q5 polymerase Search Results


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  • 99
    New England Biolabs q5 polymerase
    DiPaC of the sod BGC into pET28b-ptetO:: sod_gfp v2. a PCR amplification of the 4.6 kb sodA – D fragment with <t>Q5</t> polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO:: gfp v2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO:: sod_gfp v2 plasmid and e control restriction digest of two clones with Nde I or Eco RV in comparison to the predicted (Snapgene) pattern
    Q5 Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase/product/New England Biolabs
    Average 99 stars, based on 3235 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase - by Bioz Stars, 2020-07
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    90
    TaKaRa q5 polymerase
    DiPaC of the sod BGC into pET28b-ptetO:: sod_gfp v2. a PCR amplification of the 4.6 kb sodA – D fragment with <t>Q5</t> polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO:: gfp v2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO:: sod_gfp v2 plasmid and e control restriction digest of two clones with Nde I or Eco RV in comparison to the predicted (Snapgene) pattern
    Q5 Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase/product/TaKaRa
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase - by Bioz Stars, 2020-07
    90/100 stars
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    92
    Thermo Fisher q5 polymerase
    DiPaC of the sod BGC into pET28b-ptetO:: sod_gfp v2. a PCR amplification of the 4.6 kb sodA – D fragment with <t>Q5</t> polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO:: gfp v2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO:: sod_gfp v2 plasmid and e control restriction digest of two clones with Nde I or Eco RV in comparison to the predicted (Snapgene) pattern
    Q5 Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase/product/Thermo Fisher
    Average 92 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Promega q5 polymerase
    DiPaC of the sod BGC into pET28b-ptetO:: sod_gfp v2. a PCR amplification of the 4.6 kb sodA – D fragment with <t>Q5</t> polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO:: gfp v2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO:: sod_gfp v2 plasmid and e control restriction digest of two clones with Nde I or Eco RV in comparison to the predicted (Snapgene) pattern
    Q5 Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase/product/Promega
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    99
    New England Biolabs q5 polymerase kit
    DiPaC of the sod BGC into pET28b-ptetO:: sod_gfp v2. a PCR amplification of the 4.6 kb sodA – D fragment with <t>Q5</t> polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO:: gfp v2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO:: sod_gfp v2 plasmid and e control restriction digest of two clones with Nde I or Eco RV in comparison to the predicted (Snapgene) pattern
    Q5 Polymerase Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase kit/product/New England Biolabs
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase kit - by Bioz Stars, 2020-07
    99/100 stars
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    90
    ATUM q5 polymerase
    DiPaC of the sod BGC into pET28b-ptetO:: sod_gfp v2. a PCR amplification of the 4.6 kb sodA – D fragment with <t>Q5</t> polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO:: gfp v2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO:: sod_gfp v2 plasmid and e control restriction digest of two clones with Nde I or Eco RV in comparison to the predicted (Snapgene) pattern
    Q5 Polymerase, supplied by ATUM, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase/product/ATUM
    Average 90 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase - by Bioz Stars, 2020-07
    90/100 stars
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    99
    New England Biolabs phusion q5 polymerase
    DiPaC of the sod BGC into pET28b-ptetO:: sod_gfp v2. a PCR amplification of the 4.6 kb sodA – D fragment with <t>Q5</t> polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO:: gfp v2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO:: sod_gfp v2 plasmid and e control restriction digest of two clones with Nde I or Eco RV in comparison to the predicted (Snapgene) pattern
    Phusion Q5 Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phusion q5 polymerase/product/New England Biolabs
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    phusion q5 polymerase - by Bioz Stars, 2020-07
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    92
    Stratagene q5 polymerase
    DiPaC of the sod BGC into pET28b-ptetO:: sod_gfp v2. a PCR amplification of the 4.6 kb sodA – D fragment with <t>Q5</t> polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO:: gfp v2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO:: sod_gfp v2 plasmid and e control restriction digest of two clones with Nde I or Eco RV in comparison to the predicted (Snapgene) pattern
    Q5 Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase/product/Stratagene
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase - by Bioz Stars, 2020-07
    92/100 stars
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    90
    New England Biolabs q5 polymerase mastermix
    DiPaC of the sod BGC into pET28b-ptetO:: sod_gfp v2. a PCR amplification of the 4.6 kb sodA – D fragment with <t>Q5</t> polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO:: gfp v2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO:: sod_gfp v2 plasmid and e control restriction digest of two clones with Nde I or Eco RV in comparison to the predicted (Snapgene) pattern
    Q5 Polymerase Mastermix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase mastermix/product/New England Biolabs
    Average 90 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase mastermix - by Bioz Stars, 2020-07
    90/100 stars
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    90
    New England Biolabs q5 polymerase buffer
    DiPaC of the sod BGC into pET28b-ptetO:: sod_gfp v2. a PCR amplification of the 4.6 kb sodA – D fragment with <t>Q5</t> polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO:: gfp v2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO:: sod_gfp v2 plasmid and e control restriction digest of two clones with Nde I or Eco RV in comparison to the predicted (Snapgene) pattern
    Q5 Polymerase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase buffer/product/New England Biolabs
    Average 90 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase buffer - by Bioz Stars, 2020-07
    90/100 stars
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    94
    New England Biolabs q5 polymerase reaction
    DiPaC of the sod BGC into pET28b-ptetO:: sod_gfp v2. a PCR amplification of the 4.6 kb sodA – D fragment with <t>Q5</t> polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO:: gfp v2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO:: sod_gfp v2 plasmid and e control restriction digest of two clones with Nde I or Eco RV in comparison to the predicted (Snapgene) pattern
    Q5 Polymerase Reaction, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 polymerase reaction/product/New England Biolabs
    Average 94 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    q5 polymerase reaction - by Bioz Stars, 2020-07
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    Image Search Results


    DiPaC of the sod BGC into pET28b-ptetO:: sod_gfp v2. a PCR amplification of the 4.6 kb sodA – D fragment with Q5 polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO:: gfp v2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO:: sod_gfp v2 plasmid and e control restriction digest of two clones with Nde I or Eco RV in comparison to the predicted (Snapgene) pattern

    Journal: Microbial Cell Factories

    Article Title: Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli

    doi: 10.1186/s12934-019-1080-6

    Figure Lengend Snippet: DiPaC of the sod BGC into pET28b-ptetO:: sod_gfp v2. a PCR amplification of the 4.6 kb sodA – D fragment with Q5 polymerase and primers containing homology sequences for SLIC into the backbone, b sodA–D fragment after gel purification, c PCR amplification to construct a linear 6.6 kb backbone of pET28b-ptetO:: gfp v2, d positive screening PCR of five clones after transformation of the SLIC-joined pET28b-ptetO:: sod_gfp v2 plasmid and e control restriction digest of two clones with Nde I or Eco RV in comparison to the predicted (Snapgene) pattern

    Article Snippet: Linearized PCR amplicons of both the vector backbone and the sodABCD fragment were generated using PCR with Q5 polymerase (NEB) under standard conditions with 50 ng gDNA per 25 µL reaction setup (see Additional file : Table S3 for primer sequences and chapter 2.1 for PCR setup).

    Techniques: Polymerase Chain Reaction, Amplification, Gel Purification, Construct, Clone Assay, Transformation Assay, Plasmid Preparation