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Image Search Results

Journal: The European Journal of Neuroscience
Article Title: Brain‐derived neurotrophic factor overexpression in taste buds diminishes chemotherapy induced taste loss
doi: 10.1111/ejn.15799
Figure Lengend Snippet: BDNF overexpression protects P2X3 labelling of taste buds and gustatory innervation. IHC with P2X3 antibodies showed preserved innervation inside the taste buds (d, thick arrow) and in the subepithelial nerve plexus of the circumvallate papillae of vismodegib‐treated GB759 (d, thin arrow) compared to vismodegib‐treated wild‐type mice (c). Both vismodegib‐treated (d) and vehicle‐treated (b) GB759 mice showed stronger labeling compared to their wild‐type controls (a,c) Because of the orientation of the section, the subepithelial nerve plexus is not visible in vehicle‐treated GB759mice (b). There was a significant decrease in P2X3 fluorescence intensity in vismodegib‐treated wild‐type mice (e), whereas there was no significant decrease in vismodegib‐treated GB759 mice. Scale bar = 50 µm. * p < 0.05; *** p < 0.001. IHC, immunohistochemistry
Article Snippet: Immunohistochemical detection of Sonic Hedgehog (Shh, rabbit, Santa Cruz, 1:100), Ki67 (rat, eBioscience, 1:100), Taste 1 receptor member 3 (T1R3, goat, Santa Cruz Biotechnology, 1:500), α‐Gustducin (rabbit, Santa‐Cruz, 1:200), Troma‐1 (rat, Hybridoma bank, 1:80), neural cell adhesion molecule (NCAM, rabbit, Chemicon, 1:400) and
Techniques: Over Expression, Labeling, Fluorescence, Immunohistochemistry