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  • 99
    Lonza 24 well tissue culture plates
    24 Well Tissue Culture Plates, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher ack lysis buffer
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    Miltenyi Biotec ls columns
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    Miltenyi Biotec anti ly 6g microbead kit mouse
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    Miltenyi Biotec cd34 microbead kit human
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    Copley Scientific citdas evaluation software version 2 0
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    Becton Dickinson fluorescence based flow cytometry
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Fluorescence Based Flow Cytometry, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 89/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Miltenyi Biotec ms columns
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Ms Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rlt buffer
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Rlt Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 17414 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Carl Roth GmbH 26 mm glass microscope slides
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
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    93
    Carl Roth GmbH 60 mm cover glasses
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    60 Mm Cover Glasses, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Applied BioPhysics 8w10e pet microelectrode arrays
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    8w10e Pet Microelectrode Arrays, supplied by Applied BioPhysics, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Cayman Chemical 9 12 octadecadiynioc acid
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    9 12 Octadecadiynioc Acid, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher alexa fluor 488 goat anti rat igg h l
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Alexa Fluor 488 Goat Anti Rat Igg H L, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Miltenyi Biotec automacs running buffer macs separation buffer
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Automacs Running Buffer Macs Separation Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 200 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fetal bovine serum dialyzed
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Fetal Bovine Serum Dialyzed, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher fetal calf serum
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Fetal Calf Serum, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4315 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bca method
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Bca Method, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 9513 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti ret pe
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Anti Ret Pe, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti h2b
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Anti H2b, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson anti cd8α
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Anti Cd8α, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 1383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher anti cd117 apc
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Anti Cd117 Apc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti alpha tubulin rabbit antibody 600 401 880s
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Anti Alpha Tubulin Rabbit Antibody 600 401 880s, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend anti mouse ccr4
    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow <t>cytometry</t> ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p
    Anti Mouse Ccr4, supplied by BioLegend, used in various techniques. Bioz Stars score: 88/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam anti brg1 antibody
    Structure and gel analysis of hBRG1. A , sequence of the ATPase, i.e. residues 726–1249 from <t>BRG1</t> isoform 2 (Uniprot-ID P51532-2). The ATP-binding domain ( light blue ) and the helicase C-terminal domain ( dark blue ) are highlighted, and conserved blocks ( cons. bl. ) are indicated. The highly conserved Gln 758 and Lys 785 residues are printed in bold. B , homology model of the ATP-binding domain. The localization of ATPase motifs is indicated according to the color scheme shown on the right . ATPγS ( black sticks ) is docked into the ATP-binding site. C , a magnified view on the ATP-binding site, showing the orientation of the Q ( violet ) and I ( red ) motifs toward ATPγS. Gln 758 , Lys 785 , and ATPγS are shown as sticks , representing nitrogen ( blue ), sulfur ( yellow ), phosphorus ( orange ), oxygen ( red ), hydrogen ( white ), and carbon ( green ) atoms. Green dashed lines indicate hydrogen bonds between Gln 758 and N 6/7 of the nucleotide, and light blue lines indicate putative contacts between Lys 785 and the phosphates of the nucleotide, which are presumably mediated by a Mg 2+ ). The length of the respective bonds, including the hydrogen and the putative hydrogen acceptor, is listed. D, SDS-PAGE of BRG1 proteins. 1 μg of BRG1 proteins (wtBRG1, lanes 4 and 6 ; and mutants, lanes 1–3 and 7–10 ) were loaded on a 6% SDS-PAGE gel and stained with Coomassie (protein size marker, M , lane 5 ).
    Anti Brg1 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow cytometry ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p

    Journal: Toxins

    Article Title: Triggering Apoptotic Death of Human Epidermal Keratinocytes by Malic Acid: Involvement of Endoplasmic Reticulum Stress- and Mitochondria-Dependent Signaling Pathways

    doi: 10.3390/toxins7010081

    Figure Lengend Snippet: Malic acid induced cells’ morphological changes and decreased the number of total viable human keratinocytes (HaCaT cells). Cells were incubated with or without 15 mM of malic acid for 6, 12, 24, and 48 h, and then were examined and photographed by a phase-contrast microscope ( A ) and were harvested for determination the percentage of viable cells by flow cytometry ( B ). Data are presented as means ± S.D. of the results from three independent experiments ( * p

    Article Snippet: Fluorescence-based flow cytometry was performed using a Becton Dickinson FACSCalibur® (BD Biosciences, Totowa, NJ, USA) flow cytometer equipped with a 488 nm laser.

    Techniques: Incubation, Microscopy, Flow Cytometry, Cytometry

    Increase of oxidative stress and anaerobic glycolysis in HaCaT cells treated with malic acid. ( A ) Malic acid increased mitochondrial ROS production in HaCaT cells detected by flow cytometry; ( B ) malic acid increased ROS production in terms of increasing 2',7'-dichlorodihydrofluorescein (DCF) fluorescence intensity in HaCaT cells detected by flow cytometry; ( C ) The mean values of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured in real-time by a Seahorse XF24 Analyzer. Data are presented as means ± S.D. of the results from three independent experiments ( ** p

    Journal: Toxins

    Article Title: Triggering Apoptotic Death of Human Epidermal Keratinocytes by Malic Acid: Involvement of Endoplasmic Reticulum Stress- and Mitochondria-Dependent Signaling Pathways

    doi: 10.3390/toxins7010081

    Figure Lengend Snippet: Increase of oxidative stress and anaerobic glycolysis in HaCaT cells treated with malic acid. ( A ) Malic acid increased mitochondrial ROS production in HaCaT cells detected by flow cytometry; ( B ) malic acid increased ROS production in terms of increasing 2',7'-dichlorodihydrofluorescein (DCF) fluorescence intensity in HaCaT cells detected by flow cytometry; ( C ) The mean values of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured in real-time by a Seahorse XF24 Analyzer. Data are presented as means ± S.D. of the results from three independent experiments ( ** p

    Article Snippet: Fluorescence-based flow cytometry was performed using a Becton Dickinson FACSCalibur® (BD Biosciences, Totowa, NJ, USA) flow cytometer equipped with a 488 nm laser.

    Techniques: Flow Cytometry, Cytometry, Fluorescence

    Structure and gel analysis of hBRG1. A , sequence of the ATPase, i.e. residues 726–1249 from BRG1 isoform 2 (Uniprot-ID P51532-2). The ATP-binding domain ( light blue ) and the helicase C-terminal domain ( dark blue ) are highlighted, and conserved blocks ( cons. bl. ) are indicated. The highly conserved Gln 758 and Lys 785 residues are printed in bold. B , homology model of the ATP-binding domain. The localization of ATPase motifs is indicated according to the color scheme shown on the right . ATPγS ( black sticks ) is docked into the ATP-binding site. C , a magnified view on the ATP-binding site, showing the orientation of the Q ( violet ) and I ( red ) motifs toward ATPγS. Gln 758 , Lys 785 , and ATPγS are shown as sticks , representing nitrogen ( blue ), sulfur ( yellow ), phosphorus ( orange ), oxygen ( red ), hydrogen ( white ), and carbon ( green ) atoms. Green dashed lines indicate hydrogen bonds between Gln 758 and N 6/7 of the nucleotide, and light blue lines indicate putative contacts between Lys 785 and the phosphates of the nucleotide, which are presumably mediated by a Mg 2+ ). The length of the respective bonds, including the hydrogen and the putative hydrogen acceptor, is listed. D, SDS-PAGE of BRG1 proteins. 1 μg of BRG1 proteins (wtBRG1, lanes 4 and 6 ; and mutants, lanes 1–3 and 7–10 ) were loaded on a 6% SDS-PAGE gel and stained with Coomassie (protein size marker, M , lane 5 ).

    Journal: The Journal of Biological Chemistry

    Article Title: Elucidation of the functional roles of the Q and I motifs in the human chromatin-remodeling enzyme BRG1

    doi: 10.1074/jbc.RA118.005685

    Figure Lengend Snippet: Structure and gel analysis of hBRG1. A , sequence of the ATPase, i.e. residues 726–1249 from BRG1 isoform 2 (Uniprot-ID P51532-2). The ATP-binding domain ( light blue ) and the helicase C-terminal domain ( dark blue ) are highlighted, and conserved blocks ( cons. bl. ) are indicated. The highly conserved Gln 758 and Lys 785 residues are printed in bold. B , homology model of the ATP-binding domain. The localization of ATPase motifs is indicated according to the color scheme shown on the right . ATPγS ( black sticks ) is docked into the ATP-binding site. C , a magnified view on the ATP-binding site, showing the orientation of the Q ( violet ) and I ( red ) motifs toward ATPγS. Gln 758 , Lys 785 , and ATPγS are shown as sticks , representing nitrogen ( blue ), sulfur ( yellow ), phosphorus ( orange ), oxygen ( red ), hydrogen ( white ), and carbon ( green ) atoms. Green dashed lines indicate hydrogen bonds between Gln 758 and N 6/7 of the nucleotide, and light blue lines indicate putative contacts between Lys 785 and the phosphates of the nucleotide, which are presumably mediated by a Mg 2+ ). The length of the respective bonds, including the hydrogen and the putative hydrogen acceptor, is listed. D, SDS-PAGE of BRG1 proteins. 1 μg of BRG1 proteins (wtBRG1, lanes 4 and 6 ; and mutants, lanes 1–3 and 7–10 ) were loaded on a 6% SDS-PAGE gel and stained with Coomassie (protein size marker, M , lane 5 ).

    Article Snippet: The following reagents were used: mCherry antibody (orb66657 biorybt); rat anti-DYKDDDDK antibody (200474, Agilent Technologies); anti-BRG1 antibody (ab1110641, Abcam); anti–α-tubulin (rabbit) antibody (600-401-880S, Rockland); anti-H2B (07-371, Upstate-Millipore); mouse anti-p53 antibody (sc-126, Santa Cruz (DO-1)); peroxidase-conjugated AffiniPure goat anti-rabbit IgG (H+L) (111-035-144, Jackson ImmunoResearch); peroxidase-conjugated AffiniPure goat anti-mouse IgG (H+L) (111-035-146, Jackson ImmunoResearch); Alexa Fluor® 488 goat anti-rat IgG (H+L) (A-11006, Invitrogen or Thermo Fisher Scientific); streptavidin, immobilized on agarose CL-4B (Fluka/Sigma–Aldrich); ATP ultrapure (Jena Bioscience); ADP ultrapure (Cell Technologies); ADPγS (Jena Bioscience); [γ-35 S]ATP (PerkinElmer); [γ-32 P]ATP (Hartmann Analytic); NuPAGE 4–12% Bis-Tris gels (Thermo Fisher Scientific); anti-FLAG M2-agarose beads (Sigma–Aldrich); DAPI (Sigma–Aldrich); Amicon Ultra-4 centrifugal filter units 500 μl of 10,000 molecular mass cutoff (Merck Millipore); FuGENE® HD transfection kit (Promega); complete EDTA-free protease inhibitor mixture (Roche); benzonase (E1014-5KU, Sigma); glass coverslips (Ø = 12 mm) (Roth); 24-mm × 60-mm cover glasses (Roth); 76-mm × 26-mm glass microscope slides (Roth); PEI-cellulose F plates (Merck Millipore); Amersham Biosciences Protran premium nitrocellulose blotting membrane 0.45-μm pore size (GE Healthcare); PVDF Immobilon-P transfer membrane 0.45-μm pore size (Merck Millipore); Super Signal West Dura Extended Duration Substrate (Thermo Fisher Scientific), NativePAGE 4–16% Bis-Tris protein gels (Thermo Fisher Scientific); Sf-900TM II SFM (1×) medium (Thermo Fisher); Dulbecco's modified Eagle's medium, low glucose [1 g/L], GlutaMAXTM -I, medium (Thermo Fisher); penicillin/streptomycin (10,000 units/ml) (Gibco–Thermo Fisher Scientific); 0.5% trypsin-EDTA (10×) (Gibco– Thermo Fisher Scientific); fetal calf serum (Gibco); DNase I (Roche); Hoechst 33342 (Sigma–Aldrich); G418 (Sigma–Aldrich); 1× PBS (Ca2+ and Mg2+ free) (Gibco); 1 m HEPES (Gibco); fetal bovine serum (dialyzed) (Thermo Fisher); Partec CellTrics® filter (50 μm) (Partec); polystyrene round-bottomed tubes (3.5 ml, 55 mm × 12 mm) (Sarstedt); polypropylene tubes (5 ml; 75 mm × 12 mm) (Fisher Scientific, part of Thermo Fisher Scientific); 8W10E+ PET microelectrode arrays (Applied BioPhysics); 30-μm preseparation filter (MACS; Miltenyi Biotec).

    Techniques: Sequencing, Binding Assay, SDS Page, Staining, Marker