primerstar hs dna polymerase Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    TaKaRa primestar high sensitivity hs dna polymerase
    Characterization of <t>DNA</t> analogs. ( A ) PCR assays analyzed by 5% native polyacrylamide gel electrophoresis. Total PCR volume 100 µl: 20 ng 418-bp DNA template (pJ1506), 0.4 mM each LJM-3222 (5'-G TA CGC AG T ) and LJM-3223 (5'-TGTGAGT AGCTCACTCAT AG ), 0.2 mM each dNTP with indicated analog triphosphate ( 1–9 ) completely replacing appropriate dNTP, and 5 U DNA polymerase (indicated with plus symbol) with associated buffer and cycle conditions. Taq DNA polymerase ( Taq ) conditions: Taq DNA polymerase buffer with 100 mg/ml BSA and 2 mM MgCl ; 98°C (3 min), 30 cycles of [94°C (30 s), 60°C (30 s), and 72°C (45 s)], 72°C (5 min). <t>PrimeSTAR</t> HS DNA polymerase (PS) conditions: PrimeSTAR GC buffer with 2 M betaine; 98°C (3 min), 30 cycles of [98°C (15 s), 60°C (5 s), and 72°C (45 s)], 72°C (5 min). Pwo SuperYield DNA Polymerase ( Pwo ) conditions: Pwo PCR buffer with GC-rich solution and 2 M betaine; 98°C (3 min), 30 cycles of [98°C (1 min), 60°C (2 min), and 72°C (8 min)], 72°C (5 min). Lane 1 is marker (M) DNA (100 bp DNA ladder, Invitrogen) with 400 - and 500-bp bands indicated. ( B ) Anion exchange chromatography of 98-bp DNA-like polymers (pJ1923). Following equilibration in 20 mM Tris–HCl, pH 8 (buffer A), samples were eluted over 25 min at a 1 ml/min flow rate in a linear gradient from 50 to 100% buffer B (buffer A plus 1 M NaCl). Eluent absorbance at 260 nm (milli-absorbance units) was monitored with elution time (min).
    Primestar High Sensitivity Hs Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primestar high sensitivity hs dna polymerase/product/TaKaRa
    Average 99 stars, based on 134 article reviews
    Price from $9.99 to $1999.99
    primestar high sensitivity hs dna polymerase - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Characterization of DNA analogs. ( A ) PCR assays analyzed by 5% native polyacrylamide gel electrophoresis. Total PCR volume 100 µl: 20 ng 418-bp DNA template (pJ1506), 0.4 mM each LJM-3222 (5'-G TA CGC AG T ) and LJM-3223 (5'-TGTGAGT AGCTCACTCAT AG ), 0.2 mM each dNTP with indicated analog triphosphate ( 1–9 ) completely replacing appropriate dNTP, and 5 U DNA polymerase (indicated with plus symbol) with associated buffer and cycle conditions. Taq DNA polymerase ( Taq ) conditions: Taq DNA polymerase buffer with 100 mg/ml BSA and 2 mM MgCl ; 98°C (3 min), 30 cycles of [94°C (30 s), 60°C (30 s), and 72°C (45 s)], 72°C (5 min). PrimeSTAR HS DNA polymerase (PS) conditions: PrimeSTAR GC buffer with 2 M betaine; 98°C (3 min), 30 cycles of [98°C (15 s), 60°C (5 s), and 72°C (45 s)], 72°C (5 min). Pwo SuperYield DNA Polymerase ( Pwo ) conditions: Pwo PCR buffer with GC-rich solution and 2 M betaine; 98°C (3 min), 30 cycles of [98°C (1 min), 60°C (2 min), and 72°C (8 min)], 72°C (5 min). Lane 1 is marker (M) DNA (100 bp DNA ladder, Invitrogen) with 400 - and 500-bp bands indicated. ( B ) Anion exchange chromatography of 98-bp DNA-like polymers (pJ1923). Following equilibration in 20 mM Tris–HCl, pH 8 (buffer A), samples were eluted over 25 min at a 1 ml/min flow rate in a linear gradient from 50 to 100% buffer B (buffer A plus 1 M NaCl). Eluent absorbance at 260 nm (milli-absorbance units) was monitored with elution time (min).

    Journal: Nucleic Acids Research

    Article Title: Mechanical properties of DNA-like polymers

    doi: 10.1093/nar/gkt808

    Figure Lengend Snippet: Characterization of DNA analogs. ( A ) PCR assays analyzed by 5% native polyacrylamide gel electrophoresis. Total PCR volume 100 µl: 20 ng 418-bp DNA template (pJ1506), 0.4 mM each LJM-3222 (5'-G TA CGC AG T ) and LJM-3223 (5'-TGTGAGT AGCTCACTCAT AG ), 0.2 mM each dNTP with indicated analog triphosphate ( 1–9 ) completely replacing appropriate dNTP, and 5 U DNA polymerase (indicated with plus symbol) with associated buffer and cycle conditions. Taq DNA polymerase ( Taq ) conditions: Taq DNA polymerase buffer with 100 mg/ml BSA and 2 mM MgCl ; 98°C (3 min), 30 cycles of [94°C (30 s), 60°C (30 s), and 72°C (45 s)], 72°C (5 min). PrimeSTAR HS DNA polymerase (PS) conditions: PrimeSTAR GC buffer with 2 M betaine; 98°C (3 min), 30 cycles of [98°C (15 s), 60°C (5 s), and 72°C (45 s)], 72°C (5 min). Pwo SuperYield DNA Polymerase ( Pwo ) conditions: Pwo PCR buffer with GC-rich solution and 2 M betaine; 98°C (3 min), 30 cycles of [98°C (1 min), 60°C (2 min), and 72°C (8 min)], 72°C (5 min). Lane 1 is marker (M) DNA (100 bp DNA ladder, Invitrogen) with 400 - and 500-bp bands indicated. ( B ) Anion exchange chromatography of 98-bp DNA-like polymers (pJ1923). Following equilibration in 20 mM Tris–HCl, pH 8 (buffer A), samples were eluted over 25 min at a 1 ml/min flow rate in a linear gradient from 50 to 100% buffer B (buffer A plus 1 M NaCl). Eluent absorbance at 260 nm (milli-absorbance units) was monitored with elution time (min).

    Article Snippet: For analogs 3 , 4 , 5 , 6 and 9 , PCR reactions (100 µl) included 20 ng template, 0.4 mM forward and reverse primers, PrimeSTAR GC buffer (Takara), 0.2 mM each dNTP (again with dTTP completely replaced with analog, except for variant 9 , which completely replaced dATP), 2 M betaine (Sigma-Aldrich) and 5 U PrimeSTAR HS DNA polymerase (Takara).

    Techniques: Polymerase Chain Reaction, Polyacrylamide Gel Electrophoresis, Marker, Chromatography, Flow Cytometry

    mRNA of traA gene analysis. (A) Northern hybridization for traA mRNA and 16S rRNA using the total RNA of LKG/pASK-IBA3plus; left, Anc(C)/pASK-IBA3plus; middle, M54(C)/pASK-IBA3plus; right. The upper and lower figures are X-ray films of traA mRNA and 16S rRNA, respectively. The asterisks (*) indicate the three signals (one weak and two strong). (B) RT-PCR for Anc(C)/pASK-IBA3plus to determine the 5′- and 3′-terminal sequences of traA mRNA. Lambda DNA digested with Sty I was used as a molecular size marker; lane M. RT-PCR products for determination of the 5′-terminus. The three bands were designated as (i), (ii), and (iii), respectively; lane 1. RT-PCR products for determination of the 3′-terminus. The two bands were designated as (iv) and (v), respectively; lane 2. (C) Schematic representations of the start and end positions of traA mRNA. The vertical lines represent the positions of 5′- and 3′-terminal sequences of (i)–(v) shown in (B) . The gray and black boxes represent the positions of traA_r2 and traA_f primer annealing sites in RT-PCR. The arrow represents the position of the traA1 probe annealing site for Northern hybridization.

    Journal: Frontiers in Microbiology

    Article Title: Characterization of a single mutation in TraQ in a strain of Escherichia coli partially resistant to Qβ infection

    doi: 10.3389/fmicb.2015.00124

    Figure Lengend Snippet: mRNA of traA gene analysis. (A) Northern hybridization for traA mRNA and 16S rRNA using the total RNA of LKG/pASK-IBA3plus; left, Anc(C)/pASK-IBA3plus; middle, M54(C)/pASK-IBA3plus; right. The upper and lower figures are X-ray films of traA mRNA and 16S rRNA, respectively. The asterisks (*) indicate the three signals (one weak and two strong). (B) RT-PCR for Anc(C)/pASK-IBA3plus to determine the 5′- and 3′-terminal sequences of traA mRNA. Lambda DNA digested with Sty I was used as a molecular size marker; lane M. RT-PCR products for determination of the 5′-terminus. The three bands were designated as (i), (ii), and (iii), respectively; lane 1. RT-PCR products for determination of the 3′-terminus. The two bands were designated as (iv) and (v), respectively; lane 2. (C) Schematic representations of the start and end positions of traA mRNA. The vertical lines represent the positions of 5′- and 3′-terminal sequences of (i)–(v) shown in (B) . The gray and black boxes represent the positions of traA_r2 and traA_f primer annealing sites in RT-PCR. The arrow represents the position of the traA1 probe annealing site for Northern hybridization.

    Article Snippet: PCR was performed using the resultant cDNA as the template, PrimeSTAR®; HS DNA polymerase (Takara Bio Inc., Shiga, Japan), and the primers pACYC_rev2 and traA_r2.

    Techniques: Northern Blot, Hybridization, Reverse Transcription Polymerase Chain Reaction, Lambda DNA Preparation, Marker