Journal: Frontiers in Plant Science
Article Title: Identification of a Novel Small Cysteine-Rich Protein in the Fraction from the Biocontrol Fusarium oxysporum Strain CS-20 that Mitigates Fusarium Wilt Symptoms and Triggers Defense Responses in Tomato
Figure Lengend Snippet: Comparison of anti-wilt activity of the fractions obtained by exclusion chromatography of high-molecular weight metabolites from F. oxysporum strain CS-20. Seedlings were inoculated by immersion in a suspension with a final concentration of 10 6 spores/ml, containing spores of two pathogenic strains F37 and Fot3 (1:1). Disease symptoms were examined at 15 (A) , and 21 (B) days after inoculation of tomato seedlings, which roots were pre-exposed to intact (dark gray columns) or proteinase K-treated (light gray columns) fractions eluted as peaks III, IV, and V. Inoculated seedlings non-exposed to the fractions are referred to as infected control (IfCtrl, black columns). Histograms represent values of average disease index from two independent experiments of each treatment done in triplicate. Bars represent SD.
Article Snippet: Protein Digestion Assay Samples of the protein-containing fractions isolated by size-exclusion chromatography were treated with proteinase K (Promega, USA) following a protocol recommended by the manufacturer for native protein cleavage .
Techniques: Activity Assay, Chromatography, Molecular Weight, Concentration Assay, Infection