Journal: Journal of Radiation Research
Article Title: Nicaraven mitigates radiation-induced lung injury by downregulating the NF-κB and TGF-β/Smad pathways to suppress the inflammatory response
Figure Lengend Snippet: The DNA damage of lung tissue cells at the acute phase after treatments. (A) Representative confocal images show the expression of Sca-1 and γ-H2AX in lung tissue cells. Quantitative data on the number of Sca-1 + stem cells (B), the total cells with γ-H2AX foci formation (C), and the Sca-1 + stem cells with γ-H2AX foci formation (D, Arrows) are shown. Scale bars: 10 μm. The nuclei were stained with DAPI. Data are represented as means ± SD. **p < 0.01 vs CON group, †p < 0.05, ††p < 0.01 vs IR group. CON: Control, IR: Radiation, IR + N: Radiation+Nicaraven.
Article Snippet: After antigen retrieval and blocking, sections were incubated with rabbit anti-mouse γ-H2AX antibody (1:400 dilution, Abcam) and rat anti-mouse Sca-1 antibody (1:200 dilution, Abcam) overnight at 4°C, and followed by the appropriate fluorescent-conjugated secondary antibodies at 25°C for 60 min.
Techniques: Expressing, Staining