    Figure Lengend Snippet: Comparison of allele discrimination in KASP and Amplifluor-like systems using the same Master-mix. SNP genotyping was developed targeting SNPs in a barley collection, in the FT5 gene using KASP ( a ) and Amplifluor-like ( b ) systems; and in the PHY-C gene using the same KASP ( c ) and Amplifluor-like ( d ) systems. Blue and red dots indicate genotypes with homozygous alleles, green dots for heterozygous, pink dots and crosses for samples with no signals, and black dots and squares shows NTC, No template controls. X- and Y-axes show Relative amplification units, ΔRn, for FAM and HEX/VIC fluorescence signals, respectively, as determined by the qPCR instrument

    Article Snippet: Compatibility of different qPCR instruments and fluorophores for allele discrimination No differences were found in the identical experiments of Amplifluor-like SNP genotyping with UPs labeled with FAM and VIC, using qPCR instruments manufactured by ThermoFisher and BioRad (Fig. ).

    Techniques: Amplification, Fluorescence, Real-time Polymerase Chain Reaction

    Examples of rare SNP alleles in bread wheat and barley originated from Kazakhstan. a Rare SNP allele 1 in the collection of Kazakh wheat (labelled with FAM). b Results of the discrimination of rare SNP allele 2 (labelled with VIC) in the Kazakh barley collection. X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals, respectively, as determined by the qPCR instrument

    Journal: BMC Plant Biology

    Article Title: Advantages of Amplifluor-like SNP markers over KASP in plant genotyping

    doi: 10.1186/s12870-017-1197-x

    Figure Lengend Snippet: Examples of rare SNP alleles in bread wheat and barley originated from Kazakhstan. a Rare SNP allele 1 in the collection of Kazakh wheat (labelled with FAM). b Results of the discrimination of rare SNP allele 2 (labelled with VIC) in the Kazakh barley collection. X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals, respectively, as determined by the qPCR instrument

    Article Snippet: Compatibility of different qPCR instruments and fluorophores for allele discrimination No differences were found in the identical experiments of Amplifluor-like SNP genotyping with UPs labeled with FAM and VIC, using qPCR instruments manufactured by ThermoFisher and BioRad (Fig. ).

    Techniques: Amplification, Fluorescence, Real-time Polymerase Chain Reaction

    Comparison of different concentrations of Taq -polymerase in PCR with Amplifluor-like SNP markers for ‘trouble-shooting’ of allele discrimination. The identicial PCR protocol was applied with the same DNA samples from a wheat collection from Kazakhstan and the same marker W58, using 0.5 units ( a ) or 0.1 units ( b ) of Maxima Hot-start Taq -polymerase (ThermoFisher, USA). X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals, respectively, as determined by the qPCR instrument

    Journal: BMC Plant Biology

    Article Title: Advantages of Amplifluor-like SNP markers over KASP in plant genotyping

    doi: 10.1186/s12870-017-1197-x

    Figure Lengend Snippet: Comparison of different concentrations of Taq -polymerase in PCR with Amplifluor-like SNP markers for ‘trouble-shooting’ of allele discrimination. The identicial PCR protocol was applied with the same DNA samples from a wheat collection from Kazakhstan and the same marker W58, using 0.5 units ( a ) or 0.1 units ( b ) of Maxima Hot-start Taq -polymerase (ThermoFisher, USA). X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals, respectively, as determined by the qPCR instrument

    Article Snippet: Compatibility of different qPCR instruments and fluorophores for allele discrimination No differences were found in the identical experiments of Amplifluor-like SNP genotyping with UPs labeled with FAM and VIC, using qPCR instruments manufactured by ThermoFisher and BioRad (Fig. ).

    Techniques: Polymerase Chain Reaction, Marker, Amplification, Fluorescence, Real-time Polymerase Chain Reaction

    Comparison of Amplifluor-like SNP marker application with the same UPs labelled with FAM and VIC fluorophores. Quant Studio-7 Real-Time PCR instrument (ThermoFisher Scientific, Paisley, UK), designed for FAM and VIC scoring ( a ) and Real-Time qPCR system, Model CFX96 (BioRad, Gladesville, NSW, Australia), designed for FAM and HEX discrimination ( b ), were used. Automatic calls for alleles are shown in different colours, using supplementary software for each instrument separately. X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals ( a ); and Relative fluorescence units, RFU, for FAM and HEX ( b ); as determined by the qPCR ThermoFisher and BioRad instruments, respectively

    Journal: BMC Plant Biology

    Article Title: Advantages of Amplifluor-like SNP markers over KASP in plant genotyping

    doi: 10.1186/s12870-017-1197-x

    Figure Lengend Snippet: Comparison of Amplifluor-like SNP marker application with the same UPs labelled with FAM and VIC fluorophores. Quant Studio-7 Real-Time PCR instrument (ThermoFisher Scientific, Paisley, UK), designed for FAM and VIC scoring ( a ) and Real-Time qPCR system, Model CFX96 (BioRad, Gladesville, NSW, Australia), designed for FAM and HEX discrimination ( b ), were used. Automatic calls for alleles are shown in different colours, using supplementary software for each instrument separately. X- and Y-axes show Relative amplification units, ΔRn, for FAM and VIC fluorescence signals ( a ); and Relative fluorescence units, RFU, for FAM and HEX ( b ); as determined by the qPCR ThermoFisher and BioRad instruments, respectively

    Article Snippet: Compatibility of different qPCR instruments and fluorophores for allele discrimination No differences were found in the identical experiments of Amplifluor-like SNP genotyping with UPs labeled with FAM and VIC, using qPCR instruments manufactured by ThermoFisher and BioRad (Fig. ).

    Techniques: Marker, Real-time Polymerase Chain Reaction, Software, Amplification, Fluorescence

    gD facilitates IRF7-mediated IFN-β promoter activity through enhancing the interaction of viperin with IRAK1 and increasing K63-linked polyubiquitination of IRAK1. (A–C) HEK293T cells were co-transfected with IFN-β-Luc reporter, pRL-TK and gD-EYFP or pViperin-Flag or plasmids combination of gD-EYFP and pViperin-Flag, with or without IRF3/5D (A) or IRF7/6D (B,C) expression plasmid. Twenty-four hours post-transfection, luciferase activity was analyzed. (D) HEK293T cells were co-transfected with the indicated plasmids as described in (C) , except for the reporter plasmids. Twenty-four hours post-transfection, RT-qPCR was performed to analyze the relative mRNA expression level of IFN-β. Data were expressed as means ± SD from three independent experiments. (E,F) HEK293T cells co-transfected with expression plasmids pViperin-Flag, IRAK1-HA and gD-EYFP, or EYFP control construct were harvested and immunoprecipitated with mouse anti-Flag mAb or non-specific mouse IgG, and IB analysis was probed with the indicated antibodies. Densitometry of the IRAK1 and viperin interaction bands were normalized to the loading control β-actin. (G,H) HA-tagged Ub (WT), Ub (K48), or Ub (K63) expression plasmid was co-transfected with plasmids combination of pCMV-Flag-IRAK1 and pViperin-Flag into HEK293T cells, with or without the presence of gD-EYFP. Twenty-four hours post-transfection, cells were collected, followed by Co-IP with mouse anti-Flag mAb and IB analysis with mouse anti-HA mAb. Densitometry of IRAK1 polyubiquitination bands were normalized to the loading control β-actin. Data were expressed as means ± SD from three independent experiments. Statistical analyses were performed using one-way ANOVA, except (F) using student t test. * P

    Journal: Frontiers in Immunology

    Article Title: The Interaction Mechanism Between Herpes Simplex Virus 1 Glycoprotein D and Host Antiviral Protein Viperin

    doi: 10.3389/fimmu.2019.02810

    Figure Lengend Snippet: gD facilitates IRF7-mediated IFN-β promoter activity through enhancing the interaction of viperin with IRAK1 and increasing K63-linked polyubiquitination of IRAK1. (A–C) HEK293T cells were co-transfected with IFN-β-Luc reporter, pRL-TK and gD-EYFP or pViperin-Flag or plasmids combination of gD-EYFP and pViperin-Flag, with or without IRF3/5D (A) or IRF7/6D (B,C) expression plasmid. Twenty-four hours post-transfection, luciferase activity was analyzed. (D) HEK293T cells were co-transfected with the indicated plasmids as described in (C) , except for the reporter plasmids. Twenty-four hours post-transfection, RT-qPCR was performed to analyze the relative mRNA expression level of IFN-β. Data were expressed as means ± SD from three independent experiments. (E,F) HEK293T cells co-transfected with expression plasmids pViperin-Flag, IRAK1-HA and gD-EYFP, or EYFP control construct were harvested and immunoprecipitated with mouse anti-Flag mAb or non-specific mouse IgG, and IB analysis was probed with the indicated antibodies. Densitometry of the IRAK1 and viperin interaction bands were normalized to the loading control β-actin. (G,H) HA-tagged Ub (WT), Ub (K48), or Ub (K63) expression plasmid was co-transfected with plasmids combination of pCMV-Flag-IRAK1 and pViperin-Flag into HEK293T cells, with or without the presence of gD-EYFP. Twenty-four hours post-transfection, cells were collected, followed by Co-IP with mouse anti-Flag mAb and IB analysis with mouse anti-HA mAb. Densitometry of IRAK1 polyubiquitination bands were normalized to the loading control β-actin. Data were expressed as means ± SD from three independent experiments. Statistical analyses were performed using one-way ANOVA, except (F) using student t test. * P

    Article Snippet: The acquired cDNA was taken as a template for qPCR, to detect the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (internal control) and IFN-β, using a qPCR instrument (BIO-RAD, CFX96).

    Techniques: Activity Assay, Transfection, Expressing, Plasmid Preparation, Luciferase, Quantitative RT-PCR, Construct, Immunoprecipitation, Co-Immunoprecipitation Assay

    PTEN was down-regulated in both MG -infected chicken embryo lung tissues and DF-1 cells. ( a ) The relative mRNA level of PTEN in MG infected chicken embryo lungs. Total RNA were extracted using TRNzol Universal from frozen infected chicken embryos lungs on days 5, 7, and 9 post-infection (equivalent to days 14, 16, and 18 of eggs hatching). Then, the relative mRNA level of PTEN in MG -infected lungs was detected by RT-qPCR. ( b ) The relative mRNA level of PTEN in MG -infected DF-1 cells. DF-1 cells cultured in six-well culture dishes were incubated with 200 μL MG (1 × 10 10 CCU/mL) for 24 h. Then, the cells were harvested. Total RNA of MG -infected DF-1 cells were extracted using TRNzol Universal. PTEN expression in infected DF-1 cells were detected by RT-qPCR. GAPDH was served as an internal control. Each experiment group contained at least three duplicates. Each duplicate was measured at least three times. Values are expressed as mean ± SD. Marked differences are expressed as * p

    Journal: International Journal of Molecular Sciences

    Article Title: Up-Regulation of miR-130b-3p Activates the PTEN/PI3K/AKT/NF-κB Pathway to Defense against Mycoplasma gallisepticum (HS Strain) Infection of Chicken

    doi: 10.3390/ijms19082172

    Figure Lengend Snippet: PTEN was down-regulated in both MG -infected chicken embryo lung tissues and DF-1 cells. ( a ) The relative mRNA level of PTEN in MG infected chicken embryo lungs. Total RNA were extracted using TRNzol Universal from frozen infected chicken embryos lungs on days 5, 7, and 9 post-infection (equivalent to days 14, 16, and 18 of eggs hatching). Then, the relative mRNA level of PTEN in MG -infected lungs was detected by RT-qPCR. ( b ) The relative mRNA level of PTEN in MG -infected DF-1 cells. DF-1 cells cultured in six-well culture dishes were incubated with 200 μL MG (1 × 10 10 CCU/mL) for 24 h. Then, the cells were harvested. Total RNA of MG -infected DF-1 cells were extracted using TRNzol Universal. PTEN expression in infected DF-1 cells were detected by RT-qPCR. GAPDH was served as an internal control. Each experiment group contained at least three duplicates. Each duplicate was measured at least three times. Values are expressed as mean ± SD. Marked differences are expressed as * p

    Article Snippet: Subsequently, the relative expression levels of miR-130b-3p, PTEN, PI3K, AKT, and NF-κB were detected by a real-time qPCR instrument (Bio-Rad, Hercules, CA, USA) with SuperReal PreMix Plus SYBR Green (TIANGEN, Beijing, China).

    Techniques: Infection, Quantitative RT-PCR, Cell Culture, Incubation, Expressing

    miR-130b-3p activates PI3K/AKT/NF-κB pathway. DF-1 cells cultured in six-well culture dishes were transfected with miR-130b-3p or miR-130b-3p-NC. After 48 h, the expressions of PI3K ( a ), AKT ( b ), and NF-κB ( c ) were examined by RT-qPCR. Similarly, DF-1 cells transfected with miR-130b-3p-Inh or miR-130b-3p-Inh-NC were incubated for 48 h. Then, the expressions of PI3K ( d ), AKT ( e ), and NF-κB ( f ) were examined through RT-qPCR. The relative expressions of PI3K, AKT, and NF-κB were normalized to GAPDH. Each experiment group contained at least three duplicates. Each duplicate was measured at least three times. Values are expressed as mean ± SD. Bars with different superscripts letters show marked differences ( p

    Journal: International Journal of Molecular Sciences

    Article Title: Up-Regulation of miR-130b-3p Activates the PTEN/PI3K/AKT/NF-κB Pathway to Defense against Mycoplasma gallisepticum (HS Strain) Infection of Chicken

    doi: 10.3390/ijms19082172

    Figure Lengend Snippet: miR-130b-3p activates PI3K/AKT/NF-κB pathway. DF-1 cells cultured in six-well culture dishes were transfected with miR-130b-3p or miR-130b-3p-NC. After 48 h, the expressions of PI3K ( a ), AKT ( b ), and NF-κB ( c ) were examined by RT-qPCR. Similarly, DF-1 cells transfected with miR-130b-3p-Inh or miR-130b-3p-Inh-NC were incubated for 48 h. Then, the expressions of PI3K ( d ), AKT ( e ), and NF-κB ( f ) were examined through RT-qPCR. The relative expressions of PI3K, AKT, and NF-κB were normalized to GAPDH. Each experiment group contained at least three duplicates. Each duplicate was measured at least three times. Values are expressed as mean ± SD. Bars with different superscripts letters show marked differences ( p

    Article Snippet: Subsequently, the relative expression levels of miR-130b-3p, PTEN, PI3K, AKT, and NF-κB were detected by a real-time qPCR instrument (Bio-Rad, Hercules, CA, USA) with SuperReal PreMix Plus SYBR Green (TIANGEN, Beijing, China).

    Techniques: Cell Culture, Transfection, Quantitative RT-PCR, Incubation

    miR-130b-3p was highly expressed in both MG -infected chicken embryo lungs and DF-1 cells. ( a ) The relative level of miR-130b-3p in MG -infected chicken embryo lungs. Total RNA were extracted from frozen infected chicken embryo lungs on days 5, 7, and 9 post-infection (equivalent to days 14, 16, and 18 of eggs hatching) using TRNzol Universal. Then, the level of miR-130b-3p in MG infected embryo chicken lungs was determined through RT-qPCR; ( b ) The relative level of miR-130b-3p in MG -infected DF-1 cells. Cells cultivated in 6-well culture dishes were treated with 200 μL MG (1 × 10 10 CCU/mL). After 24 h treatment, total RNA of infected cells were extracted using TRNzol Universal. The level of miR-130b-3p-infected cells was detected by RT-qPCR. The data was normalized to 5S-rRNA. Each experiment group contained at least three duplicates. Each duplicate was measured at least three times. All values are expressed as mean ± SD. Marked differences were expressed as * p

    Journal: International Journal of Molecular Sciences

    Article Title: Up-Regulation of miR-130b-3p Activates the PTEN/PI3K/AKT/NF-κB Pathway to Defense against Mycoplasma gallisepticum (HS Strain) Infection of Chicken

    doi: 10.3390/ijms19082172

    Figure Lengend Snippet: miR-130b-3p was highly expressed in both MG -infected chicken embryo lungs and DF-1 cells. ( a ) The relative level of miR-130b-3p in MG -infected chicken embryo lungs. Total RNA were extracted from frozen infected chicken embryo lungs on days 5, 7, and 9 post-infection (equivalent to days 14, 16, and 18 of eggs hatching) using TRNzol Universal. Then, the level of miR-130b-3p in MG infected embryo chicken lungs was determined through RT-qPCR; ( b ) The relative level of miR-130b-3p in MG -infected DF-1 cells. Cells cultivated in 6-well culture dishes were treated with 200 μL MG (1 × 10 10 CCU/mL). After 24 h treatment, total RNA of infected cells were extracted using TRNzol Universal. The level of miR-130b-3p-infected cells was detected by RT-qPCR. The data was normalized to 5S-rRNA. Each experiment group contained at least three duplicates. Each duplicate was measured at least three times. All values are expressed as mean ± SD. Marked differences were expressed as * p

    Article Snippet: Subsequently, the relative expression levels of miR-130b-3p, PTEN, PI3K, AKT, and NF-κB were detected by a real-time qPCR instrument (Bio-Rad, Hercules, CA, USA) with SuperReal PreMix Plus SYBR Green (TIANGEN, Beijing, China).

    Techniques: Infection, Quantitative RT-PCR

    The DNA methylation level in the 5′ flanking region of C3 and KNG1 after GCRV infection. ( a ) A schematic diagram of CpG loci in the 5′ flanking region of C3 and KNG1 genes. Four CpG loci were selected in both C3 (-293, -192, -164, and -132) and KNG1 (-215, -207, -115, and -104) genes for BSP-PCR. ( b ) Changes of DNA methylation levels after GCRV infection. Data are shown as the mean ± standard deviation of three biological duplicates ( n = 5 for each biological duplicate). Significant difference ( p

    Journal: International Journal of Molecular Sciences

    Article Title: Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection

    doi: 10.3390/ijms19041110

    Figure Lengend Snippet: The DNA methylation level in the 5′ flanking region of C3 and KNG1 after GCRV infection. ( a ) A schematic diagram of CpG loci in the 5′ flanking region of C3 and KNG1 genes. Four CpG loci were selected in both C3 (-293, -192, -164, and -132) and KNG1 (-215, -207, -115, and -104) genes for BSP-PCR. ( b ) Changes of DNA methylation levels after GCRV infection. Data are shown as the mean ± standard deviation of three biological duplicates ( n = 5 for each biological duplicate). Significant difference ( p

    Article Snippet: To investigate the gene expression levels of DNMT1 , DNMT2 , DNMT6 , DNMT7 , TET-1 , TET-2 , TET-3 , GNMT , C3 , and KNG1 , RT-qPCR was carried out using a fluorescence quantitative PCR instrument (Bio-Rad, Hercules, CA, USA).

    Techniques: DNA Methylation Assay, Infection, Polymerase Chain Reaction, Standard Deviation

    qRT-PCR analysis of the expression levels of C3 and KNG1 after GCRV infection. The relative expression levels of the genes were calculated as the ratio of gene expression level in GCRV infected group relative to that in the control group at the same time-point. All data represent the mean ± standard deviation of three replicates ( n = 5 for each biological duplicate). Significant difference ( p

    Journal: International Journal of Molecular Sciences

    Article Title: Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection

    doi: 10.3390/ijms19041110

    Figure Lengend Snippet: qRT-PCR analysis of the expression levels of C3 and KNG1 after GCRV infection. The relative expression levels of the genes were calculated as the ratio of gene expression level in GCRV infected group relative to that in the control group at the same time-point. All data represent the mean ± standard deviation of three replicates ( n = 5 for each biological duplicate). Significant difference ( p

    Article Snippet: To investigate the gene expression levels of DNMT1 , DNMT2 , DNMT6 , DNMT7 , TET-1 , TET-2 , TET-3 , GNMT , C3 , and KNG1 , RT-qPCR was carried out using a fluorescence quantitative PCR instrument (Bio-Rad, Hercules, CA, USA).

    Techniques: Quantitative RT-PCR, Expressing, Infection, Standard Deviation

    Details of the PCR setup. Detailed information of the PCR setup on the ViiA7 instrument with telomere primers ( A ; annealing at 56 °C) and human β-globin primers ( B ; annealing at 58 °C) is provided. Cycling temperatures with time, number of cycles, as well as ramping rates are clearly indicated here. A melt curve analysis is added at the end of 40 cycles of PCR to ensure the absence of primer-dimers.

    Journal: Methods and Protocols

    Article Title: An Optimised Step-by-Step Protocol for Measuring Relative Telomere Length

    doi: 10.3390/mps3020027

    Figure Lengend Snippet: Details of the PCR setup. Detailed information of the PCR setup on the ViiA7 instrument with telomere primers ( A ; annealing at 56 °C) and human β-globin primers ( B ; annealing at 58 °C) is provided. Cycling temperatures with time, number of cycles, as well as ramping rates are clearly indicated here. A melt curve analysis is added at the end of 40 cycles of PCR to ensure the absence of primer-dimers.

    Article Snippet: ViiA7 qPCR machine with fast 96-well blocks (ThermoFisher Scientific, Waltham, MA, USA; Cat. no.: 4453535).

    Techniques: Polymerase Chain Reaction

    Genes significantly deregulated in HCT116 cells with corrected PRDM2 (A-B) Top 10 PRDM2-deregulated genes identified under normal (A) and reduced (B) serum conditions. (C-D) RT-qPCR validation of PRDM2-deregulated target genes identified under normal (C) and reduced (D) serum conditions. (E-F) RT-qPCR validation of PRDM2-deregulated genes involved in EMT under normal (E) and reduced (F) serum conditions. Genes validated in at least two of the three clones are shown. Bars represent minimum and maximum relative quantity (RQ) calculated by the StepOne software (Applied Biosystems). The dotted line indicates the level of gene expression in parental HCT116 cells. Data from one experiment are presented.

    Journal: Oncotarget

    Article Title: Somatic PRDM2 c.4467delA mutations in colorectal cancers control histone methylation and tumor growth

    doi: 10.18632/oncotarget.21713

    Figure Lengend Snippet: Genes significantly deregulated in HCT116 cells with corrected PRDM2 (A-B) Top 10 PRDM2-deregulated genes identified under normal (A) and reduced (B) serum conditions. (C-D) RT-qPCR validation of PRDM2-deregulated target genes identified under normal (C) and reduced (D) serum conditions. (E-F) RT-qPCR validation of PRDM2-deregulated genes involved in EMT under normal (E) and reduced (F) serum conditions. Genes validated in at least two of the three clones are shown. Bars represent minimum and maximum relative quantity (RQ) calculated by the StepOne software (Applied Biosystems). The dotted line indicates the level of gene expression in parental HCT116 cells. Data from one experiment are presented.

    Article Snippet: RT-qPCR was performed with Maxima SYBR Green/Rox qPCR Master Mix (Thermo Scientific) in an StepOne qPCR instrument (Applied Biosystems) with primers listed in .

    Techniques: Quantitative RT-PCR, Clone Assay, Software, Expressing