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  • 99
    New England Biolabs kpni
    Kpni, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3744 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher high fidelity pcr
    Validation of <t>PsnSuSy2</t> integration into tobacco genome and expression in transgenic tobacco lines. a. Validation of PsnSuSy2 tobacco transgenic lines using <t>PCR</t> amplification. Genomic DNA was extracted from the leaves of one-month-old transgenic tobacco. The PCR products were electrophoresed on a 1.2% agarose gel with DL15000 DNA marker (first lane). b. Relative expression of PsnSuSy2 in two month-old stem segments of plastichron index (PI) 3–5 determined by real-time RT-PCR. C. SuSy enzyme activity of in two month-old stem segments of PI3–5. Expressed levels in both Fig. 3b and 3c were averaged from five different samples per line ± S.E. The t -test was used to examine the significance of difference between PsnSusy2 transgenic and wildtype lines, and *denotes significance at p
    High Fidelity Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 776 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bamhi
    Validation of <t>PsnSuSy2</t> integration into tobacco genome and expression in transgenic tobacco lines. a. Validation of PsnSuSy2 tobacco transgenic lines using <t>PCR</t> amplification. Genomic DNA was extracted from the leaves of one-month-old transgenic tobacco. The PCR products were electrophoresed on a 1.2% agarose gel with DL15000 DNA marker (first lane). b. Relative expression of PsnSuSy2 in two month-old stem segments of plastichron index (PI) 3–5 determined by real-time RT-PCR. C. SuSy enzyme activity of in two month-old stem segments of PI3–5. Expressed levels in both Fig. 3b and 3c were averaged from five different samples per line ± S.E. The t -test was used to examine the significance of difference between PsnSusy2 transgenic and wildtype lines, and *denotes significance at p
    Bamhi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 11236 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t4 ligase
    Validation of <t>PsnSuSy2</t> integration into tobacco genome and expression in transgenic tobacco lines. a. Validation of PsnSuSy2 tobacco transgenic lines using <t>PCR</t> amplification. Genomic DNA was extracted from the leaves of one-month-old transgenic tobacco. The PCR products were electrophoresed on a 1.2% agarose gel with DL15000 DNA marker (first lane). b. Relative expression of PsnSuSy2 in two month-old stem segments of plastichron index (PI) 3–5 determined by real-time RT-PCR. C. SuSy enzyme activity of in two month-old stem segments of PI3–5. Expressed levels in both Fig. 3b and 3c were averaged from five different samples per line ± S.E. The t -test was used to examine the significance of difference between PsnSusy2 transgenic and wildtype lines, and *denotes significance at p
    T4 Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9635 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Biotechnology Information phylogenetic sequence analysis
    Validation of <t>PsnSuSy2</t> integration into tobacco genome and expression in transgenic tobacco lines. a. Validation of PsnSuSy2 tobacco transgenic lines using <t>PCR</t> amplification. Genomic DNA was extracted from the leaves of one-month-old transgenic tobacco. The PCR products were electrophoresed on a 1.2% agarose gel with DL15000 DNA marker (first lane). b. Relative expression of PsnSuSy2 in two month-old stem segments of plastichron index (PI) 3–5 determined by real-time RT-PCR. C. SuSy enzyme activity of in two month-old stem segments of PI3–5. Expressed levels in both Fig. 3b and 3c were averaged from five different samples per line ± S.E. The t -test was used to examine the significance of difference between PsnSusy2 transgenic and wildtype lines, and *denotes significance at p
    Phylogenetic Sequence Analysis, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 89/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pmd18 t cloning vector
    Validation of <t>PsnSuSy2</t> integration into tobacco genome and expression in transgenic tobacco lines. a. Validation of PsnSuSy2 tobacco transgenic lines using <t>PCR</t> amplification. Genomic DNA was extracted from the leaves of one-month-old transgenic tobacco. The PCR products were electrophoresed on a 1.2% agarose gel with DL15000 DNA marker (first lane). b. Relative expression of PsnSuSy2 in two month-old stem segments of plastichron index (PI) 3–5 determined by real-time RT-PCR. C. SuSy enzyme activity of in two month-old stem segments of PI3–5. Expressed levels in both Fig. 3b and 3c were averaged from five different samples per line ± S.E. The t -test was used to examine the significance of difference between PsnSusy2 transgenic and wildtype lines, and *denotes significance at p
    Pmd18 T Cloning Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biotechnology Information genbank
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
    Genbank, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 94/100, based on 6786 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa primescript rt reagent kit
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
    Primescript Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 72315 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher superscript ii reverse transcriptase
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
    Superscript Ii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 90426 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    superscript ii reverse transcriptase - by Bioz Stars, 2020-08
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    99
    Thermo Fisher 7500 real time pcr system
    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from <t>Genbank</t> ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.
    7500 Real Time Pcr System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 54115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Validation of PsnSuSy2 integration into tobacco genome and expression in transgenic tobacco lines. a. Validation of PsnSuSy2 tobacco transgenic lines using PCR amplification. Genomic DNA was extracted from the leaves of one-month-old transgenic tobacco. The PCR products were electrophoresed on a 1.2% agarose gel with DL15000 DNA marker (first lane). b. Relative expression of PsnSuSy2 in two month-old stem segments of plastichron index (PI) 3–5 determined by real-time RT-PCR. C. SuSy enzyme activity of in two month-old stem segments of PI3–5. Expressed levels in both Fig. 3b and 3c were averaged from five different samples per line ± S.E. The t -test was used to examine the significance of difference between PsnSusy2 transgenic and wildtype lines, and *denotes significance at p

    Journal: PLoS ONE

    Article Title: Overexpression of Poplar Xylem Sucrose Synthase in Tobacco Leads to a Thickened Cell Wall and Increased Height

    doi: 10.1371/journal.pone.0120669

    Figure Lengend Snippet: Validation of PsnSuSy2 integration into tobacco genome and expression in transgenic tobacco lines. a. Validation of PsnSuSy2 tobacco transgenic lines using PCR amplification. Genomic DNA was extracted from the leaves of one-month-old transgenic tobacco. The PCR products were electrophoresed on a 1.2% agarose gel with DL15000 DNA marker (first lane). b. Relative expression of PsnSuSy2 in two month-old stem segments of plastichron index (PI) 3–5 determined by real-time RT-PCR. C. SuSy enzyme activity of in two month-old stem segments of PI3–5. Expressed levels in both Fig. 3b and 3c were averaged from five different samples per line ± S.E. The t -test was used to examine the significance of difference between PsnSusy2 transgenic and wildtype lines, and *denotes significance at p

    Article Snippet: The full-length coding region of PsnSuSy2 was obtained by high-fidelity PCR (Thermo Scientific) with two specific primers, SuSy2 -F ( 5`-ATGTCTGTACTTACTCGTGTCC-3` ) and SuSy2 -R ( 5`-TTACTCGATAGTCAAAGGAACAG-3` ) and cloned the PCR products into the pMD18-T cloning vector (TaKaRa Biotechnology, Dalian, China).

    Techniques: Expressing, Transgenic Assay, Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Marker, Quantitative RT-PCR, Activity Assay

    Tissue-specific expression of the PsnSuSy2 in one-year-old hybrid Populus simonii × Populus nigra propagated from two years old hybrid trees. a. Tissue-specific expression pattern characterized by RT-PCR. b. Tissue-specific expression pattern characterized by real-time quantitative PCR. The expression levels for real-time quantitative PCR were averaged from three replicates.

    Journal: PLoS ONE

    Article Title: Overexpression of Poplar Xylem Sucrose Synthase in Tobacco Leads to a Thickened Cell Wall and Increased Height

    doi: 10.1371/journal.pone.0120669

    Figure Lengend Snippet: Tissue-specific expression of the PsnSuSy2 in one-year-old hybrid Populus simonii × Populus nigra propagated from two years old hybrid trees. a. Tissue-specific expression pattern characterized by RT-PCR. b. Tissue-specific expression pattern characterized by real-time quantitative PCR. The expression levels for real-time quantitative PCR were averaged from three replicates.

    Article Snippet: The full-length coding region of PsnSuSy2 was obtained by high-fidelity PCR (Thermo Scientific) with two specific primers, SuSy2 -F ( 5`-ATGTCTGTACTTACTCGTGTCC-3` ) and SuSy2 -R ( 5`-TTACTCGATAGTCAAAGGAACAG-3` ) and cloned the PCR products into the pMD18-T cloning vector (TaKaRa Biotechnology, Dalian, China).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

    Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from Genbank ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.

    Journal: PLoS ONE

    Article Title: Overexpression of Poplar Xylem Sucrose Synthase in Tobacco Leads to a Thickened Cell Wall and Increased Height

    doi: 10.1371/journal.pone.0120669

    Figure Lengend Snippet: Phylogenetic tree of selected full SuSy coding sequences derived using the neighbor-joining method. The sequences were obtained by translating the following 116 full-length cDNA sequences of SuSy isozymes downloaded from Genbank ( http://www.ncbi.nlm.nih.gov/genbank ): Arabidopsis thaliana (AtSuSy1–6) isozyme1–6 (At5g20830, At5g 49190, At4g02280, At3g43190, At5g37180, At1g73370), Actinidia chinensis (AcSuSy) isozyme (AFO84090.1,), Actinidia deliciosa (AdSuSy) isozyme (AFO84091.1), Amorphophallus konjac (AkSuSy1) isozyme (AEH27530.1), Arachis hypogaea (AhSuSy2) isozyme (AFS17278.1), Arachis hypogaea var.vulgaris (AhSuSy) isozyme (AEF56625.1), Bambusa oldhamii (BoSuSy1–4) isozyme1–4(AAV64256.2, AAL50571.1, AAL50570.1, AAL50572.2), Betula luminifera (BlSuSy1–3) isozyme 1–3(AAV64256.2,AGV22112.1, AGV22113.1), Beta vulgaris (BvSuSy1) isozyme1 (ABR87939.1), Camellia sinensi s (CasSuSy1–2) isozyme1–2 (AHL29281.1, AHL29282.1), Chenopodium rubrum ( Cr SuSy) isozyme (CAA57881.1), Cicer arietinum ( Cia SuSy) isozyme (AEE60913.1,), Citrus unshiu (CuSuSy) isozyme(BAA88904.1), Citrullus lanatus (ClSuSy) isozyme (BAA89232.1), Coccomyxa sub ellipsoidea C-169(CeSuSy) isozyme (XP_005643464.1), Coffea arabica (CaSuSy) isozyme (CAJ32597.1), Coffea canephora (CcSuSy) isozyme (ABI17891.1), Craterostigma plantagineum (CpSuSy1–2) isozyme1–2 (CAB38021.1, CAB38022.1), Cucumis sativus (CsSuSy1,3–5) isozyme1,3–5 (AGA95977.1 AEN83999.1 AGA95976.1,P_001267613.1), Daucus carota (DcSuSy1,2) isozyme1,2 (CAA53081.1, CAA76057.1), Dianthus caryophyllus (DicSuSy) isozyme (BAJ10424.1), Dendrobium officinale (DoSuSy) isozyme (ADY02961.1), Eucalyptus grandis (EgSuSy1,3) isozyme1,3(ABB53601.1, ABB53602.1), Gossypium arboreum (GaSuSy1–6) isozyme1–6(AEV40460.1, AEV40461.1, AEV40462.1, AEV40463.1, AEV40464.1, AEV40465.1), Gossypium hirsutum (GhSuSy) isozyme (AIE38018.1), Gunnera manicata (GmSuSy)SuSy isozyme (ADP88918.1), Hevea brasiliensis (HbSuSy1–6) isozyme1–6(AGM14946.1, AGM14947.1, AGM14948, AGM14949.1, AGM14950.1, AGM14951. 1), Ipomoea batatas (IbSuSy) isozyme (ACL00957.1), Jatropha curcas (JcSuSy) isozyme (AGH29112.1), Lilium davidii (LidSuSy) isozyme (AGW23638.1), Lilium davidii var. unicolor(LdSuSy1,2) isozyme1,2(AHM02468.1, AHN50409.1), Litchi chinensis (LcSuSy) isozyme (AFP23359.1), Lolium perenne (LpSuSy) isozyme (BAE79815.1), Manihot esculenta (MeSuSy) isozyme (ABD96570.1), Manihot esculenta (MeSuSy1,4) isozyme1,4 (AIJ28962.1, AIJ28961.1), Manihot esculenta subsp . Flabellifolia (MefSuSy1,4) isozyme1,4 (AIJ28960.1,AIJ28959.1), Malus domestica (MdSuSy) isozyme (AFU56881.1), Medicago sativa (MsSuSy) isozyme (AAC17867.1), Medicago truncatula (MtSuSy) isozyme (XP_003616166.1), Musa acuminata (MaSuSy) isozyme (AEO09338.2), Nicotiana tabacum (NtSuSy) isozyme (AHL84158.1), Oncidium hybrid (OhSuSy, OhSuSy1) isozyme,1(AAM95943.1, AEA76429.1), Orobanche ramose (OrSuSy1) isozyme1 (AEN79500.1), Oryza sativa (OsSuSy1,3–7) isozyme1,3–7(|AEX32874.1, AEX32876.1, AEX32876.1, AEX32877.1, AEX32878.1, AEX32880.1), Oryza sativa (OsSuSy2–1, OsSuSy2–2) isozyme2–1,2–2 (AEX32875.1, AFI71274.1), Populus simonii × Populus nigra (PsnSuSy2) isozyme2, Populus trichocarpa (PtSuSy1–7) isozyme1–7(ADV71183.1, ADV71184.1, ADV71185.1, ADV71186.1, ADV71187.1, ADV71188.1, ADV71189.1), Populus deltoids (PdSuSy) isozyme (AHA41509.1), Populus tremuloides (PtSuSy) isozyme (AAR03498.1), Potamogeton distinctus (PodSuSy1–2) isozyme1–2(BAE06058.1, BAE06059.1), Phaseolus vulgaris (PvSuSy) isozyme (AAN76498.1), Pinus taeda (PitSuSy) isozyme (ABR15470.1), Prunus serrulata (PsSuSy) isozyme (AIL23782.1), Prunus persica SuSy (PpSuSy2,4–6) isozyme 2,4–6(AHZ90138.1, AHZ90140.1, AHZ90141.1, AHZ90142.1), Prunus persica (PpSuSy) isozyme(AHZ90138.1), Solanum tuberosum (SotSuSy) isozyme (AAA33841.1), Solanum tuberosum (StSuSy, StSuSy2) isozyme,2 (AAO67719.1, AAO34668.1), Solanum lycopersicum (SlSuSy) isozyme (AAA34196.1), Saccharum officinarum (SoSuSy) isozyme (AGI56230.1), Theobroma cacao (TcSuSy-1, TcSuSy-2) isozyme-1,-2 (XP_007032183.1, XP_007032184.1), Theobroma cacao (TcSuSy2–2) isozyme 2–2 (XP_007035652.1), Theobroma cacao (TcSuSy3–2) isozyme 3–2 (XP_007050985.1), Theobroma cacao (TcSuSy5–4) isozyme 5–4 (XP_007032186.1), Theobroma cacao (TcSuSy6–1) isozyme 6–1 (XP_007037101.1), Triticum polonicum (TpSuSy2–2) isozyme2–2 (AIL88517.1), Triticum polonicum (TpSuSy3) isozyme (AIL88515.1), Vigna radiata (VrSuSy) isozyme (BAA01108.1), Vigna angularis (VaSuSy) isozyme (BAH56282.1). The tree was generated using MEGA, version 4, with bootstrap of 1,000.

    Article Snippet: Phylogenetic sequence analysis A total of 116 putative plant SuSy2 protein sequences from both dicotyledon and monocotyledon plants were retrieved from GenBank at the National Center for Biotechnology Information ( http://www.ncbi.nlm.nih.gov/genbank ) and aligned with PsnSuSy2 using ClustalW within the MEGA 4.0 software package [ ].

    Techniques: Derivative Assay, Generated