ptripz vector Search Results


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  • 93
    Thermo Fisher ptripz vector
    Dose-dependent effect of E2F1 on DNA synthesis and content. Cells expressing <t>pTRIPZ-YFP-ER-E2F1</t> were starved of growth factors for 2 days and then treated with a combination of 1 μ g/ml doxycycline, 90 nM OHT or control starvation medium in the presence of 10 μ M EdU. Cells were fixed and stained for DNA content (DAPI) and DNA synthesis (EdU incorporation) 20 h later. ( a ) YFP/EdU distribution from induced HME cells. Red horizontal dashed line represents the boundary between EdU-positive and EdU-negative cells. Regions of low, intermediate and high YFP used for plotting DNA/EdU distributions in ( c ) are marked with red boxes. ( b ) YFP/DNA distribution from induced HME cells. Red horizontal line represents the boundary between cells with 2 N and > 2 N DNA content. ( c ) DNA/EdU distributions from 25 000 cells with either low, intermediate or high YFP marked with red boxes in a . The percentage of EdU-positive cells is indicated. ( d ) Data in a were analyzed by bin analysis where cells were divided into 21 bins according to their YFP fluorescence. The first bin contained all cells with YFP fluorescence under 100 a.u. (range of uninduced samples). Red dots represent proportion of EdU-positive cells for each YFP bin. Blue dot represents uninduced control. ( e ) Bin analysis as in d for DNA content. ( f ) Analysis as in d for three cancer cell lines and HME normal cells using 60 YFP bins. ( g ) Analysis as in e for three cancer cell lines and HME normal cells using 60 YFP bins
    Ptripz Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptripz vector/product/Thermo Fisher
    Average 93 stars, based on 333 article reviews
    Price from $9.99 to $1999.99
    ptripz vector - by Bioz Stars, 2020-05
    93/100 stars
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    89
    GE Healthcare ptripz vector
    Dose-dependent effect of E2F1 on DNA synthesis and content. Cells expressing <t>pTRIPZ-YFP-ER-E2F1</t> were starved of growth factors for 2 days and then treated with a combination of 1 μ g/ml doxycycline, 90 nM OHT or control starvation medium in the presence of 10 μ M EdU. Cells were fixed and stained for DNA content (DAPI) and DNA synthesis (EdU incorporation) 20 h later. ( a ) YFP/EdU distribution from induced HME cells. Red horizontal dashed line represents the boundary between EdU-positive and EdU-negative cells. Regions of low, intermediate and high YFP used for plotting DNA/EdU distributions in ( c ) are marked with red boxes. ( b ) YFP/DNA distribution from induced HME cells. Red horizontal line represents the boundary between cells with 2 N and > 2 N DNA content. ( c ) DNA/EdU distributions from 25 000 cells with either low, intermediate or high YFP marked with red boxes in a . The percentage of EdU-positive cells is indicated. ( d ) Data in a were analyzed by bin analysis where cells were divided into 21 bins according to their YFP fluorescence. The first bin contained all cells with YFP fluorescence under 100 a.u. (range of uninduced samples). Red dots represent proportion of EdU-positive cells for each YFP bin. Blue dot represents uninduced control. ( e ) Bin analysis as in d for DNA content. ( f ) Analysis as in d for three cancer cell lines and HME normal cells using 60 YFP bins. ( g ) Analysis as in e for three cancer cell lines and HME normal cells using 60 YFP bins
    Ptripz Vector, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptripz vector/product/GE Healthcare
    Average 89 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    ptripz vector - by Bioz Stars, 2020-05
    89/100 stars
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    89
    Horizon Discovery ptripz vector
    Dose-dependent effect of E2F1 on DNA synthesis and content. Cells expressing <t>pTRIPZ-YFP-ER-E2F1</t> were starved of growth factors for 2 days and then treated with a combination of 1 μ g/ml doxycycline, 90 nM OHT or control starvation medium in the presence of 10 μ M EdU. Cells were fixed and stained for DNA content (DAPI) and DNA synthesis (EdU incorporation) 20 h later. ( a ) YFP/EdU distribution from induced HME cells. Red horizontal dashed line represents the boundary between EdU-positive and EdU-negative cells. Regions of low, intermediate and high YFP used for plotting DNA/EdU distributions in ( c ) are marked with red boxes. ( b ) YFP/DNA distribution from induced HME cells. Red horizontal line represents the boundary between cells with 2 N and > 2 N DNA content. ( c ) DNA/EdU distributions from 25 000 cells with either low, intermediate or high YFP marked with red boxes in a . The percentage of EdU-positive cells is indicated. ( d ) Data in a were analyzed by bin analysis where cells were divided into 21 bins according to their YFP fluorescence. The first bin contained all cells with YFP fluorescence under 100 a.u. (range of uninduced samples). Red dots represent proportion of EdU-positive cells for each YFP bin. Blue dot represents uninduced control. ( e ) Bin analysis as in d for DNA content. ( f ) Analysis as in d for three cancer cell lines and HME normal cells using 60 YFP bins. ( g ) Analysis as in e for three cancer cell lines and HME normal cells using 60 YFP bins
    Ptripz Vector, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 89/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptripz vector/product/Horizon Discovery
    Average 89 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    ptripz vector - by Bioz Stars, 2020-05
    89/100 stars
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    89
    TaKaRa ptripz vector
    Dose-dependent effect of E2F1 on DNA synthesis and content. Cells expressing <t>pTRIPZ-YFP-ER-E2F1</t> were starved of growth factors for 2 days and then treated with a combination of 1 μ g/ml doxycycline, 90 nM OHT or control starvation medium in the presence of 10 μ M EdU. Cells were fixed and stained for DNA content (DAPI) and DNA synthesis (EdU incorporation) 20 h later. ( a ) YFP/EdU distribution from induced HME cells. Red horizontal dashed line represents the boundary between EdU-positive and EdU-negative cells. Regions of low, intermediate and high YFP used for plotting DNA/EdU distributions in ( c ) are marked with red boxes. ( b ) YFP/DNA distribution from induced HME cells. Red horizontal line represents the boundary between cells with 2 N and > 2 N DNA content. ( c ) DNA/EdU distributions from 25 000 cells with either low, intermediate or high YFP marked with red boxes in a . The percentage of EdU-positive cells is indicated. ( d ) Data in a were analyzed by bin analysis where cells were divided into 21 bins according to their YFP fluorescence. The first bin contained all cells with YFP fluorescence under 100 a.u. (range of uninduced samples). Red dots represent proportion of EdU-positive cells for each YFP bin. Blue dot represents uninduced control. ( e ) Bin analysis as in d for DNA content. ( f ) Analysis as in d for three cancer cell lines and HME normal cells using 60 YFP bins. ( g ) Analysis as in e for three cancer cell lines and HME normal cells using 60 YFP bins
    Ptripz Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptripz vector/product/TaKaRa
    Average 89 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    ptripz vector - by Bioz Stars, 2020-05
    89/100 stars
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    85
    Thermo Fisher ptripz inducible vector
    <t>p53</t> wild-type increases the sensitivity of CD22-positive cells to Inotuzumab Ozogamicin. (A) BL-2 and SUP-B15 cell lines were lentivirally transduced with an empty vector (EV) or the GFP-tagged p53 R248Q -pLEX construct. After puromycin selection, cells were lysed and protein extracts were blotted using an anti-GFP antibody to verify protein expression. Actin was employed as a loading control. (B) The modified cell lines, stably expressing either EV (•) or mutant p53 (■) were then exposed to logarithmic doses of IO and their reduction in cell proliferation was evaluated employing the ATPLite luminescence assay. Results represent the average ± standard deviation of at least three different experiments performed in triplicates with relative luminescence of untreated cells arbitrarily set at 100%. IC 50 values were calculated by logistic non-linear regression and are presented as nM equivalents of CalichDMH. (C) Namalwa cells were transduced with an empty vector (EV) or GFP-tagged wild-type (WT) <t>p53—pTRIPZ</t> vector using an inducible lentiviral system. After puromycin selection, cells were treated for 24 h with doxycycline to induce wild-type p53 expression. Cells were harvested, lysed and protein extracts were simultaneously blotted for p53 and Actin, the latter employed as a loading control. (D) The reductions in proliferation of Namalwa cells expressing EV (•) or p53 WT (■) were then evaluated after co-treatment for 24 h with doxycycline and logarithmic doses of CalichDMH. Results represent the average ± standard deviation of at least three different experiments performed in triplicates with relative luminescence of untreated cells set arbitrarily at 100%. IC 50 values are reported as nM equivalents of CalichDMH.
    Ptripz Inducible Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptripz inducible vector/product/Thermo Fisher
    Average 85 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    ptripz inducible vector - by Bioz Stars, 2020-05
    85/100 stars
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    88
    Thermo Fisher tet on ptripz vector
    <t>p53</t> wild-type increases the sensitivity of CD22-positive cells to Inotuzumab Ozogamicin. (A) BL-2 and SUP-B15 cell lines were lentivirally transduced with an empty vector (EV) or the GFP-tagged p53 R248Q -pLEX construct. After puromycin selection, cells were lysed and protein extracts were blotted using an anti-GFP antibody to verify protein expression. Actin was employed as a loading control. (B) The modified cell lines, stably expressing either EV (•) or mutant p53 (■) were then exposed to logarithmic doses of IO and their reduction in cell proliferation was evaluated employing the ATPLite luminescence assay. Results represent the average ± standard deviation of at least three different experiments performed in triplicates with relative luminescence of untreated cells arbitrarily set at 100%. IC 50 values were calculated by logistic non-linear regression and are presented as nM equivalents of CalichDMH. (C) Namalwa cells were transduced with an empty vector (EV) or GFP-tagged wild-type (WT) <t>p53—pTRIPZ</t> vector using an inducible lentiviral system. After puromycin selection, cells were treated for 24 h with doxycycline to induce wild-type p53 expression. Cells were harvested, lysed and protein extracts were simultaneously blotted for p53 and Actin, the latter employed as a loading control. (D) The reductions in proliferation of Namalwa cells expressing EV (•) or p53 WT (■) were then evaluated after co-treatment for 24 h with doxycycline and logarithmic doses of CalichDMH. Results represent the average ± standard deviation of at least three different experiments performed in triplicates with relative luminescence of untreated cells set arbitrarily at 100%. IC 50 values are reported as nM equivalents of CalichDMH.
    Tet On Ptripz Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tet on ptripz vector/product/Thermo Fisher
    Average 88 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    tet on ptripz vector - by Bioz Stars, 2020-05
    88/100 stars
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    88
    Thermo Fisher ptripz control vector
    <t>p53</t> wild-type increases the sensitivity of CD22-positive cells to Inotuzumab Ozogamicin. (A) BL-2 and SUP-B15 cell lines were lentivirally transduced with an empty vector (EV) or the GFP-tagged p53 R248Q -pLEX construct. After puromycin selection, cells were lysed and protein extracts were blotted using an anti-GFP antibody to verify protein expression. Actin was employed as a loading control. (B) The modified cell lines, stably expressing either EV (•) or mutant p53 (■) were then exposed to logarithmic doses of IO and their reduction in cell proliferation was evaluated employing the ATPLite luminescence assay. Results represent the average ± standard deviation of at least three different experiments performed in triplicates with relative luminescence of untreated cells arbitrarily set at 100%. IC 50 values were calculated by logistic non-linear regression and are presented as nM equivalents of CalichDMH. (C) Namalwa cells were transduced with an empty vector (EV) or GFP-tagged wild-type (WT) <t>p53—pTRIPZ</t> vector using an inducible lentiviral system. After puromycin selection, cells were treated for 24 h with doxycycline to induce wild-type p53 expression. Cells were harvested, lysed and protein extracts were simultaneously blotted for p53 and Actin, the latter employed as a loading control. (D) The reductions in proliferation of Namalwa cells expressing EV (•) or p53 WT (■) were then evaluated after co-treatment for 24 h with doxycycline and logarithmic doses of CalichDMH. Results represent the average ± standard deviation of at least three different experiments performed in triplicates with relative luminescence of untreated cells set arbitrarily at 100%. IC 50 values are reported as nM equivalents of CalichDMH.
    Ptripz Control Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptripz control vector/product/Thermo Fisher
    Average 88 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    ptripz control vector - by Bioz Stars, 2020-05
    88/100 stars
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    93
    Thermo Fisher ptripz lenti vector
    <t>p53</t> wild-type increases the sensitivity of CD22-positive cells to Inotuzumab Ozogamicin. (A) BL-2 and SUP-B15 cell lines were lentivirally transduced with an empty vector (EV) or the GFP-tagged p53 R248Q -pLEX construct. After puromycin selection, cells were lysed and protein extracts were blotted using an anti-GFP antibody to verify protein expression. Actin was employed as a loading control. (B) The modified cell lines, stably expressing either EV (•) or mutant p53 (■) were then exposed to logarithmic doses of IO and their reduction in cell proliferation was evaluated employing the ATPLite luminescence assay. Results represent the average ± standard deviation of at least three different experiments performed in triplicates with relative luminescence of untreated cells arbitrarily set at 100%. IC 50 values were calculated by logistic non-linear regression and are presented as nM equivalents of CalichDMH. (C) Namalwa cells were transduced with an empty vector (EV) or GFP-tagged wild-type (WT) <t>p53—pTRIPZ</t> vector using an inducible lentiviral system. After puromycin selection, cells were treated for 24 h with doxycycline to induce wild-type p53 expression. Cells were harvested, lysed and protein extracts were simultaneously blotted for p53 and Actin, the latter employed as a loading control. (D) The reductions in proliferation of Namalwa cells expressing EV (•) or p53 WT (■) were then evaluated after co-treatment for 24 h with doxycycline and logarithmic doses of CalichDMH. Results represent the average ± standard deviation of at least three different experiments performed in triplicates with relative luminescence of untreated cells set arbitrarily at 100%. IC 50 values are reported as nM equivalents of CalichDMH.
    Ptripz Lenti Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptripz lenti vector/product/Thermo Fisher
    Average 93 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    ptripz lenti vector - by Bioz Stars, 2020-05
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    88
    Thermo Fisher ptripz lentiviral vector
    Expression levels of miR-148a across four molecular subgroups of medulloblastoma, cell lines and miR-148a expressing stable polyclonal populations, as determined by real time RT-PCR assay (A) miR-148a levels were evaluated in 141 medulloblastoma tissues classified in the four molecular subgroups, normal developing (from less than 1year old infants) cerebellar tissues and adult (from individuals > 21 yr of age) cerebellar tissues. (B, C, D) Relative Quantity of miR-148a in the indicated medulloblastoma cell line and its polyclonal populations transduced with <t>pTRIPZ</t> control <t>lentiviral</t> vector (Vector) or pTRIPZ-miR-148a construct expressing miR-148a in doxycycline inducible manner (P1, P2) and in the cells transiently transfected with control siGLO or miR-148a mimic. + DOX: doxycycline induced.
    Ptripz Lentiviral Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptripz lentiviral vector/product/Thermo Fisher
    Average 88 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    ptripz lentiviral vector - by Bioz Stars, 2020-05
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    90
    Thermo Fisher vector ptripz empty
    Expression levels of miR-148a across four molecular subgroups of medulloblastoma, cell lines and miR-148a expressing stable polyclonal populations, as determined by real time RT-PCR assay (A) miR-148a levels were evaluated in 141 medulloblastoma tissues classified in the four molecular subgroups, normal developing (from less than 1year old infants) cerebellar tissues and adult (from individuals > 21 yr of age) cerebellar tissues. (B, C, D) Relative Quantity of miR-148a in the indicated medulloblastoma cell line and its polyclonal populations transduced with <t>pTRIPZ</t> control <t>lentiviral</t> vector (Vector) or pTRIPZ-miR-148a construct expressing miR-148a in doxycycline inducible manner (P1, P2) and in the cells transiently transfected with control siGLO or miR-148a mimic. + DOX: doxycycline induced.
    Vector Ptripz Empty, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector ptripz empty/product/Thermo Fisher
    Average 90 stars, based on 9 article reviews
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    vector ptripz empty - by Bioz Stars, 2020-05
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    93
    Horizon Discovery ptripz lentiviral vector
    Expression levels of miR-148a across four molecular subgroups of medulloblastoma, cell lines and miR-148a expressing stable polyclonal populations, as determined by real time RT-PCR assay (A) miR-148a levels were evaluated in 141 medulloblastoma tissues classified in the four molecular subgroups, normal developing (from less than 1year old infants) cerebellar tissues and adult (from individuals > 21 yr of age) cerebellar tissues. (B, C, D) Relative Quantity of miR-148a in the indicated medulloblastoma cell line and its polyclonal populations transduced with <t>pTRIPZ</t> control <t>lentiviral</t> vector (Vector) or pTRIPZ-miR-148a construct expressing miR-148a in doxycycline inducible manner (P1, P2) and in the cells transiently transfected with control siGLO or miR-148a mimic. + DOX: doxycycline induced.
    Ptripz Lentiviral Vector, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptripz lentiviral vector/product/Horizon Discovery
    Average 93 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    ptripz lentiviral vector - by Bioz Stars, 2020-05
    93/100 stars
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    88
    Thermo Fisher expression vector ptripz
    Expression levels of miR-148a across four molecular subgroups of medulloblastoma, cell lines and miR-148a expressing stable polyclonal populations, as determined by real time RT-PCR assay (A) miR-148a levels were evaluated in 141 medulloblastoma tissues classified in the four molecular subgroups, normal developing (from less than 1year old infants) cerebellar tissues and adult (from individuals > 21 yr of age) cerebellar tissues. (B, C, D) Relative Quantity of miR-148a in the indicated medulloblastoma cell line and its polyclonal populations transduced with <t>pTRIPZ</t> control <t>lentiviral</t> vector (Vector) or pTRIPZ-miR-148a construct expressing miR-148a in doxycycline inducible manner (P1, P2) and in the cells transiently transfected with control siGLO or miR-148a mimic. + DOX: doxycycline induced.
    Expression Vector Ptripz, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression vector ptripz/product/Thermo Fisher
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    expression vector ptripz - by Bioz Stars, 2020-05
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    91
    Thermo Fisher tet on ptripz lentiviral vector
    Expression levels of miR-148a across four molecular subgroups of medulloblastoma, cell lines and miR-148a expressing stable polyclonal populations, as determined by real time RT-PCR assay (A) miR-148a levels were evaluated in 141 medulloblastoma tissues classified in the four molecular subgroups, normal developing (from less than 1year old infants) cerebellar tissues and adult (from individuals > 21 yr of age) cerebellar tissues. (B, C, D) Relative Quantity of miR-148a in the indicated medulloblastoma cell line and its polyclonal populations transduced with <t>pTRIPZ</t> control <t>lentiviral</t> vector (Vector) or pTRIPZ-miR-148a construct expressing miR-148a in doxycycline inducible manner (P1, P2) and in the cells transiently transfected with control siGLO or miR-148a mimic. + DOX: doxycycline induced.
    Tet On Ptripz Lentiviral Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tet on ptripz lentiviral vector/product/Thermo Fisher
    Average 91 stars, based on 9 article reviews
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    tet on ptripz lentiviral vector - by Bioz Stars, 2020-05
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    86
    GE Healthcare blunt ended ptripz vector
    Expression levels of miR-148a across four molecular subgroups of medulloblastoma, cell lines and miR-148a expressing stable polyclonal populations, as determined by real time RT-PCR assay (A) miR-148a levels were evaluated in 141 medulloblastoma tissues classified in the four molecular subgroups, normal developing (from less than 1year old infants) cerebellar tissues and adult (from individuals > 21 yr of age) cerebellar tissues. (B, C, D) Relative Quantity of miR-148a in the indicated medulloblastoma cell line and its polyclonal populations transduced with <t>pTRIPZ</t> control <t>lentiviral</t> vector (Vector) or pTRIPZ-miR-148a construct expressing miR-148a in doxycycline inducible manner (P1, P2) and in the cells transiently transfected with control siGLO or miR-148a mimic. + DOX: doxycycline induced.
    Blunt Ended Ptripz Vector, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blunt ended ptripz vector/product/GE Healthcare
    Average 86 stars, based on 5 article reviews
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    blunt ended ptripz vector - by Bioz Stars, 2020-05
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    85
    Thermo Fisher lentiviral ptripz shrnamir vector
    Expression levels of miR-148a across four molecular subgroups of medulloblastoma, cell lines and miR-148a expressing stable polyclonal populations, as determined by real time RT-PCR assay (A) miR-148a levels were evaluated in 141 medulloblastoma tissues classified in the four molecular subgroups, normal developing (from less than 1year old infants) cerebellar tissues and adult (from individuals > 21 yr of age) cerebellar tissues. (B, C, D) Relative Quantity of miR-148a in the indicated medulloblastoma cell line and its polyclonal populations transduced with <t>pTRIPZ</t> control <t>lentiviral</t> vector (Vector) or pTRIPZ-miR-148a construct expressing miR-148a in doxycycline inducible manner (P1, P2) and in the cells transiently transfected with control siGLO or miR-148a mimic. + DOX: doxycycline induced.
    Lentiviral Ptripz Shrnamir Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 18 article reviews
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    lentiviral ptripz shrnamir vector - by Bioz Stars, 2020-05
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    88
    Thermo Fisher ptripz empty ptctrl vector
    Expression levels of miR-148a across four molecular subgroups of medulloblastoma, cell lines and miR-148a expressing stable polyclonal populations, as determined by real time RT-PCR assay (A) miR-148a levels were evaluated in 141 medulloblastoma tissues classified in the four molecular subgroups, normal developing (from less than 1year old infants) cerebellar tissues and adult (from individuals > 21 yr of age) cerebellar tissues. (B, C, D) Relative Quantity of miR-148a in the indicated medulloblastoma cell line and its polyclonal populations transduced with <t>pTRIPZ</t> control <t>lentiviral</t> vector (Vector) or pTRIPZ-miR-148a construct expressing miR-148a in doxycycline inducible manner (P1, P2) and in the cells transiently transfected with control siGLO or miR-148a mimic. + DOX: doxycycline induced.
    Ptripz Empty Ptctrl Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 7 article reviews
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    ptripz empty ptctrl vector - by Bioz Stars, 2020-05
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    89
    Addgene inc ptripz vector
    Expression levels of miR-148a across four molecular subgroups of medulloblastoma, cell lines and miR-148a expressing stable polyclonal populations, as determined by real time RT-PCR assay (A) miR-148a levels were evaluated in 141 medulloblastoma tissues classified in the four molecular subgroups, normal developing (from less than 1year old infants) cerebellar tissues and adult (from individuals > 21 yr of age) cerebellar tissues. (B, C, D) Relative Quantity of miR-148a in the indicated medulloblastoma cell line and its polyclonal populations transduced with <t>pTRIPZ</t> control <t>lentiviral</t> vector (Vector) or pTRIPZ-miR-148a construct expressing miR-148a in doxycycline inducible manner (P1, P2) and in the cells transiently transfected with control siGLO or miR-148a mimic. + DOX: doxycycline induced.
    Ptripz Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptripz vector/product/Addgene inc
    Average 89 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    ptripz vector - by Bioz Stars, 2020-05
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    86
    Thermo Fisher ptripz inducible lentiviral vector
    Expression levels of miR-148a across four molecular subgroups of medulloblastoma, cell lines and miR-148a expressing stable polyclonal populations, as determined by real time RT-PCR assay (A) miR-148a levels were evaluated in 141 medulloblastoma tissues classified in the four molecular subgroups, normal developing (from less than 1year old infants) cerebellar tissues and adult (from individuals > 21 yr of age) cerebellar tissues. (B, C, D) Relative Quantity of miR-148a in the indicated medulloblastoma cell line and its polyclonal populations transduced with <t>pTRIPZ</t> control <t>lentiviral</t> vector (Vector) or pTRIPZ-miR-148a construct expressing miR-148a in doxycycline inducible manner (P1, P2) and in the cells transiently transfected with control siGLO or miR-148a mimic. + DOX: doxycycline induced.
    Ptripz Inducible Lentiviral Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptripz inducible lentiviral vector/product/Thermo Fisher
    Average 86 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    ptripz inducible lentiviral vector - by Bioz Stars, 2020-05
    86/100 stars
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    Dose-dependent effect of E2F1 on DNA synthesis and content. Cells expressing pTRIPZ-YFP-ER-E2F1 were starved of growth factors for 2 days and then treated with a combination of 1 μ g/ml doxycycline, 90 nM OHT or control starvation medium in the presence of 10 μ M EdU. Cells were fixed and stained for DNA content (DAPI) and DNA synthesis (EdU incorporation) 20 h later. ( a ) YFP/EdU distribution from induced HME cells. Red horizontal dashed line represents the boundary between EdU-positive and EdU-negative cells. Regions of low, intermediate and high YFP used for plotting DNA/EdU distributions in ( c ) are marked with red boxes. ( b ) YFP/DNA distribution from induced HME cells. Red horizontal line represents the boundary between cells with 2 N and > 2 N DNA content. ( c ) DNA/EdU distributions from 25 000 cells with either low, intermediate or high YFP marked with red boxes in a . The percentage of EdU-positive cells is indicated. ( d ) Data in a were analyzed by bin analysis where cells were divided into 21 bins according to their YFP fluorescence. The first bin contained all cells with YFP fluorescence under 100 a.u. (range of uninduced samples). Red dots represent proportion of EdU-positive cells for each YFP bin. Blue dot represents uninduced control. ( e ) Bin analysis as in d for DNA content. ( f ) Analysis as in d for three cancer cell lines and HME normal cells using 60 YFP bins. ( g ) Analysis as in e for three cancer cell lines and HME normal cells using 60 YFP bins

    Journal: Cell Death and Differentiation

    Article Title: Expression level is a key determinant of E2F1-mediated cell fate

    doi: 10.1038/cdd.2017.12

    Figure Lengend Snippet: Dose-dependent effect of E2F1 on DNA synthesis and content. Cells expressing pTRIPZ-YFP-ER-E2F1 were starved of growth factors for 2 days and then treated with a combination of 1 μ g/ml doxycycline, 90 nM OHT or control starvation medium in the presence of 10 μ M EdU. Cells were fixed and stained for DNA content (DAPI) and DNA synthesis (EdU incorporation) 20 h later. ( a ) YFP/EdU distribution from induced HME cells. Red horizontal dashed line represents the boundary between EdU-positive and EdU-negative cells. Regions of low, intermediate and high YFP used for plotting DNA/EdU distributions in ( c ) are marked with red boxes. ( b ) YFP/DNA distribution from induced HME cells. Red horizontal line represents the boundary between cells with 2 N and > 2 N DNA content. ( c ) DNA/EdU distributions from 25 000 cells with either low, intermediate or high YFP marked with red boxes in a . The percentage of EdU-positive cells is indicated. ( d ) Data in a were analyzed by bin analysis where cells were divided into 21 bins according to their YFP fluorescence. The first bin contained all cells with YFP fluorescence under 100 a.u. (range of uninduced samples). Red dots represent proportion of EdU-positive cells for each YFP bin. Blue dot represents uninduced control. ( e ) Bin analysis as in d for DNA content. ( f ) Analysis as in d for three cancer cell lines and HME normal cells using 60 YFP bins. ( g ) Analysis as in e for three cancer cell lines and HME normal cells using 60 YFP bins

    Article Snippet: pEYFP-ER-E2F1 construct was generated by inserting the Nhe I digestion fragment containing the ER-E2F1 cassette from pBabePuro-HA.ER.E2F1 (a gift from Dr. Kristian Helin) into the compatible Xba I digestion site of the pEYFP-C1 vector (Clontech, Mountain View, CA, USA). pTRIPZ-YFP-ER-E2F1 construct was generated by inserting the YFP-ER-E2F1 cassette from pEYFP-ER-E2F1 into the pTRIPZ vector (Open Biosystems, Lafayette, CO, USA) between the Age I and Mlu I sites replacing the TurboRFP and shRNA cassette.

    Techniques: DNA Synthesis, Expressing, Staining, Fluorescence

    E2F1 induces mitotic arrest in multiple cell lines. ( a ) HME cells expressing pTRIPZ-YFP-ER-E2F1 in full growth factor containing medium were treated with a combination of 1 μ g/ml doxycycline and 90 nM OHT for 24 h. Cells were stained with anti-phospho-histone H3 antibody (mitotic marker) and analyzed by flow cytometry. ( b ) Six cell lines expressing pTRIPZ-YFP-ER-E2F1 in full serum-containing medium were treated with a combination of 1 μ for the raw data

    Journal: Cell Death and Differentiation

    Article Title: Expression level is a key determinant of E2F1-mediated cell fate

    doi: 10.1038/cdd.2017.12

    Figure Lengend Snippet: E2F1 induces mitotic arrest in multiple cell lines. ( a ) HME cells expressing pTRIPZ-YFP-ER-E2F1 in full growth factor containing medium were treated with a combination of 1 μ g/ml doxycycline and 90 nM OHT for 24 h. Cells were stained with anti-phospho-histone H3 antibody (mitotic marker) and analyzed by flow cytometry. ( b ) Six cell lines expressing pTRIPZ-YFP-ER-E2F1 in full serum-containing medium were treated with a combination of 1 μ for the raw data

    Article Snippet: pEYFP-ER-E2F1 construct was generated by inserting the Nhe I digestion fragment containing the ER-E2F1 cassette from pBabePuro-HA.ER.E2F1 (a gift from Dr. Kristian Helin) into the compatible Xba I digestion site of the pEYFP-C1 vector (Clontech, Mountain View, CA, USA). pTRIPZ-YFP-ER-E2F1 construct was generated by inserting the YFP-ER-E2F1 cassette from pEYFP-ER-E2F1 into the pTRIPZ vector (Open Biosystems, Lafayette, CO, USA) between the Age I and Mlu I sites replacing the TurboRFP and shRNA cassette.

    Techniques: Expressing, Staining, Marker, Flow Cytometry, Cytometry

    Experimental system for studies of E2F1-mediated cell fates at a single-cell resolution. ( a ) Schematic of YFP–ER–E2F1 fusion protein. ( b ) U2OS cells stably expressing pEYFP-ER-E2F1 were serum starved for 24 h and then treated with 2 μ M tamoxifen in starvation medium. Cells were collected at indicated time points and expression of BIM, APAF1, FOXO3 and CASP3 analyzed by real-time qPCR. ( c ) U2OS cells stably expressing pEYFP-ER-E2F1 were serum starved for 24 h and then treated with 2 μ M tamoxifen in starvation medium. Cells were collected at indicated time points and PARP cleavage was analyzed by western blot. ( d ) Images of U2OS cells expressing YFP-ER-E2F1 (green) and H2B-RFP (red) before (0 h), 4 h and 40 h after exposure to 3 μ M tamoxifen. ( e ) Schematic of pTRIPZ-YFP-ER-E2F1 construct. ( f and g ) U2OS pTRIPZ-YFP-ER-E2F1 cells were grown in full serum-containing growth medium and treated with 500 ng/ml doxycycline for 48 h followed by addition of 90 nM OHT for an additional 20 h. Cells from the indicated YFP fractions were sorted by flow cytometry and E2F1 expression was analyzed by quantitative RT-PCR. ( f ) Histogram of YFP expression and sorting gates in induced U2OS pTRIPZ-YFP-ER-E2F1 cells (red) and parental U2OS cells (blue). ( g ) Real-time quantitative RT-PCR analysis of total E2F1 (endogenous+YFP-ER-E2F1) in sorted fractions. Graph represents the relative E2F1 expression after normalization to GAPDH housekeeping control (mean and range of duplicate PCR reactions). IRES, internal ribosomal entry site; rtTA3, reverse tetracycline-transactivator 3; TRE, tetracycline-responsive element; UBC, ubiquitin C promoter

    Journal: Cell Death and Differentiation

    Article Title: Expression level is a key determinant of E2F1-mediated cell fate

    doi: 10.1038/cdd.2017.12

    Figure Lengend Snippet: Experimental system for studies of E2F1-mediated cell fates at a single-cell resolution. ( a ) Schematic of YFP–ER–E2F1 fusion protein. ( b ) U2OS cells stably expressing pEYFP-ER-E2F1 were serum starved for 24 h and then treated with 2 μ M tamoxifen in starvation medium. Cells were collected at indicated time points and expression of BIM, APAF1, FOXO3 and CASP3 analyzed by real-time qPCR. ( c ) U2OS cells stably expressing pEYFP-ER-E2F1 were serum starved for 24 h and then treated with 2 μ M tamoxifen in starvation medium. Cells were collected at indicated time points and PARP cleavage was analyzed by western blot. ( d ) Images of U2OS cells expressing YFP-ER-E2F1 (green) and H2B-RFP (red) before (0 h), 4 h and 40 h after exposure to 3 μ M tamoxifen. ( e ) Schematic of pTRIPZ-YFP-ER-E2F1 construct. ( f and g ) U2OS pTRIPZ-YFP-ER-E2F1 cells were grown in full serum-containing growth medium and treated with 500 ng/ml doxycycline for 48 h followed by addition of 90 nM OHT for an additional 20 h. Cells from the indicated YFP fractions were sorted by flow cytometry and E2F1 expression was analyzed by quantitative RT-PCR. ( f ) Histogram of YFP expression and sorting gates in induced U2OS pTRIPZ-YFP-ER-E2F1 cells (red) and parental U2OS cells (blue). ( g ) Real-time quantitative RT-PCR analysis of total E2F1 (endogenous+YFP-ER-E2F1) in sorted fractions. Graph represents the relative E2F1 expression after normalization to GAPDH housekeeping control (mean and range of duplicate PCR reactions). IRES, internal ribosomal entry site; rtTA3, reverse tetracycline-transactivator 3; TRE, tetracycline-responsive element; UBC, ubiquitin C promoter

    Article Snippet: pEYFP-ER-E2F1 construct was generated by inserting the Nhe I digestion fragment containing the ER-E2F1 cassette from pBabePuro-HA.ER.E2F1 (a gift from Dr. Kristian Helin) into the compatible Xba I digestion site of the pEYFP-C1 vector (Clontech, Mountain View, CA, USA). pTRIPZ-YFP-ER-E2F1 construct was generated by inserting the YFP-ER-E2F1 cassette from pEYFP-ER-E2F1 into the pTRIPZ vector (Open Biosystems, Lafayette, CO, USA) between the Age I and Mlu I sites replacing the TurboRFP and shRNA cassette.

    Techniques: Stable Transfection, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Construct, Flow Cytometry, Cytometry, Quantitative RT-PCR, Polymerase Chain Reaction

    p53 wild-type increases the sensitivity of CD22-positive cells to Inotuzumab Ozogamicin. (A) BL-2 and SUP-B15 cell lines were lentivirally transduced with an empty vector (EV) or the GFP-tagged p53 R248Q -pLEX construct. After puromycin selection, cells were lysed and protein extracts were blotted using an anti-GFP antibody to verify protein expression. Actin was employed as a loading control. (B) The modified cell lines, stably expressing either EV (•) or mutant p53 (■) were then exposed to logarithmic doses of IO and their reduction in cell proliferation was evaluated employing the ATPLite luminescence assay. Results represent the average ± standard deviation of at least three different experiments performed in triplicates with relative luminescence of untreated cells arbitrarily set at 100%. IC 50 values were calculated by logistic non-linear regression and are presented as nM equivalents of CalichDMH. (C) Namalwa cells were transduced with an empty vector (EV) or GFP-tagged wild-type (WT) p53—pTRIPZ vector using an inducible lentiviral system. After puromycin selection, cells were treated for 24 h with doxycycline to induce wild-type p53 expression. Cells were harvested, lysed and protein extracts were simultaneously blotted for p53 and Actin, the latter employed as a loading control. (D) The reductions in proliferation of Namalwa cells expressing EV (•) or p53 WT (■) were then evaluated after co-treatment for 24 h with doxycycline and logarithmic doses of CalichDMH. Results represent the average ± standard deviation of at least three different experiments performed in triplicates with relative luminescence of untreated cells set arbitrarily at 100%. IC 50 values are reported as nM equivalents of CalichDMH.

    Journal: Frontiers in Oncology

    Article Title: Chk1 Inhibition Restores Inotuzumab Ozogamicin Citotoxicity in CD22-Positive Cells Expressing Mutant p53

    doi: 10.3389/fonc.2019.00057

    Figure Lengend Snippet: p53 wild-type increases the sensitivity of CD22-positive cells to Inotuzumab Ozogamicin. (A) BL-2 and SUP-B15 cell lines were lentivirally transduced with an empty vector (EV) or the GFP-tagged p53 R248Q -pLEX construct. After puromycin selection, cells were lysed and protein extracts were blotted using an anti-GFP antibody to verify protein expression. Actin was employed as a loading control. (B) The modified cell lines, stably expressing either EV (•) or mutant p53 (■) were then exposed to logarithmic doses of IO and their reduction in cell proliferation was evaluated employing the ATPLite luminescence assay. Results represent the average ± standard deviation of at least three different experiments performed in triplicates with relative luminescence of untreated cells arbitrarily set at 100%. IC 50 values were calculated by logistic non-linear regression and are presented as nM equivalents of CalichDMH. (C) Namalwa cells were transduced with an empty vector (EV) or GFP-tagged wild-type (WT) p53—pTRIPZ vector using an inducible lentiviral system. After puromycin selection, cells were treated for 24 h with doxycycline to induce wild-type p53 expression. Cells were harvested, lysed and protein extracts were simultaneously blotted for p53 and Actin, the latter employed as a loading control. (D) The reductions in proliferation of Namalwa cells expressing EV (•) or p53 WT (■) were then evaluated after co-treatment for 24 h with doxycycline and logarithmic doses of CalichDMH. Results represent the average ± standard deviation of at least three different experiments performed in triplicates with relative luminescence of untreated cells set arbitrarily at 100%. IC 50 values are reported as nM equivalents of CalichDMH.

    Article Snippet: To create a human inducible p53 wild-type-EGFP construct, p53 and EGFP were separately cloned in the inducible pTRIPz vector (Open Biosystem).

    Techniques: Transduction, Plasmid Preparation, Construct, Selection, Expressing, Modification, Stable Transfection, Mutagenesis, Luminescence Assay, Standard Deviation

    Expression levels of miR-148a across four molecular subgroups of medulloblastoma, cell lines and miR-148a expressing stable polyclonal populations, as determined by real time RT-PCR assay (A) miR-148a levels were evaluated in 141 medulloblastoma tissues classified in the four molecular subgroups, normal developing (from less than 1year old infants) cerebellar tissues and adult (from individuals > 21 yr of age) cerebellar tissues. (B, C, D) Relative Quantity of miR-148a in the indicated medulloblastoma cell line and its polyclonal populations transduced with pTRIPZ control lentiviral vector (Vector) or pTRIPZ-miR-148a construct expressing miR-148a in doxycycline inducible manner (P1, P2) and in the cells transiently transfected with control siGLO or miR-148a mimic. + DOX: doxycycline induced.

    Journal: Oncoscience

    Article Title: MiR-148a, a microRNA upregulated in the WNT subgroup tumors, inhibits invasion and tumorigenic potential of medulloblastoma cells by targeting Neuropilin 1

    doi:

    Figure Lengend Snippet: Expression levels of miR-148a across four molecular subgroups of medulloblastoma, cell lines and miR-148a expressing stable polyclonal populations, as determined by real time RT-PCR assay (A) miR-148a levels were evaluated in 141 medulloblastoma tissues classified in the four molecular subgroups, normal developing (from less than 1year old infants) cerebellar tissues and adult (from individuals > 21 yr of age) cerebellar tissues. (B, C, D) Relative Quantity of miR-148a in the indicated medulloblastoma cell line and its polyclonal populations transduced with pTRIPZ control lentiviral vector (Vector) or pTRIPZ-miR-148a construct expressing miR-148a in doxycycline inducible manner (P1, P2) and in the cells transiently transfected with control siGLO or miR-148a mimic. + DOX: doxycycline induced.

    Article Snippet: Transduction of medulloblastoma cells with miRNA expressing lentiviral vectors Genomic region encoding miR-148a was amplified from normal human lymphocyte DNA by Polymerase Chain reaction (PCR) and cloned in a pTRIPZ lentiviral vector (Open Biosystems, Thermo Scientific, Lafayette, CO, USA) downstream of doxycycline-inducible minimal Cytomegalo virus (CMV) promoter.

    Techniques: Expressing, Quantitative RT-PCR, Transduction, Plasmid Preparation, Construct, Transfection

    Knockdown of NF1 in Human Endothelial Cells is Sufficient to Activate Ras and Induce Cellular Signaling. (A) Early passage HUVECs were infected with a pSIREN-GFP retroviral vector carrying either a control or a NF1 shRNA and sorted for GFP expression. Western blot analysis confirmed knockdown of neurofibromin. (B) Early passage endothelial cells were infected with a pTRIPZ lentiviral vector expressing either a non-silencing control or a NF1 miR-based shRNA. The infected cells were induced with 1 µg/mL doxycycline for 48 h to induce expression of the microRNA along with red fluorescent protein (expressed in tandem) and sorted for RFP expression. Western blot confirms knockdown of neurofibromin in the presence of doxycycline while no knockdown was seen in the uninduced cells. (C) Uninduced (−Dox) and induced (+Dox) endothelial cells were serum starved for 24 h and levels of active Ras (RasGTP) were determined by using GST-Raf pull-down assay (Pierce), according to the manufacturer’s protocol. Total Ras in the total cell lysates confirmed similar amounts of protein input and was used to normalize GTP-Ras quantification. (D) Cells were treated as described above but western analysis was performed to measure activation of several key signaling proteins including phospho-Akt, phospho-S6, and phospho-ERK. Equal lysate loading was confirmed by monitoring total ERK2 levels. All experiments were performed in at least three independent sets of control and NF1 knockdown HUVECs and quantification results were averaged. (Identical results were seen with both knockdown vectors (see for example figure 6 ) Error bars represent standard error of the mean (**p

    Journal: PLoS ONE

    Article Title: Loss of NF1 Expression in Human Endothelial Cells Promotes Autonomous Proliferation and Altered Vascular Morphogenesis

    doi: 10.1371/journal.pone.0049222

    Figure Lengend Snippet: Knockdown of NF1 in Human Endothelial Cells is Sufficient to Activate Ras and Induce Cellular Signaling. (A) Early passage HUVECs were infected with a pSIREN-GFP retroviral vector carrying either a control or a NF1 shRNA and sorted for GFP expression. Western blot analysis confirmed knockdown of neurofibromin. (B) Early passage endothelial cells were infected with a pTRIPZ lentiviral vector expressing either a non-silencing control or a NF1 miR-based shRNA. The infected cells were induced with 1 µg/mL doxycycline for 48 h to induce expression of the microRNA along with red fluorescent protein (expressed in tandem) and sorted for RFP expression. Western blot confirms knockdown of neurofibromin in the presence of doxycycline while no knockdown was seen in the uninduced cells. (C) Uninduced (−Dox) and induced (+Dox) endothelial cells were serum starved for 24 h and levels of active Ras (RasGTP) were determined by using GST-Raf pull-down assay (Pierce), according to the manufacturer’s protocol. Total Ras in the total cell lysates confirmed similar amounts of protein input and was used to normalize GTP-Ras quantification. (D) Cells were treated as described above but western analysis was performed to measure activation of several key signaling proteins including phospho-Akt, phospho-S6, and phospho-ERK. Equal lysate loading was confirmed by monitoring total ERK2 levels. All experiments were performed in at least three independent sets of control and NF1 knockdown HUVECs and quantification results were averaged. (Identical results were seen with both knockdown vectors (see for example figure 6 ) Error bars represent standard error of the mean (**p

    Article Snippet: Inducible knockdown of NF1 was achieved using a lentiviral pTRIPZ vector from Open Biosystems carrying the V2THS_260806 sequence for knocking down NF1.

    Techniques: Infection, Plasmid Preparation, shRNA, Expressing, Western Blot, Pull Down Assay, Activation Assay