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    Thermo Fisher transfection complexes hsa mir 335 5p pre mir mirna precursor
    Migration activity of hMSCs after <t>miR-335</t> <t>transfection.</t> ( A ) hMSCs were transfected with miR/PEI/MNP complexes and migration activity was tested 24 h after transfection. The overgrown surface area of transfected cells was measured before and 12 h after scratching. Untransfected cells were used as control. Data are presented as mean ± standard error, n = 5, ** p ≤ 0.001; ( B , C ) Representative images after transfection with magnetic polyplexes containing either scrambled miR ( B , B′ ) or miR-335 ( C , C′ ). Images were taken immediately after ( B , C ) and 12 h after scratching ( B′ , C′ ). Values represent the non-overgrown surface area. Scale bar = 200 μm.
    Transfection Complexes Hsa Mir 335 5p Pre Mir Mirna Precursor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfection complexes hsa mir 335 5p pre mir mirna precursor/product/Thermo Fisher
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    93
    GenePharma Company pre prepare sirna sirna mate complex
    Migration activity of hMSCs after <t>miR-335</t> <t>transfection.</t> ( A ) hMSCs were transfected with miR/PEI/MNP complexes and migration activity was tested 24 h after transfection. The overgrown surface area of transfected cells was measured before and 12 h after scratching. Untransfected cells were used as control. Data are presented as mean ± standard error, n = 5, ** p ≤ 0.001; ( B , C ) Representative images after transfection with magnetic polyplexes containing either scrambled miR ( B , B′ ) or miR-335 ( C , C′ ). Images were taken immediately after ( B , C ) and 12 h after scratching ( B′ , C′ ). Values represent the non-overgrown surface area. Scale bar = 200 μm.
    Pre Prepare Sirna Sirna Mate Complex, supplied by GenePharma Company, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pre prepare sirna sirna mate complex/product/GenePharma Company
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    pre prepare sirna sirna mate complex - by Bioz Stars, 2020-11
    93/100 stars
      Buy from Supplier

    Image Search Results


    Migration activity of hMSCs after miR-335 transfection. ( A ) hMSCs were transfected with miR/PEI/MNP complexes and migration activity was tested 24 h after transfection. The overgrown surface area of transfected cells was measured before and 12 h after scratching. Untransfected cells were used as control. Data are presented as mean ± standard error, n = 5, ** p ≤ 0.001; ( B , C ) Representative images after transfection with magnetic polyplexes containing either scrambled miR ( B , B′ ) or miR-335 ( C , C′ ). Images were taken immediately after ( B , C ) and 12 h after scratching ( B′ , C′ ). Values represent the non-overgrown surface area. Scale bar = 200 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Innovative Strategy for MicroRNA Delivery in Human Mesenchymal Stem Cells via Magnetic Nanoparticles

    doi: 10.3390/ijms140610710

    Figure Lengend Snippet: Migration activity of hMSCs after miR-335 transfection. ( A ) hMSCs were transfected with miR/PEI/MNP complexes and migration activity was tested 24 h after transfection. The overgrown surface area of transfected cells was measured before and 12 h after scratching. Untransfected cells were used as control. Data are presented as mean ± standard error, n = 5, ** p ≤ 0.001; ( B , C ) Representative images after transfection with magnetic polyplexes containing either scrambled miR ( B , B′ ) or miR-335 ( C , C′ ). Images were taken immediately after ( B , C ) and 12 h after scratching ( B′ , C′ ). Values represent the non-overgrown surface area. Scale bar = 200 μm.

    Article Snippet: Fluorescent Labeling of Transfection Complexes hsa-miR-335-5p Pre-miR™ miRNA Precursor (Ambion) was labeled with Cy™5 dye using Label IT® miRNA Labeling Kit, Version 2 (Mirus Bio LLC, Madison, WI, USA) according to the manufacturer’s protocol.

    Techniques: Migration, Activity Assay, Transfection

    Efficient knockdown of miR-335 target genes. ( A , B ) hMSCs were transfected with miR/PEI or miR/PEI/MNP complexes and relative gene expression of TNC ( A ) and RUNX2 ( B ) was measured by real-time PCR 5, 24 and 72 h after transfection. Cells treated with miR only were used as control. Dashed line indicates gene expression in untransfected cells. Values were normalized to GAPDH expression and are represented as mean ± standard error, n = 5, * p ≤ 0.05 versus miR, ** p ≤ 0.001 versus miR, ## p ≤ 0.001 versus miR/PEI - mediated transfection.

    Journal: International Journal of Molecular Sciences

    Article Title: Innovative Strategy for MicroRNA Delivery in Human Mesenchymal Stem Cells via Magnetic Nanoparticles

    doi: 10.3390/ijms140610710

    Figure Lengend Snippet: Efficient knockdown of miR-335 target genes. ( A , B ) hMSCs were transfected with miR/PEI or miR/PEI/MNP complexes and relative gene expression of TNC ( A ) and RUNX2 ( B ) was measured by real-time PCR 5, 24 and 72 h after transfection. Cells treated with miR only were used as control. Dashed line indicates gene expression in untransfected cells. Values were normalized to GAPDH expression and are represented as mean ± standard error, n = 5, * p ≤ 0.05 versus miR, ** p ≤ 0.001 versus miR, ## p ≤ 0.001 versus miR/PEI - mediated transfection.

    Article Snippet: Fluorescent Labeling of Transfection Complexes hsa-miR-335-5p Pre-miR™ miRNA Precursor (Ambion) was labeled with Cy™5 dye using Label IT® miRNA Labeling Kit, Version 2 (Mirus Bio LLC, Madison, WI, USA) according to the manufacturer’s protocol.

    Techniques: Transfection, Expressing, Real-time Polymerase Chain Reaction

    Processing of transfected precursor-miR. ( A ) hMSCs were transfected with precursor-miR-335 using miR/PEI or miR/PEI/MNP complexes and level of a mature miR-335 strand was detected by real time PCR 5, 24 and 72 h after transfection. Cells treated with miR only were used as a control. Dashed line indicates miR-335 expression in untransfected cells. Right plot shows a linear scale of miR-335 expression 72 h after transfection. Values were normalized to RNU6B expression and represented as mean ± standard error. The data are representative of 5 independent biological experiments ( n = 5), each of which was measured in qPCR-triplicates. ** p ≤ 0.001 versus miR, ## p ≤ 0.001 versus miR/PEI-mediated transfection; ( B , C ) Labeled miR/PEI ( B ) and miR/PEI/MNP complexes ( C ) were visualized by confocal laser scanning microscopy 72 h after transfection in hMSCs. miR-335 was labeled with Cy™5 dye (cyan), PEI was labeled with Oregon Green ® 488 (yellow) and MNPs were labeled with Atto 565 (red). Nuclei were counterstained with DAPI (gray). The arrow indicates condensed miR/PEI complexes inside the nucleus. Scale bar = 5 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Innovative Strategy for MicroRNA Delivery in Human Mesenchymal Stem Cells via Magnetic Nanoparticles

    doi: 10.3390/ijms140610710

    Figure Lengend Snippet: Processing of transfected precursor-miR. ( A ) hMSCs were transfected with precursor-miR-335 using miR/PEI or miR/PEI/MNP complexes and level of a mature miR-335 strand was detected by real time PCR 5, 24 and 72 h after transfection. Cells treated with miR only were used as a control. Dashed line indicates miR-335 expression in untransfected cells. Right plot shows a linear scale of miR-335 expression 72 h after transfection. Values were normalized to RNU6B expression and represented as mean ± standard error. The data are representative of 5 independent biological experiments ( n = 5), each of which was measured in qPCR-triplicates. ** p ≤ 0.001 versus miR, ## p ≤ 0.001 versus miR/PEI-mediated transfection; ( B , C ) Labeled miR/PEI ( B ) and miR/PEI/MNP complexes ( C ) were visualized by confocal laser scanning microscopy 72 h after transfection in hMSCs. miR-335 was labeled with Cy™5 dye (cyan), PEI was labeled with Oregon Green ® 488 (yellow) and MNPs were labeled with Atto 565 (red). Nuclei were counterstained with DAPI (gray). The arrow indicates condensed miR/PEI complexes inside the nucleus. Scale bar = 5 μm.

    Article Snippet: Fluorescent Labeling of Transfection Complexes hsa-miR-335-5p Pre-miR™ miRNA Precursor (Ambion) was labeled with Cy™5 dye using Label IT® miRNA Labeling Kit, Version 2 (Mirus Bio LLC, Madison, WI, USA) according to the manufacturer’s protocol.

    Techniques: Transfection, Real-time Polymerase Chain Reaction, Expressing, Labeling, Confocal Laser Scanning Microscopy