Journal: Experimental and Therapeutic Medicine
Article Title: Induction of reprogramming of human amniotic epithelial cells into iPS cells by overexpression of Yap, Oct4, and Sox2 through the activation of the Hippo-Yap pathway
Figure Lengend Snippet: Overexpression the three factors, OSY, induced HuAECs to express high levels of ESC markers. (A) The process of induction of HuAEC reprogramming into iPS cells by OSY. (B) Cell morphology of HuAECs; scale, 30 µm. (C) OSY-iPS cells had clone-like morphology; scale, 30 µm. (D) Alkaline phosphatase staining identification of OSY-iPS cells was positive; scale, 30 µm. (E) Semi-quantitative PCR results indicated that OSY-iPS cells expressed high levels of ESC markers (Oct4, Sox2, Nanog, Rex1, and Ssea3/4) and Yap. (F) Immunofluorescence staining results suggested that OSY-iPS cells expressed high levels of Oct4 and SSEA3/4 proteins; scale, 30 µm. (G) Western blot results showed that OSY-iPS cells expressed high levels of Oct4, Sox2, and Yap proteins.
Article Snippet: Quantitative polymerase chain reaction (qPCR) was performed using a RealPlex4 real-time PCR detection system (Eppendorf Co., Ltd., Hamburg, Germany).
Techniques: Over Expression, Staining, Real-time Polymerase Chain Reaction, Immunofluorescence, Western Blot