primary antibodies against ift88 Search Results


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  • 86
    WuXi AppTec antibody against ift88
    Primer sequences.
    Antibody Against Ift88, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against ift88/product/WuXi AppTec
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody against ift88 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology rabbit antibodies against ift88
    Primer sequences.
    Rabbit Antibodies Against Ift88, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antibodies against ift88/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antibodies against ift88 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    Proteintech rabbit polyclonal antibody against ift88
    Primer sequences.
    Rabbit Polyclonal Antibody Against Ift88, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibody against ift88/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal antibody against ift88 - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Primer sequences.

    Journal: Molecular Medicine Reports

    Article Title: Role of IFT88 in icariin-regulated maintenance of the chondrocyte phenotype

    doi: 10.3892/mmr.2018.8486

    Figure Lengend Snippet: Primer sequences.

    Article Snippet: Using standard immunofluorescence methods, the primary cilia were stained with anti-acetylated α-tubulin antibody (cat. no. T7451; 1:300 dilution; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) or double-stained with that antibody together with an antibody against IFT88 (cat. no. AP11138b; 1:50 dilution; Abgent Inc., San Diego, CA, USA).

    Techniques:

    Icariin promotes ciliary assembly and IFT88 expression. (A) Primary cilia were stained for acetylated-α-tubulin (red coloration, denoted by the white arrows; magnification, ×200). (B) The histograms reveal that, compared with the control group, 10 µmol/l icariin increased primary ciliary assembly (Control group, 27.91±9.95% cf. Icariin, 34.06%±10.06; *P<0.05), and icariin moderately increased the ciliary length from 3.07±0.74 to 3.34±1.34 µm. (C) Icariin increased IFT88 gene expression, peaking at 10 µmol/l (*P<0.05 cf. 0 µmol/l icariin). (D) Icariin upregulated production of the ciliary protein, IFT88, in a concentration- and time-dependent manner (*P<0.05 cf. 0 µmol/l icariin, or treatment at 0 h). IFT88, intraflagellar transport protein 88.

    Journal: Molecular Medicine Reports

    Article Title: Role of IFT88 in icariin-regulated maintenance of the chondrocyte phenotype

    doi: 10.3892/mmr.2018.8486

    Figure Lengend Snippet: Icariin promotes ciliary assembly and IFT88 expression. (A) Primary cilia were stained for acetylated-α-tubulin (red coloration, denoted by the white arrows; magnification, ×200). (B) The histograms reveal that, compared with the control group, 10 µmol/l icariin increased primary ciliary assembly (Control group, 27.91±9.95% cf. Icariin, 34.06%±10.06; *P<0.05), and icariin moderately increased the ciliary length from 3.07±0.74 to 3.34±1.34 µm. (C) Icariin increased IFT88 gene expression, peaking at 10 µmol/l (*P<0.05 cf. 0 µmol/l icariin). (D) Icariin upregulated production of the ciliary protein, IFT88, in a concentration- and time-dependent manner (*P<0.05 cf. 0 µmol/l icariin, or treatment at 0 h). IFT88, intraflagellar transport protein 88.

    Article Snippet: Using standard immunofluorescence methods, the primary cilia were stained with anti-acetylated α-tubulin antibody (cat. no. T7451; 1:300 dilution; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) or double-stained with that antibody together with an antibody against IFT88 (cat. no. AP11138b; 1:50 dilution; Abgent Inc., San Diego, CA, USA).

    Techniques: Expressing, Staining, Concentration Assay

    Icariin enhances primary ciliary assembly and maintains the phenotype of the primary chondrocytes. (A) Primary cilia were stained for acetylated-α-tubulin (red). Functional IFT88 (green) was widely dispersed in the cytoplasm, although it was principally centralized along the ciliary axonemes, which also contained acetylated α-tubulin (denoted by the white arrows; magnification, ×200). (B) Icariin increased the proportion of primary chondrocytes with cilia, from 29.54±8.24 to 38.48±10.36%, and led to a slight elongation of the cilia from 2.48±0.85 to 2.65±0.63 µm (*P<0.05). (C) Toluidine blue staining revealed that icariin promoted cartilage matrix secretion by primary chondrocytes. (D) Expression of phenotype-associated genes in primary chondrocytes (*P<0.05). IFT88, intraflagellar transport protein 88.

    Journal: Molecular Medicine Reports

    Article Title: Role of IFT88 in icariin-regulated maintenance of the chondrocyte phenotype

    doi: 10.3892/mmr.2018.8486

    Figure Lengend Snippet: Icariin enhances primary ciliary assembly and maintains the phenotype of the primary chondrocytes. (A) Primary cilia were stained for acetylated-α-tubulin (red). Functional IFT88 (green) was widely dispersed in the cytoplasm, although it was principally centralized along the ciliary axonemes, which also contained acetylated α-tubulin (denoted by the white arrows; magnification, ×200). (B) Icariin increased the proportion of primary chondrocytes with cilia, from 29.54±8.24 to 38.48±10.36%, and led to a slight elongation of the cilia from 2.48±0.85 to 2.65±0.63 µm (*P<0.05). (C) Toluidine blue staining revealed that icariin promoted cartilage matrix secretion by primary chondrocytes. (D) Expression of phenotype-associated genes in primary chondrocytes (*P<0.05). IFT88, intraflagellar transport protein 88.

    Article Snippet: Using standard immunofluorescence methods, the primary cilia were stained with anti-acetylated α-tubulin antibody (cat. no. T7451; 1:300 dilution; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) or double-stained with that antibody together with an antibody against IFT88 (cat. no. AP11138b; 1:50 dilution; Abgent Inc., San Diego, CA, USA).

    Techniques: Staining, Functional Assay, Expressing

    Icariin regulates IFT88 expression via the ERK signalling pathway. (A) Quantitative polymerase chain reaction measuring the expression levels of the IFT88 and various phenotype-associated genes after transformation of IFT88 siRNA (*P<0.05 compared with the control group; ## P<0.01 compared with the icariin group). (B) IFT88 knockdown reduced the expression of phosphorylated ERK (**P<0.01 compared with the siCon group). (C) Icariin promoted expression of IFT88 and phosphorylated ERK, and slightly increased ERK phosphorylation, after transformation of IFT88 siRNA (**P<0.01 compared with the control group). (D) An ERK inhibitor reduced IFT88 expression. Icariin upregulated IFT88 expression, but could not restore such expression in the presence of the ERK inhibitor, PD0325901 (*P<0.05; **P<0.01 compared with the control group). ERK, extracellular signal-regulated kinase; IFT88, intraflagellar transport protein 88; Ica, icariin; PD, PD0325901, Con, control; T-ERK, total ERK; P-ERK, phosphorylated ERK.

    Journal: Molecular Medicine Reports

    Article Title: Role of IFT88 in icariin-regulated maintenance of the chondrocyte phenotype

    doi: 10.3892/mmr.2018.8486

    Figure Lengend Snippet: Icariin regulates IFT88 expression via the ERK signalling pathway. (A) Quantitative polymerase chain reaction measuring the expression levels of the IFT88 and various phenotype-associated genes after transformation of IFT88 siRNA (*P<0.05 compared with the control group; ## P<0.01 compared with the icariin group). (B) IFT88 knockdown reduced the expression of phosphorylated ERK (**P<0.01 compared with the siCon group). (C) Icariin promoted expression of IFT88 and phosphorylated ERK, and slightly increased ERK phosphorylation, after transformation of IFT88 siRNA (**P<0.01 compared with the control group). (D) An ERK inhibitor reduced IFT88 expression. Icariin upregulated IFT88 expression, but could not restore such expression in the presence of the ERK inhibitor, PD0325901 (*P<0.05; **P<0.01 compared with the control group). ERK, extracellular signal-regulated kinase; IFT88, intraflagellar transport protein 88; Ica, icariin; PD, PD0325901, Con, control; T-ERK, total ERK; P-ERK, phosphorylated ERK.

    Article Snippet: Using standard immunofluorescence methods, the primary cilia were stained with anti-acetylated α-tubulin antibody (cat. no. T7451; 1:300 dilution; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) or double-stained with that antibody together with an antibody against IFT88 (cat. no. AP11138b; 1:50 dilution; Abgent Inc., San Diego, CA, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Transformation Assay