Journal: Molecular Cancer

Article Title: Exosome-mediated secretion of LOXL4 promotes hepatocellular carcinoma cell invasion and metastasis

doi: 10.1186/s12943-019-0948-8

Figure Lengend Snippet: LOXL4 expression is upregulated in HCC tissues and predicts a poor clinical outcome. a The mRNA expression level of LOXL4 in HCC tissues compared with the normal liver tissues (N) revealed using the GSE6764 dataset. b-c The mRNA expression level of LOXL4 in the non-tumorous liver (NT) and HCC tissues revealed using the GSE36376 ( b ) and GSE84402 ( c ) datasets. d The mRNA expression level of LOXL4 in 42 matched NT and HCC tissues derived from Ren Ji cohort detected by qRT-PCR. e IHC staining performed using an antibody against LOXL4 and representative photographs of the LOXL4 staining in NT and HCC tissues. (Scale bar: 100 μm). f LOXL4 expression was upregulated in 175 HCC tissues compared with NT tissues (T > N). g Comparison of overall survival of HCC patients with different LOXL4 protein expression. h Comparison of disease-free survival of HCC patients with different LOXL4 protein expression. i Overall survival analysis of HCC patients in TCGA cohort. j-k Forest plot showing the association between LOXL4 expression and HCC survival using univariate ( j ) and multivariate ( k ) analyses. (HR, hazard ratio; CI, confidence interval)

Article Snippet: Quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was isolated from HCC tissues and cell lines using TRIzol RNA isolation reagent (Takara, Japan) and reverse transcribed using a PrimeScript qRT-PCR kit (Takara, Japan) according to the manufacturer’s instructions.

Techniques: Expressing, Derivative Assay, Quantitative RT-PCR, Immunohistochemistry, Staining

Journal: Virology Journal

Article Title: miR-124 attenuates Japanese encephalitis virus replication by targeting DNM2

doi: 10.1186/s12985-016-0562-y

Figure Lengend Snippet: Expression of miR-124 and DNM2 in PK15 cells during JEV infection. a PK15 cells were infected with JEV. Mock-infected (M) or virus-infected (V) cells were harvested at the indicated times, and the miR-124 and DNM2 RNA levels were quantified using qRT-PCR. The data were normalized to the levels measured in mock-infected cells to provide relative expression levels (V/M). The results shown are means ± SD with triplicate data points. b Replicate cell cultures were evaluated by western blot for DNM2 protein expression. β-actin serves as a loading control

Article Snippet: The synthesis of cDNA was conducted with 1 μg of total RNA using an RT-PCR reagent kit (PrimeScript™ 1st Strand cDNA Synthesis Kit, TaKaRa Biotechnology, DaLian, China) with an oligo-dT primer for mRNA and a special stem-loop primer for miRNAs [ ].

Techniques: Expressing, Infection, Quantitative RT-PCR, Western Blot