Journal: Analytica chimica acta
Article Title: Integrated preservation and sample clean up procedures for studying water ingestion by recreational swimmers via urinary biomarker determination
Figure Lengend Snippet: Comparison of isocratic and gradient HPLC analyses of cyanuric acid in urine. The concentration of cyanuric acid in the chromatogram is 2.15 mg/L Sample pre-treatment included acid precipitation of proteins and, for illustrative purposes, only the C 18 cartridge was used (section 2.2, except the elution solvent was de-ionized water instead of 0.05 M hydrochloric acid). A volume of 20 μL of solution was injected into the HPLC with UV detection at 213 nm. A 10 × 0.3 cm PGC column was used. For the isocratic separation, the mobile phase was 95:5 (%, v/v) 50 mM K2HPO4 buffer (pH 9.1): methanol at 0.6 mL/min flow rate. For the gradient elution, conditions were the same except that the methanol content was ramped to 100% after 5 min and held for 40 min.
Article Snippet: Cyanuric acid (98%), metaphosphoric acid (99.99%), K2HPO4 (98+%), perchloric acid (60%, v/v), formic acid (96%, v/v), 1.0 M hydrochloric acid, 1.0 M sodium hydroxide, HPLC grade methanol and methylene chloride were purchased from Aldrich Chemical (Milwaukee, WI).
Techniques: High Performance Liquid Chromatography, Concentration Assay, Injection, Pyrolysis Gas Chromatography, Flow Cytometry