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  • 96
    Millipore polyvinylidene difluoride membranes
    Polyvinylidene Difluoride Membranes, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyvinylidene difluoride membranes/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyvinylidene difluoride membranes - by Bioz Stars, 2021-07
    96/100 stars
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    99
    Bio-Rad pvdf membrane
    Pvdf Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pvdf membrane/product/Bio-Rad
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pvdf membrane - by Bioz Stars, 2021-07
    99/100 stars
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    99
    Millipore polyvinylidene difluoride pvdf membranes
    Polyvinylidene Difluoride Pvdf Membranes, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyvinylidene difluoride pvdf membranes/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyvinylidene difluoride pvdf membranes - by Bioz Stars, 2021-07
    99/100 stars
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    86
    GE Healthcare immobilon p membranes
    METTL1 is phosphorylated in mouse ES cells expressing wild-type PDK1 or PDK1[L155E], but not in PDK1 null cells. Mouse ES cells expressing PDK1[+/+], PDK1[L155E/L155E] or PDK1[−/−] were serum starved for 4 h, incubated for 20 min without (−) or with (+) 100 nM wortmannin, and then stimulated for a further 15 min with 20 ng/ml IGF-1. METTL1 was immunoprecipitated from 2.5 mg cell lysate protein using the antibody raised against mouse METTL1 residues 258–268, denatured in SDS, subjected to SDS–PAGE and, after transfer to <t>Immobilon-P</t> membranes, immunoblotted as in panel A.
    Immobilon P Membranes, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immobilon p membranes/product/GE Healthcare
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immobilon p membranes - by Bioz Stars, 2021-07
    86/100 stars
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    N/A
    Usable from 32 to 212ºF 0 to 100ºC
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    N/A
    Category Antibodies Other Reagents PVDF Membrane 0 45 μm Size 8 5cm×6cm×10pieces Price 50
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    N/A
    PVDF Sanitary Barbed Adapter 3 4 CLAMP X 5 8 Tubing ID
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    Image Search Results


    METTL1 is phosphorylated in mouse ES cells expressing wild-type PDK1 or PDK1[L155E], but not in PDK1 null cells. Mouse ES cells expressing PDK1[+/+], PDK1[L155E/L155E] or PDK1[−/−] were serum starved for 4 h, incubated for 20 min without (−) or with (+) 100 nM wortmannin, and then stimulated for a further 15 min with 20 ng/ml IGF-1. METTL1 was immunoprecipitated from 2.5 mg cell lysate protein using the antibody raised against mouse METTL1 residues 258–268, denatured in SDS, subjected to SDS–PAGE and, after transfer to Immobilon-P membranes, immunoblotted as in panel A.

    Journal: The EMBO Journal

    Article Title: The tRNA methylase METTL1 is phosphorylated and inactivated by PKB and RSK in vitro and in cells

    doi: 10.1038/sj.emboj.7600648

    Figure Lengend Snippet: METTL1 is phosphorylated in mouse ES cells expressing wild-type PDK1 or PDK1[L155E], but not in PDK1 null cells. Mouse ES cells expressing PDK1[+/+], PDK1[L155E/L155E] or PDK1[−/−] were serum starved for 4 h, incubated for 20 min without (−) or with (+) 100 nM wortmannin, and then stimulated for a further 15 min with 20 ng/ml IGF-1. METTL1 was immunoprecipitated from 2.5 mg cell lysate protein using the antibody raised against mouse METTL1 residues 258–268, denatured in SDS, subjected to SDS–PAGE and, after transfer to Immobilon-P membranes, immunoblotted as in panel A.

    Article Snippet: Samples were denatured in SDS, subjected to SDS–PAGE, transferred to Immobilon P membranes and immunoblotted using the ECL detection system (Amersham Pharmacia Biotech).

    Techniques: Expressing, Incubation, Immunoprecipitation, SDS Page

    Effects of IGF1 and PMA on the phosphorylation of endogenous METTL1 in HEK293 cells. ( A ) Cells were serum starved for 8 h and then incubated for 20 min without (−) or with (+) 100 nM wortmannin before stimulation for a further 15 min with 20 ng/ml IGF-1 or for 30 min with 400 ng/ml PMA. METTL1 was immunoprecipitated from 1.5 mg cell lysate protein, denatured in SDS, subjected to SDS–PAGE and, after transfer to Immobilon-P membranes, immunoblotted with the antibody that recognises METTL1 phosphorylated at Ser27 and with the antibody that recognises phosphorylated and unphosphorylated METTL1. A further 20 μg of lysate protein was subjected to SDS–PAGE and immunoblotted with an antibody that recognises PKB phosphorylated at Thr308, an antibody that recognises phosphorylated and unphosphorylated PKBα equally well, an antibody that recognises ERK1 and ERK2 phosphorylated at the Thr-Glu-Tyr motif (pTEpY) and an antibody that recognises all forms of the ERK1 and ERK2 proteins. ( B ) Bacterially expressed human GST-METTL1 was left unphosphorylated (no kinase, NK) or maximally phosphorylated with PKBα or RSK2 and 100 ng aliquots spotted onto a nitrocellulose membrane and immunoblotted with the antibody that recognises METTL1 phosphorylated at Ser27 and with the antibody that recognises unphosphorylated and phosphorylated METTL1 equally well. ( C ) Same as panel A, except that after serum starvation for 8 h, the cells were incubated for 1 h in the absence or presence of 2 μM PD 184352 (instead of wortmannin) prior to stimulation with IGF-1 or PMA. ( D ) Same as panel A, except that following serum starvation the cells were incubated for 1 h in the absence or presence of 100 nM rapamycin (rather than wortmannin) prior to stimulation with IGF-1 or PMA. In addition, 20 μg cell lysate was immunoblotted with an antibody that recognises S6K1 phosphorylated at Thr389 and with an antibody that recognises phosphorylated and unphosphorylated S6K1 equally well.

    Journal: The EMBO Journal

    Article Title: The tRNA methylase METTL1 is phosphorylated and inactivated by PKB and RSK in vitro and in cells

    doi: 10.1038/sj.emboj.7600648

    Figure Lengend Snippet: Effects of IGF1 and PMA on the phosphorylation of endogenous METTL1 in HEK293 cells. ( A ) Cells were serum starved for 8 h and then incubated for 20 min without (−) or with (+) 100 nM wortmannin before stimulation for a further 15 min with 20 ng/ml IGF-1 or for 30 min with 400 ng/ml PMA. METTL1 was immunoprecipitated from 1.5 mg cell lysate protein, denatured in SDS, subjected to SDS–PAGE and, after transfer to Immobilon-P membranes, immunoblotted with the antibody that recognises METTL1 phosphorylated at Ser27 and with the antibody that recognises phosphorylated and unphosphorylated METTL1. A further 20 μg of lysate protein was subjected to SDS–PAGE and immunoblotted with an antibody that recognises PKB phosphorylated at Thr308, an antibody that recognises phosphorylated and unphosphorylated PKBα equally well, an antibody that recognises ERK1 and ERK2 phosphorylated at the Thr-Glu-Tyr motif (pTEpY) and an antibody that recognises all forms of the ERK1 and ERK2 proteins. ( B ) Bacterially expressed human GST-METTL1 was left unphosphorylated (no kinase, NK) or maximally phosphorylated with PKBα or RSK2 and 100 ng aliquots spotted onto a nitrocellulose membrane and immunoblotted with the antibody that recognises METTL1 phosphorylated at Ser27 and with the antibody that recognises unphosphorylated and phosphorylated METTL1 equally well. ( C ) Same as panel A, except that after serum starvation for 8 h, the cells were incubated for 1 h in the absence or presence of 2 μM PD 184352 (instead of wortmannin) prior to stimulation with IGF-1 or PMA. ( D ) Same as panel A, except that following serum starvation the cells were incubated for 1 h in the absence or presence of 100 nM rapamycin (rather than wortmannin) prior to stimulation with IGF-1 or PMA. In addition, 20 μg cell lysate was immunoblotted with an antibody that recognises S6K1 phosphorylated at Thr389 and with an antibody that recognises phosphorylated and unphosphorylated S6K1 equally well.

    Article Snippet: Samples were denatured in SDS, subjected to SDS–PAGE, transferred to Immobilon P membranes and immunoblotted using the ECL detection system (Amersham Pharmacia Biotech).

    Techniques: Incubation, Immunoprecipitation, SDS Page