prep cell apparatus Search Results


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  • 93
    Thermo Fisher ase 100 apparatus
    Ase 100 Apparatus, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ase 100 apparatus/product/Thermo Fisher
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    ase 100 apparatus - by Bioz Stars, 2020-07
    93/100 stars
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    95
    Bio-Rad prep cell apparatus
    Prep Cell Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prep cell apparatus/product/Bio-Rad
    Average 95 stars, based on 305 article reviews
    Price from $9.99 to $1999.99
    prep cell apparatus - by Bioz Stars, 2020-07
    95/100 stars
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    85
    Bio-Rad mini prep cell apparatus
    Mini Prep Cell Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mini prep cell apparatus/product/Bio-Rad
    Average 85 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    mini prep cell apparatus - by Bioz Stars, 2020-07
    85/100 stars
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    85
    Valiant fast prep 24 cell disruption apparatus
    Fast Prep 24 Cell Disruption Apparatus, supplied by Valiant, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast prep 24 cell disruption apparatus/product/Valiant
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    fast prep 24 cell disruption apparatus - by Bioz Stars, 2020-07
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    85
    Bio-Rad prep cell apparatus run
    Prep Cell Apparatus Run, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prep cell apparatus run/product/Bio-Rad
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    prep cell apparatus run - by Bioz Stars, 2020-07
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    85
    Bio-Rad preparative sds page apparatus
    Glycosylation of SfManI. Sf9 cells were infected with a baculovirus expression vector encoding SfManI, labeled with tran- 35 S-label, and total intracellular proteins were extracted at 28 h after infection. Some cultures were treated with 1.5 μg of tunicamycin per ml of medium (lanes 3 and 4). SfManI was immunoprecipitated from the extracts with an anti-SfManI antiserum, and immunoprecipitates were treated with either EndoH f (lanes 2 and 4) or buffer alone (lanes 1 and 3). The samples were then analyzed by <t>SDS–PAGE</t> and autoradiography. The positions and sizes (kDa) of protein markers are shown on the left-hand side of the figure and the position of SfManI is shown on the right.
    Preparative Sds Page Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preparative sds page apparatus/product/Bio-Rad
    Average 85 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    preparative sds page apparatus - by Bioz Stars, 2020-07
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    88
    BioSpec fast prep apparatus
    Glycosylation of SfManI. Sf9 cells were infected with a baculovirus expression vector encoding SfManI, labeled with tran- 35 S-label, and total intracellular proteins were extracted at 28 h after infection. Some cultures were treated with 1.5 μg of tunicamycin per ml of medium (lanes 3 and 4). SfManI was immunoprecipitated from the extracts with an anti-SfManI antiserum, and immunoprecipitates were treated with either EndoH f (lanes 2 and 4) or buffer alone (lanes 1 and 3). The samples were then analyzed by <t>SDS–PAGE</t> and autoradiography. The positions and sizes (kDa) of protein markers are shown on the left-hand side of the figure and the position of SfManI is shown on the right.
    Fast Prep Apparatus, supplied by BioSpec, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fast prep apparatus/product/BioSpec
    Average 88 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    fast prep apparatus - by Bioz Stars, 2020-07
    88/100 stars
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    84
    Bio-Rad model 491 prep cell device
    Glycosylation of SfManI. Sf9 cells were infected with a baculovirus expression vector encoding SfManI, labeled with tran- 35 S-label, and total intracellular proteins were extracted at 28 h after infection. Some cultures were treated with 1.5 μg of tunicamycin per ml of medium (lanes 3 and 4). SfManI was immunoprecipitated from the extracts with an anti-SfManI antiserum, and immunoprecipitates were treated with either EndoH f (lanes 2 and 4) or buffer alone (lanes 1 and 3). The samples were then analyzed by <t>SDS–PAGE</t> and autoradiography. The positions and sizes (kDa) of protein markers are shown on the left-hand side of the figure and the position of SfManI is shown on the right.
    Model 491 Prep Cell Device, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/model 491 prep cell device/product/Bio-Rad
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    model 491 prep cell device - by Bioz Stars, 2020-07
    84/100 stars
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    85
    Bio-Rad micropreparative continuous elution sds page
    Glycosylation of SfManI. Sf9 cells were infected with a baculovirus expression vector encoding SfManI, labeled with tran- 35 S-label, and total intracellular proteins were extracted at 28 h after infection. Some cultures were treated with 1.5 μg of tunicamycin per ml of medium (lanes 3 and 4). SfManI was immunoprecipitated from the extracts with an anti-SfManI antiserum, and immunoprecipitates were treated with either EndoH f (lanes 2 and 4) or buffer alone (lanes 1 and 3). The samples were then analyzed by <t>SDS–PAGE</t> and autoradiography. The positions and sizes (kDa) of protein markers are shown on the left-hand side of the figure and the position of SfManI is shown on the right.
    Micropreparative Continuous Elution Sds Page, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/micropreparative continuous elution sds page/product/Bio-Rad
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    micropreparative continuous elution sds page - by Bioz Stars, 2020-07
    85/100 stars
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    99
    Thermo Fisher slide a lyzer mini dialysis devices
    Glycosylation of SfManI. Sf9 cells were infected with a baculovirus expression vector encoding SfManI, labeled with tran- 35 S-label, and total intracellular proteins were extracted at 28 h after infection. Some cultures were treated with 1.5 μg of tunicamycin per ml of medium (lanes 3 and 4). SfManI was immunoprecipitated from the extracts with an anti-SfManI antiserum, and immunoprecipitates were treated with either EndoH f (lanes 2 and 4) or buffer alone (lanes 1 and 3). The samples were then analyzed by <t>SDS–PAGE</t> and autoradiography. The positions and sizes (kDa) of protein markers are shown on the left-hand side of the figure and the position of SfManI is shown on the right.
    Slide A Lyzer Mini Dialysis Devices, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 439 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/slide a lyzer mini dialysis devices/product/Thermo Fisher
    Average 99 stars, based on 439 article reviews
    Price from $9.99 to $1999.99
    slide a lyzer mini dialysis devices - by Bioz Stars, 2020-07
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    88
    GE Healthcare ettan ipgphor ii apparatus
    Glycosylation of SfManI. Sf9 cells were infected with a baculovirus expression vector encoding SfManI, labeled with tran- 35 S-label, and total intracellular proteins were extracted at 28 h after infection. Some cultures were treated with 1.5 μg of tunicamycin per ml of medium (lanes 3 and 4). SfManI was immunoprecipitated from the extracts with an anti-SfManI antiserum, and immunoprecipitates were treated with either EndoH f (lanes 2 and 4) or buffer alone (lanes 1 and 3). The samples were then analyzed by <t>SDS–PAGE</t> and autoradiography. The positions and sizes (kDa) of protein markers are shown on the left-hand side of the figure and the position of SfManI is shown on the right.
    Ettan Ipgphor Ii Apparatus, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ettan ipgphor ii apparatus/product/GE Healthcare
    Average 88 stars, based on 91 article reviews
    Price from $9.99 to $1999.99
    ettan ipgphor ii apparatus - by Bioz Stars, 2020-07
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    99
    Thermo Fisher neon transfection device
    Increased association of LFA-1 with the cytoskeleton upon Cer formation. ( A ) Dual colour images of lifeact-GFP transfected monocytes and SPT of LFA-1 nanoclusters (red trajectories) in control (left) and SMase (right) treated cells. After <t>transfection</t> with lifeact-GFP and, if applicable treatment with SMase, monocytes were labeled with TS2/4-Atto647N and seeded on fibronectin. Movies were recorded at 100ms/frame in TIRF mode. Custom-written software was employed to quantify and normalize the actin signal in each cell by assigning the lowest actin intensity to 0 (blue) and the highest to 1 (yellow), as indicated in the color code (bottom). White dotted lines represent the cell boundaries. Within the control cell, selected area i) represents an example of a low actin signal, while selected area ii) is an example of a high actin signal in control cells, superimposed with the corresponding LFA-1 trajectories (red). Both selected areas are shown as enlarged images next to the control cell. (B ) Distribution of the normalized actin intensity associated with each LFA-1 trajectory in unperturbed (blue) or SMase treated cells (green). Each histogram contains at least 140 trajectories taken from 15 cells in multiple experiments. The data represents the mean ± SD obtained after bootstrapping of actin intensities measured in all experiments. The inset shows the difference between the normalized frequency of localizations of LFA-1 trajectories in unperturbed cells and upon SMase treatment as function of the normalized actin signal. P-values were compared to unperturbed cells by 2way ANOVA with Bonferroni post-test, ***
    Neon Transfection Device, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/neon transfection device/product/Thermo Fisher
    Average 99 stars, based on 119 article reviews
    Price from $9.99 to $1999.99
    neon transfection device - by Bioz Stars, 2020-07
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    Image Search Results


    Glycosylation of SfManI. Sf9 cells were infected with a baculovirus expression vector encoding SfManI, labeled with tran- 35 S-label, and total intracellular proteins were extracted at 28 h after infection. Some cultures were treated with 1.5 μg of tunicamycin per ml of medium (lanes 3 and 4). SfManI was immunoprecipitated from the extracts with an anti-SfManI antiserum, and immunoprecipitates were treated with either EndoH f (lanes 2 and 4) or buffer alone (lanes 1 and 3). The samples were then analyzed by SDS–PAGE and autoradiography. The positions and sizes (kDa) of protein markers are shown on the left-hand side of the figure and the position of SfManI is shown on the right.

    Journal: Insect biochemistry and molecular biology

    Article Title: Biosynthesis and subcellular localization of a lepidopteran insect alpha 1,2-mannosidase

    doi:

    Figure Lengend Snippet: Glycosylation of SfManI. Sf9 cells were infected with a baculovirus expression vector encoding SfManI, labeled with tran- 35 S-label, and total intracellular proteins were extracted at 28 h after infection. Some cultures were treated with 1.5 μg of tunicamycin per ml of medium (lanes 3 and 4). SfManI was immunoprecipitated from the extracts with an anti-SfManI antiserum, and immunoprecipitates were treated with either EndoH f (lanes 2 and 4) or buffer alone (lanes 1 and 3). The samples were then analyzed by SDS–PAGE and autoradiography. The positions and sizes (kDa) of protein markers are shown on the left-hand side of the figure and the position of SfManI is shown on the right.

    Article Snippet: Soluble proteins were extracted and the SfManI fragment was purified by size fractionation on a preparative SDS–PAGE apparatus (Prep Cell; Bio-Rad Laboratories, Hercules, CA).

    Techniques: Infection, Expressing, Plasmid Preparation, Labeling, Immunoprecipitation, SDS Page, Autoradiography

    Analysis of SfManI N-glycosylation mutants. Sf9 cells were infected with baculovirus vectors encoding wild type SfManI (WT), the knockout mutant (ΔN), or the enhanced N-glycosylation mutant (eN). The cells were labeled with tran- 35 S-label and total intracellular proteins were extracted at 28 h after infection. SfManI was immunop-recipitated from the extracts with an anti-SfManI antiserum and immunoprecipitates were treated with either EndoH f (+) or buffer alone (−). The samples were then analyzed by SDS–PAGE and autoradiography. The positions and sizes (kDa) of protein markers are shown on the left-hand side of the figure and the position of SfManI is shown on the right.

    Journal: Insect biochemistry and molecular biology

    Article Title: Biosynthesis and subcellular localization of a lepidopteran insect alpha 1,2-mannosidase

    doi:

    Figure Lengend Snippet: Analysis of SfManI N-glycosylation mutants. Sf9 cells were infected with baculovirus vectors encoding wild type SfManI (WT), the knockout mutant (ΔN), or the enhanced N-glycosylation mutant (eN). The cells were labeled with tran- 35 S-label and total intracellular proteins were extracted at 28 h after infection. SfManI was immunop-recipitated from the extracts with an anti-SfManI antiserum and immunoprecipitates were treated with either EndoH f (+) or buffer alone (−). The samples were then analyzed by SDS–PAGE and autoradiography. The positions and sizes (kDa) of protein markers are shown on the left-hand side of the figure and the position of SfManI is shown on the right.

    Article Snippet: Soluble proteins were extracted and the SfManI fragment was purified by size fractionation on a preparative SDS–PAGE apparatus (Prep Cell; Bio-Rad Laboratories, Hercules, CA).

    Techniques: Infection, Knock-Out, Mutagenesis, Labeling, SDS Page, Autoradiography

    Expression of SfManI-GFP and unfused GFP. Sf9 cells were infected with wild type baculovirus (lane 1), a baculovirus vector encoding unfused GFP (lane 2), or a baculovirus vector encoding SfManI-GFP (lane 3). Total intracellular protein extracts were prepared at 24 h post infection and analyzed by SDS–PAGE and immunoblotting with a polyclonal antiserum against GFP. The positions and sizes (kDa) of protein markers are shown on the left-hand side of the figure and the positions of GFP and SfManI-GFP are shown on the right.

    Journal: Insect biochemistry and molecular biology

    Article Title: Biosynthesis and subcellular localization of a lepidopteran insect alpha 1,2-mannosidase

    doi:

    Figure Lengend Snippet: Expression of SfManI-GFP and unfused GFP. Sf9 cells were infected with wild type baculovirus (lane 1), a baculovirus vector encoding unfused GFP (lane 2), or a baculovirus vector encoding SfManI-GFP (lane 3). Total intracellular protein extracts were prepared at 24 h post infection and analyzed by SDS–PAGE and immunoblotting with a polyclonal antiserum against GFP. The positions and sizes (kDa) of protein markers are shown on the left-hand side of the figure and the positions of GFP and SfManI-GFP are shown on the right.

    Article Snippet: Soluble proteins were extracted and the SfManI fragment was purified by size fractionation on a preparative SDS–PAGE apparatus (Prep Cell; Bio-Rad Laboratories, Hercules, CA).

    Techniques: Expressing, Infection, Plasmid Preparation, SDS Page

    Increased association of LFA-1 with the cytoskeleton upon Cer formation. ( A ) Dual colour images of lifeact-GFP transfected monocytes and SPT of LFA-1 nanoclusters (red trajectories) in control (left) and SMase (right) treated cells. After transfection with lifeact-GFP and, if applicable treatment with SMase, monocytes were labeled with TS2/4-Atto647N and seeded on fibronectin. Movies were recorded at 100ms/frame in TIRF mode. Custom-written software was employed to quantify and normalize the actin signal in each cell by assigning the lowest actin intensity to 0 (blue) and the highest to 1 (yellow), as indicated in the color code (bottom). White dotted lines represent the cell boundaries. Within the control cell, selected area i) represents an example of a low actin signal, while selected area ii) is an example of a high actin signal in control cells, superimposed with the corresponding LFA-1 trajectories (red). Both selected areas are shown as enlarged images next to the control cell. (B ) Distribution of the normalized actin intensity associated with each LFA-1 trajectory in unperturbed (blue) or SMase treated cells (green). Each histogram contains at least 140 trajectories taken from 15 cells in multiple experiments. The data represents the mean ± SD obtained after bootstrapping of actin intensities measured in all experiments. The inset shows the difference between the normalized frequency of localizations of LFA-1 trajectories in unperturbed cells and upon SMase treatment as function of the normalized actin signal. P-values were compared to unperturbed cells by 2way ANOVA with Bonferroni post-test, ***

    Journal: Scientific Reports

    Article Title: Changes in membrane sphingolipid composition modulate dynamics and adhesion of integrin nanoclusters

    doi: 10.1038/srep20693

    Figure Lengend Snippet: Increased association of LFA-1 with the cytoskeleton upon Cer formation. ( A ) Dual colour images of lifeact-GFP transfected monocytes and SPT of LFA-1 nanoclusters (red trajectories) in control (left) and SMase (right) treated cells. After transfection with lifeact-GFP and, if applicable treatment with SMase, monocytes were labeled with TS2/4-Atto647N and seeded on fibronectin. Movies were recorded at 100ms/frame in TIRF mode. Custom-written software was employed to quantify and normalize the actin signal in each cell by assigning the lowest actin intensity to 0 (blue) and the highest to 1 (yellow), as indicated in the color code (bottom). White dotted lines represent the cell boundaries. Within the control cell, selected area i) represents an example of a low actin signal, while selected area ii) is an example of a high actin signal in control cells, superimposed with the corresponding LFA-1 trajectories (red). Both selected areas are shown as enlarged images next to the control cell. (B ) Distribution of the normalized actin intensity associated with each LFA-1 trajectory in unperturbed (blue) or SMase treated cells (green). Each histogram contains at least 140 trajectories taken from 15 cells in multiple experiments. The data represents the mean ± SD obtained after bootstrapping of actin intensities measured in all experiments. The inset shows the difference between the normalized frequency of localizations of LFA-1 trajectories in unperturbed cells and upon SMase treatment as function of the normalized actin signal. P-values were compared to unperturbed cells by 2way ANOVA with Bonferroni post-test, ***

    Article Snippet: Sample preparation for dual colour imaging and SPT experiments For dual colour single molecule imaging of LFA-1 together with actin, THP-1 cells were transfected with lifeact-GFP by electroporation using the Neon® Transfection device (Invitrogen) and transfection materials (Invitrogen) according to manufacturer´s instructions.

    Techniques: Transfection, Single-particle Tracking, Labeling, Software