Journal: Experimental and Therapeutic Medicine

Article Title: Inhibition of focal adhesion kinase induces apoptosis in bladder cancer cells via Src and the phosphatidylinositol 3-kinase/Akt pathway

doi: 10.3892/etm.2015.2745

Figure Lengend Snippet: Phosphatidylinositol 3-kinase/Akt acts downstream of FAK signaling to regulate apoptosis in T24 bladder cancer cells. T24 bladder cancer cells were treated with LY294002. (A and B) The expression of Akt, pAkt, caspase-3 and c-caspase-3 was examined using western blotting. (C and D) Cell apoptosis was examined using (C) deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay and (D) Annexin V/propidium iodide. (E and F) The expression of FAK and pFAK was examined using western blotting. Scale bar, 200 µm. FAK, focal adhesion kinase; pFAK, phosphorylated FAK; c-caspase-3, cleaved caspase-3; TGFβ, transforming growth factor-β.

Article Snippet: The treated cells were stained with FITC, Annexin V and propidium iodide (PI), and the stained cells were analyzed using a FACSort™ flow cytometer (Becton-Dickinson, Franklin Lakes, NJ, USA) and evaluated with the CellQuest™ software system (BD Biosciences).

Techniques: Expressing, Western Blot, End Labeling

Journal: Experimental and Therapeutic Medicine

Article Title: Inhibition of focal adhesion kinase induces apoptosis in bladder cancer cells via Src and the phosphatidylinositol 3-kinase/Akt pathway

doi: 10.3892/etm.2015.2745

Figure Lengend Snippet: Knockdown of FAK induces apoptosis in T24 bladder cancer cells. T24 bladder cancer cells were transfected with small interfering RNA against FAK (siFAK) or control (conRNA). (A and B) The expression of FAK was examined using western blotting. (C and D) Cell apoptosis was examined using (C) deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay and (D) Annexin V/propidium iodide. (E) Cell survival was examined using an MTT assay. Scale bar, 200 µm. *P

Article Snippet: The treated cells were stained with FITC, Annexin V and propidium iodide (PI), and the stained cells were analyzed using a FACSort™ flow cytometer (Becton-Dickinson, Franklin Lakes, NJ, USA) and evaluated with the CellQuest™ software system (BD Biosciences).

Techniques: Transfection, Small Interfering RNA, Expressing, Western Blot, End Labeling, MTT Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Inhibition of focal adhesion kinase induces apoptosis in bladder cancer cells via Src and the phosphatidylinositol 3-kinase/Akt pathway

doi: 10.3892/etm.2015.2745

Figure Lengend Snippet: Src is an important mediator of FAK-regulated apoptosis in T24 bladder cancer cells. T24 bladder cancer cells were treated with PP2. (A and B) Cell apoptosis was examined using (A) deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay and (B) Annexin V/propidium iodide. (C and D) The expression of FAK, pFAK, Src and pSrc was examined using western blotting. Scale bar, 200 µm. FAK, focal adhesion kinase; pFAK, phosphorylated FAK; TGFβ, transforming growth factor-β.

Article Snippet: The treated cells were stained with FITC, Annexin V and propidium iodide (PI), and the stained cells were analyzed using a FACSort™ flow cytometer (Becton-Dickinson, Franklin Lakes, NJ, USA) and evaluated with the CellQuest™ software system (BD Biosciences).

Techniques: End Labeling, Expressing, Western Blot

Journal: Experimental and Therapeutic Medicine

Article Title: Inhibition of focal adhesion kinase induces apoptosis in bladder cancer cells via Src and the phosphatidylinositol 3-kinase/Akt pathway

doi: 10.3892/etm.2015.2745

Figure Lengend Snippet: Suppression of FAK phosphorylation induces apoptosis in T24 bladder cancer cells. T24 bladder cancer cells were treated with PF-228 and 5 ng/ml TGFβ. (A and B) Cell apoptosis was examined using (A) deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay and (B) Annexin V/propidium iodide. (C and D) The expression of FAK, pFAK, Src, pSrc, Akt, pAkt, caspase-3 and c-caspase-3 was examined using western blotting. (E) Cell survival was examined using an MTT assay. Scale bar, 200 µm. *P

Article Snippet: The treated cells were stained with FITC, Annexin V and propidium iodide (PI), and the stained cells were analyzed using a FACSort™ flow cytometer (Becton-Dickinson, Franklin Lakes, NJ, USA) and evaluated with the CellQuest™ software system (BD Biosciences).

Techniques: End Labeling, Expressing, Western Blot, MTT Assay