puc19 Stratagene Search Results


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  • 92
    Stratagene puc19
    (GAA·TTC) n constructs used to analyze repeat instability. (GAA·TTC) n sequences of the indicated lengths were cloned into the Pst I/Xba I sites of <t>pUC19</t> in both orientations relative to the unidirectional pMB1 origin of replication. Repeat-containing plasmids are depicted in either the ‘GAA’ or ‘TTC’ orientations, based on whether (GAA) n or (TTC) n serves as the lagging strand template, respectively. The plasmid constructs contain repeat lengths of n = 21, 41 and 79. The black boxes flanking the repeat represent minimal flanking sequence from intron 1 of the FXN gene.
    Puc19, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/puc19/product/Stratagene
    Average 92 stars, based on 237 article reviews
    Price from $9.99 to $1999.99
    puc19 - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    90
    Stratagene plasmid puc19
    Amplification of <t>pUC19</t> from colonies and M13mp19 from plaques. Amplification reactions were performed as indicated directly on material picked from a colony or a plaque. Half of the radioactively labeled reaction products were cleaved with Eco RI and both cleaved and uncleaved samples were analyzed by agarose gel electrophoresis. The positions of linear, duplex M13, and pUC19 DNA are indicated.
    Plasmid Puc19, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid puc19/product/Stratagene
    Average 90 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    plasmid puc19 - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    90
    Stratagene puc19 pid2
    Amplification of <t>pUC19</t> from colonies and M13mp19 from plaques. Amplification reactions were performed as indicated directly on material picked from a colony or a plaque. Half of the radioactively labeled reaction products were cleaved with Eco RI and both cleaved and uncleaved samples were analyzed by agarose gel electrophoresis. The positions of linear, duplex M13, and pUC19 DNA are indicated.
    Puc19 Pid2, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/puc19 pid2/product/Stratagene
    Average 90 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    puc19 pid2 - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    92
    Stratagene puc19 derived pbluescript sk vector
    Amplification of <t>pUC19</t> from colonies and M13mp19 from plaques. Amplification reactions were performed as indicated directly on material picked from a colony or a plaque. Half of the radioactively labeled reaction products were cleaved with Eco RI and both cleaved and uncleaved samples were analyzed by agarose gel electrophoresis. The positions of linear, duplex M13, and pUC19 DNA are indicated.
    Puc19 Derived Pbluescript Sk Vector, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/puc19 derived pbluescript sk vector/product/Stratagene
    Average 92 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    puc19 derived pbluescript sk vector - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    99
    Stratagene quikchange mutagenesis kit
    Amplification of <t>pUC19</t> from colonies and M13mp19 from plaques. Amplification reactions were performed as indicated directly on material picked from a colony or a plaque. Half of the radioactively labeled reaction products were cleaved with Eco RI and both cleaved and uncleaved samples were analyzed by agarose gel electrophoresis. The positions of linear, duplex M13, and pUC19 DNA are indicated.
    Quikchange Mutagenesis Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 99/100, based on 11351 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quikchange mutagenesis kit/product/Stratagene
    Average 99 stars, based on 11351 article reviews
    Price from $9.99 to $1999.99
    quikchange mutagenesis kit - by Bioz Stars, 2020-05
    99/100 stars
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    Image Search Results


    (GAA·TTC) n constructs used to analyze repeat instability. (GAA·TTC) n sequences of the indicated lengths were cloned into the Pst I/Xba I sites of pUC19 in both orientations relative to the unidirectional pMB1 origin of replication. Repeat-containing plasmids are depicted in either the ‘GAA’ or ‘TTC’ orientations, based on whether (GAA) n or (TTC) n serves as the lagging strand template, respectively. The plasmid constructs contain repeat lengths of n = 21, 41 and 79. The black boxes flanking the repeat represent minimal flanking sequence from intron 1 of the FXN gene.

    Journal: Nucleic Acids Research

    Article Title: Deficiency of RecA-dependent RecFOR and RecBCD pathways causes increased instability of the (GAA?TTC)n sequence when GAA is the lagging strand template

    doi: 10.1093/nar/gkm810

    Figure Lengend Snippet: (GAA·TTC) n constructs used to analyze repeat instability. (GAA·TTC) n sequences of the indicated lengths were cloned into the Pst I/Xba I sites of pUC19 in both orientations relative to the unidirectional pMB1 origin of replication. Repeat-containing plasmids are depicted in either the ‘GAA’ or ‘TTC’ orientations, based on whether (GAA) n or (TTC) n serves as the lagging strand template, respectively. The plasmid constructs contain repeat lengths of n = 21, 41 and 79. The black boxes flanking the repeat represent minimal flanking sequence from intron 1 of the FXN gene.

    Article Snippet: Deletion of the Plac promoter in pUC19, to produce the pDEL-GAA-79 construct ( A), was accomplished by first introducing an Apa I site at the −35 position using the QuikChange II XL site-directed mutagenesis kit (Stratagene), followed by removal of the fragment between Apa I and Hind III, thus deleting both the −10 and −35 sites.

    Techniques: Construct, Clone Assay, Plasmid Preparation, Sequencing

    RecA deficiency causes increased instability when GAA is the lagging strand template irrespective of transcription through the repeat tract, or the distance between origin of replication and the (GAA·TTC) 79 sequence. (A) The (GAA·TTC) 79 sequence was additionally subcloned in the GAA orientation into the pDEL (with a deletion of the Plac promoter in pUC19) and pINS (with a 1.5 kb spacer from intron 1 of the human FXN gene inserted in pUC19 between the origin of replication and the repeat tract) vectors (see Materials and Methods section for details). (B) Instability of the (GAA·TTC) 79 sequence was significantly enhanced in the M152 (RecA-deficient) versus MM28 (RecA-proficient) strain. Error bars depict +/− 2SEM; ** P

    Journal: Nucleic Acids Research

    Article Title: Deficiency of RecA-dependent RecFOR and RecBCD pathways causes increased instability of the (GAA?TTC)n sequence when GAA is the lagging strand template

    doi: 10.1093/nar/gkm810

    Figure Lengend Snippet: RecA deficiency causes increased instability when GAA is the lagging strand template irrespective of transcription through the repeat tract, or the distance between origin of replication and the (GAA·TTC) 79 sequence. (A) The (GAA·TTC) 79 sequence was additionally subcloned in the GAA orientation into the pDEL (with a deletion of the Plac promoter in pUC19) and pINS (with a 1.5 kb spacer from intron 1 of the human FXN gene inserted in pUC19 between the origin of replication and the repeat tract) vectors (see Materials and Methods section for details). (B) Instability of the (GAA·TTC) 79 sequence was significantly enhanced in the M152 (RecA-deficient) versus MM28 (RecA-proficient) strain. Error bars depict +/− 2SEM; ** P

    Article Snippet: Deletion of the Plac promoter in pUC19, to produce the pDEL-GAA-79 construct ( A), was accomplished by first introducing an Apa I site at the −35 position using the QuikChange II XL site-directed mutagenesis kit (Stratagene), followed by removal of the fragment between Apa I and Hind III, thus deleting both the −10 and −35 sites.

    Techniques: Sequencing

    Amplification of pUC19 from colonies and M13mp19 from plaques. Amplification reactions were performed as indicated directly on material picked from a colony or a plaque. Half of the radioactively labeled reaction products were cleaved with Eco RI and both cleaved and uncleaved samples were analyzed by agarose gel electrophoresis. The positions of linear, duplex M13, and pUC19 DNA are indicated.

    Journal: Genome Research

    Article Title: Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification

    doi: 10.1101/gr.180501

    Figure Lengend Snippet: Amplification of pUC19 from colonies and M13mp19 from plaques. Amplification reactions were performed as indicated directly on material picked from a colony or a plaque. Half of the radioactively labeled reaction products were cleaved with Eco RI and both cleaved and uncleaved samples were analyzed by agarose gel electrophoresis. The positions of linear, duplex M13, and pUC19 DNA are indicated.

    Article Snippet: Polyethylene tubing (Intramedic, PE20, 1.09-mm outer diameter) 1 cm in length was stabbed into a colony of Escherichia coli transformed with plasmid pUC19 or a plaque of bacteriophage M13mp19 in a lawn of E. coli (XL1-blue, Stratagene).

    Techniques: Amplification, Labeling, Agarose Gel Electrophoresis