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    New England Biolabs q5 high fidelity dna polymerase new england biolabs
    Assay specificity for PIK 3 CA mutations. Comparison of mutant PIK 3 CA detection in human control genomic <t>DNA</t> ( gDNA ) and cell‐free plasma DNA following pre‐amplification or no pre‐amplification using <t>Q5</t> High‐Fidelity DNA Polymerase (New England BioLabs) or TaqMan ® PreAmp Master Mix (Life Technologies). The level of blank (LoB) was 0.04% for the mutation E545K and for H1047R, 0.02% for E542K, and less than 0.01% for H1047L as shown by the graphs.
    Q5 High Fidelity Dna Polymerase New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2269 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs q5 hot start high fidelty dna polymerase new england biolabs cat
    Distribution of polymorphic sites in the SSU rDNA of Halteria grandinella , Blepharisma americanum and Strombidium stylifer amplified using <t>Q5</t> Hot Start High-Fidelity <t>DNA</t> polymerase. Polymorphic sites are indicated by short vertical lines. The squares, circles and triangles represent the three individuals of each species. The two-way arrows indicate the hypervariable regions of SSU rDNA and the lengths of sequences are to scale. Hal, Halteria grandinella ; Ble, Blepharisma americanum ; Str, Strombidium stylifer . (Online version in colour.)
    Q5 Hot Start High Fidelty Dna Polymerase New England Biolabs Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q5 hot start high fidelty dna polymerase new england biolabs cat/product/New England Biolabs
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    Distribution of polymorphic sites in the SSU rDNA of Halteria grandinella , Blepharisma americanum and Strombidium stylifer amplified using <t>Q5</t> Hot Start High-Fidelity <t>DNA</t> polymerase. Polymorphic sites are indicated by short vertical lines. The squares, circles and triangles represent the three individuals of each species. The two-way arrows indicate the hypervariable regions of SSU rDNA and the lengths of sequences are to scale. Hal, Halteria grandinella ; Ble, Blepharisma americanum ; Str, Strombidium stylifer . (Online version in colour.)
    B0202 Q5 High Fidelity Dna Polymerase New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Distribution of polymorphic sites in the SSU rDNA of Halteria grandinella , Blepharisma americanum and Strombidium stylifer amplified using <t>Q5</t> Hot Start High-Fidelity <t>DNA</t> polymerase. Polymorphic sites are indicated by short vertical lines. The squares, circles and triangles represent the three individuals of each species. The two-way arrows indicate the hypervariable regions of SSU rDNA and the lengths of sequences are to scale. Hal, Halteria grandinella ; Ble, Blepharisma americanum ; Str, Strombidium stylifer . (Online version in colour.)
    Protein Lobind Tubes Eppendorf 0030108116 Q5 High Fidelity Dna Polymerase New England Biolabs, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Assay specificity for PIK 3 CA mutations. Comparison of mutant PIK 3 CA detection in human control genomic DNA ( gDNA ) and cell‐free plasma DNA following pre‐amplification or no pre‐amplification using Q5 High‐Fidelity DNA Polymerase (New England BioLabs) or TaqMan ® PreAmp Master Mix (Life Technologies). The level of blank (LoB) was 0.04% for the mutation E545K and for H1047R, 0.02% for E542K, and less than 0.01% for H1047L as shown by the graphs.

    Journal: Molecular Oncology

    Article Title: Correlation between circulating cell‐free PIK3CA tumor DNA levels and treatment response in patients with PIK3CA‐mutated metastatic breast cancer

    doi: 10.1002/1878-0261.12305

    Figure Lengend Snippet: Assay specificity for PIK 3 CA mutations. Comparison of mutant PIK 3 CA detection in human control genomic DNA ( gDNA ) and cell‐free plasma DNA following pre‐amplification or no pre‐amplification using Q5 High‐Fidelity DNA Polymerase (New England BioLabs) or TaqMan ® PreAmp Master Mix (Life Technologies). The level of blank (LoB) was 0.04% for the mutation E545K and for H1047R, 0.02% for E542K, and less than 0.01% for H1047L as shown by the graphs.

    Article Snippet: To increase the sensitivity of the analysis, the remaining 75 μL serum DNA was subjected to 12 cycles of PCR pre‐amplification with Q5 High‐Fidelity DNA Polymerase (New England BioLabs, Ipswich, MA, USA) using a multiplex PIK3CA primer mix.

    Techniques: Mutagenesis, Amplification

    Optimization of EvaGreen- and Q5 DNA polymerase-based dNTP quantification. ( A-F ) Each critical reaction component and reaction temperature were varied and the outcome evaluated. ( G ) Assay conditions in graphs A to F . The standard curves were generated from end-point (1h) baseline-corrected fluorescence values.

    Journal: bioRxiv

    Article Title: A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye, and inhibitor-resistant high-fidelity DNA polymerase

    doi: 10.1101/2019.12.17.879122

    Figure Lengend Snippet: Optimization of EvaGreen- and Q5 DNA polymerase-based dNTP quantification. ( A-F ) Each critical reaction component and reaction temperature were varied and the outcome evaluated. ( G ) Assay conditions in graphs A to F . The standard curves were generated from end-point (1h) baseline-corrected fluorescence values.

    Article Snippet: Based on the lowest background signal, and reported ( ) inhibitor resistance in DNA extraction-free PCR, we chose to continue the optimization with Q5 DNA polymerase.

    Techniques: Generated, Fluorescence

    Long synthetic DNA oligonucleotides, an inhibitor-resistant high-fidelity DNA polymerase and EvaGreen detection chemistry allow quantification of dNTPs from mouse liver extracts. ( A ) Representative quantification of dTTP using the published fluorometric probe-hydrolysis-based assay. For clarity, curves from three lowest standard samples (0.31 to 0.08 pmol) are not shown. ( B ) Representative quantification of dTTP using Q5 DNA polymerase, long oligonucleotide template and EvaGreen detection chemistry. ( C - D ) dTTP signal generated by the fluorometric methods from mouse liver extracts with and without 0.5 pmol dTTP spike-in calibrant. The final extract volume was diluted to 80 µl per 40 mg of initial tissue weight. ( E - F ) Standard curves generated from the end-point baseline-corrected fluorescence values. The lowest y-axis value shows the background signal. Gray lines present the 95% confidence interval of the non-linear curve fit.

    Journal: bioRxiv

    Article Title: A sensitive assay for dNTPs based on long synthetic oligonucleotides, EvaGreen dye, and inhibitor-resistant high-fidelity DNA polymerase

    doi: 10.1101/2019.12.17.879122

    Figure Lengend Snippet: Long synthetic DNA oligonucleotides, an inhibitor-resistant high-fidelity DNA polymerase and EvaGreen detection chemistry allow quantification of dNTPs from mouse liver extracts. ( A ) Representative quantification of dTTP using the published fluorometric probe-hydrolysis-based assay. For clarity, curves from three lowest standard samples (0.31 to 0.08 pmol) are not shown. ( B ) Representative quantification of dTTP using Q5 DNA polymerase, long oligonucleotide template and EvaGreen detection chemistry. ( C - D ) dTTP signal generated by the fluorometric methods from mouse liver extracts with and without 0.5 pmol dTTP spike-in calibrant. The final extract volume was diluted to 80 µl per 40 mg of initial tissue weight. ( E - F ) Standard curves generated from the end-point baseline-corrected fluorescence values. The lowest y-axis value shows the background signal. Gray lines present the 95% confidence interval of the non-linear curve fit.

    Article Snippet: Based on the lowest background signal, and reported ( ) inhibitor resistance in DNA extraction-free PCR, we chose to continue the optimization with Q5 DNA polymerase.

    Techniques: Hydrolysis Assay, Generated, Fluorescence

    Olig2.5 inhibited archaeal family B DNA polymerases. An E. coli DH5α colony harbouring the 2.6 kb upstream sequence of Olig2 in pGL3-Basic vector was cultured and 1 μl of the culture was directly used as template for PCR. Olig2F and OligTSSR were used as primers to amplify the 2 kb fragment. Olig2.5 was added to see its inhibition to various DNAP. DNAP: DNA polymerase. LA: LA Taq DNAP. Q5: Q5 High-Fidelity DNAP. PS: PrimeSTAR HS DNAP. GXL: PrimeSTAR GXL DNAP. Phusion: Phusion High-Fidelity DNAP. Cobuddy: Cobuddy Super Fidelity DNAP. KOD: KOD DNAP.

    Journal: Scientific Reports

    Article Title: TT(N)mGCCTC inhibits archaeal family B DNA polymerases

    doi: 10.1038/s41598-018-20127-4

    Figure Lengend Snippet: Olig2.5 inhibited archaeal family B DNA polymerases. An E. coli DH5α colony harbouring the 2.6 kb upstream sequence of Olig2 in pGL3-Basic vector was cultured and 1 μl of the culture was directly used as template for PCR. Olig2F and OligTSSR were used as primers to amplify the 2 kb fragment. Olig2.5 was added to see its inhibition to various DNAP. DNAP: DNA polymerase. LA: LA Taq DNAP. Q5: Q5 High-Fidelity DNAP. PS: PrimeSTAR HS DNAP. GXL: PrimeSTAR GXL DNAP. Phusion: Phusion High-Fidelity DNAP. Cobuddy: Cobuddy Super Fidelity DNAP. KOD: KOD DNAP.

    Article Snippet: Phusion® High-Fidelity DNA Polymerase (Phusion) and Q5® High-Fidelity DNA Polymerase (Q5) were from New England Biolabs.

    Techniques: Sequencing, Plasmid Preparation, Cell Culture, Polymerase Chain Reaction, Inhibition

    Distribution of polymorphic sites in the SSU rDNA of Halteria grandinella , Blepharisma americanum and Strombidium stylifer amplified using Q5 Hot Start High-Fidelity DNA polymerase. Polymorphic sites are indicated by short vertical lines. The squares, circles and triangles represent the three individuals of each species. The two-way arrows indicate the hypervariable regions of SSU rDNA and the lengths of sequences are to scale. Hal, Halteria grandinella ; Ble, Blepharisma americanum ; Str, Strombidium stylifer . (Online version in colour.)

    Journal: Proceedings of the Royal Society B: Biological Sciences

    Article Title: Disentangling sources of variation in SSU rDNA sequences from single cell analyses of ciliates: impact of copy number variation and experimental error

    doi: 10.1098/rspb.2017.0425

    Figure Lengend Snippet: Distribution of polymorphic sites in the SSU rDNA of Halteria grandinella , Blepharisma americanum and Strombidium stylifer amplified using Q5 Hot Start High-Fidelity DNA polymerase. Polymorphic sites are indicated by short vertical lines. The squares, circles and triangles represent the three individuals of each species. The two-way arrows indicate the hypervariable regions of SSU rDNA and the lengths of sequences are to scale. Hal, Halteria grandinella ; Ble, Blepharisma americanum ; Str, Strombidium stylifer . (Online version in colour.)

    Article Snippet: Afterwards, PfuTurbo DNA polymerase (Cat. #600250, Agilent Technologies, USA), Q5 Hot Start High-Fidelity DNA Polymerase (Cat. #M0493 L, New England Biolabs, USA), ExTaq DNA polymerase (Cat. #RR001A & #RR003A, TaKaRa, Japan) and Taq DNA Polymerase (Cat. #EP0402, Thermo Fisher Scientific, USA) were used to amplify the SSU rDNA in the plasmid with universal primers [ ].

    Techniques: Amplification