Journal: Carcinogenesis

Article Title: Flaxseed lignans enriched in secoisolariciresinol diglucoside prevent acute asbestos-induced peritoneal inflammation in mice

doi: 10.1093/carcin/bgv174

Figure Lengend Snippet: Flaxseed lignan diet blunts proinflammatory and profibrogenic cytokine levels induced by asbestos exposure and alters cytokine and cytokine receptor gene expression. PLF levels (pg/ml) of proinflammatory TNFα ( A ) and profibrogenic free, active TGFß1 ( C ) were evaluated 3 days post asbestos exposure from mouse cohorts fed CTL or FLC diets. PLF WBC mRNA expression of cytokines, TNFα ( B ) and TGFß1 ( D ) and cytokine receptors, TNFαR1 ( E ) and TGFßR1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

Article Snippet: Reverse transcription of RNA to cDNA was then performed on a Veriti® Thermal Cycler using the high capacity RNA to cDNA kit supplied by Applied Biosystems Quantitative polymerase chain reaction (qPCR) and performed using TaqMan® probe-based gene expression assays supplied by Applied Biosystems, Life Technologies (Carlsbad, CA).

Techniques: Expressing, CTL Assay, Real-time Polymerase Chain Reaction

Journal: Carcinogenesis

Article Title: Flaxseed lignans enriched in secoisolariciresinol diglucoside prevent acute asbestos-induced peritoneal inflammation in mice

doi: 10.1093/carcin/bgv174

Figure Lengend Snippet: Flaxseed lignan diet abrogates increased nitrosative and oxidative stress from exposure to asbestos fibers. Mouse cohorts (13 weeks old) were fed CTL ( n = 16) or FLC ( n = 17) diets for 7 days prior to exposure to 400 µg of crocidolite asbestos fibers. PLF was evaluated 3 days following asbestos exposure for nitrite ( A ) and MDA concentrations ( C ). PLF WBC mRNA expression of iNOS ( B ), HO-1 ( D ), Nqo1 ( E ) and Gstm1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

Article Snippet: Reverse transcription of RNA to cDNA was then performed on a Veriti® Thermal Cycler using the high capacity RNA to cDNA kit supplied by Applied Biosystems Quantitative polymerase chain reaction (qPCR) and performed using TaqMan® probe-based gene expression assays supplied by Applied Biosystems, Life Technologies (Carlsbad, CA).

Techniques: CTL Assay, Multiple Displacement Amplification, Expressing, Real-time Polymerase Chain Reaction

Journal: Carcinogenesis

Article Title: Flaxseed lignans enriched in secoisolariciresinol diglucoside prevent acute asbestos-induced peritoneal inflammation in mice

doi: 10.1093/carcin/bgv174

Figure Lengend Snippet: Flaxseed lignan diet ameliorates asbestos-induced WBC proinflammatory cytokine release and gene expression levels. PLF levels (pg/ml for IL-1ß and IL-6 and ng/ml for HMGB1) of proinflammatory cytokines IL-1ß ( A ), IL-6 ( C ) and HMGB1 ( E ) were evaluated 3 days post asbestos exposure. PLF WBC mRNA expression of IL-1ß ( B ), IL-6 ( D ) and HMGB1 ( F ) was determined using qPCR. Levels of target gene mRNA were normalized to 18S ribosomal RNA and values are expressed as fold change from CTL. # indicates a statistically significant difference (#### P

Article Snippet: Reverse transcription of RNA to cDNA was then performed on a Veriti® Thermal Cycler using the high capacity RNA to cDNA kit supplied by Applied Biosystems Quantitative polymerase chain reaction (qPCR) and performed using TaqMan® probe-based gene expression assays supplied by Applied Biosystems, Life Technologies (Carlsbad, CA).

Techniques: Expressing, Real-time Polymerase Chain Reaction, CTL Assay

Journal: Molecular Syndromology

Article Title: Differences in Copy Number Variation between Discordant Monozygotic Twins as a Model for Exploring Chromosomal Mosaicism in Congenital Heart Defects

doi: 10.1159/000335284

Figure Lengend Snippet: Quantitative PCR results of the 3 confirmed CNV differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female

Article Snippet: To confirm the presence of CNV differences, quantitative polymerase chain reaction (qPCR) was performed on DNA from both twin members and 3 unrelated controls (2 males and 1 female) using the LightCycler® 480 Real-Time PCR system (Roche Applied Science, Belgium, ), following the manufacturer's instructions.

Techniques: Real-time Polymerase Chain Reaction

Journal: BMC Plant Biology

Article Title: Genome-wide Identification and Expression Analysis of the CDPK Gene Family in Grape, Vitis spp

doi: 10.1186/s12870-015-0552-z

Figure Lengend Snippet: Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative PCR, is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p

Article Snippet: Quantitative PCR (qPCR) was carried out using SYBR green (Takara, Dalian, China) on an IQ5 real time PCR machine (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: BMC Plant Biology

Article Title: Genome-wide Identification and Expression Analysis of the CDPK Gene Family in Grape, Vitis spp

doi: 10.1186/s12870-015-0552-z

Figure Lengend Snippet: Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) under salt and temperature stress treatments. Expression was measured by reverse transcription, followed by real-time, quantitative PCR, is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps a and histogram b . Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under NaCl, 4 °C, and 42 °C treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under NaCl, 4 °C, and 42 °C treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p

Article Snippet: Quantitative PCR (qPCR) was carried out using SYBR green (Takara, Dalian, China) on an IQ5 real time PCR machine (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: BMC Plant Biology

Article Title: Genome-wide Identification and Expression Analysis of the CDPK Gene Family in Grape, Vitis spp

doi: 10.1186/s12870-015-0552-z

Figure Lengend Snippet: Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) during powdery mildew infection. Expression was measured by reverse transcription, followed by real-time, quantitative PCR, is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps (A) and histogram (B). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. (A) Expression profile of VpCDPK gene family under powdery mildew infection. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. (B) Detailed expression levels of four VpCDPK genes that significantly up-regulated during powdery mildew infection. The data were showed as mean value ± SD. * and ** represent statistically significant (p

Article Snippet: Quantitative PCR (qPCR) was carried out using SYBR green (Takara, Dalian, China) on an IQ5 real time PCR machine (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.

Techniques: Expressing, Infection, Real-time Polymerase Chain Reaction