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  • 99
    Thermo Fisher total rna
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 471882 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher trizol reagent
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 635164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen rneasy mini kit
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 346422 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher rna
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 177224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher mgcl2
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Mgcl2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 104037 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher taq polymerase
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 33062 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher superscript iii reverse transcriptase
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Superscript Iii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 90655 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher superscript ii reverse transcriptase
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Superscript Ii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 93822 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher sybr green pcr master mix
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 101787 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher high capacity complementary dna reverse transcription kit
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    High Capacity Complementary Dna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 377 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen qiaquick pcr purification kit
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Qiaquick Pcr Purification Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 137129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher first strand cdna
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    First Strand Cdna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 84046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Bio-Rad iq sybr green supermix
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Iq Sybr Green Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 72789 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad iscript cdna synthesis kit
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Iscript Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 95548 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    iscript cdna synthesis kit - by Bioz Stars, 2020-11
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    99
    Thermo Fisher power sybr green pcr master mix
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Power Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 69800 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Bio-Rad real time pcr
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Real Time Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 12747 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega pgem t easy vector
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Pgem T Easy Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 91767 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher lipofectamine 2000
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Lipofectamine 2000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 466214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa primescript rt reagent kit
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Primescript Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 72258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen qiaquick gel extraction kit
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
    Qiaquick Gel Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 113321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rna  (Promega)
    99
    Promega rna
    Comparisons of the signal intensities of <t>miRNA</t> ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each <t>RNA.</t> Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.
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    Comparisons of the signal intensities of miRNA ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each RNA. Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Double In situ Hybridization for MicroRNAs and mRNAs in Brain Tissues

    doi: 10.3389/fnmol.2016.00126

    Figure Lengend Snippet: Comparisons of the signal intensities of miRNA ISH in fresh and fixed brain sections with or without proteinase K digestion . In situ detection of miR-34a (A) and miR-181a (B) was performed in adult mouse brain sections. The hybridization temperature was 37°C below the T m of each RNA. Detection was carried out using the NBT/BCIP colorimetric method. The sections were treated with or without proteinase K at the indicated concentrations. (A) Representative images of miR-34a in fresh (upper panels) and PFA perfusion-fixed (lower panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the TRN. Scale bars in (A) , 1 mm; 0.2 mm in the inserted images. (B) ISH signals for miR-181a in fresh (left panels) and PFA perfusion-fixed (right panels) brain sections are shown. The insert images are a higher magnification of the boxed brain sections including the hippocampal DG (B) . Scale bars in (B) , 500 μm; 100 μm in the inserted images.

    Article Snippet: Total RNA (10 ng for miRNA, 200 ng for mRNA) was reverse transcribed using a TaqMan™ MicroRNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA) for miRNA or a Moloney murine leukemia virus reverse transcriptase (Invitrogen, Carlsbad, CA) for mRNA.

    Techniques: In Situ Hybridization, In Situ, Hybridization, RNA Detection