q-exactive mass spectrometer Search Results


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  • 99
    Thermo Fisher q exactive mass spectrometer
    Q Exactive Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11450 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q exactive mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 11450 article reviews
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    Thermo Fisher q exactive plus mass spectrometer
    Mass spectrometry analysis of the exosomal proteins. To evaluate the compatibility of exosomes preps for proteomics study, the isolated exosome samples were dried using speed-vac and suspended into 20 µl of 8 M urea to solubilize membrane proteins followed by sonication. Subsequently, samples were incubated at −80 °C for 30 min and diluted four times by adding 60 µl of 50 mM Ammonium biocarbonate (ABC) and 10 mM Tris(2-carboxyethyl)phosphine (TCEP). Overnight digestion was done at 37 °C with sequencing grade trypsin. Samples were subjected to 1 h MS analysis on Thermo <t>QExactive</t> Plus mass spectrometer.
    Q Exactive Plus Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q exactive plus mass spectrometer/product/Thermo Fisher
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    Thermo Fisher q exactive hf mass spectrometer
    Mass spectrometry analysis of the exosomal proteins. To evaluate the compatibility of exosomes preps for proteomics study, the isolated exosome samples were dried using speed-vac and suspended into 20 µl of 8 M urea to solubilize membrane proteins followed by sonication. Subsequently, samples were incubated at −80 °C for 30 min and diluted four times by adding 60 µl of 50 mM Ammonium biocarbonate (ABC) and 10 mM Tris(2-carboxyethyl)phosphine (TCEP). Overnight digestion was done at 37 °C with sequencing grade trypsin. Samples were subjected to 1 h MS analysis on Thermo <t>QExactive</t> Plus mass spectrometer.
    Q Exactive Hf Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1981 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher q exactive orbitrap mass spectrometer
    Mass spectrometry analysis of the exosomal proteins. To evaluate the compatibility of exosomes preps for proteomics study, the isolated exosome samples were dried using speed-vac and suspended into 20 µl of 8 M urea to solubilize membrane proteins followed by sonication. Subsequently, samples were incubated at −80 °C for 30 min and diluted four times by adding 60 µl of 50 mM Ammonium biocarbonate (ABC) and 10 mM Tris(2-carboxyethyl)phosphine (TCEP). Overnight digestion was done at 37 °C with sequencing grade trypsin. Samples were subjected to 1 h MS analysis on Thermo <t>QExactive</t> Plus mass spectrometer.
    Q Exactive Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher q exactive hybrid quadrupole orbitrap mass spectrometer
    Mass spectrometry analysis of the exosomal proteins. To evaluate the compatibility of exosomes preps for proteomics study, the isolated exosome samples were dried using speed-vac and suspended into 20 µl of 8 M urea to solubilize membrane proteins followed by sonication. Subsequently, samples were incubated at −80 °C for 30 min and diluted four times by adding 60 µl of 50 mM Ammonium biocarbonate (ABC) and 10 mM Tris(2-carboxyethyl)phosphine (TCEP). Overnight digestion was done at 37 °C with sequencing grade trypsin. Samples were subjected to 1 h MS analysis on Thermo <t>QExactive</t> Plus mass spectrometer.
    Q Exactive Hybrid Quadrupole Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2763 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    q exactive hybrid quadrupole orbitrap mass spectrometer - by Bioz Stars, 2020-09
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    Thermo Fisher qexactive mass spectrometer
    Mass spectrometry analysis of the exosomal proteins. To evaluate the compatibility of exosomes preps for proteomics study, the isolated exosome samples were dried using speed-vac and suspended into 20 µl of 8 M urea to solubilize membrane proteins followed by sonication. Subsequently, samples were incubated at −80 °C for 30 min and diluted four times by adding 60 µl of 50 mM Ammonium biocarbonate (ABC) and 10 mM Tris(2-carboxyethyl)phosphine (TCEP). Overnight digestion was done at 37 °C with sequencing grade trypsin. Samples were subjected to 1 h MS analysis on Thermo <t>QExactive</t> Plus mass spectrometer.
    Qexactive Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qexactive mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 1149 article reviews
    Price from $9.99 to $1999.99
    qexactive mass spectrometer - by Bioz Stars, 2020-09
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    99
    Thermo Fisher q exactive plus hybrid quadrupole orbitrap mass spectrometer
    Mass spectrometry analysis of the exosomal proteins. To evaluate the compatibility of exosomes preps for proteomics study, the isolated exosome samples were dried using speed-vac and suspended into 20 µl of 8 M urea to solubilize membrane proteins followed by sonication. Subsequently, samples were incubated at −80 °C for 30 min and diluted four times by adding 60 µl of 50 mM Ammonium biocarbonate (ABC) and 10 mM Tris(2-carboxyethyl)phosphine (TCEP). Overnight digestion was done at 37 °C with sequencing grade trypsin. Samples were subjected to 1 h MS analysis on Thermo <t>QExactive</t> Plus mass spectrometer.
    Q Exactive Plus Hybrid Quadrupole Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 749 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q exactive plus hybrid quadrupole orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 749 article reviews
    Price from $9.99 to $1999.99
    q exactive plus hybrid quadrupole orbitrap mass spectrometer - by Bioz Stars, 2020-09
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    Thermo Fisher q exactive plus orbitrap mass spectrometer
    Mass spectrometry analysis of the exosomal proteins. To evaluate the compatibility of exosomes preps for proteomics study, the isolated exosome samples were dried using speed-vac and suspended into 20 µl of 8 M urea to solubilize membrane proteins followed by sonication. Subsequently, samples were incubated at −80 °C for 30 min and diluted four times by adding 60 µl of 50 mM Ammonium biocarbonate (ABC) and 10 mM Tris(2-carboxyethyl)phosphine (TCEP). Overnight digestion was done at 37 °C with sequencing grade trypsin. Samples were subjected to 1 h MS analysis on Thermo <t>QExactive</t> Plus mass spectrometer.
    Q Exactive Plus Orbitrap Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 638 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q exactive plus orbitrap mass spectrometer/product/Thermo Fisher
    Average 99 stars, based on 638 article reviews
    Price from $9.99 to $1999.99
    q exactive plus orbitrap mass spectrometer - by Bioz Stars, 2020-09
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    Thermo Fisher q exactive high resolution accurate mass spectrometer
    Mass spectrometry analysis of the exosomal proteins. To evaluate the compatibility of exosomes preps for proteomics study, the isolated exosome samples were dried using speed-vac and suspended into 20 µl of 8 M urea to solubilize membrane proteins followed by sonication. Subsequently, samples were incubated at −80 °C for 30 min and diluted four times by adding 60 µl of 50 mM Ammonium biocarbonate (ABC) and 10 mM Tris(2-carboxyethyl)phosphine (TCEP). Overnight digestion was done at 37 °C with sequencing grade trypsin. Samples were subjected to 1 h MS analysis on Thermo <t>QExactive</t> Plus mass spectrometer.
    Q Exactive High Resolution Accurate Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 494 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/q exactive high resolution accurate mass spectrometer/product/Thermo Fisher
    Average 94 stars, based on 494 article reviews
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    q exactive high resolution accurate mass spectrometer - by Bioz Stars, 2020-09
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    Thermo Fisher orbitrap q exactive mass spectrometer
    Mass spectrometry analysis of the exosomal proteins. To evaluate the compatibility of exosomes preps for proteomics study, the isolated exosome samples were dried using speed-vac and suspended into 20 µl of 8 M urea to solubilize membrane proteins followed by sonication. Subsequently, samples were incubated at −80 °C for 30 min and diluted four times by adding 60 µl of 50 mM Ammonium biocarbonate (ABC) and 10 mM Tris(2-carboxyethyl)phosphine (TCEP). Overnight digestion was done at 37 °C with sequencing grade trypsin. Samples were subjected to 1 h MS analysis on Thermo <t>QExactive</t> Plus mass spectrometer.
    Orbitrap Q Exactive Mass Spectrometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 482 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Mass spectrometry analysis of the exosomal proteins. To evaluate the compatibility of exosomes preps for proteomics study, the isolated exosome samples were dried using speed-vac and suspended into 20 µl of 8 M urea to solubilize membrane proteins followed by sonication. Subsequently, samples were incubated at −80 °C for 30 min and diluted four times by adding 60 µl of 50 mM Ammonium biocarbonate (ABC) and 10 mM Tris(2-carboxyethyl)phosphine (TCEP). Overnight digestion was done at 37 °C with sequencing grade trypsin. Samples were subjected to 1 h MS analysis on Thermo QExactive Plus mass spectrometer.

    Journal: Scientific Reports

    Article Title: Comparative analysis of exosome isolation methods using culture supernatant for optimum yield, purity and downstream applications

    doi: 10.1038/s41598-019-41800-2

    Figure Lengend Snippet: Mass spectrometry analysis of the exosomal proteins. To evaluate the compatibility of exosomes preps for proteomics study, the isolated exosome samples were dried using speed-vac and suspended into 20 µl of 8 M urea to solubilize membrane proteins followed by sonication. Subsequently, samples were incubated at −80 °C for 30 min and diluted four times by adding 60 µl of 50 mM Ammonium biocarbonate (ABC) and 10 mM Tris(2-carboxyethyl)phosphine (TCEP). Overnight digestion was done at 37 °C with sequencing grade trypsin. Samples were subjected to 1 h MS analysis on Thermo QExactive Plus mass spectrometer.

    Article Snippet: Each sample subjected to a 1 h mass spectrometry analysis on Thermo QExactive Plus mass spectrometer for qualitative analysis to check the downstream application of exosomes for mass spectroscopy.

    Techniques: Mass Spectrometry, Isolation, Sonication, Incubation, Sequencing

    Workflow for evaluation of DNA damage response of HA-RelA U2OS cells with either Etoposide (ETO) or Hydroxyurea (HU). ( A ) HA-RelA U2OS cells were treated with either 0.1% DMSO (control), ETO (50 μM) or HU (2 mM) for the times indicated to induce DNA damage. ( B ) Following treatment with either ETO or HU, HA-RelA U2OS cell lysates were immunoblotted with antibodies against either γH2AX as a marker of DNA damage, or RelA/p65. ( C ) Workflow for evaluation of the phosphorylated RelA interactome. Cells were treated as in ( A ), and RelA-bound proteins immunoprecipitated with an anti-HA antibody. Proteins were subjected to tryptic digestion and TiO 2 -based phosphopeptide enrichment. ( D ) Peptides were analysed by LC-MS/MS using a QExactive HF and subjected to label-free quantification following appropriate normalisation.

    Journal: bioRxiv

    Article Title: Temporal modulation of the NF-κB RelA network in response to different types of DNA damage

    doi: 10.1101/2020.08.11.246504

    Figure Lengend Snippet: Workflow for evaluation of DNA damage response of HA-RelA U2OS cells with either Etoposide (ETO) or Hydroxyurea (HU). ( A ) HA-RelA U2OS cells were treated with either 0.1% DMSO (control), ETO (50 μM) or HU (2 mM) for the times indicated to induce DNA damage. ( B ) Following treatment with either ETO or HU, HA-RelA U2OS cell lysates were immunoblotted with antibodies against either γH2AX as a marker of DNA damage, or RelA/p65. ( C ) Workflow for evaluation of the phosphorylated RelA interactome. Cells were treated as in ( A ), and RelA-bound proteins immunoprecipitated with an anti-HA antibody. Proteins were subjected to tryptic digestion and TiO 2 -based phosphopeptide enrichment. ( D ) Peptides were analysed by LC-MS/MS using a QExactive HF and subjected to label-free quantification following appropriate normalisation.

    Article Snippet: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysisPhosphopeptide enriched samples were analysed by LC-MS/MS using an Ultimate 3000 RSLC™ nano system (Thermo Scientific, Hemel Hempstead) coupled to a QExactive HF mass spectrometer (Thermo Scientific).

    Techniques: Marker, Immunoprecipitation, Liquid Chromatography with Mass Spectroscopy