putative pathogenicity related proteins Search Results


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    DSMZ c ureolyticus dsmz 20703
    Bioinformatics based predictions of the C. <t>ureolyticus</t> secretome. (A) 288 proteins were predicted to be secreted by C. ureolyticus <t>DSMZ</t> 20703 using the SignalP 4.1 server for classically secreted proteins and the SecretomeP 2.0 server for non-classically secreted proteins. Of the 288 proteins, 187 proteins were predicted to be secreted by the SecretomeP 2.0 sever alone, 57 proteins by the SignalP 4.1 alone and 44 proteins were identified by both servers. Additionally, 117 proteins of 28 were hypothetical, the remaining 171 proteins were associated with a diverse range of functions relating to membrane transport, protein metabolism, respiration, stress response and at least 25 proteins with putative virulence roles. (B) SignalP4.1 and SecretomeP2.0 servers predicted 269 proteins are secreted by C. ureolyticus ACS-301-V-Sch3b. 165 proteins were predicted to be secreted by the SecretomeP 2.0 server alone, a further 65 proteins were identified by the Signal 4.1 server and 39 proteins were identified by both servers. Of the 269 secreted proteins, a total of 94 were hypothetical proteins, the remaining 175 proteins were predicted to have a wide range of functions including DNA metabolism, cell wall synthesis, sulphur and nitrogen metabolism and at least 28 proteins with putative virulence roles.
    C Ureolyticus Dsmz 20703, supplied by DSMZ, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC a baumannii atcc 19606t
    Secretion of OMVs from A. <t>baumannii</t> during in vitro culture and in vivo infection. (A) Transmission electron micrograph of OMVs prepared from A. baumannii <t>ATCC</t> 19606 T cultured in LB broth for 24 h. (B and C) Secretion of OMVs from A. baumannii ATCC 19606 T in a murine pneumonia model. Mice were infected with 1×10 7 CFU of bacteria intratracheally and sacrificed 48 h after bacterial injection. Arrows indicate the OMVs secreted from A. baumannii .
    A Baumannii Atcc 19606t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC b cereus atcc 14579
    Secretion of OMVs from A. <t>baumannii</t> during in vitro culture and in vivo infection. (A) Transmission electron micrograph of OMVs prepared from A. baumannii <t>ATCC</t> 19606 T cultured in LB broth for 24 h. (B and C) Secretion of OMVs from A. baumannii ATCC 19606 T in a murine pneumonia model. Mice were infected with 1×10 7 CFU of bacteria intratracheally and sacrificed 48 h after bacterial injection. Arrows indicate the OMVs secreted from A. baumannii .
    B Cereus Atcc 14579, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Biotechnology Information biotechnology information ncbi
    Secretion of OMVs from A. <t>baumannii</t> during in vitro culture and in vivo infection. (A) Transmission electron micrograph of OMVs prepared from A. baumannii <t>ATCC</t> 19606 T cultured in LB broth for 24 h. (B and C) Secretion of OMVs from A. baumannii ATCC 19606 T in a murine pneumonia model. Mice were infected with 1×10 7 CFU of bacteria intratracheally and sacrificed 48 h after bacterial injection. Arrows indicate the OMVs secreted from A. baumannii .
    Biotechnology Information Ncbi, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Bioinformatics based predictions of the C. ureolyticus secretome. (A) 288 proteins were predicted to be secreted by C. ureolyticus DSMZ 20703 using the SignalP 4.1 server for classically secreted proteins and the SecretomeP 2.0 server for non-classically secreted proteins. Of the 288 proteins, 187 proteins were predicted to be secreted by the SecretomeP 2.0 sever alone, 57 proteins by the SignalP 4.1 alone and 44 proteins were identified by both servers. Additionally, 117 proteins of 28 were hypothetical, the remaining 171 proteins were associated with a diverse range of functions relating to membrane transport, protein metabolism, respiration, stress response and at least 25 proteins with putative virulence roles. (B) SignalP4.1 and SecretomeP2.0 servers predicted 269 proteins are secreted by C. ureolyticus ACS-301-V-Sch3b. 165 proteins were predicted to be secreted by the SecretomeP 2.0 server alone, a further 65 proteins were identified by the Signal 4.1 server and 39 proteins were identified by both servers. Of the 269 secreted proteins, a total of 94 were hypothetical proteins, the remaining 175 proteins were predicted to have a wide range of functions including DNA metabolism, cell wall synthesis, sulphur and nitrogen metabolism and at least 28 proteins with putative virulence roles.

    Journal: PLoS ONE

    Article Title: Genomic Investigation into Strain Heterogeneity and Pathogenic Potential of the Emerging Gastrointestinal Pathogen Campylobacter ureolyticus

    doi: 10.1371/journal.pone.0071515

    Figure Lengend Snippet: Bioinformatics based predictions of the C. ureolyticus secretome. (A) 288 proteins were predicted to be secreted by C. ureolyticus DSMZ 20703 using the SignalP 4.1 server for classically secreted proteins and the SecretomeP 2.0 server for non-classically secreted proteins. Of the 288 proteins, 187 proteins were predicted to be secreted by the SecretomeP 2.0 sever alone, 57 proteins by the SignalP 4.1 alone and 44 proteins were identified by both servers. Additionally, 117 proteins of 28 were hypothetical, the remaining 171 proteins were associated with a diverse range of functions relating to membrane transport, protein metabolism, respiration, stress response and at least 25 proteins with putative virulence roles. (B) SignalP4.1 and SecretomeP2.0 servers predicted 269 proteins are secreted by C. ureolyticus ACS-301-V-Sch3b. 165 proteins were predicted to be secreted by the SecretomeP 2.0 server alone, a further 65 proteins were identified by the Signal 4.1 server and 39 proteins were identified by both servers. Of the 269 secreted proteins, a total of 94 were hypothetical proteins, the remaining 175 proteins were predicted to have a wide range of functions including DNA metabolism, cell wall synthesis, sulphur and nitrogen metabolism and at least 28 proteins with putative virulence roles.

    Article Snippet: Virulence determinants conserved between C. ureolyticus DSMZ 20703 and ACS-301-V-Sch3b Amongst the 1,469 homologous proteins ( and ) shared between these two strains include a minimum of 35 putative virulence factors ( and ) associated with motility and biofilm formation, adhesion and invasion.

    Techniques:

    Secretion of OMVs from A. baumannii during in vitro culture and in vivo infection. (A) Transmission electron micrograph of OMVs prepared from A. baumannii ATCC 19606 T cultured in LB broth for 24 h. (B and C) Secretion of OMVs from A. baumannii ATCC 19606 T in a murine pneumonia model. Mice were infected with 1×10 7 CFU of bacteria intratracheally and sacrificed 48 h after bacterial injection. Arrows indicate the OMVs secreted from A. baumannii .

    Journal: PLoS ONE

    Article Title: Acinetobacter baumannii Secretes Cytotoxic Outer Membrane Protein A via Outer Membrane Vesicles

    doi: 10.1371/journal.pone.0017027

    Figure Lengend Snippet: Secretion of OMVs from A. baumannii during in vitro culture and in vivo infection. (A) Transmission electron micrograph of OMVs prepared from A. baumannii ATCC 19606 T cultured in LB broth for 24 h. (B and C) Secretion of OMVs from A. baumannii ATCC 19606 T in a murine pneumonia model. Mice were infected with 1×10 7 CFU of bacteria intratracheally and sacrificed 48 h after bacterial injection. Arrows indicate the OMVs secreted from A. baumannii .

    Article Snippet: Virulence-associated proteins, including AbOmpA, CsuA/B (A1S_2218), CsuC (A1S_2215), CsuD (A1S_2214), putative hemolysin (A1S_1321), putative serine protease (A1S_2525), Cu/Zn superoxide dismutase (A1S_3143), fimbrial protein (A1S_1510), bacterioferritin (A1S_3175), RND superfamily transporter (A1S_0116), putative RND type efflux pump (A1S_0009), and putative protease (A1S_2470), were found in the OMVs of A. baumannii ATCC 19606T .

    Techniques: In Vitro, In Vivo, Infection, Transmission Assay, Cell Culture, Mouse Assay, Injection

    Flow cytometric analysis of cell death induced by the OMVs from A. baumannii ATCC 19606 T and the Δ ompA mutant. The differentiated U937 cells were treated with various concentrations (0, 10, 20, 50, and 100 µg/ml) of OMVs and stained with Annexin V and PI. Upper panel, control cells without OMVs for 24 h. Middle panel, the cells were treated with OMVs from A. baumannii ATCC 19606 T for 24 h. Lower panel, the cells were treated with OMVs from the Δ ompA mutant for 24 h. Representative data from three independent experiments are shown. In the graph, cells in right upper and lower parts are apoptotic cells and cells in left upper part are necrotic cells.

    Journal: PLoS ONE

    Article Title: Acinetobacter baumannii Secretes Cytotoxic Outer Membrane Protein A via Outer Membrane Vesicles

    doi: 10.1371/journal.pone.0017027

    Figure Lengend Snippet: Flow cytometric analysis of cell death induced by the OMVs from A. baumannii ATCC 19606 T and the Δ ompA mutant. The differentiated U937 cells were treated with various concentrations (0, 10, 20, 50, and 100 µg/ml) of OMVs and stained with Annexin V and PI. Upper panel, control cells without OMVs for 24 h. Middle panel, the cells were treated with OMVs from A. baumannii ATCC 19606 T for 24 h. Lower panel, the cells were treated with OMVs from the Δ ompA mutant for 24 h. Representative data from three independent experiments are shown. In the graph, cells in right upper and lower parts are apoptotic cells and cells in left upper part are necrotic cells.

    Article Snippet: Virulence-associated proteins, including AbOmpA, CsuA/B (A1S_2218), CsuC (A1S_2215), CsuD (A1S_2214), putative hemolysin (A1S_1321), putative serine protease (A1S_2525), Cu/Zn superoxide dismutase (A1S_3143), fimbrial protein (A1S_1510), bacterioferritin (A1S_3175), RND superfamily transporter (A1S_0116), putative RND type efflux pump (A1S_0009), and putative protease (A1S_2470), were found in the OMVs of A. baumannii ATCC 19606T .

    Techniques: Flow Cytometry, Mutagenesis, Staining

    A. baumannii OMVs contain biologically active proteins. (A) A. baumannii OMVs were incubated with 100 mM nitrocefin in PBS at 30°C for 30 min. Hydrolysis of nitrocefin was monitored at 480 nm using a UV spectrophotometer. Inlet figure: left, brown colorization of nitrocefin in A. baumannii OMVs; right, negative control of nitrocefin in PBS. (B) SDS-PAGE of proteins packaged in the OMVs from A. baumannii ATCC 19606 T (lanes 1 and 2) and its Western blot analysis (lanes 3 and 4). The samples were immunoblotted with a rabbit anti-AbOmpA immune serum. Lanes 1 and 3, molecular weight maker; 2 and 4, OMV fraction. Arrows indicate AbOmpA.

    Journal: PLoS ONE

    Article Title: Acinetobacter baumannii Secretes Cytotoxic Outer Membrane Protein A via Outer Membrane Vesicles

    doi: 10.1371/journal.pone.0017027

    Figure Lengend Snippet: A. baumannii OMVs contain biologically active proteins. (A) A. baumannii OMVs were incubated with 100 mM nitrocefin in PBS at 30°C for 30 min. Hydrolysis of nitrocefin was monitored at 480 nm using a UV spectrophotometer. Inlet figure: left, brown colorization of nitrocefin in A. baumannii OMVs; right, negative control of nitrocefin in PBS. (B) SDS-PAGE of proteins packaged in the OMVs from A. baumannii ATCC 19606 T (lanes 1 and 2) and its Western blot analysis (lanes 3 and 4). The samples were immunoblotted with a rabbit anti-AbOmpA immune serum. Lanes 1 and 3, molecular weight maker; 2 and 4, OMV fraction. Arrows indicate AbOmpA.

    Article Snippet: Virulence-associated proteins, including AbOmpA, CsuA/B (A1S_2218), CsuC (A1S_2215), CsuD (A1S_2214), putative hemolysin (A1S_1321), putative serine protease (A1S_2525), Cu/Zn superoxide dismutase (A1S_3143), fimbrial protein (A1S_1510), bacterioferritin (A1S_3175), RND superfamily transporter (A1S_0116), putative RND type efflux pump (A1S_0009), and putative protease (A1S_2470), were found in the OMVs of A. baumannii ATCC 19606T .

    Techniques: Incubation, Spectrophotometry, Negative Control, SDS Page, Western Blot, Molecular Weight