pyrf Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Addgene inc us0hisb pyrf
    Us0hisb Pyrf, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/us0hisb pyrf/product/Addgene inc
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    us0hisb pyrf - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    93
    TaKaRa pmat1552 pyrf te 04
    ( A , B ) Expression of TE-01 , TE-02 , TE-03 , and <t>TE-04</t> genes. ( A ) Structure of plasmids <t>pMAT1552-pyrF-TE-01,</t> pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 for TE-01 , TE-02 , TE-03 , TE-04 genes over-expressing in M. circinelloides M65 are demonstrated. Green boxes indicate the coding region of thioesterase genes. ( B ) Polymerase chain reaction (PCR) amplification of genome of control strain (A) and thioesterase gene (TE) over-expressing strains i.e., M65-TE-01, M65-TE-02, M65-TE-03, and M65-TE-04 was demonstrated to as B–E, respectively with the primers ( Supplementary materials: Table S2 ). M, Gene Ruler DNA Ladder Mix. Sizes in kb of the relevant maker fragments are indicated.
    Pmat1552 Pyrf Te 04, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmat1552 pyrf te 04/product/TaKaRa
    Average 93 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    pmat1552 pyrf te 04 - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    91
    Marker Gene Technologies pyrf marker gene
    ( A , B ) Expression of TE-01 , TE-02 , TE-03 , and <t>TE-04</t> genes. ( A ) Structure of plasmids <t>pMAT1552-pyrF-TE-01,</t> pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 for TE-01 , TE-02 , TE-03 , TE-04 genes over-expressing in M. circinelloides M65 are demonstrated. Green boxes indicate the coding region of thioesterase genes. ( B ) Polymerase chain reaction (PCR) amplification of genome of control strain (A) and thioesterase gene (TE) over-expressing strains i.e., M65-TE-01, M65-TE-02, M65-TE-03, and M65-TE-04 was demonstrated to as B–E, respectively with the primers ( Supplementary materials: Table S2 ). M, Gene Ruler DNA Ladder Mix. Sizes in kb of the relevant maker fragments are indicated.
    Pyrf Marker Gene, supplied by Marker Gene Technologies, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pyrf marker gene/product/Marker Gene Technologies
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pyrf marker gene - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    92
    Marker Gene Technologies pyrf dpl200 marker gene
    Characterization of spontaneous <t>pyrF</t> - mutants of Rhizopus delemar FGSC-9543 and CDC-8219 strains. (A) Typical appearance of spontaneous 5-FOA resistant colonies of CDC-8219 after incubation for 5 days at 30 °C. The typical pyrF - mutant is pointed by a white arrow. The colony pointed by the black arrow typically failed to retain 5-FOA resistance in subsequent culturing. (B) DNA sequencing of the 3.0-kb fragment amplified by primers PW1819 and PW1827 that contains the pyrF 5′-UTR and ORF. (C) Growth of the R. delemar pyrF - f2 mutant in comparison to wild type CDC-8219 and complemented TF1 and TF3 strains on potato dextrose agar (PDA), MMC –uracil, and MMC + 5-FOA (1 g/L). Incubation was carried out at 30°C for 1–5 days as indicated. (D) DNA sequencing of the 217-bp PCR fragment amplified with primers PW1972 and PW1945 (top chromatograms) and cloned products (lower chromatograms). (E) Primers PW2015 and PW2050 were used for PCR (30 cycles for the <t>pyrF-dpl200</t> control and 35 cycles for the rest) to analyze complemented pyrF - mutant strains. A ~400-bp is expected for the pyrF - mutant and ~600-bp is expected for the integrated pyrF-dpl200 marker. Lane 1: pyrF-dpl200 , lane 2: pyrF (91–92ΔTC), lane 3 and 4: complemented TF #1 and TF #3, respectively.
    Pyrf Dpl200 Marker Gene, supplied by Marker Gene Technologies, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pyrf dpl200 marker gene/product/Marker Gene Technologies
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    pyrf dpl200 marker gene - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Addgene inc e coli strain us0 hisb pyrf rpoz
    Characterization of spontaneous <t>pyrF</t> - mutants of Rhizopus delemar FGSC-9543 and CDC-8219 strains. (A) Typical appearance of spontaneous 5-FOA resistant colonies of CDC-8219 after incubation for 5 days at 30 °C. The typical pyrF - mutant is pointed by a white arrow. The colony pointed by the black arrow typically failed to retain 5-FOA resistance in subsequent culturing. (B) DNA sequencing of the 3.0-kb fragment amplified by primers PW1819 and PW1827 that contains the pyrF 5′-UTR and ORF. (C) Growth of the R. delemar pyrF - f2 mutant in comparison to wild type CDC-8219 and complemented TF1 and TF3 strains on potato dextrose agar (PDA), MMC –uracil, and MMC + 5-FOA (1 g/L). Incubation was carried out at 30°C for 1–5 days as indicated. (D) DNA sequencing of the 217-bp PCR fragment amplified with primers PW1972 and PW1945 (top chromatograms) and cloned products (lower chromatograms). (E) Primers PW2015 and PW2050 were used for PCR (30 cycles for the <t>pyrF-dpl200</t> control and 35 cycles for the rest) to analyze complemented pyrF - mutant strains. A ~400-bp is expected for the pyrF - mutant and ~600-bp is expected for the integrated pyrF-dpl200 marker. Lane 1: pyrF-dpl200 , lane 2: pyrF (91–92ΔTC), lane 3 and 4: complemented TF #1 and TF #3, respectively.
    E Coli Strain Us0 Hisb Pyrf Rpoz, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli strain us0 hisb pyrf rpoz/product/Addgene inc
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    e coli strain us0 hisb pyrf rpoz - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    ( A , B ) Expression of TE-01 , TE-02 , TE-03 , and TE-04 genes. ( A ) Structure of plasmids pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 for TE-01 , TE-02 , TE-03 , TE-04 genes over-expressing in M. circinelloides M65 are demonstrated. Green boxes indicate the coding region of thioesterase genes. ( B ) Polymerase chain reaction (PCR) amplification of genome of control strain (A) and thioesterase gene (TE) over-expressing strains i.e., M65-TE-01, M65-TE-02, M65-TE-03, and M65-TE-04 was demonstrated to as B–E, respectively with the primers ( Supplementary materials: Table S2 ). M, Gene Ruler DNA Ladder Mix. Sizes in kb of the relevant maker fragments are indicated.

    Journal: International Journal of Molecular Sciences

    Article Title: Engineering of Fatty Acid Synthases (FASs) to Boost the Production of Medium-Chain Fatty Acids (MCFAs) in Mucor circinelloides

    doi: 10.3390/ijms20030786

    Figure Lengend Snippet: ( A , B ) Expression of TE-01 , TE-02 , TE-03 , and TE-04 genes. ( A ) Structure of plasmids pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 for TE-01 , TE-02 , TE-03 , TE-04 genes over-expressing in M. circinelloides M65 are demonstrated. Green boxes indicate the coding region of thioesterase genes. ( B ) Polymerase chain reaction (PCR) amplification of genome of control strain (A) and thioesterase gene (TE) over-expressing strains i.e., M65-TE-01, M65-TE-02, M65-TE-03, and M65-TE-04 was demonstrated to as B–E, respectively with the primers ( Supplementary materials: Table S2 ). M, Gene Ruler DNA Ladder Mix. Sizes in kb of the relevant maker fragments are indicated.

    Article Snippet: The aforesaid primers exhibited homologous sequences (i.e., 28 bp) to the Xho I restriction sites in plasmid pMAT1552-pyrF, and the PCR fragment was finally cloned into plasmid pMAT1552-pyrF using the restriction endonuclease Xho I to construct the recombinant plasmids i.e., pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 (One-Step Cloning Kit from Takara Bio USA, Inc., CA, USA).

    Techniques: Expressing, Polymerase Chain Reaction, Amplification

    Characterization of spontaneous pyrF - mutants of Rhizopus delemar FGSC-9543 and CDC-8219 strains. (A) Typical appearance of spontaneous 5-FOA resistant colonies of CDC-8219 after incubation for 5 days at 30 °C. The typical pyrF - mutant is pointed by a white arrow. The colony pointed by the black arrow typically failed to retain 5-FOA resistance in subsequent culturing. (B) DNA sequencing of the 3.0-kb fragment amplified by primers PW1819 and PW1827 that contains the pyrF 5′-UTR and ORF. (C) Growth of the R. delemar pyrF - f2 mutant in comparison to wild type CDC-8219 and complemented TF1 and TF3 strains on potato dextrose agar (PDA), MMC –uracil, and MMC + 5-FOA (1 g/L). Incubation was carried out at 30°C for 1–5 days as indicated. (D) DNA sequencing of the 217-bp PCR fragment amplified with primers PW1972 and PW1945 (top chromatograms) and cloned products (lower chromatograms). (E) Primers PW2015 and PW2050 were used for PCR (30 cycles for the pyrF-dpl200 control and 35 cycles for the rest) to analyze complemented pyrF - mutant strains. A ~400-bp is expected for the pyrF - mutant and ~600-bp is expected for the integrated pyrF-dpl200 marker. Lane 1: pyrF-dpl200 , lane 2: pyrF (91–92ΔTC), lane 3 and 4: complemented TF #1 and TF #3, respectively.

    Journal: Fungal genetics and biology : FG & B

    Article Title: CRISPR-Cas9 induces point mutation in the mucormycosis fungus Rhizopus delemar

    doi: 10.1016/j.fgb.2018.12.002

    Figure Lengend Snippet: Characterization of spontaneous pyrF - mutants of Rhizopus delemar FGSC-9543 and CDC-8219 strains. (A) Typical appearance of spontaneous 5-FOA resistant colonies of CDC-8219 after incubation for 5 days at 30 °C. The typical pyrF - mutant is pointed by a white arrow. The colony pointed by the black arrow typically failed to retain 5-FOA resistance in subsequent culturing. (B) DNA sequencing of the 3.0-kb fragment amplified by primers PW1819 and PW1827 that contains the pyrF 5′-UTR and ORF. (C) Growth of the R. delemar pyrF - f2 mutant in comparison to wild type CDC-8219 and complemented TF1 and TF3 strains on potato dextrose agar (PDA), MMC –uracil, and MMC + 5-FOA (1 g/L). Incubation was carried out at 30°C for 1–5 days as indicated. (D) DNA sequencing of the 217-bp PCR fragment amplified with primers PW1972 and PW1945 (top chromatograms) and cloned products (lower chromatograms). (E) Primers PW2015 and PW2050 were used for PCR (30 cycles for the pyrF-dpl200 control and 35 cycles for the rest) to analyze complemented pyrF - mutant strains. A ~400-bp is expected for the pyrF - mutant and ~600-bp is expected for the integrated pyrF-dpl200 marker. Lane 1: pyrF-dpl200 , lane 2: pyrF (91–92ΔTC), lane 3 and 4: complemented TF #1 and TF #3, respectively.

    Article Snippet: A 200-bp fragment from the 5′ UTR of the R. delemar pyrF gene was tandemly incorporated into the downstream, generating the pyrF-dpl200 marker gene ( ).

    Techniques: Incubation, Mutagenesis, DNA Sequencing, Amplification, Polymerase Chain Reaction, Clone Assay, Marker