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  • 94
    Alomone Labs pctx1
    Pctx1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pctx1/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pctx1 - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    86
    Biosynth Carbosynth psalmotoxin 1 pctx1
    The effects of ASIC channel blockers on ASIC currents in freshly isolated anterior pituitary cells. A . Representative ASIC currents elicited by pH 5.5 with various concentrations of amiloride. Holding potential was −70 mV. The cells were held at pH 7.4 before and in between applications of pH 5.5. Amiloride was applied during acid application. B . Summary data showing the dose-dependent block of ASIC currents by amiloride in anterior pituitary cells (n = 7). Currents were normalized to the control condition (without amiloride). The IC 50 of amiloride block was 6.3±1.0 µM (n = 7). C. Representative ASIC currents in WT and ASIC2 -/- cells elicited by pH 5.5 with 100 nM <t>PcTx1.</t> Cells were pre-treated with PcTx1 in pH 7.4 for 1 min and then were perfused with pH 5.5 containing 100 nM PcTx1. Holding potential was −70 mV. D. Summary data showing the block of ASIC currents by 100 nM PcTx1 in anterior pituitary cells from WT (n = 7) and ASIC2 -/- mice (n = 6). * P
    Psalmotoxin 1 Pctx1, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psalmotoxin 1 pctx1/product/Biosynth Carbosynth
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    psalmotoxin 1 pctx1 - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

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    The effects of ASIC channel blockers on ASIC currents in freshly isolated anterior pituitary cells. A . Representative ASIC currents elicited by pH 5.5 with various concentrations of amiloride. Holding potential was −70 mV. The cells were held at pH 7.4 before and in between applications of pH 5.5. Amiloride was applied during acid application. B . Summary data showing the dose-dependent block of ASIC currents by amiloride in anterior pituitary cells (n = 7). Currents were normalized to the control condition (without amiloride). The IC 50 of amiloride block was 6.3±1.0 µM (n = 7). C. Representative ASIC currents in WT and ASIC2 -/- cells elicited by pH 5.5 with 100 nM PcTx1. Cells were pre-treated with PcTx1 in pH 7.4 for 1 min and then were perfused with pH 5.5 containing 100 nM PcTx1. Holding potential was −70 mV. D. Summary data showing the block of ASIC currents by 100 nM PcTx1 in anterior pituitary cells from WT (n = 7) and ASIC2 -/- mice (n = 6). * P

    Journal: PLoS ONE

    Article Title: Expression and Activity of Acid-Sensing Ion Channels in the Mouse Anterior Pituitary

    doi: 10.1371/journal.pone.0115310

    Figure Lengend Snippet: The effects of ASIC channel blockers on ASIC currents in freshly isolated anterior pituitary cells. A . Representative ASIC currents elicited by pH 5.5 with various concentrations of amiloride. Holding potential was −70 mV. The cells were held at pH 7.4 before and in between applications of pH 5.5. Amiloride was applied during acid application. B . Summary data showing the dose-dependent block of ASIC currents by amiloride in anterior pituitary cells (n = 7). Currents were normalized to the control condition (without amiloride). The IC 50 of amiloride block was 6.3±1.0 µM (n = 7). C. Representative ASIC currents in WT and ASIC2 -/- cells elicited by pH 5.5 with 100 nM PcTx1. Cells were pre-treated with PcTx1 in pH 7.4 for 1 min and then were perfused with pH 5.5 containing 100 nM PcTx1. Holding potential was −70 mV. D. Summary data showing the block of ASIC currents by 100 nM PcTx1 in anterior pituitary cells from WT (n = 7) and ASIC2 -/- mice (n = 6). * P

    Article Snippet: Psalmotoxin-1 (PcTX1) was ordered from Peptides International.

    Techniques: Isolation, Blocking Assay, Mouse Assay