proteinase k Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Qiagen protease k
    Protease K, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 402 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease k/product/Qiagen
    Average 99 stars, based on 402 article reviews
    Price from $9.99 to $1999.99
    protease k - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher protease k
    Protease K, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 300 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease k/product/Thermo Fisher
    Average 99 stars, based on 300 article reviews
    Price from $9.99 to $1999.99
    protease k - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    90
    TaKaRa protease k
    Protease K, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease k/product/TaKaRa
    Average 90 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    protease k - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    89
    Abcam protease k
    Protease K, supplied by Abcam, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease k/product/Abcam
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protease k - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    92
    Boster Bio protease k
    Protease K, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease k/product/Boster Bio
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    protease k - by Bioz Stars, 2020-01
    92/100 stars
      Buy from Supplier

    80
    Thermo Fisher protease k solution
    Protease K Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease k solution/product/Thermo Fisher
    Average 80 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    protease k solution - by Bioz Stars, 2020-01
    80/100 stars
      Buy from Supplier

    89
    Worthington Biochemical protease k
    Protease K, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease k/product/Worthington Biochemical
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protease k - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    99
    Thermo Fisher proteinase k
    Proteinase K, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16083 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k/product/Thermo Fisher
    Average 99 stars, based on 16083 article reviews
    Price from $9.99 to $1999.99
    proteinase k - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    97
    Abcam proteinase k
    Proteinase K, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k/product/Abcam
    Average 97 stars, based on 261 article reviews
    Price from $9.99 to $1999.99
    proteinase k - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    95
    Qiagen puregene proteinase k
    Puregene Proteinase K, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/puregene proteinase k/product/Qiagen
    Average 95 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    puregene proteinase k - by Bioz Stars, 2020-01
    95/100 stars
      Buy from Supplier

    93
    Novus Biologicals proteinase k
    Proteinase K, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k/product/Novus Biologicals
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    proteinase k - by Bioz Stars, 2020-01
    93/100 stars
      Buy from Supplier

    86
    Millipore proteinase k p2308
    Proteinase K P2308, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k p2308/product/Millipore
    Average 86 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    proteinase k p2308 - by Bioz Stars, 2020-01
    86/100 stars
      Buy from Supplier

    88
    Roche proteinase k phosphostop
    Proteinase K Phosphostop, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k phosphostop/product/Roche
    Average 88 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    proteinase k phosphostop - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    78
    BioTechniques proteinase k biotechniques
    Proteinase K Biotechniques, supplied by BioTechniques, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k biotechniques/product/BioTechniques
    Average 78 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    proteinase k biotechniques - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    99
    Thermo Fisher proteinase k powder
    Proteinase K Powder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k powder/product/Thermo Fisher
    Average 99 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    proteinase k powder - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    88
    Kurabo industries proteinase k buffer
    Proteinase K Buffer, supplied by Kurabo industries, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k buffer/product/Kurabo industries
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    proteinase k buffer - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    88
    Becton Dickinson proteinase k buffer
    Proteinase K Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k buffer/product/Becton Dickinson
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    proteinase k buffer - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    88
    Merck & Co proteinase k buffer
    Proteinase K Buffer, supplied by Merck & Co, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k buffer/product/Merck & Co
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    proteinase k buffer - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    79
    Promega nacl proteinase k
    Nacl Proteinase K, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nacl proteinase k/product/Promega
    Average 79 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nacl proteinase k - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    99
    Millipore protease k
    Localization of ErbB2 in mitochondria. (A) Cytosolic, nuclear, mitochondrial, and plasma membrane proteins were isolated and subjected to SDS-PAGE followed by probing with indicated antibodies. Two exogenous ErbB2 overexpressing breast cancer cell lines MCF7/ErbB2 and MDA-MB-231 (left), and two natural ErbB2-positive breast cancer cell lines SKBR3 and BT474 (right) were used for Western Blotting. VDAC1 and prohibitin were mitochondrial markers; Integrin β1 and IGF1Rα were plasma membrane markers; α-Tubulin and ERK were cytoplasmic markers; KDEL was an ER marker; EEA1 was an early endosomes marker; Golgi complex was a marker for the detection of Golgi; LAMP2 was an lysosome marker and Lamin B1 was a nucleus marker. (B) MCF7 cells, mouse heart and liver tissues, and ErbB2 positive and negative breast cancer patient samples were analyzed by Western blotting. (C) Co-localization of ErbB2 and mitochondria. Mitochondria were stained with Mitotracker-Green in ErbB2 transfected MCF7 cells. The cells were fixed and incubated with antibodies against ErbB2 (mouse), followed by incubation of monoclonal mouse Anti–Cy3 antibody (red). Images were analyzed with Nikon NIS-Elements AR software. Green: mitochondria; Red: ErbB2; Yellow: Co-localization of ErbB2 and mitochondria. The lower panel contains images with a higher magnification. Scale bars: 20 μm. (D) Localization of ErbB2 inside mitochondria. Intact mitochondria of SKBR3 cells were isolated and treated with <t>Protease</t> K at 0.5 mg/ml for 15 min at room temperature. 1% Triton was added into the reaction and 2 mM PMSF was added to stop the reaction. Each reaction was started with an equal amount/volume of mitochondria lysate. Mitochondrial lysate then was loaded onto 8% SDS PAGE followed by blotting with anti-ErbB2, anti-BCL-2 and anti-Prohibitin antibodies.
    Protease K, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease k/product/Millipore
    Average 99 stars, based on 418 article reviews
    Price from $9.99 to $1999.99
    protease k - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    89
    Applichem protease k
    Localization of ErbB2 in mitochondria. (A) Cytosolic, nuclear, mitochondrial, and plasma membrane proteins were isolated and subjected to SDS-PAGE followed by probing with indicated antibodies. Two exogenous ErbB2 overexpressing breast cancer cell lines MCF7/ErbB2 and MDA-MB-231 (left), and two natural ErbB2-positive breast cancer cell lines SKBR3 and BT474 (right) were used for Western Blotting. VDAC1 and prohibitin were mitochondrial markers; Integrin β1 and IGF1Rα were plasma membrane markers; α-Tubulin and ERK were cytoplasmic markers; KDEL was an ER marker; EEA1 was an early endosomes marker; Golgi complex was a marker for the detection of Golgi; LAMP2 was an lysosome marker and Lamin B1 was a nucleus marker. (B) MCF7 cells, mouse heart and liver tissues, and ErbB2 positive and negative breast cancer patient samples were analyzed by Western blotting. (C) Co-localization of ErbB2 and mitochondria. Mitochondria were stained with Mitotracker-Green in ErbB2 transfected MCF7 cells. The cells were fixed and incubated with antibodies against ErbB2 (mouse), followed by incubation of monoclonal mouse Anti–Cy3 antibody (red). Images were analyzed with Nikon NIS-Elements AR software. Green: mitochondria; Red: ErbB2; Yellow: Co-localization of ErbB2 and mitochondria. The lower panel contains images with a higher magnification. Scale bars: 20 μm. (D) Localization of ErbB2 inside mitochondria. Intact mitochondria of SKBR3 cells were isolated and treated with <t>Protease</t> K at 0.5 mg/ml for 15 min at room temperature. 1% Triton was added into the reaction and 2 mM PMSF was added to stop the reaction. Each reaction was started with an equal amount/volume of mitochondria lysate. Mitochondrial lysate then was loaded onto 8% SDS PAGE followed by blotting with anti-ErbB2, anti-BCL-2 and anti-Prohibitin antibodies.
    Protease K, supplied by Applichem, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease k/product/Applichem
    Average 89 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    protease k - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    93
    Amresco protease k
    Mtb WhiB4 exhibits non-specific DNA binding activities.  (A) 150 ng of either linear (L) or supercoiled (SC) plasmid DNA was incubated with oxidized apo-WhiB4 (250 ng [lanes 2, 6]; 300 ng [lanes 3, 7]; 350 ng [4, 8]). Lanes 1 and 5 represent DNA without WhiB4.  (B)  Binding of apo-WhiB4 (100, 150, 200, 250, 300 and 350 ng) with 1 kb λ-DNA ladder (λ) (150 ng).  (C) WhiB4 represses transcription .  In vitro  transcribed pGEM plasmid in the absence of apo-WhiB4 (lane 1), oxidized apo-WhiB4 in complex with pGEM (lane 2),  in vitro  transcription of pGEM in the presence of oxidized (lane 3) or reduced (lane 4) apo-WhiB4. The reactions in lane 3 and 4 were treated with 6% SDS and 4 mg ml -1  protease K to remove WhiB4 from the mixture before separation. C: pGEM plasmid DNA alone.  Cysteine residues regulate DNA binding of WhiB4 (D)  DTT-treatment abolished DNA binding of WhiB4. Oxidized apo-WhiB4 (350 ng) was treated with DTT (0, 200, 400, 800 mM) for 2 h followed by binding to the supercoiled plasmid DNA. C: represents DNA without WhiB4.  (E)  Purified wt apo-WhiB4 and Cys mutant variants were treated with thiol oxidant, diamide, and the gel-shift assay was performed as described earlier. DNA binding assays using  Mtb  HU (50–350 ng) and  Mtb  TcrX (150–350 ng) with  (F)  supercoiled DNA and  (G)  1 kb λ-DNA ladder.  (H)  350 ng of  Mtb  HU or (I)  Mtb  TcrX were treated with increasing concentrations of DTT (0, 200, 400, 800 mM) for 2 h followed by binding to the supercoiled plasmid DNA. The samples were analyzed on 1% agarose gel.
    Protease K, supplied by Amresco, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease k/product/Amresco
    Average 93 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    protease k - by Bioz Stars, 2020-01
    93/100 stars
      Buy from Supplier

    89
    Boehringer Mannheim protease k
    <t>Protease</t> K resistance of Sup35pN filamentous aggregates ( a ) and of amorphous aggregates ( b ) as determined by SDS/PAGE. The protease K concentration in each reaction is indicated.
    Protease K, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 89/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease k/product/Boehringer Mannheim
    Average 89 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    protease k - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    97
    New England Biolabs proteinase k
    <t>Protease</t> K resistance of Sup35pN filamentous aggregates ( a ) and of amorphous aggregates ( b ) as determined by SDS/PAGE. The protease K concentration in each reaction is indicated.
    Proteinase K, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 3073 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k/product/New England Biolabs
    Average 97 stars, based on 3073 article reviews
    Price from $9.99 to $1999.99
    proteinase k - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    Image Search Results


    Localization of ErbB2 in mitochondria. (A) Cytosolic, nuclear, mitochondrial, and plasma membrane proteins were isolated and subjected to SDS-PAGE followed by probing with indicated antibodies. Two exogenous ErbB2 overexpressing breast cancer cell lines MCF7/ErbB2 and MDA-MB-231 (left), and two natural ErbB2-positive breast cancer cell lines SKBR3 and BT474 (right) were used for Western Blotting. VDAC1 and prohibitin were mitochondrial markers; Integrin β1 and IGF1Rα were plasma membrane markers; α-Tubulin and ERK were cytoplasmic markers; KDEL was an ER marker; EEA1 was an early endosomes marker; Golgi complex was a marker for the detection of Golgi; LAMP2 was an lysosome marker and Lamin B1 was a nucleus marker. (B) MCF7 cells, mouse heart and liver tissues, and ErbB2 positive and negative breast cancer patient samples were analyzed by Western blotting. (C) Co-localization of ErbB2 and mitochondria. Mitochondria were stained with Mitotracker-Green in ErbB2 transfected MCF7 cells. The cells were fixed and incubated with antibodies against ErbB2 (mouse), followed by incubation of monoclonal mouse Anti–Cy3 antibody (red). Images were analyzed with Nikon NIS-Elements AR software. Green: mitochondria; Red: ErbB2; Yellow: Co-localization of ErbB2 and mitochondria. The lower panel contains images with a higher magnification. Scale bars: 20 μm. (D) Localization of ErbB2 inside mitochondria. Intact mitochondria of SKBR3 cells were isolated and treated with Protease K at 0.5 mg/ml for 15 min at room temperature. 1% Triton was added into the reaction and 2 mM PMSF was added to stop the reaction. Each reaction was started with an equal amount/volume of mitochondria lysate. Mitochondrial lysate then was loaded onto 8% SDS PAGE followed by blotting with anti-ErbB2, anti-BCL-2 and anti-Prohibitin antibodies.

    Journal: Nature communications

    Article Title: Receptor Tyrosine Kinase ErbB2 Translocates into Mitochondria and Regulates Cellular Metabolism

    doi: 10.1038/ncomms2236

    Figure Lengend Snippet: Localization of ErbB2 in mitochondria. (A) Cytosolic, nuclear, mitochondrial, and plasma membrane proteins were isolated and subjected to SDS-PAGE followed by probing with indicated antibodies. Two exogenous ErbB2 overexpressing breast cancer cell lines MCF7/ErbB2 and MDA-MB-231 (left), and two natural ErbB2-positive breast cancer cell lines SKBR3 and BT474 (right) were used for Western Blotting. VDAC1 and prohibitin were mitochondrial markers; Integrin β1 and IGF1Rα were plasma membrane markers; α-Tubulin and ERK were cytoplasmic markers; KDEL was an ER marker; EEA1 was an early endosomes marker; Golgi complex was a marker for the detection of Golgi; LAMP2 was an lysosome marker and Lamin B1 was a nucleus marker. (B) MCF7 cells, mouse heart and liver tissues, and ErbB2 positive and negative breast cancer patient samples were analyzed by Western blotting. (C) Co-localization of ErbB2 and mitochondria. Mitochondria were stained with Mitotracker-Green in ErbB2 transfected MCF7 cells. The cells were fixed and incubated with antibodies against ErbB2 (mouse), followed by incubation of monoclonal mouse Anti–Cy3 antibody (red). Images were analyzed with Nikon NIS-Elements AR software. Green: mitochondria; Red: ErbB2; Yellow: Co-localization of ErbB2 and mitochondria. The lower panel contains images with a higher magnification. Scale bars: 20 μm. (D) Localization of ErbB2 inside mitochondria. Intact mitochondria of SKBR3 cells were isolated and treated with Protease K at 0.5 mg/ml for 15 min at room temperature. 1% Triton was added into the reaction and 2 mM PMSF was added to stop the reaction. Each reaction was started with an equal amount/volume of mitochondria lysate. Mitochondrial lysate then was loaded onto 8% SDS PAGE followed by blotting with anti-ErbB2, anti-BCL-2 and anti-Prohibitin antibodies.

    Article Snippet: Protease K was purchased from Sigma; the immunoprecipitation assay was conducted using Pierce Co-immunoprecipitation Kit (Cat: 26149) from Thermo Scientific.

    Techniques: Isolation, SDS Page, Multiple Displacement Amplification, Western Blot, Marker, Staining, Transfection, Incubation, Software

    Mtb WhiB4 exhibits non-specific DNA binding activities.  (A) 150 ng of either linear (L) or supercoiled (SC) plasmid DNA was incubated with oxidized apo-WhiB4 (250 ng [lanes 2, 6]; 300 ng [lanes 3, 7]; 350 ng [4, 8]). Lanes 1 and 5 represent DNA without WhiB4.  (B)  Binding of apo-WhiB4 (100, 150, 200, 250, 300 and 350 ng) with 1 kb λ-DNA ladder (λ) (150 ng).  (C) WhiB4 represses transcription .  In vitro  transcribed pGEM plasmid in the absence of apo-WhiB4 (lane 1), oxidized apo-WhiB4 in complex with pGEM (lane 2),  in vitro  transcription of pGEM in the presence of oxidized (lane 3) or reduced (lane 4) apo-WhiB4. The reactions in lane 3 and 4 were treated with 6% SDS and 4 mg ml -1  protease K to remove WhiB4 from the mixture before separation. C: pGEM plasmid DNA alone.  Cysteine residues regulate DNA binding of WhiB4 (D)  DTT-treatment abolished DNA binding of WhiB4. Oxidized apo-WhiB4 (350 ng) was treated with DTT (0, 200, 400, 800 mM) for 2 h followed by binding to the supercoiled plasmid DNA. C: represents DNA without WhiB4.  (E)  Purified wt apo-WhiB4 and Cys mutant variants were treated with thiol oxidant, diamide, and the gel-shift assay was performed as described earlier. DNA binding assays using  Mtb  HU (50–350 ng) and  Mtb  TcrX (150–350 ng) with  (F)  supercoiled DNA and  (G)  1 kb λ-DNA ladder.  (H)  350 ng of  Mtb  HU or (I)  Mtb  TcrX were treated with increasing concentrations of DTT (0, 200, 400, 800 mM) for 2 h followed by binding to the supercoiled plasmid DNA. The samples were analyzed on 1% agarose gel.

    Journal: Redox Biology

    Article Title: Redox-dependent condensation of the mycobacterial nucleoid by WhiB4

    doi: 10.1016/j.redox.2018.08.006

    Figure Lengend Snippet: Mtb WhiB4 exhibits non-specific DNA binding activities. (A) 150 ng of either linear (L) or supercoiled (SC) plasmid DNA was incubated with oxidized apo-WhiB4 (250 ng [lanes 2, 6]; 300 ng [lanes 3, 7]; 350 ng [4, 8]). Lanes 1 and 5 represent DNA without WhiB4. (B) Binding of apo-WhiB4 (100, 150, 200, 250, 300 and 350 ng) with 1 kb λ-DNA ladder (λ) (150 ng). (C) WhiB4 represses transcription . In vitro transcribed pGEM plasmid in the absence of apo-WhiB4 (lane 1), oxidized apo-WhiB4 in complex with pGEM (lane 2), in vitro transcription of pGEM in the presence of oxidized (lane 3) or reduced (lane 4) apo-WhiB4. The reactions in lane 3 and 4 were treated with 6% SDS and 4 mg ml -1 protease K to remove WhiB4 from the mixture before separation. C: pGEM plasmid DNA alone. Cysteine residues regulate DNA binding of WhiB4 (D) DTT-treatment abolished DNA binding of WhiB4. Oxidized apo-WhiB4 (350 ng) was treated with DTT (0, 200, 400, 800 mM) for 2 h followed by binding to the supercoiled plasmid DNA. C: represents DNA without WhiB4. (E) Purified wt apo-WhiB4 and Cys mutant variants were treated with thiol oxidant, diamide, and the gel-shift assay was performed as described earlier. DNA binding assays using Mtb HU (50–350 ng) and Mtb TcrX (150–350 ng) with (F) supercoiled DNA and (G) 1 kb λ-DNA ladder. (H) 350 ng of Mtb HU or (I) Mtb TcrX were treated with increasing concentrations of DTT (0, 200, 400, 800 mM) for 2 h followed by binding to the supercoiled plasmid DNA. The samples were analyzed on 1% agarose gel.

    Article Snippet: Samples were treated with 6% SDS (Amresco Inc.) and 4 mg ml-1 proteinase K (Amresco Inc.) for 30 min at 37o C and the reaction products were analyzed on a 1% agarose gel.

    Techniques: Binding Assay, Plasmid Preparation, Incubation, In Vitro, Purification, Mutagenesis, Electrophoretic Mobility Shift Assay, Agarose Gel Electrophoresis

    Protease K resistance of Sup35pN filamentous aggregates ( a ) and of amorphous aggregates ( b ) as determined by SDS/PAGE. The protease K concentration in each reaction is indicated.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Prion-inducing domain 2-114 of yeast Sup35 protein transforms in vitro into amyloid-like filaments

    doi:

    Figure Lengend Snippet: Protease K resistance of Sup35pN filamentous aggregates ( a ) and of amorphous aggregates ( b ) as determined by SDS/PAGE. The protease K concentration in each reaction is indicated.

    Article Snippet: Two microliters of protease K (Boehringer Mannheim no. 1413 783) solution in TE buffer at concentrations from 0.5 to 4.0 μg/ml were added and the mixture was incubated at 37°C for 80 min.

    Techniques: SDS Page, Concentration Assay