Article Title: Construction and Properties of a Herpes Simplex Virus 2 dl5-29 Vaccine Candidate Strain Encoding an HSV-1 Virion Host Shutoff Protein
Figure Lengend Snippet: Schematic diagrams of the HSV and recombinant viral genomes (A) Genome of wild-type HSV. Shaded boxes represent repeated sequences, and lines represent unique sequences. (B) Genome of the dl 5-29-41L triple mutant virus. The U L 5 and U L 29 genes contain deletions, and the U L 41 gene contains an insertion of a lac Z expression cassette. (C) An expanded view of the U L 41 gene disrupted by the lac Z expression cassette in dl 5-29-41L. (D) The HSV-1 KOS DNA fragment containing the U L 41 gene and flanking regions introduced into plasmid pUC19 to generate the pUC41.1 plasmid. Dashed lines define the boundaries of the homologous sequences in which recombination may have occurred to generate dl 5-29-41.1. Nucleotide numbers in (C) correspond to the HSV-2 HG-52 viral genome and in (D) to the HSV-1 strain 17 viral genome.
Article Snippet: A 3585 bp HindIII-HpaI DNA fragment containing the HSV-1 UL 41 gene and flanking sequences was isolated from the pSG124 plasmid [ ] and cloned into the Hind III-Sma I sites of plasmid pUC19 (Promega) to generate the pUC41.1 plasmid.
Techniques: Recombinant, Mutagenesis, Expressing, Plasmid Preparation