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  • 86
    Bio-Synthesis Inc pyrf marker gene
    Pyrf Marker Gene, supplied by Bio-Synthesis Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pyrf marker gene/product/Bio-Synthesis Inc
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    92
    Addgene inc e coli strain us0 hisb pyrf rpoz
    E Coli Strain Us0 Hisb Pyrf Rpoz, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa pmat1552 pyrf te 04
    ( A , B ) Expression of TE-01 , TE-02 , TE-03 , and <t>TE-04</t> genes. ( A ) Structure of plasmids <t>pMAT1552-pyrF-TE-01,</t> pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 for TE-01 , TE-02 , TE-03 , TE-04 genes over-expressing in M. circinelloides M65 are demonstrated. Green boxes indicate the coding region of thioesterase genes. ( B ) Polymerase chain reaction (PCR) amplification of genome of control strain (A) and thioesterase gene (TE) over-expressing strains i.e., M65-TE-01, M65-TE-02, M65-TE-03, and M65-TE-04 was demonstrated to as B–E, respectively with the primers ( Supplementary materials: Table S2 ). M, Gene Ruler DNA Ladder Mix. Sizes in kb of the relevant maker fragments are indicated.
    Pmat1552 Pyrf Te 04, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A , B ) Expression of TE-01 , TE-02 , TE-03 , and TE-04 genes. ( A ) Structure of plasmids pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 for TE-01 , TE-02 , TE-03 , TE-04 genes over-expressing in M. circinelloides M65 are demonstrated. Green boxes indicate the coding region of thioesterase genes. ( B ) Polymerase chain reaction (PCR) amplification of genome of control strain (A) and thioesterase gene (TE) over-expressing strains i.e., M65-TE-01, M65-TE-02, M65-TE-03, and M65-TE-04 was demonstrated to as B–E, respectively with the primers ( Supplementary materials: Table S2 ). M, Gene Ruler DNA Ladder Mix. Sizes in kb of the relevant maker fragments are indicated.

    Journal: International Journal of Molecular Sciences

    Article Title: Engineering of Fatty Acid Synthases (FASs) to Boost the Production of Medium-Chain Fatty Acids (MCFAs) in Mucor circinelloides

    doi: 10.3390/ijms20030786

    Figure Lengend Snippet: ( A , B ) Expression of TE-01 , TE-02 , TE-03 , and TE-04 genes. ( A ) Structure of plasmids pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 for TE-01 , TE-02 , TE-03 , TE-04 genes over-expressing in M. circinelloides M65 are demonstrated. Green boxes indicate the coding region of thioesterase genes. ( B ) Polymerase chain reaction (PCR) amplification of genome of control strain (A) and thioesterase gene (TE) over-expressing strains i.e., M65-TE-01, M65-TE-02, M65-TE-03, and M65-TE-04 was demonstrated to as B–E, respectively with the primers ( Supplementary materials: Table S2 ). M, Gene Ruler DNA Ladder Mix. Sizes in kb of the relevant maker fragments are indicated.

    Article Snippet: The aforesaid primers exhibited homologous sequences (i.e., 28 bp) to the Xho I restriction sites in plasmid pMAT1552-pyrF, and the PCR fragment was finally cloned into plasmid pMAT1552-pyrF using the restriction endonuclease Xho I to construct the recombinant plasmids i.e., pMAT1552-pyrF-TE-01, pMAT1552-pyrF-TE-02, pMAT1552-pyrF-TE-03, pMAT1552-pyrF-TE-04 (One-Step Cloning Kit from Takara Bio USA, Inc., CA, USA).

    Techniques: Expressing, Polymerase Chain Reaction, Amplification