psilencer 4.1-cmv vector Search Results


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  • 90
    Thermo Fisher psilencer 4 1 cmv vector
    Psilencer 4 1 Cmv Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher psilencer 4 1 cmv puro vector
    Psilencer 4 1 Cmv Puro Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher psilencer 4 1 cmv hygro vector
    Psilencer 4 1 Cmv Hygro Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher psilencer 4 1 cmv vectors
    Psilencer 4 1 Cmv Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher psilencer 4 1 cmv neo vector kit
    Psilencer 4 1 Cmv Neo Vector Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Thermo Fisher psilencer 4 1 cmv puro vectors
    Psilencer 4 1 Cmv Puro Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher psilencer 4 1 cmv neo expression vector
    Psilencer 4 1 Cmv Neo Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher psilencer 4 1 cmv puro dna vector
    Psilencer 4 1 Cmv Puro Dna Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher psilencer 4 1 cmv puro expression vector
    Psilencer 4 1 Cmv Puro Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher psilencer 4 1 cmv puro mammalian expression vector
    miR-199a-3p suppressed RA-FLS proliferation ( A ) RT-qPCR of miR-199a-3p in RA-FLSs transfected with pSilencer 4.1-miR-199a-3p plasmid (miR-199a-3p) or no-insert control pSilencer <t>4.1-CMV</t> <t>puro</t> plasmid (Mock). ( B ) Forty-eight hours post transfection, MTT assay was performed to test the viability of miR-199a-3p or Mock RA-FLSs. ( C , D ). EdU assay of miR-199a-3p or Mock transfected RA-FLSs 48 h post transfection. Cells were stained for EdU and Hoechst (to mark nuclei) (C), and quantitated for EdU+ cell percentage (D). CCK8 assay showed that ectopic expression of miR-199a-3p significantly inhibited RA-FLS proliferation rate over 5 days. n =4 or n =6 (A,D); * P
    Psilencer 4 1 Cmv Puro Mammalian Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher psilencer 4 1 cmv puro expression vectors
    miR-199a-3p suppressed RA-FLS proliferation ( A ) RT-qPCR of miR-199a-3p in RA-FLSs transfected with pSilencer 4.1-miR-199a-3p plasmid (miR-199a-3p) or no-insert control pSilencer <t>4.1-CMV</t> <t>puro</t> plasmid (Mock). ( B ) Forty-eight hours post transfection, MTT assay was performed to test the viability of miR-199a-3p or Mock RA-FLSs. ( C , D ). EdU assay of miR-199a-3p or Mock transfected RA-FLSs 48 h post transfection. Cells were stained for EdU and Hoechst (to mark nuclei) (C), and quantitated for EdU+ cell percentage (D). CCK8 assay showed that ectopic expression of miR-199a-3p significantly inhibited RA-FLS proliferation rate over 5 days. n =4 or n =6 (A,D); * P
    Psilencer 4 1 Cmv Puro Expression Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher psilencer 4 1 cmv hyg vector
    miR-199a-3p suppressed RA-FLS proliferation ( A ) RT-qPCR of miR-199a-3p in RA-FLSs transfected with pSilencer 4.1-miR-199a-3p plasmid (miR-199a-3p) or no-insert control pSilencer <t>4.1-CMV</t> <t>puro</t> plasmid (Mock). ( B ) Forty-eight hours post transfection, MTT assay was performed to test the viability of miR-199a-3p or Mock RA-FLSs. ( C , D ). EdU assay of miR-199a-3p or Mock transfected RA-FLSs 48 h post transfection. Cells were stained for EdU and Hoechst (to mark nuclei) (C), and quantitated for EdU+ cell percentage (D). CCK8 assay showed that ectopic expression of miR-199a-3p significantly inhibited RA-FLS proliferation rate over 5 days. n =4 or n =6 (A,D); * P
    Psilencer 4 1 Cmv Hyg Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher psilencer 4 1 cmv hygro shrna expression vector
    miR-199a-3p suppressed RA-FLS proliferation ( A ) RT-qPCR of miR-199a-3p in RA-FLSs transfected with pSilencer 4.1-miR-199a-3p plasmid (miR-199a-3p) or no-insert control pSilencer <t>4.1-CMV</t> <t>puro</t> plasmid (Mock). ( B ) Forty-eight hours post transfection, MTT assay was performed to test the viability of miR-199a-3p or Mock RA-FLSs. ( C , D ). EdU assay of miR-199a-3p or Mock transfected RA-FLSs 48 h post transfection. Cells were stained for EdU and Hoechst (to mark nuclei) (C), and quantitated for EdU+ cell percentage (D). CCK8 assay showed that ectopic expression of miR-199a-3p significantly inhibited RA-FLS proliferation rate over 5 days. n =4 or n =6 (A,D); * P
    Psilencer 4 1 Cmv Hygro Shrna Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher psilencer 4 1 cmv neo sirna expression vector
    miR-199a-3p suppressed RA-FLS proliferation ( A ) RT-qPCR of miR-199a-3p in RA-FLSs transfected with pSilencer 4.1-miR-199a-3p plasmid (miR-199a-3p) or no-insert control pSilencer <t>4.1-CMV</t> <t>puro</t> plasmid (Mock). ( B ) Forty-eight hours post transfection, MTT assay was performed to test the viability of miR-199a-3p or Mock RA-FLSs. ( C , D ). EdU assay of miR-199a-3p or Mock transfected RA-FLSs 48 h post transfection. Cells were stained for EdU and Hoechst (to mark nuclei) (C), and quantitated for EdU+ cell percentage (D). CCK8 assay showed that ectopic expression of miR-199a-3p significantly inhibited RA-FLS proliferation rate over 5 days. n =4 or n =6 (A,D); * P
    Psilencer 4 1 Cmv Neo Sirna Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher control psilencer 4 1 cmv neo vector
    miR-199a-3p suppressed RA-FLS proliferation ( A ) RT-qPCR of miR-199a-3p in RA-FLSs transfected with pSilencer 4.1-miR-199a-3p plasmid (miR-199a-3p) or no-insert control pSilencer <t>4.1-CMV</t> <t>puro</t> plasmid (Mock). ( B ) Forty-eight hours post transfection, MTT assay was performed to test the viability of miR-199a-3p or Mock RA-FLSs. ( C , D ). EdU assay of miR-199a-3p or Mock transfected RA-FLSs 48 h post transfection. Cells were stained for EdU and Hoechst (to mark nuclei) (C), and quantitated for EdU+ cell percentage (D). CCK8 assay showed that ectopic expression of miR-199a-3p significantly inhibited RA-FLS proliferation rate over 5 days. n =4 or n =6 (A,D); * P
    Control Psilencer 4 1 Cmv Neo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher psilencer 4 1 cmv expression vectors
    miR-199a-3p suppressed RA-FLS proliferation ( A ) RT-qPCR of miR-199a-3p in RA-FLSs transfected with pSilencer 4.1-miR-199a-3p plasmid (miR-199a-3p) or no-insert control pSilencer <t>4.1-CMV</t> <t>puro</t> plasmid (Mock). ( B ) Forty-eight hours post transfection, MTT assay was performed to test the viability of miR-199a-3p or Mock RA-FLSs. ( C , D ). EdU assay of miR-199a-3p or Mock transfected RA-FLSs 48 h post transfection. Cells were stained for EdU and Hoechst (to mark nuclei) (C), and quantitated for EdU+ cell percentage (D). CCK8 assay showed that ectopic expression of miR-199a-3p significantly inhibited RA-FLS proliferation rate over 5 days. n =4 or n =6 (A,D); * P
    Psilencer 4 1 Cmv Expression Vectors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psilencer 4 1 cmv expression vectors/product/Thermo Fisher
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    86
    Thermo Fisher bam hi hind iii digested expression vector psilencer 4 1 cmv hygro plasmid
    miR-199a-3p suppressed RA-FLS proliferation ( A ) RT-qPCR of miR-199a-3p in RA-FLSs transfected with pSilencer 4.1-miR-199a-3p plasmid (miR-199a-3p) or no-insert control pSilencer <t>4.1-CMV</t> <t>puro</t> plasmid (Mock). ( B ) Forty-eight hours post transfection, MTT assay was performed to test the viability of miR-199a-3p or Mock RA-FLSs. ( C , D ). EdU assay of miR-199a-3p or Mock transfected RA-FLSs 48 h post transfection. Cells were stained for EdU and Hoechst (to mark nuclei) (C), and quantitated for EdU+ cell percentage (D). CCK8 assay showed that ectopic expression of miR-199a-3p significantly inhibited RA-FLS proliferation rate over 5 days. n =4 or n =6 (A,D); * P
    Bam Hi Hind Iii Digested Expression Vector Psilencer 4 1 Cmv Hygro Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    miR-199a-3p suppressed RA-FLS proliferation ( A ) RT-qPCR of miR-199a-3p in RA-FLSs transfected with pSilencer 4.1-miR-199a-3p plasmid (miR-199a-3p) or no-insert control pSilencer 4.1-CMV puro plasmid (Mock). ( B ) Forty-eight hours post transfection, MTT assay was performed to test the viability of miR-199a-3p or Mock RA-FLSs. ( C , D ). EdU assay of miR-199a-3p or Mock transfected RA-FLSs 48 h post transfection. Cells were stained for EdU and Hoechst (to mark nuclei) (C), and quantitated for EdU+ cell percentage (D). CCK8 assay showed that ectopic expression of miR-199a-3p significantly inhibited RA-FLS proliferation rate over 5 days. n =4 or n =6 (A,D); * P

    Journal: Bioscience Reports

    Article Title: MiR-199a-3p inhibits proliferation and induces apoptosis in rheumatoid arthritis fibroblast-like synoviocytes via suppressing retinoblastoma 1

    doi: 10.1042/BSR20180982

    Figure Lengend Snippet: miR-199a-3p suppressed RA-FLS proliferation ( A ) RT-qPCR of miR-199a-3p in RA-FLSs transfected with pSilencer 4.1-miR-199a-3p plasmid (miR-199a-3p) or no-insert control pSilencer 4.1-CMV puro plasmid (Mock). ( B ) Forty-eight hours post transfection, MTT assay was performed to test the viability of miR-199a-3p or Mock RA-FLSs. ( C , D ). EdU assay of miR-199a-3p or Mock transfected RA-FLSs 48 h post transfection. Cells were stained for EdU and Hoechst (to mark nuclei) (C), and quantitated for EdU+ cell percentage (D). CCK8 assay showed that ectopic expression of miR-199a-3p significantly inhibited RA-FLS proliferation rate over 5 days. n =4 or n =6 (A,D); * P

    Article Snippet: DNA constructs and siRNA To generate the miR-199a-3p overexpressing construct, a DNA fragment containing human miR-199a-3p precursor was amplified by PCR from human genomic DNA and inserted into a pSilencer 4.1-CMV puro mammalian expression vector (Thermo Fisher Scientific, Waltham, MA, U.S.A.).

    Techniques: Quantitative RT-PCR, Transfection, Plasmid Preparation, MTT Assay, EdU Assay, Staining, CCK-8 Assay, Expressing

    RB1 is a direct target of miR-199a-3p ( A ) TargetScan analysis showing the WT 3′-UTR of RB1 mRNA containing a putative miR-199a-3p target site. A mutant (MUT) sequence was designed accordingly to be tested for luciferase assay together with the WT. ( B ) Luciferase reporter assay comparing WT with MUT RB1 3′-UTR targetting by miR-199a-3p. RA-FLSs cells were co-transfected with RB1 3′-UTR firefly luciferase reporter constructs harboring WT or MUT miR-199a-3p-targetting sequences and an miR-199a-3p-expressing plasmid (miR-199a-3p) or a pSliencer 4.1-CMV puro vector (miR-control). Firefly luciferase activity was normalized to Renilla luciferase activity. ( C ) RT-qPCR analysis of RB1 mRNA in RA-FLSs transfected with miR-199a-3p or miR-control. ( D ) RB1 protein expression in RA-FLSs transfected with miR-199a-3p or miR-control. n =5; * P

    Journal: Bioscience Reports

    Article Title: MiR-199a-3p inhibits proliferation and induces apoptosis in rheumatoid arthritis fibroblast-like synoviocytes via suppressing retinoblastoma 1

    doi: 10.1042/BSR20180982

    Figure Lengend Snippet: RB1 is a direct target of miR-199a-3p ( A ) TargetScan analysis showing the WT 3′-UTR of RB1 mRNA containing a putative miR-199a-3p target site. A mutant (MUT) sequence was designed accordingly to be tested for luciferase assay together with the WT. ( B ) Luciferase reporter assay comparing WT with MUT RB1 3′-UTR targetting by miR-199a-3p. RA-FLSs cells were co-transfected with RB1 3′-UTR firefly luciferase reporter constructs harboring WT or MUT miR-199a-3p-targetting sequences and an miR-199a-3p-expressing plasmid (miR-199a-3p) or a pSliencer 4.1-CMV puro vector (miR-control). Firefly luciferase activity was normalized to Renilla luciferase activity. ( C ) RT-qPCR analysis of RB1 mRNA in RA-FLSs transfected with miR-199a-3p or miR-control. ( D ) RB1 protein expression in RA-FLSs transfected with miR-199a-3p or miR-control. n =5; * P

    Article Snippet: DNA constructs and siRNA To generate the miR-199a-3p overexpressing construct, a DNA fragment containing human miR-199a-3p precursor was amplified by PCR from human genomic DNA and inserted into a pSilencer 4.1-CMV puro mammalian expression vector (Thermo Fisher Scientific, Waltham, MA, U.S.A.).

    Techniques: Mutagenesis, Sequencing, Luciferase, Reporter Assay, Transfection, Construct, Expressing, Plasmid Preparation, Activity Assay, Quantitative RT-PCR

    miR-199a-3p regulated RA-FLS proliferation and apoptosis partially via suppressing RB1 RA-FLS was transfected with an miR-199a-3p-expressing plasmid (miR-199a-3p) or a pSliencer 4.1-CMV puro vector (miR-control) or miR-199a-3p in combination with an RB1-overexpression plasmid (miR + pcDNA-RB1) or miR-199a-3p in combination with a negative control plasmid (miR + pcDNA-NC). ( A ) Western blot of RB1 protein in RA-FLSs transfected with negative control (NC) or pcDNA3-RB1. ( B ) Proliferation of transfected RA-FLS was measured by the MTT assay. ( C ) Apoptosis was measured by FITC Annexin V and PI staining followed by flow cytometry. ( D ) Caspase-3 activity was measured by a colorimetric method. n =6; * P

    Journal: Bioscience Reports

    Article Title: MiR-199a-3p inhibits proliferation and induces apoptosis in rheumatoid arthritis fibroblast-like synoviocytes via suppressing retinoblastoma 1

    doi: 10.1042/BSR20180982

    Figure Lengend Snippet: miR-199a-3p regulated RA-FLS proliferation and apoptosis partially via suppressing RB1 RA-FLS was transfected with an miR-199a-3p-expressing plasmid (miR-199a-3p) or a pSliencer 4.1-CMV puro vector (miR-control) or miR-199a-3p in combination with an RB1-overexpression plasmid (miR + pcDNA-RB1) or miR-199a-3p in combination with a negative control plasmid (miR + pcDNA-NC). ( A ) Western blot of RB1 protein in RA-FLSs transfected with negative control (NC) or pcDNA3-RB1. ( B ) Proliferation of transfected RA-FLS was measured by the MTT assay. ( C ) Apoptosis was measured by FITC Annexin V and PI staining followed by flow cytometry. ( D ) Caspase-3 activity was measured by a colorimetric method. n =6; * P

    Article Snippet: DNA constructs and siRNA To generate the miR-199a-3p overexpressing construct, a DNA fragment containing human miR-199a-3p precursor was amplified by PCR from human genomic DNA and inserted into a pSilencer 4.1-CMV puro mammalian expression vector (Thermo Fisher Scientific, Waltham, MA, U.S.A.).

    Techniques: Transfection, Expressing, Plasmid Preparation, Over Expression, Negative Control, Western Blot, MTT Assay, Staining, Flow Cytometry, Cytometry, Activity Assay