q-exactive orbitrap mass spectrometer Search Results


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  • 99
    Thermo Fisher qexactive hybrid quadrupole orbitrap mass spectrometer
    Mass spectrum of ZMPSTE24 reveals zinc binding. (A) High-resolution <t>Orbitrap</t> <t>QExactive</t> mass spectrum of ZMPSTE24 released from a detergent (octyl glucose neopentyl glycol, OGNG) micelle. Adduct peaks at higher m/z are due to binding of lipids (POPG and cholesterol hemisuccinate) that co-purify with the protein. Inset: holo and apo -metallo ZMPSTE24 can be resolved (masses of 55579 Da and 55517 Da, respectively), confirming that Zn 2+ is bound to the majority of the protein population.
    Qexactive Hybrid Quadrupole Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qexactive hybrid quadrupole orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 109 article reviews
    Price from $9.99 to $1999.99
    qexactive hybrid quadrupole orbitrap mass spectrometer - by Bioz Stars, 2020-08
    99/100 stars
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    99
    Thermo Fisher qexactive plus quadrupole orbitrap mass spectrometer
    Mass spectrum of ZMPSTE24 reveals zinc binding. (A) High-resolution <t>Orbitrap</t> <t>QExactive</t> mass spectrum of ZMPSTE24 released from a detergent (octyl glucose neopentyl glycol, OGNG) micelle. Adduct peaks at higher m/z are due to binding of lipids (POPG and cholesterol hemisuccinate) that co-purify with the protein. Inset: holo and apo -metallo ZMPSTE24 can be resolved (masses of 55579 Da and 55517 Da, respectively), confirming that Zn 2+ is bound to the majority of the protein population.
    Qexactive Plus Quadrupole Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qexactive plus quadrupole orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    qexactive plus quadrupole orbitrap mass spectrometer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher qexactive hf quadrupole orbitrap mass spectrometer
    Workflow of the Serial-omics experiment. 10 mg of breast tumor tissue and 10 mg of normal mammary gland were harvested from the mouse and snap frozen separately in liquid nitrogen. The frozen tissue was ground to a powder over dry ice. The powder was solubilized in PBS and extracted with methyltert-butyl ether (MTBE), methanol and water. The upper non-polar liquid phase was collected, dried out and analyzed for untargeted lipidomics via DDA with a Thermo <t>QExactive</t> Plus high resolution <t>Orbitrap</t> mass spectrometer. The lower liquid phase was collected, dried out and analyzed with polarity switching for polar metabolomics via both targeted metabolomics (AB/SCIEX 5500 QTRAP hybrid triple quadrupole mass spectrometer) and untargeted metabolomics (high resolution Thermo QExactive HF Orbitrap). The precipitated protein pellet was re-suspended in sample buffer and separated via a SDS-PAGE gel, fractionated, digested with trypsin and enriched for phosphopeptides via TiO 2 . The digestion mixture and TiO 2 enriched phosphopeptides were analyzed by DDA in pos mode with a Thermo QExactive HF.
    Qexactive Hf Quadrupole Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qexactive hf quadrupole orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    qexactive hf quadrupole orbitrap mass spectrometer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    91
    Thermo Fisher qexactive hf hybrid quadrupole orbitrap mass spectrometer
    Workflow of the Serial-omics experiment. 10 mg of breast tumor tissue and 10 mg of normal mammary gland were harvested from the mouse and snap frozen separately in liquid nitrogen. The frozen tissue was ground to a powder over dry ice. The powder was solubilized in PBS and extracted with methyltert-butyl ether (MTBE), methanol and water. The upper non-polar liquid phase was collected, dried out and analyzed for untargeted lipidomics via DDA with a Thermo <t>QExactive</t> Plus high resolution <t>Orbitrap</t> mass spectrometer. The lower liquid phase was collected, dried out and analyzed with polarity switching for polar metabolomics via both targeted metabolomics (AB/SCIEX 5500 QTRAP hybrid triple quadrupole mass spectrometer) and untargeted metabolomics (high resolution Thermo QExactive HF Orbitrap). The precipitated protein pellet was re-suspended in sample buffer and separated via a SDS-PAGE gel, fractionated, digested with trypsin and enriched for phosphopeptides via TiO 2 . The digestion mixture and TiO 2 enriched phosphopeptides were analyzed by DDA in pos mode with a Thermo QExactive HF.
    Qexactive Hf Hybrid Quadrupole Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qexactive hf hybrid quadrupole orbitrap mass spectrometer/product/Thermo Fisher
    Average 91 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    qexactive hf hybrid quadrupole orbitrap mass spectrometer - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    99
    Thermo Fisher qexactive focus hybrid quadrupole orbitrap mass spectrometer
    Workflow of the Serial-omics experiment. 10 mg of breast tumor tissue and 10 mg of normal mammary gland were harvested from the mouse and snap frozen separately in liquid nitrogen. The frozen tissue was ground to a powder over dry ice. The powder was solubilized in PBS and extracted with methyltert-butyl ether (MTBE), methanol and water. The upper non-polar liquid phase was collected, dried out and analyzed for untargeted lipidomics via DDA with a Thermo <t>QExactive</t> Plus high resolution <t>Orbitrap</t> mass spectrometer. The lower liquid phase was collected, dried out and analyzed with polarity switching for polar metabolomics via both targeted metabolomics (AB/SCIEX 5500 QTRAP hybrid triple quadrupole mass spectrometer) and untargeted metabolomics (high resolution Thermo QExactive HF Orbitrap). The precipitated protein pellet was re-suspended in sample buffer and separated via a SDS-PAGE gel, fractionated, digested with trypsin and enriched for phosphopeptides via TiO 2 . The digestion mixture and TiO 2 enriched phosphopeptides were analyzed by DDA in pos mode with a Thermo QExactive HF.
    Qexactive Focus Hybrid Quadrupole Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qexactive focus hybrid quadrupole orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    qexactive focus hybrid quadrupole orbitrap mass spectrometer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher q exactive orbitrap mass spectrometer
    Workflow of TMT quantitative shotgun proteomic analysis. Proteins extracted from rice leaves were reduced, alkylased, precipitated and digested before being labelled with Tandem Mass Tags. Labelled samples were pooled, fractionated by SCX and then analysed on a Q Exactive <t>Orbitrap</t> mass spectrometer. Raw data generated from the mass spectrometer were processed using Proteome Discover v1.4 and Mascot. The search results were further analysed by the TMTPrePro software package.
    Q Exactive Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5723 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q exactive orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 5723 article reviews
    Price from $9.99 to $1999.99
    q exactive orbitrap mass spectrometer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher qexactive hf x hybrid quadrupole orbitrap mass spectrometer
    Workflow of TMT quantitative shotgun proteomic analysis. Proteins extracted from rice leaves were reduced, alkylased, precipitated and digested before being labelled with Tandem Mass Tags. Labelled samples were pooled, fractionated by SCX and then analysed on a Q Exactive <t>Orbitrap</t> mass spectrometer. Raw data generated from the mass spectrometer were processed using Proteome Discover v1.4 and Mascot. The search results were further analysed by the TMTPrePro software package.
    Qexactive Hf X Hybrid Quadrupole Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qexactive hf x hybrid quadrupole orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    qexactive hf x hybrid quadrupole orbitrap mass spectrometer - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher q exactive plus orbitrap mass spectrometer
    Representative MS-MS spectra of significantly altered proteins obtained from Q-ExactivePlus <t>Orbitrap</t> mass spectrometer study. (A) MS-MS spectra of Plasminogen (PLG) protein. (B) MS-MS spectra of Vitronectin (VTN) protein. (C) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to HI. (D) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to POLY lesions. (E) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to CR individuals. (F) Validation of Plasminogen and Vitronectin using western blotting image Ponceau stained image of the blot after transfer.
    Q Exactive Plus Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q exactive plus orbitrap mass spectrometer/product/Thermo Fisher
    Average 90 stars, based on 545 article reviews
    Price from $9.99 to $1999.99
    q exactive plus orbitrap mass spectrometer - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    Mass spectrum of ZMPSTE24 reveals zinc binding. (A) High-resolution Orbitrap QExactive mass spectrum of ZMPSTE24 released from a detergent (octyl glucose neopentyl glycol, OGNG) micelle. Adduct peaks at higher m/z are due to binding of lipids (POPG and cholesterol hemisuccinate) that co-purify with the protein. Inset: holo and apo -metallo ZMPSTE24 can be resolved (masses of 55579 Da and 55517 Da, respectively), confirming that Zn 2+ is bound to the majority of the protein population.

    Journal: Nature chemistry

    Article Title: Mass spectrometry captures off-target drug binding and provides mechanistic insights into the human metalloprotease ZMPSTE24

    doi: 10.1038/nchem.2591

    Figure Lengend Snippet: Mass spectrum of ZMPSTE24 reveals zinc binding. (A) High-resolution Orbitrap QExactive mass spectrum of ZMPSTE24 released from a detergent (octyl glucose neopentyl glycol, OGNG) micelle. Adduct peaks at higher m/z are due to binding of lipids (POPG and cholesterol hemisuccinate) that co-purify with the protein. Inset: holo and apo -metallo ZMPSTE24 can be resolved (masses of 55579 Da and 55517 Da, respectively), confirming that Zn 2+ is bound to the majority of the protein population.

    Article Snippet: QExactive High-resolution mass spectrometry was performed using a modified QExactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) optimised for transmission of high m/z ions (Heck Nat Methods) and analysis of membrane proteins .

    Techniques: Binding Assay

    Workflow of the Serial-omics experiment. 10 mg of breast tumor tissue and 10 mg of normal mammary gland were harvested from the mouse and snap frozen separately in liquid nitrogen. The frozen tissue was ground to a powder over dry ice. The powder was solubilized in PBS and extracted with methyltert-butyl ether (MTBE), methanol and water. The upper non-polar liquid phase was collected, dried out and analyzed for untargeted lipidomics via DDA with a Thermo QExactive Plus high resolution Orbitrap mass spectrometer. The lower liquid phase was collected, dried out and analyzed with polarity switching for polar metabolomics via both targeted metabolomics (AB/SCIEX 5500 QTRAP hybrid triple quadrupole mass spectrometer) and untargeted metabolomics (high resolution Thermo QExactive HF Orbitrap). The precipitated protein pellet was re-suspended in sample buffer and separated via a SDS-PAGE gel, fractionated, digested with trypsin and enriched for phosphopeptides via TiO 2 . The digestion mixture and TiO 2 enriched phosphopeptides were analyzed by DDA in pos mode with a Thermo QExactive HF.

    Journal: Scientific Reports

    Article Title: Serial-omics of P53−/−, Brca1−/− Mouse Breast Tumor and Normal Mammary Gland

    doi: 10.1038/s41598-017-15132-y

    Figure Lengend Snippet: Workflow of the Serial-omics experiment. 10 mg of breast tumor tissue and 10 mg of normal mammary gland were harvested from the mouse and snap frozen separately in liquid nitrogen. The frozen tissue was ground to a powder over dry ice. The powder was solubilized in PBS and extracted with methyltert-butyl ether (MTBE), methanol and water. The upper non-polar liquid phase was collected, dried out and analyzed for untargeted lipidomics via DDA with a Thermo QExactive Plus high resolution Orbitrap mass spectrometer. The lower liquid phase was collected, dried out and analyzed with polarity switching for polar metabolomics via both targeted metabolomics (AB/SCIEX 5500 QTRAP hybrid triple quadrupole mass spectrometer) and untargeted metabolomics (high resolution Thermo QExactive HF Orbitrap). The precipitated protein pellet was re-suspended in sample buffer and separated via a SDS-PAGE gel, fractionated, digested with trypsin and enriched for phosphopeptides via TiO 2 . The digestion mixture and TiO 2 enriched phosphopeptides were analyzed by DDA in pos mode with a Thermo QExactive HF.

    Article Snippet: The other half of the metabolite samples were re-suspended in 30 μL LC/MS grade water, 5 μL were analyzed by positive negative switching mode using a high resolution QExactive HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) via a Top 8 DDA.

    Techniques: Mass Spectrometry, SDS Page

    Workflow of TMT quantitative shotgun proteomic analysis. Proteins extracted from rice leaves were reduced, alkylased, precipitated and digested before being labelled with Tandem Mass Tags. Labelled samples were pooled, fractionated by SCX and then analysed on a Q Exactive Orbitrap mass spectrometer. Raw data generated from the mass spectrometer were processed using Proteome Discover v1.4 and Mascot. The search results were further analysed by the TMTPrePro software package.

    Journal: Data in Brief

    Article Title: Label-free and isobaric tandem mass tag (TMT) multiplexed quantitative proteomic data of two contrasting rice cultivars exposed to drought stress and recovery

    doi: 10.1016/j.dib.2018.12.041

    Figure Lengend Snippet: Workflow of TMT quantitative shotgun proteomic analysis. Proteins extracted from rice leaves were reduced, alkylased, precipitated and digested before being labelled with Tandem Mass Tags. Labelled samples were pooled, fractionated by SCX and then analysed on a Q Exactive Orbitrap mass spectrometer. Raw data generated from the mass spectrometer were processed using Proteome Discover v1.4 and Mascot. The search results were further analysed by the TMTPrePro software package.

    Article Snippet: These fractions were desalted using C18 OMIX® tips (Agilent) and analysed on a Q Exactive Orbitrap mass spectrometer (Thermo Scientific) coupled to an EASY-nLC1000 (Thermo Scientific).

    Techniques: Mass Spectrometry, Generated, Software

    Representative MS-MS spectra of significantly altered proteins obtained from Q-ExactivePlus Orbitrap mass spectrometer study. (A) MS-MS spectra of Plasminogen (PLG) protein. (B) MS-MS spectra of Vitronectin (VTN) protein. (C) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to HI. (D) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to POLY lesions. (E) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to CR individuals. (F) Validation of Plasminogen and Vitronectin using western blotting image Ponceau stained image of the blot after transfer.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Clinical Proteomics Profiling for Biomarker Identification Among Patients Suffering With Indian Post Kala Azar Dermal Leishmaniasis

    doi: 10.3389/fcimb.2020.00251

    Figure Lengend Snippet: Representative MS-MS spectra of significantly altered proteins obtained from Q-ExactivePlus Orbitrap mass spectrometer study. (A) MS-MS spectra of Plasminogen (PLG) protein. (B) MS-MS spectra of Vitronectin (VTN) protein. (C) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to HI. (D) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to POLY lesions. (E) Venn diagram showing number of common proteins in all the patients with MAC lesions compared to CR individuals. (F) Validation of Plasminogen and Vitronectin using western blotting image Ponceau stained image of the blot after transfer.

    Article Snippet: Peptides were analyzed by electrospray ionization mass spectrometry using the Easy nano LC 1200 system [Thermo Scientific] coupled to a Q-Exactive Plus Orbitrap mass spectrometer [Thermo Scientific].

    Techniques: Tandem Mass Spectroscopy, Mass Spectrometry, Western Blot, Staining