Journal: Scientific Reports
Article Title: Landscape of heart proteome changes in a diet-induced obesity model
Figure Lengend Snippet: Biological functions of the differentially expressed proteins in the myocardium of obese rats identified by proteomics ( A ) Protein-protein interaction network. The interaction network analyses were built using the STRING online software with a medium confidence level (0.4). The circles represent proteins, while the straight lines represent the interactions between different proteins. The line thickness indicates the strength of evidence, with thicker connections indicating higher confidence in the protein-protein interaction. Green circles represent the proteins involved in metabolic processes; shaded area delimits the proteins involved in lipid metabolic processes. ( B ) Gene ontology (GO) enrichment analysis. The analysis was performed using the PANTHER tool ( http://www.pantherdb.org ), providing the significantly enriched GO terms Molecular Function, Biological Process, and Cellular Component. On the left panel, the horizontal axis indicates the significance (−log10 p -value) of the functional association, which is dependent on the number of proteins in the class. On the right panel, changes are displayed as the number of proteins with increased or decreased levels (horizontal axis). ( C ) Violin plot of the fold changes for up- and down-regulated proteins in the WD group compared to their controls. The fold changes of up- and down-regulated proteins were further separated into the metabolic processes associated with fatty acid, glucose, amino acid, tricarboxylic acid (TCA) cycle, and oxidative phosphorylation (Oxi. Phosp.). The circles inside the plots represent the changed proteins. The detailed description of protein names is shown in Supplementary Tables S3 and S4 .
Article Snippet: The MS raw data files were loaded into Progenesis QI for Proteomics v.4.0 (Nonlinear Dynamics, Waters, Newcastle upon Tyne, UK) to perform the quantitative analysis.
Techniques: Software, Functional Assay