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  • 88
    Millipore pseudovirus particles
    Effect of membrane-active compounds on HIV membrane lateral packing (left panels) and <t>pseudovirus</t> cell entry (right panels). ( a ) Left: Samples were incubated with M β CD (1 mM) for 15 minutes before collecting GP images from Laurdan-labeled individual GUVs. The group of vesicles that displayed better fitting to two-component distributions is plotted using two boxes, the upper one corresponding to the more ordered domains and the lower one to the more disordered regions. Right: HIV-1 pseudoviruses were pre-attached to lysine-coated plates 57 and treated with increasing concentrations of M β CD. After washing, reporter TZM-bl cells were layered on top, and entry (gene transduction) inferred from the number of total cells expressing GFP, as described previously 39 . Values represent means ± SD of three independent assays. ( b ) Samples were treated with CpreTM peptide. Average GP values were determined for GUVs treated for at least 15 minutes with 1 μM peptide (left). The population of GUVs displaying membrane diaphragms were sorted out and measured separately. ( c ) Samples incubated with AAPH. GP values were determined after 15 minutes incubation with 20 mM AAPH (left). Conditions otherwise as in the previous panels. (*** p
    Pseudovirus Particles, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore pseudoviral particles encoding shrnas
    Effect of membrane-active compounds on HIV membrane lateral packing (left panels) and <t>pseudovirus</t> cell entry (right panels). ( a ) Left: Samples were incubated with M β CD (1 mM) for 15 minutes before collecting GP images from Laurdan-labeled individual GUVs. The group of vesicles that displayed better fitting to two-component distributions is plotted using two boxes, the upper one corresponding to the more ordered domains and the lower one to the more disordered regions. Right: HIV-1 pseudoviruses were pre-attached to lysine-coated plates 57 and treated with increasing concentrations of M β CD. After washing, reporter TZM-bl cells were layered on top, and entry (gene transduction) inferred from the number of total cells expressing GFP, as described previously 39 . Values represent means ± SD of three independent assays. ( b ) Samples were treated with CpreTM peptide. Average GP values were determined for GUVs treated for at least 15 minutes with 1 μM peptide (left). The population of GUVs displaying membrane diaphragms were sorted out and measured separately. ( c ) Samples incubated with AAPH. GP values were determined after 15 minutes incubation with 20 mM AAPH (left). Conditions otherwise as in the previous panels. (*** p
    Pseudoviral Particles Encoding Shrnas, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pseudoviral particles encoding shrnas/product/Millipore
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    pseudoviral particles encoding shrnas - by Bioz Stars, 2020-08
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    85
    System Biosciences Inc transduction ready pseudoviral particles
    Effect of membrane-active compounds on HIV membrane lateral packing (left panels) and <t>pseudovirus</t> cell entry (right panels). ( a ) Left: Samples were incubated with M β CD (1 mM) for 15 minutes before collecting GP images from Laurdan-labeled individual GUVs. The group of vesicles that displayed better fitting to two-component distributions is plotted using two boxes, the upper one corresponding to the more ordered domains and the lower one to the more disordered regions. Right: HIV-1 pseudoviruses were pre-attached to lysine-coated plates 57 and treated with increasing concentrations of M β CD. After washing, reporter TZM-bl cells were layered on top, and entry (gene transduction) inferred from the number of total cells expressing GFP, as described previously 39 . Values represent means ± SD of three independent assays. ( b ) Samples were treated with CpreTM peptide. Average GP values were determined for GUVs treated for at least 15 minutes with 1 μM peptide (left). The population of GUVs displaying membrane diaphragms were sorted out and measured separately. ( c ) Samples incubated with AAPH. GP values were determined after 15 minutes incubation with 20 mM AAPH (left). Conditions otherwise as in the previous panels. (*** p
    Transduction Ready Pseudoviral Particles, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transduction ready pseudoviral particles/product/System Biosciences Inc
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    85
    Syntaxin infectious pseudoviral particles
    Effect of membrane-active compounds on HIV membrane lateral packing (left panels) and <t>pseudovirus</t> cell entry (right panels). ( a ) Left: Samples were incubated with M β CD (1 mM) for 15 minutes before collecting GP images from Laurdan-labeled individual GUVs. The group of vesicles that displayed better fitting to two-component distributions is plotted using two boxes, the upper one corresponding to the more ordered domains and the lower one to the more disordered regions. Right: HIV-1 pseudoviruses were pre-attached to lysine-coated plates 57 and treated with increasing concentrations of M β CD. After washing, reporter TZM-bl cells were layered on top, and entry (gene transduction) inferred from the number of total cells expressing GFP, as described previously 39 . Values represent means ± SD of three independent assays. ( b ) Samples were treated with CpreTM peptide. Average GP values were determined for GUVs treated for at least 15 minutes with 1 μM peptide (left). The population of GUVs displaying membrane diaphragms were sorted out and measured separately. ( c ) Samples incubated with AAPH. GP values were determined after 15 minutes incubation with 20 mM AAPH (left). Conditions otherwise as in the previous panels. (*** p
    Infectious Pseudoviral Particles, supplied by Syntaxin, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/infectious pseudoviral particles/product/Syntaxin
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    infectious pseudoviral particles - by Bioz Stars, 2020-08
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    85
    BIO-CAT 293tn human pseudoviral particle producer
    Effect of membrane-active compounds on HIV membrane lateral packing (left panels) and <t>pseudovirus</t> cell entry (right panels). ( a ) Left: Samples were incubated with M β CD (1 mM) for 15 minutes before collecting GP images from Laurdan-labeled individual GUVs. The group of vesicles that displayed better fitting to two-component distributions is plotted using two boxes, the upper one corresponding to the more ordered domains and the lower one to the more disordered regions. Right: HIV-1 pseudoviruses were pre-attached to lysine-coated plates 57 and treated with increasing concentrations of M β CD. After washing, reporter TZM-bl cells were layered on top, and entry (gene transduction) inferred from the number of total cells expressing GFP, as described previously 39 . Values represent means ± SD of three independent assays. ( b ) Samples were treated with CpreTM peptide. Average GP values were determined for GUVs treated for at least 15 minutes with 1 μM peptide (left). The population of GUVs displaying membrane diaphragms were sorted out and measured separately. ( c ) Samples incubated with AAPH. GP values were determined after 15 minutes incubation with 20 mM AAPH (left). Conditions otherwise as in the previous panels. (*** p
    293tn Human Pseudoviral Particle Producer, supplied by BIO-CAT, used in various techniques. Bioz Stars score: 85/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    BIO-CAT 293tn pseudoviral particle producer cells
    Effect of membrane-active compounds on HIV membrane lateral packing (left panels) and <t>pseudovirus</t> cell entry (right panels). ( a ) Left: Samples were incubated with M β CD (1 mM) for 15 minutes before collecting GP images from Laurdan-labeled individual GUVs. The group of vesicles that displayed better fitting to two-component distributions is plotted using two boxes, the upper one corresponding to the more ordered domains and the lower one to the more disordered regions. Right: HIV-1 pseudoviruses were pre-attached to lysine-coated plates 57 and treated with increasing concentrations of M β CD. After washing, reporter TZM-bl cells were layered on top, and entry (gene transduction) inferred from the number of total cells expressing GFP, as described previously 39 . Values represent means ± SD of three independent assays. ( b ) Samples were treated with CpreTM peptide. Average GP values were determined for GUVs treated for at least 15 minutes with 1 μM peptide (left). The population of GUVs displaying membrane diaphragms were sorted out and measured separately. ( c ) Samples incubated with AAPH. GP values were determined after 15 minutes incubation with 20 mM AAPH (left). Conditions otherwise as in the previous panels. (*** p
    293tn Pseudoviral Particle Producer Cells, supplied by BIO-CAT, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/293tn pseudoviral particle producer cells/product/BIO-CAT
    Average 85 stars, based on 10 article reviews
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    88
    BIO-CAT variant 293tn pseudoviral particle producer cells
    Effect of membrane-active compounds on HIV membrane lateral packing (left panels) and <t>pseudovirus</t> cell entry (right panels). ( a ) Left: Samples were incubated with M β CD (1 mM) for 15 minutes before collecting GP images from Laurdan-labeled individual GUVs. The group of vesicles that displayed better fitting to two-component distributions is plotted using two boxes, the upper one corresponding to the more ordered domains and the lower one to the more disordered regions. Right: HIV-1 pseudoviruses were pre-attached to lysine-coated plates 57 and treated with increasing concentrations of M β CD. After washing, reporter TZM-bl cells were layered on top, and entry (gene transduction) inferred from the number of total cells expressing GFP, as described previously 39 . Values represent means ± SD of three independent assays. ( b ) Samples were treated with CpreTM peptide. Average GP values were determined for GUVs treated for at least 15 minutes with 1 μM peptide (left). The population of GUVs displaying membrane diaphragms were sorted out and measured separately. ( c ) Samples incubated with AAPH. GP values were determined after 15 minutes incubation with 20 mM AAPH (left). Conditions otherwise as in the previous panels. (*** p
    Variant 293tn Pseudoviral Particle Producer Cells, supplied by BIO-CAT, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Addgene inc tronolab
    Effect of membrane-active compounds on HIV membrane lateral packing (left panels) and <t>pseudovirus</t> cell entry (right panels). ( a ) Left: Samples were incubated with M β CD (1 mM) for 15 minutes before collecting GP images from Laurdan-labeled individual GUVs. The group of vesicles that displayed better fitting to two-component distributions is plotted using two boxes, the upper one corresponding to the more ordered domains and the lower one to the more disordered regions. Right: HIV-1 pseudoviruses were pre-attached to lysine-coated plates 57 and treated with increasing concentrations of M β CD. After washing, reporter TZM-bl cells were layered on top, and entry (gene transduction) inferred from the number of total cells expressing GFP, as described previously 39 . Values represent means ± SD of three independent assays. ( b ) Samples were treated with CpreTM peptide. Average GP values were determined for GUVs treated for at least 15 minutes with 1 μM peptide (left). The population of GUVs displaying membrane diaphragms were sorted out and measured separately. ( c ) Samples incubated with AAPH. GP values were determined after 15 minutes incubation with 20 mM AAPH (left). Conditions otherwise as in the previous panels. (*** p
    Tronolab, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effect of membrane-active compounds on HIV membrane lateral packing (left panels) and pseudovirus cell entry (right panels). ( a ) Left: Samples were incubated with M β CD (1 mM) for 15 minutes before collecting GP images from Laurdan-labeled individual GUVs. The group of vesicles that displayed better fitting to two-component distributions is plotted using two boxes, the upper one corresponding to the more ordered domains and the lower one to the more disordered regions. Right: HIV-1 pseudoviruses were pre-attached to lysine-coated plates 57 and treated with increasing concentrations of M β CD. After washing, reporter TZM-bl cells were layered on top, and entry (gene transduction) inferred from the number of total cells expressing GFP, as described previously 39 . Values represent means ± SD of three independent assays. ( b ) Samples were treated with CpreTM peptide. Average GP values were determined for GUVs treated for at least 15 minutes with 1 μM peptide (left). The population of GUVs displaying membrane diaphragms were sorted out and measured separately. ( c ) Samples incubated with AAPH. GP values were determined after 15 minutes incubation with 20 mM AAPH (left). Conditions otherwise as in the previous panels. (*** p

    Journal: Scientific Reports

    Article Title: Functional organization of the HIV lipid envelope

    doi: 10.1038/srep34190

    Figure Lengend Snippet: Effect of membrane-active compounds on HIV membrane lateral packing (left panels) and pseudovirus cell entry (right panels). ( a ) Left: Samples were incubated with M β CD (1 mM) for 15 minutes before collecting GP images from Laurdan-labeled individual GUVs. The group of vesicles that displayed better fitting to two-component distributions is plotted using two boxes, the upper one corresponding to the more ordered domains and the lower one to the more disordered regions. Right: HIV-1 pseudoviruses were pre-attached to lysine-coated plates 57 and treated with increasing concentrations of M β CD. After washing, reporter TZM-bl cells were layered on top, and entry (gene transduction) inferred from the number of total cells expressing GFP, as described previously 39 . Values represent means ± SD of three independent assays. ( b ) Samples were treated with CpreTM peptide. Average GP values were determined for GUVs treated for at least 15 minutes with 1 μM peptide (left). The population of GUVs displaying membrane diaphragms were sorted out and measured separately. ( c ) Samples incubated with AAPH. GP values were determined after 15 minutes incubation with 20 mM AAPH (left). Conditions otherwise as in the previous panels. (*** p

    Article Snippet: Two days after transfection, the pseudovirus particles were harvested, passed through 0.45 μm pore sterile filters (Millex® HV, Millipore NV, Brussels, Belgium) and finally concentrated by ultracentrifugation in a sucrose gradient.

    Techniques: Incubation, Labeling, Transduction, Expressing