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  • 92
    GE Healthcare puc19
    Analysis of the I- Uma I target site specificity. CF from induced pAP2 cells was incubated with various substrate plasmids under standard conditions for analysis of cleavage efficiencies (see Materials and Methods). Letters denote the individual substrate plasmids ( e.g. D for <t>pUC19-D).</t> Reaction products were additionally cleaved with Ssp I to yield 2.1 and 0.63 kb fragments in case of cleavage (schematic on the right: the I- Uma I target site fragment is indicated as black box. Xba I cleaves at the right border and in combination with Ssp I produces fragments similar in size to those produced by I- Uma I/ Ssp I). Marker lanes: lin. S, pUC19-D cleaved with Ssp I; lin. X/S, pUC19-D cleaved with Xba I/ Ssp I; s.c./circ., uncleaved pUC19-D showing the supercoiled and circular forms. The + and - symbols refer to the cleavage efficiencies. +++, complete cleavage; ++/+++, > 50% cleavage; ++, ∼50% cleavage; +,
    Puc19, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare puc19 pharmacia amersham
    Analysis of the I- Uma I target site specificity. CF from induced pAP2 cells was incubated with various substrate plasmids under standard conditions for analysis of cleavage efficiencies (see Materials and Methods). Letters denote the individual substrate plasmids ( e.g. D for <t>pUC19-D).</t> Reaction products were additionally cleaved with Ssp I to yield 2.1 and 0.63 kb fragments in case of cleavage (schematic on the right: the I- Uma I target site fragment is indicated as black box. Xba I cleaves at the right border and in combination with Ssp I produces fragments similar in size to those produced by I- Uma I/ Ssp I). Marker lanes: lin. S, pUC19-D cleaved with Ssp I; lin. X/S, pUC19-D cleaved with Xba I/ Ssp I; s.c./circ., uncleaved pUC19-D showing the supercoiled and circular forms. The + and - symbols refer to the cleavage efficiencies. +++, complete cleavage; ++/+++, > 50% cleavage; ++, ∼50% cleavage; +,
    Puc19 Pharmacia Amersham, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GE Healthcare plasmid vector puc19
    Analysis of the I- Uma I target site specificity. CF from induced pAP2 cells was incubated with various substrate plasmids under standard conditions for analysis of cleavage efficiencies (see Materials and Methods). Letters denote the individual substrate plasmids ( e.g. D for <t>pUC19-D).</t> Reaction products were additionally cleaved with Ssp I to yield 2.1 and 0.63 kb fragments in case of cleavage (schematic on the right: the I- Uma I target site fragment is indicated as black box. Xba I cleaves at the right border and in combination with Ssp I produces fragments similar in size to those produced by I- Uma I/ Ssp I). Marker lanes: lin. S, pUC19-D cleaved with Ssp I; lin. X/S, pUC19-D cleaved with Xba I/ Ssp I; s.c./circ., uncleaved pUC19-D showing the supercoiled and circular forms. The + and - symbols refer to the cleavage efficiencies. +++, complete cleavage; ++/+++, > 50% cleavage; ++, ∼50% cleavage; +,
    Plasmid Vector Puc19, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GE Healthcare sureclone ligation kit
    Analysis of the I- Uma I target site specificity. CF from induced pAP2 cells was incubated with various substrate plasmids under standard conditions for analysis of cleavage efficiencies (see Materials and Methods). Letters denote the individual substrate plasmids ( e.g. D for <t>pUC19-D).</t> Reaction products were additionally cleaved with Ssp I to yield 2.1 and 0.63 kb fragments in case of cleavage (schematic on the right: the I- Uma I target site fragment is indicated as black box. Xba I cleaves at the right border and in combination with Ssp I produces fragments similar in size to those produced by I- Uma I/ Ssp I). Marker lanes: lin. S, pUC19-D cleaved with Ssp I; lin. X/S, pUC19-D cleaved with Xba I/ Ssp I; s.c./circ., uncleaved pUC19-D showing the supercoiled and circular forms. The + and - symbols refer to the cleavage efficiencies. +++, complete cleavage; ++/+++, > 50% cleavage; ++, ∼50% cleavage; +,
    Sureclone Ligation Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    GE Healthcare labstation sequencing kit
    Analysis of the I- Uma I target site specificity. CF from induced pAP2 cells was incubated with various substrate plasmids under standard conditions for analysis of cleavage efficiencies (see Materials and Methods). Letters denote the individual substrate plasmids ( e.g. D for <t>pUC19-D).</t> Reaction products were additionally cleaved with Ssp I to yield 2.1 and 0.63 kb fragments in case of cleavage (schematic on the right: the I- Uma I target site fragment is indicated as black box. Xba I cleaves at the right border and in combination with Ssp I produces fragments similar in size to those produced by I- Uma I/ Ssp I). Marker lanes: lin. S, pUC19-D cleaved with Ssp I; lin. X/S, pUC19-D cleaved with Xba I/ Ssp I; s.c./circ., uncleaved pUC19-D showing the supercoiled and circular forms. The + and - symbols refer to the cleavage efficiencies. +++, complete cleavage; ++/+++, > 50% cleavage; ++, ∼50% cleavage; +,
    Labstation Sequencing Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 80/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/labstation sequencing kit/product/GE Healthcare
    Average 80 stars, based on 8 article reviews
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    labstation sequencing kit - by Bioz Stars, 2020-05
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    Image Search Results


    Analysis of the I- Uma I target site specificity. CF from induced pAP2 cells was incubated with various substrate plasmids under standard conditions for analysis of cleavage efficiencies (see Materials and Methods). Letters denote the individual substrate plasmids ( e.g. D for pUC19-D). Reaction products were additionally cleaved with Ssp I to yield 2.1 and 0.63 kb fragments in case of cleavage (schematic on the right: the I- Uma I target site fragment is indicated as black box. Xba I cleaves at the right border and in combination with Ssp I produces fragments similar in size to those produced by I- Uma I/ Ssp I). Marker lanes: lin. S, pUC19-D cleaved with Ssp I; lin. X/S, pUC19-D cleaved with Xba I/ Ssp I; s.c./circ., uncleaved pUC19-D showing the supercoiled and circular forms. The + and - symbols refer to the cleavage efficiencies. +++, complete cleavage; ++/+++, > 50% cleavage; ++, ∼50% cleavage; +,

    Journal: PLoS ONE

    Article Title: The Mitochondrial LSU rRNA Group II Intron of Ustilago maydis Encodes an Active Homing Endonuclease Likely Involved in Intron Mobility

    doi: 10.1371/journal.pone.0049551

    Figure Lengend Snippet: Analysis of the I- Uma I target site specificity. CF from induced pAP2 cells was incubated with various substrate plasmids under standard conditions for analysis of cleavage efficiencies (see Materials and Methods). Letters denote the individual substrate plasmids ( e.g. D for pUC19-D). Reaction products were additionally cleaved with Ssp I to yield 2.1 and 0.63 kb fragments in case of cleavage (schematic on the right: the I- Uma I target site fragment is indicated as black box. Xba I cleaves at the right border and in combination with Ssp I produces fragments similar in size to those produced by I- Uma I/ Ssp I). Marker lanes: lin. S, pUC19-D cleaved with Ssp I; lin. X/S, pUC19-D cleaved with Xba I/ Ssp I; s.c./circ., uncleaved pUC19-D showing the supercoiled and circular forms. The + and - symbols refer to the cleavage efficiencies. +++, complete cleavage; ++/+++, > 50% cleavage; ++, ∼50% cleavage; +,

    Article Snippet: Blunt-ended plasmids were religated either in the absence or presence of the 692 bp Dra I fragment isolated from pUC19.

    Techniques: Incubation, Produced, Marker