Journal: Frontiers in Immunology

Article Title: Thrombospondin-1 mitigates osteoarthritis progression by inhibiting mechanical stress-induced chondrocyte ferroptosis via the integrin/YAP pathway

doi: 10.3389/fimmu.2025.1577234

Figure Lengend Snippet: THBS1 inhibits chondrocyte ferroptosis through the integrinαVβ1/YAP pathway. THBS1 knockout chondrocytes and normal chondrocytes were subjected to 1 MPa mechanical stress for 2 hours, with or without the addition of YAP1 inhibitor PROTAC YAP d1 (20μM) and rhTHBS1(100ng/ml). Immediately following stimulation, IF detection was performed. Additional analyses were conducted after chondrocytes were further incubated with or without YAP1 inhibitor PROTAC YAP d1 (20μM) and rhTHBS1(100ng/ml) for 24 hours post-stimulation. (A) Representative image of chondrocytes undergoing IF detection immediately after mechanical stress stimulation. YAP (red) and DAPI (blue) are also shown. Scale bar = 20 μm. (B) Quantification of YAP localization. In each experiment 150 to 200 cells were evaluated (n = 3). YAP localization in the nucleus (green) or cytoplasm (red) is shown. (C) Western blot (WB) analysis of YAP1. (D) Quantification of WB analysis (n=3 for each group). (E) Representative image of chondrocytes undergoing IF detection immediately after mechanical stress stimulation. YAP (red) and DAPI (blue) are also shown. Scale bar = 20 μm. (F) Quantification of YAP localization. In each experiment 150 to 200 cells were evaluated (n = 3). (G) Western blot (WB) analysis of YAP1. (H) Quantification of WB analysis (n=3 for each group). (I) Mitochondrial membrane potential was detected by JC-1 assay. Scale bar = 10 μm. (J) The relative IOD ratio of red fluorescence to green fluorescence was used for quantitative analysis (n=3 for each group). (K) Representative TEM images of chondrocytes of indicated groups. (n=3 for each group). Green arrows show the normal mitochondria. Red arrows show the shrunken mitochondria. Scale bars, 5.0 μm (low field), 500 nm (high field). (L) Representative fluorescence images of mitochondria in chondrocytes. Scale bar = 50 μm. (M) Quantitative analysis of fluorescence intensity (n=3 for each group). (N) The expression of GSH in chondrocytes of indicated groups was detected by ELISA (n=3 for each group). Data were presented as the mean ± SD. NS P>0.05 *P<0.05, **P<0.01.

Article Snippet: YAP1 inhibitor PROTAC YAP d1 was obtained from MCE (HY-168016).

Techniques: Knock-Out, Incubation, Western Blot, Membrane, Fluorescence, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Leukemia

Article Title: The non-canonical BAF chromatin remodeling complex is a novel target of spliceosome dysregulation in SF3B1 -mutated chronic lymphocytic leukemia

doi: 10.1038/s41375-024-02379-4

Figure Lengend Snippet: A ASE categories, including skipped exon (SE), alternative 3′ splice site usage (A3SS), alternative 5′ splice site usage (A5SS), mutually exclusive exon usage (MXE), and intron retention (RI). B Bar plot displaying numbers of all identified ASEs in the comparison of 18 SF3B1 MUT and 17 SF3B1 WT subset #2 cases across different ASE categories. 163,097 ASEs were detected and involved transcripts of 10,437 genes. C Volcano plot depicting all identified ASEs in the comparison of SF3B1 MUT and SF3B1 WT subset #2 cases. Red dots indicate ASEs that are considered significant (|ΔPSI | ≥ 20% and FDR ≤ 0.01; 80 ASEs). D Bar plot displaying numbers of significant ASEs in the comparison of SF3B1 MUT and SF3B1 WT subset #2 cases across different ASE categories. 80 ASEs were considered significant and involved transcripts of 62 genes. E Heatmap illustrating the individual PSI values for the 80 significant ASEs detected in the comparison of SF3B1 MUT and SF3B1 WT subset #2 cases. 33 ASEs occurred within the same gene at least twice. ASEs are displayed based on the ASE category. The bar plot to the right of the heatmap shows ΔPSI values. The gene affected by each ASE and the corresponding unique ASE ID are listed. Genes that encode ncBAF complex-interacting proteins are depicted in color. For multiple ASEs per gene, only the top ASE is colored, while the others are marked with black asterisks. Additional details of these ASEs are provided in Supplementary Table . F Model of the ncBAF chromatin remodeling complex and previously reported ncBAF complex interactors. Alternatively spliced transcripts identified in SF3B1 MUT subset #2 CLL that encode ncBAF complex-related proteins are depicted in color. G Scattered box plot showing the PSI value distribution, ΔPSI values, and P values (Wilcoxon rank-sum test) for significant ncBAF complex-related ASEs identified in the comparison of SF3B1 MUT and SF3B1 WT subset #2 cases. The specific ASEs for ZEB1 , BRD9 , PLSCR1 , TENT4B , CXXC1 , DCAF16 , UBP1 , DLST , and SERBP1 have the unique ASE IDs ZEB1_SE_27141, BRD9_SE_53886, PLSCR1_SE_92684, PAPD5_SE_94061, CXXC1_SE_102397, DCAF16_SE_4627, UBP1_SE_85897, DLST_A3SS_2409, and SERBP1_A3SS_1321, respectively, in Supplementary Table . The boxes represent the median and the interquartile range (IQR), while the whiskers extend to 1.5 times the IQR from the first and third quartiles. ASE: alternative splicing event; WT: wildtype; MUT: mutated; FDR: false discovery rate; PSI: percent spliced in; VAF: variant allele frequency.

Article Snippet: Cell lines and primary CLL cells were treated with BRD9 inhibitors/degraders, I-BRD9 (SML1534; Sigma-Aldrich), PROTAC BRD9 Degrader-1 (HY-103632; MedChemExpress, New Jersey, NJ, USA), and dBRD9 Hydrochloride (SML2911; Sigma-Aldrich) [ , ].

Techniques: Comparison, Alternative Splicing, Variant Assay

Journal: Leukemia

Article Title: The non-canonical BAF chromatin remodeling complex is a novel target of spliceosome dysregulation in SF3B1 -mutated chronic lymphocytic leukemia

doi: 10.1038/s41375-024-02379-4

Figure Lengend Snippet: Sashimi plots illustrating the identified ASEs and alternative splicing patterns in four genes that encode ncBAF complex-related proteins in SF3B1 WT versus SF3B1 MUT CLL; ZEB1 and BRD9 exhibit the top two ASEs in the SE category, while DLST and SERBP1 showcase the top two ASEs in the A3SS category. Sashimi plots for other ncBAF complex-related genes, PLSCR1 , TENT4B , CXXC1 , DCAF16 , and UBP1 , are presented in Supplementary Fig. . For each gene, the top two sashimi plots within the gray box illustrate the predicted splice variants in SF3B1 WT versus SF3B1 MUT CLL. The colored arc highlights the primary ASE, while lighter arcs represent additional ASEs if present. The gene map indicates the relative location and order of the detected exons in relation to the sequencing results. For each corresponding gene, the lower two sashimi plots show the coverage and splice junction count data from the aligned long-read RNA-seq data from an SF3B1 WT case (RS24) and an SF3B1 MUT case (RS55), both belonging to subset #2 CLL. The direction of the genes is arranged from left to right. WT: wildtype; MUT: mutated; PSI: percent spliced in.

Article Snippet: Cell lines and primary CLL cells were treated with BRD9 inhibitors/degraders, I-BRD9 (SML1534; Sigma-Aldrich), PROTAC BRD9 Degrader-1 (HY-103632; MedChemExpress, New Jersey, NJ, USA), and dBRD9 Hydrochloride (SML2911; Sigma-Aldrich) [ , ].

Techniques: Alternative Splicing, Sequencing, RNA Sequencing Assay

Journal: Leukemia

Article Title: The non-canonical BAF chromatin remodeling complex is a novel target of spliceosome dysregulation in SF3B1 -mutated chronic lymphocytic leukemia

doi: 10.1038/s41375-024-02379-4

Figure Lengend Snippet: A Scheme illustrating lentiviral constructs for SF3B1 WT and SF3B1 K700E overexpression under the control of a DOX-inducible promoter. B Bar plots depicting expression levels of endogenous and exogenous SF3B1 upon overexpression of SF3B1 WT and SF3B1 K700E in MEC1 and PCL12 cell lines. The bar plots display the mean values of triplicates with error bars representing the standard deviation. C Scheme illustrating the identified ASE in BRD9 with the regular and alternative splicing pattern. The blue and red boxes illustrate constant and alternative exons, respectively. D Increased expression of the alternative BRD9 transcript upon overexpression of SF3B1 K700E as compared to SF3B1 WT in MEC1 and PCL12 cell lines. The checkerboard scheme at the top of the panel illustrates the experimental conditions. The bar plots display the expression levels of BRD9 transcripts in general (transcripts containing exon 14), the regular BRD9 transcript (transcripts containing exon 14 followed by exon 16), and the alternative BRD9 transcript (transcripts containing exon 14 followed by exon 15) as determined by qPCR. Results are normalized on GAPDH levels and expressed as fold difference between DOX+ and DOX− conditions with corresponding P values (Student’s t -test). The bar plots display the mean values of experimental triplicates, with error bars representing the standard deviation. E Agarose gel electrophoresis images of PCR products spanning from exon 14 to 16 and allowing for exon 15 inclusion show a higher abundance of alternative BRD9 transcript in SF3B1 K700E DOX+. For a complete gel image see Supplementary Fig. . F Assessment of BRD9 splice variants in SF3B1 WT and SF3B1 MUT CLL, AML, and UVM cell lines. MEC1, PGA1, and HG3 are SF3B1 WT CLL cell lines, CII an SF3B1 K666E CLL cell line, HNT34 an SF3B1 K700E AML cell line, and MEL202 an SF3B1 R625G UVM cell line. The bar plots display the expression levels of BRD9 transcripts in general, the regular BRD9 transcript, and the alternative BRD9 transcript as determined by qPCR. Results for BRD9 transcripts in general are normalized on GAPDH levels, whereas regular and alternative BRD9 transcript levels are normalized on levels of BRD9 transcripts in general and expressed as fold difference between MEC1 and other cell lines with corresponding P values (Student’s t -test). The bar plots display the mean values of experimental triplicates, with error bars representing the standard deviation. G Agarose gel electrophoresis image of PCR products spanning from exon 14 to 16 and allowing for exon 15 inclusion shows a higher abundance of alternative BRD9 transcript in SF3B1 MUT cell lines. For a complete gel image see Supplementary Fig. . H Sashimi plots from the SF3B1 WT CLL cell line HG3 and the SF3B1 MUT AML and UVM cell lines, HNT34 and MEL202, respectively, illustrating the alternatively spliced exon 15 in BRD9 . The plots for HG3 are based on direct long-read RNA-seq, and HNT34 (SRR8616208) and MEL202 (SRR12354765) on short-read RNA-seq. I BRD9 splice isoform expression in SF3B1 WT and SF3B1 MUT CLL cell lines determined by isoform-specific antibodies. An antibody specific to the C-terminus of the regular BRD9 isoform detected a single band in both SF3B1 WT and SF3B1 MUT cell lines. Conversely, an antibody targeting a constant epitope within the central part of BRD9, detected two bands in the SF3B1 MUT cell line CII and one band in the SF3B1 WT cell lines MEC1 and PGA1, corresponding to the regular higher molecular weight BRD9 isoform and the alternative lower molecular weight BRD9 isoform. For complete blot images see Supplementary Fig. . WT: wildtype; MUT: mutated; DOX: doxycycline.

Article Snippet: Cell lines and primary CLL cells were treated with BRD9 inhibitors/degraders, I-BRD9 (SML1534; Sigma-Aldrich), PROTAC BRD9 Degrader-1 (HY-103632; MedChemExpress, New Jersey, NJ, USA), and dBRD9 Hydrochloride (SML2911; Sigma-Aldrich) [ , ].

Techniques: Construct, Over Expression, Control, Expressing, Standard Deviation, Alternative Splicing, Agarose Gel Electrophoresis, RNA Sequencing Assay, Molecular Weight

Journal: Leukemia

Article Title: The non-canonical BAF chromatin remodeling complex is a novel target of spliceosome dysregulation in SF3B1 -mutated chronic lymphocytic leukemia

doi: 10.1038/s41375-024-02379-4

Figure Lengend Snippet: A Scheme illustrating BRD9 splice variants and predicted protein isoforms according to NCBI RefSeq (Annotation release GCF_000001405.40-RS_2023_03). The zoom-in view highlights the predicted protein sequence stemming from exon 15 inclusion in BRD9 . Upon translation of exon 15, a stop codon emerges near the end of the exon, resulting in a shorter splice isoform with an alternative C-terminus. B Scheme depicting lentiviral constructs for overexpression of the regular and alternative BRD9 splice variants. The corresponding DNA and protein sequences are available in Supplementary Information Appendix 1. C Heatmap illustrating the detected ncBAF complex subunits upon co-immunoprecipitation and subsequent mass spectrometry analysis with the regular or alternative BRD9 isoforms stably overexpressed in the HEK293T cell line. The relative protein levels are based on the total number of identified peptide-spectrum matches (PSMs) for the corresponding protein from the mass spectrometry analysis. The ncBAF complex model to the right of the heatmap illustrates that the alternative BRD9 isoform precipitated a majority of the ncBAF complex subunits. ACTB was not detected among the co-immunoprecipitates. The control cells expressing FLAG-V5-tagged YFP were used. D Volcano plot displaying differential interaction analysis of proteins that selectively interacted more with the regular or alternative BRD9 isoforms (multiple Student’s t -tests with Benjamini–Hochberg multiple testing correction). The top 5 candidates are highlighted in red. E Venn diagram showing a comparison of the significant differentially interacting proteins with the regular or alternative BRD9 isoforms and BRD9-interacting proteins reported by Gaudio et al. . The intersecting proteins are SPEN, BRCA2, and CHD9. F Western blot analysis of V5-tag and BICRA co-immunoprecipitates in the HEK293T cell line with stable overexpression of the regular and alternative BRD9 isoforms. In the V5-tag-immunoprecipitations, both overexpressed BRD9 isoforms precipitated BICRA together with SMARCA4 and SMARCC1. Reciprocal BICRA co-immunoprecipitations precipitated both overexpressed BRD9 isoforms, SMARCA4 and SMARCC1. The control cells were transduced with an empty lentiviral vector. For complete blot images see Supplementary Fig. . G Bar plots showing V5-tag and BICRA co-immunoprecipitation efficiency with BRD9, BICRA, SMARCA4, and SMARCC1. Results are expressed as fold differences between the regular and alternative BRD9 isoforms with corresponding P values (Student’s t -test). The bar plots display the mean values from three repeated experiments, with error bars representing the standard deviation. For detailed quantification and calculation see Supplementary Fig. . PSM: peptide-spectrum matches; FDR: false discovery rate; IP: immunoprecipitation.

Article Snippet: Cell lines and primary CLL cells were treated with BRD9 inhibitors/degraders, I-BRD9 (SML1534; Sigma-Aldrich), PROTAC BRD9 Degrader-1 (HY-103632; MedChemExpress, New Jersey, NJ, USA), and dBRD9 Hydrochloride (SML2911; Sigma-Aldrich) [ , ].

Techniques: Sequencing, Construct, Over Expression, Immunoprecipitation, Mass Spectrometry, Stable Transfection, Control, Expressing, Comparison, Western Blot, Transduction, Plasmid Preparation, Standard Deviation

Journal: Leukemia

Article Title: The non-canonical BAF chromatin remodeling complex is a novel target of spliceosome dysregulation in SF3B1 -mutated chronic lymphocytic leukemia

doi: 10.1038/s41375-024-02379-4

Figure Lengend Snippet: A Volcano plot depicting differentially expressed genes between 18 SF3B1 MUT and 17 SF3B1 WT subset #2 cases with 22 downregulated and 55 upregulated genes ( | log 2 FC | ≥ 0.58 and FDR < 0.01). B Unsupervised clustermap illustrating the distinct clustering of SF3B1 MUT and SF3B1 WT subset #2 cases based on differential gene expression. The four neighboring genes, NOL9 , TAS1R1 , ZBTB48 , and KLHL21 , located on chromosome 1 are depicted in color. The Ward method and the Euclidean metric were employed. C Karyoplot of chromosome 1 displaying differentially expressed genes between SF3B1 MUT and SF3B1 WT subset #2 cases. Examination of chromosomal positions revealed four neighboring genes, NOL9 , TAS1R1 , ZBTB48 , and KLHL21 . D Venn diagram showing the overlap of genes with differential expression between SF3B1 MUT and SF3B1 WT cases in three independent datasets; subset #2 CLL, ICGC CLLE-ES [ , ], and TCGA CLL [ , , ]. For the differential gene expression analyses of the latter two datasets see Supplementary Fig. . ZBTB48 appeared upregulated in all three datasets, while TAS1R1 in subset #2 CLL and ICGC CLLE-ES. E Venn diagram showing the overlap of genes with higher expression levels in SF3B1 MUT subset #2 cases and increased chromatin accessibility in SF3B1 MUT CLL cases from Beekman et al. . The overlap comprises NOL9 , TAS1R1 , ZBTB48 , and KLHL21 . F Coverage tracks from ChIP-seq data sourced from the ENCODE database [ , ], based on the CML cell line K562, illustrating the binding of BRD9 and the ncBAF complex subunits SMARCA4 and SMARCC2 to the gene quartet region on chromosome 1. The promoters for these genes appear active based on the signal for H3K27ac, H3K4me3, H3K9ac, and H3K79me2, and transcriptionally active based on the H3K36me3 signal, whereas transcriptional repression is low based on the low signal for H3K27me3 and H3K9me3. G Gene essentiality map showing the relationship between efficacy and selectivity of genes in shinyDepMap [ , ]. SF3B1 appears as a non-selective dependency with high efficacy, while BRD9 as a selective dependency with high efficacy. BRD9 is in the dependency region of PIK3R1 , EZH2 , and FBXW7 . H Functional similarity clustering showing a chromatin remodeling dependency cluster connected to the BRD9 dependency signature in shinyDepMap. BRD9 clusters with SMARCD1 , a BAF complex subunit, subunits of the Mediator complex, p300/CBP subunits, and subunits of the SAGA complex. I BRD9 dependency ranking of the 1086 DepMap (22Q2) cell lines. For CLL cell lines, gene effect scores are given within the parenthesis. WT: wildtype; MUT: mutated; FDR: false discovery rate; FC: fold change.

Article Snippet: Cell lines and primary CLL cells were treated with BRD9 inhibitors/degraders, I-BRD9 (SML1534; Sigma-Aldrich), PROTAC BRD9 Degrader-1 (HY-103632; MedChemExpress, New Jersey, NJ, USA), and dBRD9 Hydrochloride (SML2911; Sigma-Aldrich) [ , ].

Techniques: Expressing, ChIP-sequencing, Binding Assay, Functional Assay

Journal: Leukemia

Article Title: The non-canonical BAF chromatin remodeling complex is a novel target of spliceosome dysregulation in SF3B1 -mutated chronic lymphocytic leukemia

doi: 10.1038/s41375-024-02379-4

Figure Lengend Snippet: A Dose-response analysis of I-BRD9, PROTAC BRD9 Degrader-1, and dBRD9 treatments in 3 SF3B1 WT cell lines, MEC1, PGA1, and HG3 (all CLL), and 3 SF3B1 MUT cell lines, CII (CLL), HNT34 (AML), and MEL202 (UVM). The cell lines were treated with drug concentrations ranging from 0.001 to 50 µM for 3 days, and cell viability was determined by CellTiter-Glo 2.0. Complete cell killing was exclusively observed with I-BRD9 treatment, allowing for the determination of corresponding IC 50 values for each cell line. The HNT34 cell line exhibited sensitivity to all three drugs, thereby allowing for the determination of IC 50 values for all conditions. Dose-response curves are shown with 95% confidence intervals, while individual dots display the mean values of triplicates, with error bars representing the standard deviation. B I-BRD9 sensitivity profile in 958 cancer cell lines from GDSC . C Assessment of proliferation in an SF3B1 WT cell line, PGA1, and SF3B1 MUT cell lines, CII and HNT34, upon treatment with 10 µM I-BRD9, 25 µM PROTAC BRD9 Degrader-1, or 25 µM dBRD9. Cell lines were treated for 3 days with a 5-hour exposure to BrdU at the end, and proliferation was quantified by flow cytometry, measured as BrdU+ cells. Vehicle (DMSO) was used as the negative control for drug treatment, while cells not exposed to BrdU served as the negative control to assess the specificity of the anti-BrdU antibody. The upper left quadrants (BrdU+ cells) in the density plots represent the percentages of proliferating cells. D Bar plot displaying differences in the percentages of proliferating cells compared to negative controls with corresponding P values (one-way ANOVA). The bar plot displays the mean values from two repeated experiments, with error bars representing the standard deviation. E Assessment of apoptosis in the same cell lines and the identical samples as in ( C ). Treatment with 5 µM Camptothecin and vehicle (DMSO) were used as the positive and negative controls, respectively. Apoptosis was evaluated by Annexin V/PI staining and subsequent flow cytometry analysis. The lower left (Annexin V-/PI- cells), upper left (Annexin V+/PI- cells), and upper right (Annexin V+/PI+ cells) quadrants in the density plots represent the percentages of viable, early apoptotic, and late apoptotic cells, respectively. F Stacked bar plot displaying differences in the percentages of viable, early apoptotic, and late apoptotic cells compared to negative controls with corresponding P values (one-way ANOVA). The bar plot displays the mean values from two repeated experiments, with error bars representing the standard deviation. G Bar plot showing cell viability differences as determined by CellTiter-Glo 2.0 in the same cell lines under the same experimental conditions as in panels ( C , E ) with corresponding P values (one-way ANOVA). The bar plot displays the mean values from two repeated experiments in triplicates, with error bars representing the standard deviation. WT: wildtype; MUT: mutated; IC 50 : half-maximal inhibitory concentration; RLU: relative luminescence unit.

Article Snippet: Cell lines and primary CLL cells were treated with BRD9 inhibitors/degraders, I-BRD9 (SML1534; Sigma-Aldrich), PROTAC BRD9 Degrader-1 (HY-103632; MedChemExpress, New Jersey, NJ, USA), and dBRD9 Hydrochloride (SML2911; Sigma-Aldrich) [ , ].

Techniques: Standard Deviation, Flow Cytometry, Negative Control, Staining, Concentration Assay

Journal: Leukemia

Article Title: The non-canonical BAF chromatin remodeling complex is a novel target of spliceosome dysregulation in SF3B1 -mutated chronic lymphocytic leukemia

doi: 10.1038/s41375-024-02379-4

Figure Lengend Snippet: A Dose-response analysis of I-BRD9 treatment in 3 SF3B1 WT (SKL48, SKL152, SKL53) and 3 SF3B1 MUT (SKL147, SKL47, SKL157) primary CLL cell samples. The cell lines were treated with drug concentrations ranging from 0.001 to 50 µM for 2 days, and cell viability was determined by CellTiter-Glo 2.0. Dose-response curves are shown with 95% confidence intervals, while individual dots display the mean values of triplicates, with error bars representing the standard deviation. B Stacked density plots showing apoptosis assessment in the same primary CLL cell samples as in ( A ). Treatment with 5 µM Camptothecin and vehicle (DMSO) were used as the positive and negative controls, respectively. Apoptosis was evaluated by Annexin V/PI staining and subsequent flow cytometry analysis. The lower left (Annexin V-/PI- cells), upper left (Annexin V+/PI- cells), and upper right (Annexin V+/PI+ cells) quadrants in the density plots represent the percentages of viable, early apoptotic, and late apoptotic cells, respectively. C Stacked bar plot displaying differences in the percentages of viable, early apoptotic, and late apoptotic cells compared to negative controls with corresponding P values (Student’s t -test). The bar plot displays the mean values, with error bars representing the standard deviation. WT: wildtype; MUT: mutated; IC 50 : half-maximal inhibitory concentration; RLU: relative luminescence unit.

Article Snippet: Cell lines and primary CLL cells were treated with BRD9 inhibitors/degraders, I-BRD9 (SML1534; Sigma-Aldrich), PROTAC BRD9 Degrader-1 (HY-103632; MedChemExpress, New Jersey, NJ, USA), and dBRD9 Hydrochloride (SML2911; Sigma-Aldrich) [ , ].

Techniques: Standard Deviation, Staining, Flow Cytometry, Concentration Assay

Journal: International Journal of Biological Sciences

Article Title: The SGK3/GSK3β/β-catenin signaling promotes breast cancer stemness and confers resistance to alpelisib therapy

doi: 10.7150/ijbs.104850

Figure Lengend Snippet: SGK3 was significantly upregulated in alpelisib-resistant cell lines. (A) RNA profiles showing the upregulation of SGK3 in alpelisib-resistant cell lines. (B) Kaplan-Meier curves depicting luminal breast cancer patients with high and low SGK3 scores. (C) The RNA expression levels of SGK3 and tumor stemness related markers in parental and alpelisib-resistant cells. (D) The proportion of CD24-/CD44+ breast stem cell population in parental and alpelisib-resistant cells. (E) Tumor spheres formation abilities of parental cells and alpelisib-resistant cells. (F) Western blot for CD44 and c-Myc protein levels. (G) GO enrichment analysis of differentiated expressed genes in luminal breast cancer patients with high and low SGK3 expression. (H) Western blot for total and phosphorylated SGK3, GSK3β, β-catenin and ESR1. (I) Western blot showing the protein levels of β-catenin in cytoplasm and nucleus. P: parental cell, R: resistant cells (J) Immunofluorescence staining for β-catenin localization. (K) The RNA expression levels of ESR1 and SGK3 after ESR1 knockdown. (L) Western blots of ESR1 and SGK3 after knockdown of ESR1 in alpelisib-resistant cells. Unpaired t test, *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Alpelisib (BYL719) (Selleck, S2814), inavolisib (MCE, HY-101562), tamoxifen (MCE, HY-13757A), PROTAC SGK3 degrader-1 (SGK3-IN) (AbMole, M10382) and VPS34 inhibitor 1 (VPS34-IN1) (MCE, HY-12795) were diluted in DMSO.

Techniques: RNA Expression, Western Blot, Expressing, Immunofluorescence, Staining, Knockdown

Journal: International Journal of Biological Sciences

Article Title: The SGK3/GSK3β/β-catenin signaling promotes breast cancer stemness and confers resistance to alpelisib therapy

doi: 10.7150/ijbs.104850

Figure Lengend Snippet: SGK3 promoted breast cancer stemness and conferred resistance to BYL719 by activating β-catenin. (A) Overexpression of SGK3 in MCF7 and T47D parental cells as shown by qPCR. (B) Dose-response curves for BYL719 in control and SGK3 overexpressed MCF7 and T47D cells. (C) Effect of SGK3 overexpression on colony formation. (D) The proportion of CD24-/CD44+ stem cell population in control and SGK3 overexpressed breast cancer cells. (E) Expression of CD44 and c-Myc in SGK3 overexpressed cells. (F) Effect of SGK3 overexpression on tumor sphere formation. (G) Western blot for total and phosphorylated GSK3β and β-catenin in SGK3 overexpressed cells. (H) Western blot showing the protein levels of β-catenin in cytoplasm and nucleus after SGK3 overexpression. (I) Immunofluorescence staining for β-catenin subcellular localization in control and SGK3 overexpressed MCF7 and T47D cells. Unpaired t test, ***p < 0.001.

Article Snippet: Alpelisib (BYL719) (Selleck, S2814), inavolisib (MCE, HY-101562), tamoxifen (MCE, HY-13757A), PROTAC SGK3 degrader-1 (SGK3-IN) (AbMole, M10382) and VPS34 inhibitor 1 (VPS34-IN1) (MCE, HY-12795) were diluted in DMSO.

Techniques: Over Expression, Control, Expressing, Western Blot, Immunofluorescence, Staining

Journal: International Journal of Biological Sciences

Article Title: The SGK3/GSK3β/β-catenin signaling promotes breast cancer stemness and confers resistance to alpelisib therapy

doi: 10.7150/ijbs.104850

Figure Lengend Snippet: Depletion of SGK3 restored the sensitivity to BYL719 and enhanced the anti-tumor activity of alpelisib. (A) SGK3 knockdown efficiency in alpelisib-resistant cells MCF7R and T47DR. (B) Dose-response curves of BYL719 in control and SGK3 depleted MCF7R and T47DR cells. (C) Effect of SGK3 knockdown on colony formation. (D) Effect of SGK3 knockdown on tumor sphere formation. (E) The proportion of CD24-/CD44+ stem cell population after SGK3 knockdown as shown by flow cytometry. (F) Expression of stemness markers in SGK3-depleted cells. (G) Western blot showing the total and phosphorylated GSK3β and β-catenin after SGK3 knockdown. (H) Western blot showing the protein levels of β-catenin in cytoplasm and nucleus after SGK3 knockdown. (I) Immunofluorescence staining illustrating the subcellular translocation of β-catenin upon SGK3 knockdown. (J) Xenografts generated by orthotopically injection of control and SGK3-depleted MCF7R and T47DR cells with or without BYL719 treatment. In (A-E): **p < 0.01, ***p < 0.001 by unpaired t test. In (H): *p < 0.05, ***p < 0.001 by one-way ANOVA test.

Article Snippet: Alpelisib (BYL719) (Selleck, S2814), inavolisib (MCE, HY-101562), tamoxifen (MCE, HY-13757A), PROTAC SGK3 degrader-1 (SGK3-IN) (AbMole, M10382) and VPS34 inhibitor 1 (VPS34-IN1) (MCE, HY-12795) were diluted in DMSO.

Techniques: Activity Assay, Knockdown, Control, Flow Cytometry, Expressing, Western Blot, Immunofluorescence, Staining, Translocation Assay, Generated, Injection

Journal: International Journal of Biological Sciences

Article Title: The SGK3/GSK3β/β-catenin signaling promotes breast cancer stemness and confers resistance to alpelisib therapy

doi: 10.7150/ijbs.104850

Figure Lengend Snippet: SGK3 inhibition blocked β-catenin activation and reduced the stemness phenotype. (A) Dose-response curves of SGK3 inhibitors namely VPS34-IN1 and SGK3-IN in alpelisib-resistant MCF7R and T47DR cells. (B) Western blot for total and phosphorylated SGK3, GSK3β and β-catenin after VPS34-IN1 and SGK3-IN treatment. (C) Effects of VPS34-IN1 and SGK3-IN on CD24-/CD44+ stem cell population in alpelisib-resistant cells. (D) Immunofluorescence images showing that the abundance and subcellular location of β-catenin in alpelisib-resistant cells treated with SGK3 inhibitors. (E) The growth curves of MCF7R and T47DR under the treatment of BYL719 and SGK3 inhibitors. Unpaired t test, **p < 0.01, ***p < 0.001.

Article Snippet: Alpelisib (BYL719) (Selleck, S2814), inavolisib (MCE, HY-101562), tamoxifen (MCE, HY-13757A), PROTAC SGK3 degrader-1 (SGK3-IN) (AbMole, M10382) and VPS34 inhibitor 1 (VPS34-IN1) (MCE, HY-12795) were diluted in DMSO.

Techniques: Inhibition, Activation Assay, Western Blot, Immunofluorescence