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    For use with the GeneRead Library Quant System Kit contents Two 2 tubes each containing 1 35 ml of 2x solution sufficient for 100 standard 25 l reactions Benefits Specific
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    384 well qPCR plate PP Full Skirt 5 bags of 10 plate
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    99
    Roche quantitative polymerase chain reaction qpcr
    Quantitative PCR results of the 3 confirmed <t>CNV</t> differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female
    Quantitative Polymerase Chain Reaction Qpcr, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher quantitative polymerase chain reaction qpcr
    Quantitative PCR results of the 3 confirmed <t>CNV</t> differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female
    Quantitative Polymerase Chain Reaction Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1509 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative polymerase chain reaction qpcr
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Quantitative Polymerase Chain Reaction Qpcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co quantitative polymerase chain reaction qpcr kit
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Quantitative Polymerase Chain Reaction Qpcr Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene quantitative polymerase chain reaction qpcr machine
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Quantitative Polymerase Chain Reaction Qpcr Machine, supplied by Stratagene, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa quantitative polymerase chain reaction qpcr kit
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Quantitative Polymerase Chain Reaction Qpcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative polymerase chain reaction qpcr kits
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Quantitative Polymerase Chain Reaction Qpcr Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kapa Biosystems quantitative polymerase chain reaction qpcr reagent
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Quantitative Polymerase Chain Reaction Qpcr Reagent, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore quantitative polymerase chain reaction qpcr primers
    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) <t>mRNA</t> expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P
    Quantitative Polymerase Chain Reaction Qpcr Primers, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad quantitative polymerase chain reaction qpcr quantitative pcr
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Quantitative Polymerase Chain Reaction Qpcr Quantitative Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene mx3005p quantitative polymerase chain reaction qpcr system
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Mx3005p Quantitative Polymerase Chain Reaction Qpcr System, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Capital Biosciences evagreen quantitative polymerase chain reaction qpcr kits
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Evagreen Quantitative Polymerase Chain Reaction Qpcr Kits, supplied by Capital Biosciences, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Eppendorf AG quantitative polymerase chain reaction qpcr detection system
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Quantitative Polymerase Chain Reaction Qpcr Detection System, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 82/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative polymerase chain reaction qpcr reagents
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Quantitative Polymerase Chain Reaction Qpcr Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantitative polymerase chain reaction qpcr trizol reagent
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Quantitative Polymerase Chain Reaction Qpcr Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies mx3005p quantitative polymerase chain reaction qpcr system
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Mx3005p Quantitative Polymerase Chain Reaction Qpcr System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore quantitative polymerase chain reaction qpcr trizol reagent
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Quantitative Polymerase Chain Reaction Qpcr Trizol Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 7900ht quantitative polymerase chain reaction qpcr machine
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    7900ht Quantitative Polymerase Chain Reaction Qpcr Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher transcriptase quantitative polymerase chain reaction qpcr
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Transcriptase Quantitative Polymerase Chain Reaction Qpcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche quantitative pcr analysis a lightcyler 96 quantitative polymerase chain reaction qpcr system
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Quantitative Pcr Analysis A Lightcyler 96 Quantitative Polymerase Chain Reaction Qpcr System, supplied by Roche, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kapa Biosystems quantitative polymerase chain reaction quantitative polymerase chain reaction qpcr
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Quantitative Polymerase Chain Reaction Quantitative Polymerase Chain Reaction Qpcr, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr quantitative polymerase chain reaction
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Sybr Quantitative Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Stratagene quantitative polymerase chain reaction apparatus
    Fold decrements of template DNA of the <t>β-globin</t> and rhodopsin genes after MNase digestion of chromatin samples quantified by <t>qPCR.</t> Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .
    Quantitative Polymerase Chain Reaction Apparatus, supplied by Stratagene, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Protein Simple Inc transcription quantitative polymerase chain reaction
    (A) Reverse <t>transcription-quantitative</t> <t>polymerase</t> <t>chain</t> <t>reaction</t> was used to detect the TRAP1 mRNA expression levels in Hep3b2.1–7, HepG2, HepG2.2.15 and Sk-hep1 cells. (B) The protein levels of TRAP1 in the 4 liver cancer cell lines were determined by western blot analysis. β-actin was used as an internal control. (C) WES™ automated capillary-based size sorting system was used to measure the protein level of TRAP1 and β-actin in 4 liver cancer cell lines. (D) The protein levels of TRAP1 were examined in 4 liver cancer cells treated with 0, 5, 10 and 15 µmol/l rapamycin by western blot analysis. β-actin was used as an internal control. *P
    Transcription Quantitative Polymerase Chain Reaction, supplied by Protein Simple Inc, used in various techniques. Bioz Stars score: 84/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher transcription quantitative polymerase chain reaction
    (A) Reverse <t>transcription-quantitative</t> <t>polymerase</t> <t>chain</t> <t>reaction</t> was used to detect the TRAP1 mRNA expression levels in Hep3b2.1–7, HepG2, HepG2.2.15 and Sk-hep1 cells. (B) The protein levels of TRAP1 in the 4 liver cancer cell lines were determined by western blot analysis. β-actin was used as an internal control. (C) WES™ automated capillary-based size sorting system was used to measure the protein level of TRAP1 and β-actin in 4 liver cancer cell lines. (D) The protein levels of TRAP1 were examined in 4 liver cancer cells treated with 0, 5, 10 and 15 µmol/l rapamycin by western blot analysis. β-actin was used as an internal control. *P
    Transcription Quantitative Polymerase Chain Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kapa Biosystems kapa quantitative polymerase chain reaction
    (A) Reverse <t>transcription-quantitative</t> <t>polymerase</t> <t>chain</t> <t>reaction</t> was used to detect the TRAP1 mRNA expression levels in Hep3b2.1–7, HepG2, HepG2.2.15 and Sk-hep1 cells. (B) The protein levels of TRAP1 in the 4 liver cancer cell lines were determined by western blot analysis. β-actin was used as an internal control. (C) WES™ automated capillary-based size sorting system was used to measure the protein level of TRAP1 and β-actin in 4 liver cancer cell lines. (D) The protein levels of TRAP1 were examined in 4 liver cancer cells treated with 0, 5, 10 and 15 µmol/l rapamycin by western blot analysis. β-actin was used as an internal control. *P
    Kapa Quantitative Polymerase Chain Reaction, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Thermo Fisher quantitative polymerase chain reaction trizol
    (A) Reverse <t>transcription-quantitative</t> <t>polymerase</t> <t>chain</t> <t>reaction</t> was used to detect the TRAP1 mRNA expression levels in Hep3b2.1–7, HepG2, HepG2.2.15 and Sk-hep1 cells. (B) The protein levels of TRAP1 in the 4 liver cancer cell lines were determined by western blot analysis. β-actin was used as an internal control. (C) WES™ automated capillary-based size sorting system was used to measure the protein level of TRAP1 and β-actin in 4 liver cancer cell lines. (D) The protein levels of TRAP1 were examined in 4 liver cancer cells treated with 0, 5, 10 and 15 µmol/l rapamycin by western blot analysis. β-actin was used as an internal control. *P
    Quantitative Polymerase Chain Reaction Trizol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Eppendorf AG quantitative polymerase chain reaction qpcr
    Overexpression the three factors, OSY, induced HuAECs to express high levels of ESC markers. (A) The process of induction of HuAEC reprogramming into iPS cells by OSY. (B) Cell morphology of HuAECs; scale, 30 µm. (C) OSY-iPS cells had clone-like morphology; scale, 30 µm. (D) Alkaline phosphatase staining identification of OSY-iPS cells was positive; scale, 30 µm. (E) Semi-quantitative <t>PCR</t> results indicated that OSY-iPS cells expressed high levels of ESC markers (Oct4, Sox2, Nanog, Rex1, and Ssea3/4) and Yap. (F) Immunofluorescence staining results suggested that OSY-iPS cells expressed high levels of Oct4 and SSEA3/4 proteins; scale, 30 µm. (G) Western blot results showed that OSY-iPS cells expressed high levels of Oct4, Sox2, and Yap proteins.
    Quantitative Polymerase Chain Reaction Qpcr, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 92/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Quanta Biosciences quantitative polymerase chain reaction qpcr
    Overexpression the three factors, OSY, induced HuAECs to express high levels of ESC markers. (A) The process of induction of HuAEC reprogramming into iPS cells by OSY. (B) Cell morphology of HuAECs; scale, 30 µm. (C) OSY-iPS cells had clone-like morphology; scale, 30 µm. (D) Alkaline phosphatase staining identification of OSY-iPS cells was positive; scale, 30 µm. (E) Semi-quantitative <t>PCR</t> results indicated that OSY-iPS cells expressed high levels of ESC markers (Oct4, Sox2, Nanog, Rex1, and Ssea3/4) and Yap. (F) Immunofluorescence staining results suggested that OSY-iPS cells expressed high levels of Oct4 and SSEA3/4 proteins; scale, 30 µm. (G) Western blot results showed that OSY-iPS cells expressed high levels of Oct4, Sox2, and Yap proteins.
    Quantitative Polymerase Chain Reaction Qpcr, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative polymerase chain reaction qpcr/product/Quanta Biosciences
    Average 92 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    quantitative polymerase chain reaction qpcr - by Bioz Stars, 2020-02
    92/100 stars
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    Stratagene quantitative polymerase chain reaction qpcr
    Figure 2. IL-15 expression in malignant T cells. ( A ) Non-malignant (MySi) and malignant (PB2B, MF2000) T cells were cultured for 24 h. RNA was purified from the cells and reversely transcribed to <t>cDNA</t> and analyzed by quantitative PCR to determine
    Quantitative Polymerase Chain Reaction Qpcr, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative polymerase chain reaction qpcr/product/Stratagene
    Average 92 stars, based on 102 article reviews
    Price from $9.99 to $1999.99
    quantitative polymerase chain reaction qpcr - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

    Image Search Results


    Quantitative PCR results of the 3 confirmed CNV differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female

    Journal: Molecular Syndromology

    Article Title: Differences in Copy Number Variation between Discordant Monozygotic Twins as a Model for Exploring Chromosomal Mosaicism in Congenital Heart Defects

    doi: 10.1159/000335284

    Figure Lengend Snippet: Quantitative PCR results of the 3 confirmed CNV differences between the members of twin pair 6. Quantitative PCR results are shown for the affected and unaffected (UA) member of twin pair 6, as well as for 3 unrelated normal controls (2 males (M), 1 female

    Article Snippet: To confirm the presence of CNV differences, quantitative polymerase chain reaction (qPCR) was performed on DNA from both twin members and 3 unrelated controls (2 males and 1 female) using the LightCycler® 480 Real-Time PCR system (Roche Applied Science, Belgium, ), following the manufacturer's instructions.

    Techniques: Real-time Polymerase Chain Reaction

    ( A ) qPCR data of Bacterial and Archaeal 16S copy numbers (per g biomass) on the right y-axis from biomass samples (x-axis) throughout the trial corresponding to the ratio of bacteria to archaea (expressed as a percentage) on the left y-axis. ( B ) qPCR data of Bacterial and Archaeal 16S rRNA transcripts copy numbers (per g biomass) on the right y-axis from biomass samples (x-axis) throughout the trial corresponding to the ratio of bacteria to archaea (expressed as a percentage) on the left y-axis.

    Journal: FEMS Microbiology Ecology

    Article Title: Cold adaptation and replicable microbial community development during long-term low-temperature anaerobic digestion treatment of synthetic sewage

    doi: 10.1093/femsec/fiy095

    Figure Lengend Snippet: ( A ) qPCR data of Bacterial and Archaeal 16S copy numbers (per g biomass) on the right y-axis from biomass samples (x-axis) throughout the trial corresponding to the ratio of bacteria to archaea (expressed as a percentage) on the left y-axis. ( B ) qPCR data of Bacterial and Archaeal 16S rRNA transcripts copy numbers (per g biomass) on the right y-axis from biomass samples (x-axis) throughout the trial corresponding to the ratio of bacteria to archaea (expressed as a percentage) on the left y-axis.

    Article Snippet: Quantitative-polymerase chain reaction Quantitative polymerase chain reaction (qPCR) was carried out for Archaeal and Bacterial domains using DNA and cDNA generated from granular biomass sampled from R1 and R2 and the fixed-film filter as described above. qPCR was performed using a LightCycler 480 (Roche, Manheim, Germany).

    Techniques: Real-time Polymerase Chain Reaction

    (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) mRNA expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P

    Journal: Molecular Medicine Reports

    Article Title: Potential application of injectable chitosan hydrogel treated with siRNA in chronic rhinosinusitis therapy

    doi: 10.3892/mmr.2015.4237

    Figure Lengend Snippet: (A) Visualization of the uptake of siRNA using fluorescence microscopy. (B) mRNA expression of VEGF, determined using quantitative polymerase chain reaction analysis. (C) Associated VEGF protein level, determined by enzyme-linked immunosorbent assays. * P

    Article Snippet: The mRNA expression of target VEGF was analyzed using quantitative polymerase chain reaction (qPCR) with an SYBR Premix ExTM Taq II kit (Takara Bio, Inc.).

    Techniques: Fluorescence, Microscopy, Expressing, Real-time Polymerase Chain Reaction

    Expression levels of cullin 3 protein and mRNA and proteins of the WNK–OSR1/SPAK–NCC phosphorylation signaling cascade in the kidneys of Cullin 3 G(−1)A/+ knock-in mice. (A) Immunoblots of proteins of the WNK–OSR1/SPAK–NCC signaling cascade in the kidneys of wild-type (WT) and cullin 3 ( Cul3 ) G(−1)A/+ heterozygous knock-in mice. (B) Densitometry analysis. Values are expressed as a ratio of the average signal in WT mice. Cul3 expression levels in Cul3 G(−1)A/+ heterozygous knock-in mice were approximately half that of WT mice. There were no significant differences in proteins expression levels of the WNK–OSR1/SPAK–NCC phosphorylation signaling cascade between Cul3 G(−1)A/+ heterozygous knock-in and WT mice. (C) Quantitative polymerase chain reaction (PCR) analysis of Cul3 mRNA levels. SYBR Green quantitative PCR was used to quantify mRNA levels in the kidneys of WT mice ( n =7) and Cul3 G(−1)A/+ mice ( n =7). WT: wild type. G(−1)A: Cul3 G(−1)A/+ . * P

    Journal: Biology Open

    Article Title: Generation and analysis of knock-in mice carrying pseudohypoaldosteronism type II-causing mutations in the cullin 3 gene

    doi: 10.1242/bio.013276

    Figure Lengend Snippet: Expression levels of cullin 3 protein and mRNA and proteins of the WNK–OSR1/SPAK–NCC phosphorylation signaling cascade in the kidneys of Cullin 3 G(−1)A/+ knock-in mice. (A) Immunoblots of proteins of the WNK–OSR1/SPAK–NCC signaling cascade in the kidneys of wild-type (WT) and cullin 3 ( Cul3 ) G(−1)A/+ heterozygous knock-in mice. (B) Densitometry analysis. Values are expressed as a ratio of the average signal in WT mice. Cul3 expression levels in Cul3 G(−1)A/+ heterozygous knock-in mice were approximately half that of WT mice. There were no significant differences in proteins expression levels of the WNK–OSR1/SPAK–NCC phosphorylation signaling cascade between Cul3 G(−1)A/+ heterozygous knock-in and WT mice. (C) Quantitative polymerase chain reaction (PCR) analysis of Cul3 mRNA levels. SYBR Green quantitative PCR was used to quantify mRNA levels in the kidneys of WT mice ( n =7) and Cul3 G(−1)A/+ mice ( n =7). WT: wild type. G(−1)A: Cul3 G(−1)A/+ . * P

    Article Snippet: Quantitative polymerase chain reaction Quantitative PCR was performed using SYBR Premix Ex Taq™ II (Takara Bio Inc.) and primers specific for Cul3 , using the forward primer F4 and the reverse primer R5 (5′-AGCCCTGGATATAGTCAACAGG-3′).

    Techniques: Expressing, Knock-In, Mouse Assay, Western Blot, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, SYBR Green Assay

    Expression levels of cullin 3 protein and mRNA and proteins of the WNK-OSR1/SPAK-NCC phosphorylation signaling cascade in the kidneys of cullin 3 T(−6)G/T(−6)G knock-in mice. (A) Immunoblots of proteins of the WNK–OSR1/SPAK–NCC signaling cascade in the kidneys of wild-type (WT) and cullin 3 ( Cul3 ) T( − 6)G/T( − 6)G homozygous knock-in mice. (B) Densitometry analysis. Values are expressed as the ratio of the average signal in WT mice. The level of Cul3 protein expression in Cul3 T( − 6)G/T( − 6)G mice was three-quarters that of WT mice. There were no significant differences in proteins expression levels of the WNK–OSR1/SPAK–NCC phosphorylation signaling cascade between Cul3 T( − 6)G/T( − 6)G and WT mice. (C) Quantitative polymerase chain reaction (PCR) analysis of Cul3 mRNA levels. SYBR Green quantitative PCR was used to quantify mRNA levels in the kidneys of WT mice ( n =7) and Cul3 T( − 6)G/T( − 6)G mice ( n =9). WT: wild-type mice. T(−6)G: Cul3 T( − 6)G/T( − 6)G mice. * P

    Journal: Biology Open

    Article Title: Generation and analysis of knock-in mice carrying pseudohypoaldosteronism type II-causing mutations in the cullin 3 gene

    doi: 10.1242/bio.013276

    Figure Lengend Snippet: Expression levels of cullin 3 protein and mRNA and proteins of the WNK-OSR1/SPAK-NCC phosphorylation signaling cascade in the kidneys of cullin 3 T(−6)G/T(−6)G knock-in mice. (A) Immunoblots of proteins of the WNK–OSR1/SPAK–NCC signaling cascade in the kidneys of wild-type (WT) and cullin 3 ( Cul3 ) T( − 6)G/T( − 6)G homozygous knock-in mice. (B) Densitometry analysis. Values are expressed as the ratio of the average signal in WT mice. The level of Cul3 protein expression in Cul3 T( − 6)G/T( − 6)G mice was three-quarters that of WT mice. There were no significant differences in proteins expression levels of the WNK–OSR1/SPAK–NCC phosphorylation signaling cascade between Cul3 T( − 6)G/T( − 6)G and WT mice. (C) Quantitative polymerase chain reaction (PCR) analysis of Cul3 mRNA levels. SYBR Green quantitative PCR was used to quantify mRNA levels in the kidneys of WT mice ( n =7) and Cul3 T( − 6)G/T( − 6)G mice ( n =9). WT: wild-type mice. T(−6)G: Cul3 T( − 6)G/T( − 6)G mice. * P

    Article Snippet: Quantitative polymerase chain reaction Quantitative PCR was performed using SYBR Premix Ex Taq™ II (Takara Bio Inc.) and primers specific for Cul3 , using the forward primer F4 and the reverse primer R5 (5′-AGCCCTGGATATAGTCAACAGG-3′).

    Techniques: Expressing, Knock-In, Mouse Assay, Western Blot, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, SYBR Green Assay

    Fold decrements of template DNA of the β-globin and rhodopsin genes after MNase digestion of chromatin samples quantified by qPCR. Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .

    Journal: Nucleic Acids Research

    Article Title: Linker histone partial phosphorylation: effects on secondary structure and chromatin condensation

    doi: 10.1093/nar/gkv304

    Figure Lengend Snippet: Fold decrements of template DNA of the β-globin and rhodopsin genes after MNase digestion of chromatin samples quantified by qPCR. Dark gray, phosphorylated samples. Light gray, unphosphorylated samples. ( A, C ) Results for the β-globin gene. ( B, D ) Results for the rhodopsin gene. Fold decrements were calculated as 2 ΔCt , using the Ct value of the initial undigested chromatin sample as reference for each gene. Error bars correspond to the interval 2 ΔCt±SD .

    Article Snippet: Purified DNA was used as a template for the amplification of β-globin and rhodopsin genes by Quantitative Polymerase Chain Reaction (qPCR), following MIQE guidelines, in a C1000TM thermal cycler (BioRad).

    Techniques: Real-time Polymerase Chain Reaction

    (A) Reverse transcription-quantitative polymerase chain reaction was used to detect the TRAP1 mRNA expression levels in Hep3b2.1–7, HepG2, HepG2.2.15 and Sk-hep1 cells. (B) The protein levels of TRAP1 in the 4 liver cancer cell lines were determined by western blot analysis. β-actin was used as an internal control. (C) WES™ automated capillary-based size sorting system was used to measure the protein level of TRAP1 and β-actin in 4 liver cancer cell lines. (D) The protein levels of TRAP1 were examined in 4 liver cancer cells treated with 0, 5, 10 and 15 µmol/l rapamycin by western blot analysis. β-actin was used as an internal control. *P

    Journal: Oncology Letters

    Article Title: Effect on the liver cancer cell invasion ability by studying the associations between autophagy and TRAP1 expression

    doi: 10.3892/ol.2018.8774

    Figure Lengend Snippet: (A) Reverse transcription-quantitative polymerase chain reaction was used to detect the TRAP1 mRNA expression levels in Hep3b2.1–7, HepG2, HepG2.2.15 and Sk-hep1 cells. (B) The protein levels of TRAP1 in the 4 liver cancer cell lines were determined by western blot analysis. β-actin was used as an internal control. (C) WES™ automated capillary-based size sorting system was used to measure the protein level of TRAP1 and β-actin in 4 liver cancer cell lines. (D) The protein levels of TRAP1 were examined in 4 liver cancer cells treated with 0, 5, 10 and 15 µmol/l rapamycin by western blot analysis. β-actin was used as an internal control. *P

    Article Snippet: The mRNA and protein expression levels of tumor necrosis factor receptor-associated protein-1 (TRAP-1), Beclin1 and LC3-II/LC3-I were measured by reverse transcription-quantitative polymerase chain reaction, Protein Simple Western and western blot analysis.

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot

    Overexpression the three factors, OSY, induced HuAECs to express high levels of ESC markers. (A) The process of induction of HuAEC reprogramming into iPS cells by OSY. (B) Cell morphology of HuAECs; scale, 30 µm. (C) OSY-iPS cells had clone-like morphology; scale, 30 µm. (D) Alkaline phosphatase staining identification of OSY-iPS cells was positive; scale, 30 µm. (E) Semi-quantitative PCR results indicated that OSY-iPS cells expressed high levels of ESC markers (Oct4, Sox2, Nanog, Rex1, and Ssea3/4) and Yap. (F) Immunofluorescence staining results suggested that OSY-iPS cells expressed high levels of Oct4 and SSEA3/4 proteins; scale, 30 µm. (G) Western blot results showed that OSY-iPS cells expressed high levels of Oct4, Sox2, and Yap proteins.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Induction of reprogramming of human amniotic epithelial cells into iPS cells by overexpression of Yap, Oct4, and Sox2 through the activation of the Hippo-Yap pathway

    doi: 10.3892/etm.2017.4512

    Figure Lengend Snippet: Overexpression the three factors, OSY, induced HuAECs to express high levels of ESC markers. (A) The process of induction of HuAEC reprogramming into iPS cells by OSY. (B) Cell morphology of HuAECs; scale, 30 µm. (C) OSY-iPS cells had clone-like morphology; scale, 30 µm. (D) Alkaline phosphatase staining identification of OSY-iPS cells was positive; scale, 30 µm. (E) Semi-quantitative PCR results indicated that OSY-iPS cells expressed high levels of ESC markers (Oct4, Sox2, Nanog, Rex1, and Ssea3/4) and Yap. (F) Immunofluorescence staining results suggested that OSY-iPS cells expressed high levels of Oct4 and SSEA3/4 proteins; scale, 30 µm. (G) Western blot results showed that OSY-iPS cells expressed high levels of Oct4, Sox2, and Yap proteins.

    Article Snippet: Quantitative polymerase chain reaction (qPCR) was performed using a RealPlex4 real-time PCR detection system (Eppendorf Co., Ltd., Hamburg, Germany).

    Techniques: Over Expression, Staining, Real-time Polymerase Chain Reaction, Immunofluorescence, Western Blot

    Figure 2. IL-15 expression in malignant T cells. ( A ) Non-malignant (MySi) and malignant (PB2B, MF2000) T cells were cultured for 24 h. RNA was purified from the cells and reversely transcribed to cDNA and analyzed by quantitative PCR to determine

    Journal: Cell Cycle

    Article Title: IL-15 and IL-17F are differentially regulated and expressed in mycosis fungoides (MF)

    doi: 10.4161/cc.28256

    Figure Lengend Snippet: Figure 2. IL-15 expression in malignant T cells. ( A ) Non-malignant (MySi) and malignant (PB2B, MF2000) T cells were cultured for 24 h. RNA was purified from the cells and reversely transcribed to cDNA and analyzed by quantitative PCR to determine

    Article Snippet: Total cellular mRNA was purified and reverse transcribed into cDNA (cDNA) as described previously., Quantitative polymerase chain reaction (QPCR) was subsequently performed using the Brilliant II SYBR Green quantitative PCR kit from Stratagene in accordance with the manufacturer’s instructions and the samples analyzed on a Mx3005P (Stratagene).

    Techniques: Expressing, Cell Culture, Purification, Real-time Polymerase Chain Reaction