Journal: Applied and Environmental Microbiology
Article Title: Construction of DNA-Shuffled and Incrementally Truncated Libraries by a Mutagenic and Unidirectional Reassembly Method: Changing from a Substrate Specificity of Phospholipase to That of Lipase
Figure Lengend Snippet: Agarose gel analysis of DNA fragments generated during the MURA process. The Serratia sp. phospholipase gene (0.96 kb) was prepared by PCR (lane 1). The template DNA was digested with DNase I and the digests were purified (lane 2) and subjected to the MURA PCR as described in Materials and Methods; the resultant DNA fragments reassembled with a unidirectional primer (lane 3) were purified by using a Qiaquick purification kit. Lane M, 100-bp ladder DNA size marker.
Article Snippet: After the DNase I digests were purified with a Qiaquick nucleotide removal kit (Qiagen), it was confirmed on a 2.5% Metaphore agarose gel (FMC, Rockland, Minn.) or a 2% agarose gel, the size of which was about 100 bp.
Techniques: Agarose Gel Electrophoresis, Generated, Polymerase Chain Reaction, Purification, Marker