Journal: Scientific Reports
Article Title: Identification of a membrane-less compartment regulating invadosome function and motility
Figure Lengend Snippet: Liprin-α1 depletion does not affect invadosome formation, but affects their motility. ( A,C,E ) NIH-Src cells co-transfected with siRNAs and GFP-LifeAct (invadosomes), fixed before ( A , T 0 ) or after incubation with nocodazole and PP2 ( C , T 60 ), and after washout and incubation for 5 min to rescue invadosomes ( E , R 5 ). Immunostaining for GFP-LifeAct (green), tubulin (red), and liprin-α1 (blue). Bars, 20 µm. ( B,D,F ) Graphs: top, mean percentage of cells with/without invadosomes; bottom, invadosome organization: cells classified based on the highest organization of their invadosomes (n = 186–309). χ 2 test: no differences with liprin-α1 and control siRNA; *** p
Article Snippet: 327 was a gift from S. Courtneidge Alexa-488, Alexa-568 and Alexa-647 secondary Abs and Oregon 488-gelatin (Life Technologies, Paisley, Scotland, UK); FITC- and TRITC-conjugated phalloidin (Sigma-Aldrich, St. Louis, MO); goat-anti rabbit nanogold–conjugated secondary Ab (Nanoprobes, Stony Brook, New York), fibronectin (BD Biosciences, San Jose, CA); peroxidase-conjugated anti-rabbit and anti-mouse secondary Abs, and Enhanced Chemiluminescence (ECL) Detection System (Amersham Biosciences, Little Chalfont, UK); Peroxidase-conjugated anti-hamster secondary Ab (Life Technologies); nocodazole and poly-L-lysine hydrobromid (Sigma-Aldrich, St. Louis, MO); PP2 Src kinase inhibitor (Calbiochem) and G418 (Merck, Darmstadt, Germany).
Techniques: Transfection, Incubation, Immunostaining