qpcr assay Search Results


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  • 93
    Roche quantitative pcr analysis a lightcyler 96 quantitative polymerase chain reaction qpcr system
    Quantitative Pcr Analysis A Lightcyler 96 Quantitative Polymerase Chain Reaction Qpcr System, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 4 article reviews
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    90
    Bio-Rad quantitative polymerase chain reaction qpcr quantitative pcr
    Quantitative <t>PCR</t> analysis of singly (SS) and multiply (MS) cellular HIV-1 RNA in the absence and presence of 1 μM prostratin and/or different concentrations of PF-3758309, danusertib, AZ 628, and P276-00. A total of 1 × 10 6 24ST1NLESG cells/well were exposed to prostratin (LRA) ± kinase inhibitor for 48 h, following which the total intracellular RNA from each condition was subjected to reverse transcription-quantitative PCR <t>(RT-qPCR)</t> analysis, as described in the Methods and Materials. Multiply spliced (MS) and singly spliced (SS) HIV-1 RNA were quantified relative to cellular IPO8 expression. Data are the average of 2 independent experiments.
    Quantitative Polymerase Chain Reaction Qpcr Quantitative Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative polymerase chain reaction qpcr
    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using <t>qPCR.</t> Levels of target gene mRNA were normalized to 18S ribosomal <t>RNA</t> and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P
    Quantitative Polymerase Chain Reaction Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1694 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche qpcr quantitative polymerase chain reaction qpcr
    Quantitative PCR results of the 3 confirmed <t>CNV</t> differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female
    Qpcr Quantitative Polymerase Chain Reaction Qpcr, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative pcr qpcr
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Quantitative Pcr Qpcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 658 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative polymerase chain reaction pcr kits
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Quantitative Polymerase Chain Reaction Pcr Kits, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG fluorescence quantitative polymerase chain reaction pcr
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Fluorescence Quantitative Polymerase Chain Reaction Pcr, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eppendorf AG fluorescent quantitative polymerase chain reaction pcr
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Fluorescent Quantitative Polymerase Chain Reaction Pcr, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler quantitative polymerase chain reaction pcr
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Lightcycler Quantitative Polymerase Chain Reaction Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7900ht quantitative polymerase chain reaction pcr instrument
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    7900ht Quantitative Polymerase Chain Reaction Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fluorescence quantitative polymerase chain reaction pcr instrument
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Fluorescence Quantitative Polymerase Chain Reaction Pcr Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 17 article reviews
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    88
    TaKaRa quantitative polymerase chain reaction qpcr kit
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Quantitative Polymerase Chain Reaction Qpcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene quantitative polymerase chain reaction qpcr machine
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Quantitative Polymerase Chain Reaction Qpcr Machine, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kapa Biosystems quantitative polymerase chain reaction qpcr reagent
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Quantitative Polymerase Chain Reaction Qpcr Reagent, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore quantitative polymerase chain reaction qpcr primers
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Quantitative Polymerase Chain Reaction Qpcr Primers, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative polymerase chain reaction qpcr kits
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Quantitative Polymerase Chain Reaction Qpcr Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TIB MOLBIOL quantitative polymerase chain reacton qpcr primers
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Quantitative Polymerase Chain Reacton Qpcr Primers, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co quantitative polymerase chain reaction qpcr kit
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Quantitative Polymerase Chain Reaction Qpcr Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Bio-Rad fluorescence quantitative polymerase chain reaction pcr instrument
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Fluorescence Quantitative Polymerase Chain Reaction Pcr Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Shanghai Kehua fluorescence quantitative polymerase chain reaction fq pcr
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Fluorescence Quantitative Polymerase Chain Reaction Fq Pcr, supplied by Shanghai Kehua, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad myiq quantitative polymerase chain reaction pcr machine
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Myiq Quantitative Polymerase Chain Reaction Pcr Machine, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative real time polymerase chain reaction pcr quantitative real time polymerase chain reaction
    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative <t>PCR,</t> is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
    Quantitative Real Time Polymerase Chain Reaction Pcr Quantitative Real Time Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Quantitative PCR analysis of singly (SS) and multiply (MS) cellular HIV-1 RNA in the absence and presence of 1 μM prostratin and/or different concentrations of PF-3758309, danusertib, AZ 628, and P276-00. A total of 1 × 10 6 24ST1NLESG cells/well were exposed to prostratin (LRA) ± kinase inhibitor for 48 h, following which the total intracellular RNA from each condition was subjected to reverse transcription-quantitative PCR (RT-qPCR) analysis, as described in the Methods and Materials. Multiply spliced (MS) and singly spliced (SS) HIV-1 RNA were quantified relative to cellular IPO8 expression. Data are the average of 2 independent experiments.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Inhibitors of Signaling Pathways That Block Reversal of HIV-1 Latency

    doi: 10.1128/AAC.01744-18

    Figure Lengend Snippet: Quantitative PCR analysis of singly (SS) and multiply (MS) cellular HIV-1 RNA in the absence and presence of 1 μM prostratin and/or different concentrations of PF-3758309, danusertib, AZ 628, and P276-00. A total of 1 × 10 6 24ST1NLESG cells/well were exposed to prostratin (LRA) ± kinase inhibitor for 48 h, following which the total intracellular RNA from each condition was subjected to reverse transcription-quantitative PCR (RT-qPCR) analysis, as described in the Methods and Materials. Multiply spliced (MS) and singly spliced (SS) HIV-1 RNA were quantified relative to cellular IPO8 expression. Data are the average of 2 independent experiments.

    Article Snippet: RNA was reverse transcribed using random primers with the AffinityScript multiple temperature reverse transcriptase (Agilent, Wilmington, DE), and quantitative PCR (qPCR) was performed using a Bio-Rad CFX96 real-time PCR detection system, using 2× Lightcycler 480 probes master (Roche, Indianapolis, IN).

    Techniques: Real-time Polymerase Chain Reaction, Mass Spectrometry, Quantitative RT-PCR, Expressing

    Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Journal: Carcinogenesis

    Article Title: Flaxseed lignans enriched in secoisolariciresinol diglucoside prevent acute asbestos-induced peritoneal inflammation in mice

    doi: 10.1093/carcin/bgv174

    Figure Lengend Snippet: Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Article Snippet: Reverse transcription of RNA to cDNA was then performed on a Veriti® Thermal Cycler using the high capacity RNA to cDNA kit supplied by Applied Biosystems Quantitative polymerase chain reaction (qPCR) and performed using TaqMan® probe-based gene expression assays supplied by Applied Biosystems, Life Technologies (Carlsbad, CA).

    Techniques: Expressing, CTL Assay, Real-time Polymerase Chain Reaction

    Flaxseed lignan diet abrogates increased nitrosative and oxidative stress from exposure to asbestos fibers. Mouse cohorts (13 weeks old) were fed CTL ( n = 16) or FLC ( n = 17) diets for 7 days prior to exposure to 400 µg of crocidolite asbestos fibers. PLF was evaluated 3 days following asbestos exposure for nitrite ( A ) and MDA concentrations ( C ). PLF WBC mRNA expression of iNOS ( B ), HO-1 ( D ), Nqo1 ( E ) and Gstm1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Journal: Carcinogenesis

    Article Title: Flaxseed lignans enriched in secoisolariciresinol diglucoside prevent acute asbestos-induced peritoneal inflammation in mice

    doi: 10.1093/carcin/bgv174

    Figure Lengend Snippet: Flaxseed lignan diet abrogates increased nitrosative and oxidative stress from exposure to asbestos fibers. Mouse cohorts (13 weeks old) were fed CTL ( n = 16) or FLC ( n = 17) diets for 7 days prior to exposure to 400 µg of crocidolite asbestos fibers. PLF was evaluated 3 days following asbestos exposure for nitrite ( A ) and MDA concentrations ( C ). PLF WBC mRNA expression of iNOS ( B ), HO-1 ( D ), Nqo1 ( E ) and Gstm1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Article Snippet: Reverse transcription of RNA to cDNA was then performed on a Veriti® Thermal Cycler using the high capacity RNA to cDNA kit supplied by Applied Biosystems Quantitative polymerase chain reaction (qPCR) and performed using TaqMan® probe-based gene expression assays supplied by Applied Biosystems, Life Technologies (Carlsbad, CA).

    Techniques: CTL Assay, Multiple Displacement Amplification, Expressing, Real-time Polymerase Chain Reaction

    Flaxseed lignan diet ameliorates asbestos-induced WBC proinflammatory cytokine release and gene expression levels. PLF levels (pg/ml for IL-1ß and IL-6 and ng/ml for HMGB1) of proinflammatory cytokines IL-1ß ( A ), IL-6 ( C ) and HMGB1 ( E ) were evaluated 3 days post asbestos exposure. PLF WBC mRNA expression of IL-1ß ( B ), IL-6 ( D ) and HMGB1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Journal: Carcinogenesis

    Article Title: Flaxseed lignans enriched in secoisolariciresinol diglucoside prevent acute asbestos-induced peritoneal inflammation in mice

    doi: 10.1093/carcin/bgv174

    Figure Lengend Snippet: Flaxseed lignan diet ameliorates asbestos-induced WBC proinflammatory cytokine release and gene expression levels. PLF levels (pg/ml for IL-1ß and IL-6 and ng/ml for HMGB1) of proinflammatory cytokines IL-1ß ( A ), IL-6 ( C ) and HMGB1 ( E ) were evaluated 3 days post asbestos exposure. PLF WBC mRNA expression of IL-1ß ( B ), IL-6 ( D ) and HMGB1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

    Article Snippet: Reverse transcription of RNA to cDNA was then performed on a Veriti® Thermal Cycler using the high capacity RNA to cDNA kit supplied by Applied Biosystems Quantitative polymerase chain reaction (qPCR) and performed using TaqMan® probe-based gene expression assays supplied by Applied Biosystems, Life Technologies (Carlsbad, CA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, CTL Assay

    Quantitative PCR results of the 3 confirmed CNV differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female

    Journal: Molecular Syndromology

    Article Title: Differences in Copy Number Variation between Discordant Monozygotic Twins as a Model for Exploring Chromosomal Mosaicism in Congenital Heart Defects

    doi: 10.1159/000335284

    Figure Lengend Snippet: Quantitative PCR results of the 3 confirmed CNV differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female

    Article Snippet: To confirm the presence of CNV differences, quantitative polymerase chain reaction (qPCR) was performed on DNA from both twin members and 3 unrelated controls (2 males and 1 female) using the LightCycler® 480 Real-Time PCR system (Roche Applied Science, Belgium, ), following the manufacturer's instructions.

    Techniques: Real-time Polymerase Chain Reaction

    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative PCR, is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p

    Journal: BMC Plant Biology

    Article Title: Genome-wide Identification and Expression Analysis of the CDPK Gene Family in Grape, Vitis spp

    doi: 10.1186/s12870-015-0552-z

    Figure Lengend Snippet: Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative PCR, is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p

    Article Snippet: Quantitative PCR (qPCR) was carried out using SYBR green (Takara, Dalian, China) on an IQ5 real time PCR machine (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) under salt and temperature stress treatments. Expression was measured by reverse transcription, followed by real-time, quantitative PCR, is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps a and histogram b . Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under NaCl, 4 °C, and 42 °C treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under NaCl, 4 °C, and 42 °C treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p

    Journal: BMC Plant Biology

    Article Title: Genome-wide Identification and Expression Analysis of the CDPK Gene Family in Grape, Vitis spp

    doi: 10.1186/s12870-015-0552-z

    Figure Lengend Snippet: Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) under salt and temperature stress treatments. Expression was measured by reverse transcription, followed by real-time, quantitative PCR, is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps a and histogram b . Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under NaCl, 4 °C, and 42 °C treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under NaCl, 4 °C, and 42 °C treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p

    Article Snippet: Quantitative PCR (qPCR) was carried out using SYBR green (Takara, Dalian, China) on an IQ5 real time PCR machine (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) during powdery mildew infection. Expression was measured by reverse transcription, followed by real-time, quantitative PCR, is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps (A) and histogram (B). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. (A) Expression profile of VpCDPK gene family under powdery mildew infection. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. (B) Detailed expression levels of four VpCDPK genes that significantly up-regulated during powdery mildew infection. The data were showed as mean value ± SD. * and ** represent statistically significant (p

    Journal: BMC Plant Biology

    Article Title: Genome-wide Identification and Expression Analysis of the CDPK Gene Family in Grape, Vitis spp

    doi: 10.1186/s12870-015-0552-z

    Figure Lengend Snippet: Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) during powdery mildew infection. Expression was measured by reverse transcription, followed by real-time, quantitative PCR, is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps (A) and histogram (B). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. (A) Expression profile of VpCDPK gene family under powdery mildew infection. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. (B) Detailed expression levels of four VpCDPK genes that significantly up-regulated during powdery mildew infection. The data were showed as mean value ± SD. * and ** represent statistically significant (p

    Article Snippet: Quantitative PCR (qPCR) was carried out using SYBR green (Takara, Dalian, China) on an IQ5 real time PCR machine (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.

    Techniques: Expressing, Infection, Real-time Polymerase Chain Reaction