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  • 99
    Thermo Fisher power sybr green pcr master mix
    Transcript levels of the “readthrough” acetylcholinesterase (AChE-R) variant are increased in cerebral cortex of AD subjects Relative mRNA levels of the transcripts for AChE-T (or “tailed”), AChE-R (or “readthrought”) and N-AChE (or N-extended) splice variants and for proline-rich membrane anchor 1 (PRiMA-1) were analysed by q <t>RT-PCR</t> in frontal cortex of NDC (n= 22) and AD subjects (n= 19). For AChE transcript analysis specific primers with Power <t>SYBR®</t> Green PCR Master Mix were employed and the specificity of the PCR products was confirmed by dissociation curve analysis. PRiMA-1 transcripts were measured using a specific TaqMan GenExpression Assay with TaqMan PCR Master Mix. Transcript levels were calculated by the comparative 2 −ΔCt method with respect to GAPDH. The results were confirmed in two independent determinations. Mean value ± SEM are represented. *Significantly increased ( p
    Power Sybr Green Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 70411 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/power sybr green pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 70411 article reviews
    Price from $9.99 to $1999.99
    power sybr green pcr master mix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher sybr green power pcr master mix
    Transcript levels of the “readthrough” acetylcholinesterase (AChE-R) variant are increased in cerebral cortex of AD subjects Relative mRNA levels of the transcripts for AChE-T (or “tailed”), AChE-R (or “readthrought”) and N-AChE (or N-extended) splice variants and for proline-rich membrane anchor 1 (PRiMA-1) were analysed by q <t>RT-PCR</t> in frontal cortex of NDC (n= 22) and AD subjects (n= 19). For AChE transcript analysis specific primers with Power <t>SYBR®</t> Green PCR Master Mix were employed and the specificity of the PCR products was confirmed by dissociation curve analysis. PRiMA-1 transcripts were measured using a specific TaqMan GenExpression Assay with TaqMan PCR Master Mix. Transcript levels were calculated by the comparative 2 −ΔCt method with respect to GAPDH. The results were confirmed in two independent determinations. Mean value ± SEM are represented. *Significantly increased ( p
    Sybr Green Power Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green power pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 112 article reviews
    Price from $9.99 to $1999.99
    sybr green power pcr master mix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher power sybr green pcr master mix kit
    Transcript levels of the “readthrough” acetylcholinesterase (AChE-R) variant are increased in cerebral cortex of AD subjects Relative mRNA levels of the transcripts for AChE-T (or “tailed”), AChE-R (or “readthrought”) and N-AChE (or N-extended) splice variants and for proline-rich membrane anchor 1 (PRiMA-1) were analysed by q <t>RT-PCR</t> in frontal cortex of NDC (n= 22) and AD subjects (n= 19). For AChE transcript analysis specific primers with Power <t>SYBR®</t> Green PCR Master Mix were employed and the specificity of the PCR products was confirmed by dissociation curve analysis. PRiMA-1 transcripts were measured using a specific TaqMan GenExpression Assay with TaqMan PCR Master Mix. Transcript levels were calculated by the comparative 2 −ΔCt method with respect to GAPDH. The results were confirmed in two independent determinations. Mean value ± SEM are represented. *Significantly increased ( p
    Power Sybr Green Pcr Master Mix Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1797 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/power sybr green pcr master mix kit/product/Thermo Fisher
    Average 92 stars, based on 1797 article reviews
    Price from $9.99 to $1999.99
    power sybr green pcr master mix kit - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

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    Transcript levels of the “readthrough” acetylcholinesterase (AChE-R) variant are increased in cerebral cortex of AD subjects Relative mRNA levels of the transcripts for AChE-T (or “tailed”), AChE-R (or “readthrought”) and N-AChE (or N-extended) splice variants and for proline-rich membrane anchor 1 (PRiMA-1) were analysed by q RT-PCR in frontal cortex of NDC (n= 22) and AD subjects (n= 19). For AChE transcript analysis specific primers with Power SYBR® Green PCR Master Mix were employed and the specificity of the PCR products was confirmed by dissociation curve analysis. PRiMA-1 transcripts were measured using a specific TaqMan GenExpression Assay with TaqMan PCR Master Mix. Transcript levels were calculated by the comparative 2 −ΔCt method with respect to GAPDH. The results were confirmed in two independent determinations. Mean value ± SEM are represented. *Significantly increased ( p

    Journal: Journal of Alzheimer's disease : JAD

    Article Title: Increased expression of readthrough acetylcholinesterase variants in the brains of Alzheimer's disease patients

    doi: 10.3233/JAD-160220

    Figure Lengend Snippet: Transcript levels of the “readthrough” acetylcholinesterase (AChE-R) variant are increased in cerebral cortex of AD subjects Relative mRNA levels of the transcripts for AChE-T (or “tailed”), AChE-R (or “readthrought”) and N-AChE (or N-extended) splice variants and for proline-rich membrane anchor 1 (PRiMA-1) were analysed by q RT-PCR in frontal cortex of NDC (n= 22) and AD subjects (n= 19). For AChE transcript analysis specific primers with Power SYBR® Green PCR Master Mix were employed and the specificity of the PCR products was confirmed by dissociation curve analysis. PRiMA-1 transcripts were measured using a specific TaqMan GenExpression Assay with TaqMan PCR Master Mix. Transcript levels were calculated by the comparative 2 −ΔCt method with respect to GAPDH. The results were confirmed in two independent determinations. Mean value ± SEM are represented. *Significantly increased ( p

    Article Snippet: First-strand cDNAs were synthesized by reverse transcription of 1.5 μg of total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems; Life Technologies Paisley, UK), according to the manufacturer's instructions. q RT-PCR amplification was performed using StepOne-Plus™ Real-Time PCR System with Power SYBR® Green PCR Master Mix (Applied Biosystems) according to the manufacturer's instructions for analysis of AChE transcripts.

    Techniques: Variant Assay, Reverse Transcription Polymerase Chain Reaction, SYBR Green Assay, Polymerase Chain Reaction

    Standardization of qPCR assays for parasite load quantitation from skin lesions of golden hamsters infected with Leishmania ( V .) braziliensis. Golden hamsters were infected with 10 5 Leishmania ( V .) braziliensis promastigotes, for 110 days. The panel shows representative amplification plots with the fluorescent signal magnitude for parasite kDNA and golden hamster GAPDH targets ( a ) and melting curve indicating the reaction specificity, observed through a single peak in each curve ( b ) Standard curves for parasite kDNA and golden hamster GAPDH targets indicate the dynamic extension, PCR efficiency and linearity coefficient of the reaction ( c ) To run the experiments, DNA and RNA were extracted from the same sample and qPCR assays were performed using SYBR Green fluorophore, as described in Methods section

    Journal: Parasites & Vectors

    Article Title: Development of real-time PCR assays for evaluation of immune response and parasite load in golden hamster (Mesocricetus auratus) infected by Leishmania (Viannia) braziliensis

    doi: 10.1186/s13071-016-1647-6

    Figure Lengend Snippet: Standardization of qPCR assays for parasite load quantitation from skin lesions of golden hamsters infected with Leishmania ( V .) braziliensis. Golden hamsters were infected with 10 5 Leishmania ( V .) braziliensis promastigotes, for 110 days. The panel shows representative amplification plots with the fluorescent signal magnitude for parasite kDNA and golden hamster GAPDH targets ( a ) and melting curve indicating the reaction specificity, observed through a single peak in each curve ( b ) Standard curves for parasite kDNA and golden hamster GAPDH targets indicate the dynamic extension, PCR efficiency and linearity coefficient of the reaction ( c ) To run the experiments, DNA and RNA were extracted from the same sample and qPCR assays were performed using SYBR Green fluorophore, as described in Methods section

    Article Snippet: For real-time PCR assays, 2 μl cDNA (at 10 ng/μl) were used in a final reaction volume of 10 μl, with 5.0 μl of Power SYBR Green PCR Master Mix 2X (Life Technologies, CA, USA), 100 nM of forward and 100 nM of reverse primers (except for the IL-4 primers, where 200 nM of Forward and Reverse primers were used), in a ViiA7 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA), in 384 well plates.

    Techniques: Real-time Polymerase Chain Reaction, Quantitation Assay, Infection, Amplification, Polymerase Chain Reaction, SYBR Green Assay