Journal: BMC Plant Biology
Article Title: Genome-wide Identification and Expression Analysis of the CDPK Gene Family in Grape, Vitis spp
Figure Lengend Snippet: Expression of CDPK genes in Chinese wild grape ( Vitis pseudoreticulata ) in response to treatment with plant hormones. Expression was measured by reverse transcription, followed by real-time, quantitative PCR, is indicated as fold-change of experimental treatments relative to control samples, and is visualized as heatmaps ( a ) and histogram ( b ). Grape Actin1 (GenBank Accession number AY680701) was used as an internal control. The experiments were repeated three times and gave consistent results. Mean values and SDs were obtained from three biological and three technical replicates. a Expression profile of VpCDPK gene family under ABA, SA, MeJA, and ethylene (ETH) treatments. The color scale represents log2 expression values, with red indicating increased transcript abundance and green indicating decreased transcript abundance. b Detailed expression levels of four VpCDPK genes showed unusual expression patterns under ABA, SA, MeJA, and ethylene (ETH) treatments. The data were showed as mean value ± SD. * and ** represent statistically significant (p
Article Snippet: Quantitative PCR (qPCR) was carried out using SYBR green (Takara, Dalian, China) on an IQ5 real time PCR machine (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions.
Techniques: Expressing, Real-time Polymerase Chain Reaction