ptripz vector expressing shrna against ampkα2 Search Results


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  • 99
    Thermo Fisher lipofectamine
    Lipofectamine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 61553 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lipofectamine/product/Thermo Fisher
    Average 99 stars, based on 61553 article reviews
    Price from $9.99 to $1999.99
    lipofectamine - by Bioz Stars, 2020-05
    99/100 stars
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    92
    Horizon Discovery control nontargeting shrna
    AMPK promotes Akt dephosphorylation in suspension via PHLPP. A and B, Representative immunoblots of MDA-MB-231 cells treated with DMSO or compound C (CC) and subjected to suspension (Sus) for 8 hours ( n = 5; A ), and cells grown in adherent (Att) or suspension (Sus) condition for 10 minutes and 8 hours ( n = 3; B ). C, Phosphatase assay performed with immunoprecipitated PHLPP2; IgG was used as control; n = 4. Error bars, mean ± SEM. D–G, Representative immunoblots of MDA-MB-231 cells harvested under conditions detailed below. D, Cells stably expressing <t>nontargeting</t> <t>shRNA</t> (NT) or shPHLPP2 (seq #5) and subjected to suspension (Sus) for 8 hours; n = 4. E, Cells treated with DMSO or compound C (CC) were subjected to suspension (Sus) for 8 hours; n = 3. F, Adherent cells stably expressing pTRIPZ empty vector (EV) or shAMPKα2 (seq #1) were induced with doxycycline for 48 hours, followed by suspension for 8 hours; n = 3. G, Adherent cells pretreated with DMSO or AMPK inhibitor (CC) were treated with cycloheximide (CHX) for 20 minutes, followed by suspension culture (Sus) for indicated time points; n = 3. Graph represents quantification of PHLPP2. All values represent densitometric analyses of Western blots to quantify relative levels of specified proteins.
    Control Nontargeting Shrna, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control nontargeting shrna/product/Horizon Discovery
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    control nontargeting shrna - by Bioz Stars, 2020-05
    92/100 stars
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    92
    Horizon Discovery control empty ptripz vector ev
    AMPK promotes Akt dephosphorylation in suspension via PHLPP. A and B, Representative immunoblots of MDA-MB-231 cells treated with DMSO or compound C (CC) and subjected to suspension (Sus) for 8 hours ( n = 5; A ), and cells grown in adherent (Att) or suspension (Sus) condition for 10 minutes and 8 hours ( n = 3; B ). C, Phosphatase assay performed with immunoprecipitated PHLPP2; IgG was used as control; n = 4. Error bars, mean ± SEM. D–G, Representative immunoblots of MDA-MB-231 cells harvested under conditions detailed below. D, Cells stably expressing nontargeting <t>shRNA</t> (NT) or shPHLPP2 (seq #5) and subjected to suspension (Sus) for 8 hours; n = 4. E, Cells treated with DMSO or compound C (CC) were subjected to suspension (Sus) for 8 hours; n = 3. F, Adherent cells stably expressing <t>pTRIPZ</t> empty vector (EV) or shAMPKα2 (seq #1) were induced with doxycycline for 48 hours, followed by suspension for 8 hours; n = 3. G, Adherent cells pretreated with DMSO or AMPK inhibitor (CC) were treated with cycloheximide (CHX) for 20 minutes, followed by suspension culture (Sus) for indicated time points; n = 3. Graph represents quantification of PHLPP2. All values represent densitometric analyses of Western blots to quantify relative levels of specified proteins.
    Control Empty Ptripz Vector Ev, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control empty ptripz vector ev/product/Horizon Discovery
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    control empty ptripz vector ev - by Bioz Stars, 2020-05
    92/100 stars
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    Image Search Results


    AMPK promotes Akt dephosphorylation in suspension via PHLPP. A and B, Representative immunoblots of MDA-MB-231 cells treated with DMSO or compound C (CC) and subjected to suspension (Sus) for 8 hours ( n = 5; A ), and cells grown in adherent (Att) or suspension (Sus) condition for 10 minutes and 8 hours ( n = 3; B ). C, Phosphatase assay performed with immunoprecipitated PHLPP2; IgG was used as control; n = 4. Error bars, mean ± SEM. D–G, Representative immunoblots of MDA-MB-231 cells harvested under conditions detailed below. D, Cells stably expressing nontargeting shRNA (NT) or shPHLPP2 (seq #5) and subjected to suspension (Sus) for 8 hours; n = 4. E, Cells treated with DMSO or compound C (CC) were subjected to suspension (Sus) for 8 hours; n = 3. F, Adherent cells stably expressing pTRIPZ empty vector (EV) or shAMPKα2 (seq #1) were induced with doxycycline for 48 hours, followed by suspension for 8 hours; n = 3. G, Adherent cells pretreated with DMSO or AMPK inhibitor (CC) were treated with cycloheximide (CHX) for 20 minutes, followed by suspension culture (Sus) for indicated time points; n = 3. Graph represents quantification of PHLPP2. All values represent densitometric analyses of Western blots to quantify relative levels of specified proteins.

    Journal: Cancer research

    Article Title: AMPK–Akt Double-Negative Feedback Loop in Breast Cancer Cells Regulates Their Adaptation to Matrix Deprivation

    doi: 10.1158/0008-5472.CAN-17-2090

    Figure Lengend Snippet: AMPK promotes Akt dephosphorylation in suspension via PHLPP. A and B, Representative immunoblots of MDA-MB-231 cells treated with DMSO or compound C (CC) and subjected to suspension (Sus) for 8 hours ( n = 5; A ), and cells grown in adherent (Att) or suspension (Sus) condition for 10 minutes and 8 hours ( n = 3; B ). C, Phosphatase assay performed with immunoprecipitated PHLPP2; IgG was used as control; n = 4. Error bars, mean ± SEM. D–G, Representative immunoblots of MDA-MB-231 cells harvested under conditions detailed below. D, Cells stably expressing nontargeting shRNA (NT) or shPHLPP2 (seq #5) and subjected to suspension (Sus) for 8 hours; n = 4. E, Cells treated with DMSO or compound C (CC) were subjected to suspension (Sus) for 8 hours; n = 3. F, Adherent cells stably expressing pTRIPZ empty vector (EV) or shAMPKα2 (seq #1) were induced with doxycycline for 48 hours, followed by suspension for 8 hours; n = 3. G, Adherent cells pretreated with DMSO or AMPK inhibitor (CC) were treated with cycloheximide (CHX) for 20 minutes, followed by suspension culture (Sus) for indicated time points; n = 3. Graph represents quantification of PHLPP2. All values represent densitometric analyses of Western blots to quantify relative levels of specified proteins.

    Article Snippet: HA myr-Akt and GFP CA CaMKK were provided by Dr. Joseph Testa and Dr. Grahame D. Hardie, respectively, as kind gifts. shRNAs against PHLPP2 (RHS4531-EG23035) and the corresponding control nontargeting shRNA in pGIPZ vector (NT); and inducible shRNA against AMPKα2 (V2THS_57674) and the corresponding control empty pTRIPZ vector (EV) were procured from Dharmacon.

    Techniques: De-Phosphorylation Assay, Western Blot, Multiple Displacement Amplification, Phosphatase Assay, Immunoprecipitation, Stable Transfection, Expressing, shRNA, Plasmid Preparation

    AMPK promotes Akt dephosphorylation in suspension via PHLPP. A and B, Representative immunoblots of MDA-MB-231 cells treated with DMSO or compound C (CC) and subjected to suspension (Sus) for 8 hours ( n = 5; A ), and cells grown in adherent (Att) or suspension (Sus) condition for 10 minutes and 8 hours ( n = 3; B ). C, Phosphatase assay performed with immunoprecipitated PHLPP2; IgG was used as control; n = 4. Error bars, mean ± SEM. D–G, Representative immunoblots of MDA-MB-231 cells harvested under conditions detailed below. D, Cells stably expressing nontargeting shRNA (NT) or shPHLPP2 (seq #5) and subjected to suspension (Sus) for 8 hours; n = 4. E, Cells treated with DMSO or compound C (CC) were subjected to suspension (Sus) for 8 hours; n = 3. F, Adherent cells stably expressing pTRIPZ empty vector (EV) or shAMPKα2 (seq #1) were induced with doxycycline for 48 hours, followed by suspension for 8 hours; n = 3. G, Adherent cells pretreated with DMSO or AMPK inhibitor (CC) were treated with cycloheximide (CHX) for 20 minutes, followed by suspension culture (Sus) for indicated time points; n = 3. Graph represents quantification of PHLPP2. All values represent densitometric analyses of Western blots to quantify relative levels of specified proteins.

    Journal: Cancer research

    Article Title: AMPK–Akt Double-Negative Feedback Loop in Breast Cancer Cells Regulates Their Adaptation to Matrix Deprivation

    doi: 10.1158/0008-5472.CAN-17-2090

    Figure Lengend Snippet: AMPK promotes Akt dephosphorylation in suspension via PHLPP. A and B, Representative immunoblots of MDA-MB-231 cells treated with DMSO or compound C (CC) and subjected to suspension (Sus) for 8 hours ( n = 5; A ), and cells grown in adherent (Att) or suspension (Sus) condition for 10 minutes and 8 hours ( n = 3; B ). C, Phosphatase assay performed with immunoprecipitated PHLPP2; IgG was used as control; n = 4. Error bars, mean ± SEM. D–G, Representative immunoblots of MDA-MB-231 cells harvested under conditions detailed below. D, Cells stably expressing nontargeting shRNA (NT) or shPHLPP2 (seq #5) and subjected to suspension (Sus) for 8 hours; n = 4. E, Cells treated with DMSO or compound C (CC) were subjected to suspension (Sus) for 8 hours; n = 3. F, Adherent cells stably expressing pTRIPZ empty vector (EV) or shAMPKα2 (seq #1) were induced with doxycycline for 48 hours, followed by suspension for 8 hours; n = 3. G, Adherent cells pretreated with DMSO or AMPK inhibitor (CC) were treated with cycloheximide (CHX) for 20 minutes, followed by suspension culture (Sus) for indicated time points; n = 3. Graph represents quantification of PHLPP2. All values represent densitometric analyses of Western blots to quantify relative levels of specified proteins.

    Article Snippet: HA myr-Akt and GFP CA CaMKK were provided by Dr. Joseph Testa and Dr. Grahame D. Hardie, respectively, as kind gifts. shRNAs against PHLPP2 (RHS4531-EG23035) and the corresponding control nontargeting shRNA in pGIPZ vector (NT); and inducible shRNA against AMPKα2 (V2THS_57674) and the corresponding control empty pTRIPZ vector (EV) were procured from Dharmacon.

    Techniques: De-Phosphorylation Assay, Western Blot, Multiple Displacement Amplification, Phosphatase Assay, Immunoprecipitation, Stable Transfection, Expressing, shRNA, Plasmid Preparation