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  • 90
    Nanoprobes au nanoprobes post amplification labeling
    Detection sensitivity of the developed LAMP-SERS assay. (a) Electrophoresis patterns of LAMP amplification products generated from different concentrations of S. Enteritidis DNA template. LAMP reaction was performed using 10-fold serial dilutions of S. Enteritidis DNA template (6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL). M: 1-kb DNA ladder marker; NC: negative control (without target DNA template). (b) SERS spectral features of <t>Au-nanoprobes</t> incubated with LAMP products of different concentrations of S. Enteritidis DNA template. SERS peak intensity centered at 583 cm -1 was used for quantitative analysis of S. Enteritidis. Variations in SERS peak intensity centered at 583 cm -1 were as a function of S. Enteritidis concentration. The inset shows a linear relationship in the concentration range from 6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL. Error bars are standard deviations from a total of three independent measurements. Raman spectroscopic analysis was performed using a 785-nm laser for excitation at 25 mW and 10 sec as integration time. The raw SERS spectra were baseline corrected and the final spectrum was obtained by averaging three different measurements without spectral normalization.
    Au Nanoprobes Post Amplification Labeling, supplied by Nanoprobes, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 4 article reviews
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    89
    Agilent technologies post amplification
    Detection sensitivity of the developed LAMP-SERS assay. (a) Electrophoresis patterns of LAMP amplification products generated from different concentrations of S. Enteritidis DNA template. LAMP reaction was performed using 10-fold serial dilutions of S. Enteritidis DNA template (6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL). M: 1-kb DNA ladder marker; NC: negative control (without target DNA template). (b) SERS spectral features of <t>Au-nanoprobes</t> incubated with LAMP products of different concentrations of S. Enteritidis DNA template. SERS peak intensity centered at 583 cm -1 was used for quantitative analysis of S. Enteritidis. Variations in SERS peak intensity centered at 583 cm -1 were as a function of S. Enteritidis concentration. The inset shows a linear relationship in the concentration range from 6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL. Error bars are standard deviations from a total of three independent measurements. Raman spectroscopic analysis was performed using a 785-nm laser for excitation at 25 mW and 10 sec as integration time. The raw SERS spectra were baseline corrected and the final spectrum was obtained by averaging three different measurements without spectral normalization.
    Post Amplification, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/post amplification/product/Agilent technologies
    Average 89 stars, based on 28 article reviews
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    90
    Nanoprobes postamplification labeling step
    Detection sensitivity of the developed LAMP-SERS assay. (a) Electrophoresis patterns of LAMP amplification products generated from different concentrations of S. Enteritidis DNA template. LAMP reaction was performed using 10-fold serial dilutions of S. Enteritidis DNA template (6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL). M: 1-kb DNA ladder marker; NC: negative control (without target DNA template). (b) SERS spectral features of <t>Au-nanoprobes</t> incubated with LAMP products of different concentrations of S. Enteritidis DNA template. SERS peak intensity centered at 583 cm -1 was used for quantitative analysis of S. Enteritidis. Variations in SERS peak intensity centered at 583 cm -1 were as a function of S. Enteritidis concentration. The inset shows a linear relationship in the concentration range from 6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL. Error bars are standard deviations from a total of three independent measurements. Raman spectroscopic analysis was performed using a 785-nm laser for excitation at 25 mW and 10 sec as integration time. The raw SERS spectra were baseline corrected and the final spectrum was obtained by averaging three different measurements without spectral normalization.
    Postamplification Labeling Step, supplied by Nanoprobes, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/postamplification labeling step/product/Nanoprobes
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    postamplification labeling step - by Bioz Stars, 2020-09
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    86
    Thermo Fisher postamplification melting curves
    Detection sensitivity of the developed LAMP-SERS assay. (a) Electrophoresis patterns of LAMP amplification products generated from different concentrations of S. Enteritidis DNA template. LAMP reaction was performed using 10-fold serial dilutions of S. Enteritidis DNA template (6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL). M: 1-kb DNA ladder marker; NC: negative control (without target DNA template). (b) SERS spectral features of <t>Au-nanoprobes</t> incubated with LAMP products of different concentrations of S. Enteritidis DNA template. SERS peak intensity centered at 583 cm -1 was used for quantitative analysis of S. Enteritidis. Variations in SERS peak intensity centered at 583 cm -1 were as a function of S. Enteritidis concentration. The inset shows a linear relationship in the concentration range from 6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL. Error bars are standard deviations from a total of three independent measurements. Raman spectroscopic analysis was performed using a 785-nm laser for excitation at 25 mW and 10 sec as integration time. The raw SERS spectra were baseline corrected and the final spectrum was obtained by averaging three different measurements without spectral normalization.
    Postamplification Melting Curves, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/postamplification melting curves/product/Thermo Fisher
    Average 86 stars, based on 10 article reviews
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    85
    Illumina Inc postamplification illumina libraries
    Detection sensitivity of the developed LAMP-SERS assay. (a) Electrophoresis patterns of LAMP amplification products generated from different concentrations of S. Enteritidis DNA template. LAMP reaction was performed using 10-fold serial dilutions of S. Enteritidis DNA template (6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL). M: 1-kb DNA ladder marker; NC: negative control (without target DNA template). (b) SERS spectral features of <t>Au-nanoprobes</t> incubated with LAMP products of different concentrations of S. Enteritidis DNA template. SERS peak intensity centered at 583 cm -1 was used for quantitative analysis of S. Enteritidis. Variations in SERS peak intensity centered at 583 cm -1 were as a function of S. Enteritidis concentration. The inset shows a linear relationship in the concentration range from 6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL. Error bars are standard deviations from a total of three independent measurements. Raman spectroscopic analysis was performed using a 785-nm laser for excitation at 25 mW and 10 sec as integration time. The raw SERS spectra were baseline corrected and the final spectrum was obtained by averaging three different measurements without spectral normalization.
    Postamplification Illumina Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/postamplification illumina libraries/product/Illumina Inc
    Average 85 stars, based on 3 article reviews
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    91
    Thermo Fisher samples post amplification
    Detection sensitivity of the developed LAMP-SERS assay. (a) Electrophoresis patterns of LAMP amplification products generated from different concentrations of S. Enteritidis DNA template. LAMP reaction was performed using 10-fold serial dilutions of S. Enteritidis DNA template (6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL). M: 1-kb DNA ladder marker; NC: negative control (without target DNA template). (b) SERS spectral features of <t>Au-nanoprobes</t> incubated with LAMP products of different concentrations of S. Enteritidis DNA template. SERS peak intensity centered at 583 cm -1 was used for quantitative analysis of S. Enteritidis. Variations in SERS peak intensity centered at 583 cm -1 were as a function of S. Enteritidis concentration. The inset shows a linear relationship in the concentration range from 6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL. Error bars are standard deviations from a total of three independent measurements. Raman spectroscopic analysis was performed using a 785-nm laser for excitation at 25 mW and 10 sec as integration time. The raw SERS spectra were baseline corrected and the final spectrum was obtained by averaging three different measurements without spectral normalization.
    Samples Post Amplification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/samples post amplification/product/Thermo Fisher
    Average 91 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    samples post amplification - by Bioz Stars, 2020-09
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    Image Search Results


    Detection sensitivity of the developed LAMP-SERS assay. (a) Electrophoresis patterns of LAMP amplification products generated from different concentrations of S. Enteritidis DNA template. LAMP reaction was performed using 10-fold serial dilutions of S. Enteritidis DNA template (6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL). M: 1-kb DNA ladder marker; NC: negative control (without target DNA template). (b) SERS spectral features of Au-nanoprobes incubated with LAMP products of different concentrations of S. Enteritidis DNA template. SERS peak intensity centered at 583 cm -1 was used for quantitative analysis of S. Enteritidis. Variations in SERS peak intensity centered at 583 cm -1 were as a function of S. Enteritidis concentration. The inset shows a linear relationship in the concentration range from 6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL. Error bars are standard deviations from a total of three independent measurements. Raman spectroscopic analysis was performed using a 785-nm laser for excitation at 25 mW and 10 sec as integration time. The raw SERS spectra were baseline corrected and the final spectrum was obtained by averaging three different measurements without spectral normalization.

    Journal: Theranostics

    Article Title: Development of a Loop Mediated Isothermal Amplification (LAMP) - Surface Enhanced Raman spectroscopy (SERS) Assay for the Detection of Salmonella Enterica Serotype Enteritidis

    doi: 10.7150/thno.14391

    Figure Lengend Snippet: Detection sensitivity of the developed LAMP-SERS assay. (a) Electrophoresis patterns of LAMP amplification products generated from different concentrations of S. Enteritidis DNA template. LAMP reaction was performed using 10-fold serial dilutions of S. Enteritidis DNA template (6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL). M: 1-kb DNA ladder marker; NC: negative control (without target DNA template). (b) SERS spectral features of Au-nanoprobes incubated with LAMP products of different concentrations of S. Enteritidis DNA template. SERS peak intensity centered at 583 cm -1 was used for quantitative analysis of S. Enteritidis. Variations in SERS peak intensity centered at 583 cm -1 were as a function of S. Enteritidis concentration. The inset shows a linear relationship in the concentration range from 6.6×10 0 CFU/mL - 6.6×10 6 CFU/mL. Error bars are standard deviations from a total of three independent measurements. Raman spectroscopic analysis was performed using a 785-nm laser for excitation at 25 mW and 10 sec as integration time. The raw SERS spectra were baseline corrected and the final spectrum was obtained by averaging three different measurements without spectral normalization.

    Article Snippet: This can be attributed to the combined action of Au-nanoprobes post-amplification labeling and nuclease digestion that allow differentiating the specific amplifications form the non-specific amplifications in the final step of SERS detection.

    Techniques: Electrophoresis, Amplification, Generated, Marker, Negative Control, Incubation, Concentration Assay, Size-exclusion Chromatography

    Preparation and characterization of Au-nanoprobes. (a) Characterization of gold nanoparticles: (i) TEM image; (ii) particle size distribution histogram. (b) Schematic illustration of the preparation of Au-nanoprobes. Citrate capped gold nanoparticles were modified with thiolated DNA oligonucleotides that is 20 mer in length and internally modified with cy5 (icy5). The image shows a slight change in color of gold nanoparticles solution caused by the conjugation of thiolated DNA to the surface of gold nanoparticles. However, no turbidity is visible, indicating that the stability of gold nanoparticles was maintained throughout the preparation reaction of Au-nanoprobes. (c) UV-vis absorption spectra of gold nanoparticles (citrate capped gold nanoparticles, black) and Au-nanoprobes (gold nanoparticles conjugated to DNA, red). (d) Raman spectra of pure cy5 modified DNA oligonucleotides (no gold nanoparticles, black) and Au-nanoprobes (cy5 modified DNA oligonucleotides conjugated gold nanoparticles, red), indicating a strong enhancement of cy5 Raman signal in the presence of gold nanoparticles.

    Journal: Theranostics

    Article Title: Development of a Loop Mediated Isothermal Amplification (LAMP) - Surface Enhanced Raman spectroscopy (SERS) Assay for the Detection of Salmonella Enterica Serotype Enteritidis

    doi: 10.7150/thno.14391

    Figure Lengend Snippet: Preparation and characterization of Au-nanoprobes. (a) Characterization of gold nanoparticles: (i) TEM image; (ii) particle size distribution histogram. (b) Schematic illustration of the preparation of Au-nanoprobes. Citrate capped gold nanoparticles were modified with thiolated DNA oligonucleotides that is 20 mer in length and internally modified with cy5 (icy5). The image shows a slight change in color of gold nanoparticles solution caused by the conjugation of thiolated DNA to the surface of gold nanoparticles. However, no turbidity is visible, indicating that the stability of gold nanoparticles was maintained throughout the preparation reaction of Au-nanoprobes. (c) UV-vis absorption spectra of gold nanoparticles (citrate capped gold nanoparticles, black) and Au-nanoprobes (gold nanoparticles conjugated to DNA, red). (d) Raman spectra of pure cy5 modified DNA oligonucleotides (no gold nanoparticles, black) and Au-nanoprobes (cy5 modified DNA oligonucleotides conjugated gold nanoparticles, red), indicating a strong enhancement of cy5 Raman signal in the presence of gold nanoparticles.

    Article Snippet: This can be attributed to the combined action of Au-nanoprobes post-amplification labeling and nuclease digestion that allow differentiating the specific amplifications form the non-specific amplifications in the final step of SERS detection.

    Techniques: Transmission Electron Microscopy, Modification, Conjugation Assay

    Nuclease digestion of the prepared Au-nanoprobes. (a) UV-vis absorption spectra of the centrifugation supernatants for the undigested (without the addition of nuclease, black) and the digested (after incubation with S1 nuclease, black) Au- nanoprobes. The presence of cy5 characteristic peak at 650 nm indicates the release of cy5 into the solution after digestion of Au-nanoprobe solution by S1 nuclease. (b) Fluorescence spectra of Au-nanoprobes before (black) and after (red) nuclease digestion, confirming a remarkable release of cy5 fluorescence after probe digestion by S1 nuclease. (c) Raman spectral features of the digested (black) and undigested (red) Au-nanoprobes. The corresponding schematic illustration shows the detailed structure of Au-nanoprobes and the products by S1 nuclease digestion. The digestion of ssDNA that is linked to the surface of gold nanoparticles by thiol-Au bond (red) results in the release of cy5 and free nucleotides (dNTs) into the reaction mixture. The release of cy5 from the surface of gold nanoparticles can be detected by measuring cy5 fluorescence and Raman signal. Increasing the distance between cy5 and gold nanoparticles results in the recovery of its fluorescence signal quenched by gold nanoparticles, but the effect of gold nanoparticles to enhance Raman signal intensity of cy5 is minimized. All spectra were obtained by averaging at least three independent measurements.

    Journal: Theranostics

    Article Title: Development of a Loop Mediated Isothermal Amplification (LAMP) - Surface Enhanced Raman spectroscopy (SERS) Assay for the Detection of Salmonella Enterica Serotype Enteritidis

    doi: 10.7150/thno.14391

    Figure Lengend Snippet: Nuclease digestion of the prepared Au-nanoprobes. (a) UV-vis absorption spectra of the centrifugation supernatants for the undigested (without the addition of nuclease, black) and the digested (after incubation with S1 nuclease, black) Au- nanoprobes. The presence of cy5 characteristic peak at 650 nm indicates the release of cy5 into the solution after digestion of Au-nanoprobe solution by S1 nuclease. (b) Fluorescence spectra of Au-nanoprobes before (black) and after (red) nuclease digestion, confirming a remarkable release of cy5 fluorescence after probe digestion by S1 nuclease. (c) Raman spectral features of the digested (black) and undigested (red) Au-nanoprobes. The corresponding schematic illustration shows the detailed structure of Au-nanoprobes and the products by S1 nuclease digestion. The digestion of ssDNA that is linked to the surface of gold nanoparticles by thiol-Au bond (red) results in the release of cy5 and free nucleotides (dNTs) into the reaction mixture. The release of cy5 from the surface of gold nanoparticles can be detected by measuring cy5 fluorescence and Raman signal. Increasing the distance between cy5 and gold nanoparticles results in the recovery of its fluorescence signal quenched by gold nanoparticles, but the effect of gold nanoparticles to enhance Raman signal intensity of cy5 is minimized. All spectra were obtained by averaging at least three independent measurements.

    Article Snippet: This can be attributed to the combined action of Au-nanoprobes post-amplification labeling and nuclease digestion that allow differentiating the specific amplifications form the non-specific amplifications in the final step of SERS detection.

    Techniques: Centrifugation, Incubation, Fluorescence

    Nuclease digestion of Au-nanoprobes in the presence of different concentrations of the target DNA. (a) Agarose gel electrophoresis of Au-nanoprobes (2 nM) after incubation with 50 µL of phosphate buffer (free of DNA) or with 1 µM of the synthetic target DNA (20-mer). (b) SERS detection of the target DNA (concentrations from 1-100 nM) using Au-nanoprobes (0.1 µM). Raman spectroscopic analysis was performed using a 785-nm laser for excitation at 25 mW and 10 sec as integration time. The raw SERS spectra were baseline corrected and the final spectrum was obtained by averaging three different measurements without spectral normalization.

    Journal: Theranostics

    Article Title: Development of a Loop Mediated Isothermal Amplification (LAMP) - Surface Enhanced Raman spectroscopy (SERS) Assay for the Detection of Salmonella Enterica Serotype Enteritidis

    doi: 10.7150/thno.14391

    Figure Lengend Snippet: Nuclease digestion of Au-nanoprobes in the presence of different concentrations of the target DNA. (a) Agarose gel electrophoresis of Au-nanoprobes (2 nM) after incubation with 50 µL of phosphate buffer (free of DNA) or with 1 µM of the synthetic target DNA (20-mer). (b) SERS detection of the target DNA (concentrations from 1-100 nM) using Au-nanoprobes (0.1 µM). Raman spectroscopic analysis was performed using a 785-nm laser for excitation at 25 mW and 10 sec as integration time. The raw SERS spectra were baseline corrected and the final spectrum was obtained by averaging three different measurements without spectral normalization.

    Article Snippet: This can be attributed to the combined action of Au-nanoprobes post-amplification labeling and nuclease digestion that allow differentiating the specific amplifications form the non-specific amplifications in the final step of SERS detection.

    Techniques: Agarose Gel Electrophoresis, Incubation, Size-exclusion Chromatography

    Detection specificity of the developed LAMP-SERS assay. (a) Electrophoresis patterns of LAMP amplification products generated from different bacteria. LAMP reaction was performed using DNA template extracted from different bacterial suspensions (OD 600 =1). Lane M: 1-kb DNA ladder marker; NC: negative control (without target DNA template); Lane1: S . Enteritidis (target bacterium); Lane 2: S . Typhimurium; Lane 3: S. aureus ; Lane 4: L . monocytogenes ; Lane 5: P. aeruginosa ; Lane 6: E . coli O157:H7. (b) SERS spectral features of Au-nanoprobes incubated with LAMP products of different bacteria. The inset shows the variation in SERS peak intensity centered at 583 cm -1 as a function of different bacteria. Error bars are standard deviations from a total of three independent measurements. Raman spectroscopic analysis was performed using a 785-nm laser for excitation at 25 mW and 10 sec as integration time. The raw SERS spectra were baseline corrected and the final spectrum was obtained by averaging three different measurements without spectral normalization.

    Journal: Theranostics

    Article Title: Development of a Loop Mediated Isothermal Amplification (LAMP) - Surface Enhanced Raman spectroscopy (SERS) Assay for the Detection of Salmonella Enterica Serotype Enteritidis

    doi: 10.7150/thno.14391

    Figure Lengend Snippet: Detection specificity of the developed LAMP-SERS assay. (a) Electrophoresis patterns of LAMP amplification products generated from different bacteria. LAMP reaction was performed using DNA template extracted from different bacterial suspensions (OD 600 =1). Lane M: 1-kb DNA ladder marker; NC: negative control (without target DNA template); Lane1: S . Enteritidis (target bacterium); Lane 2: S . Typhimurium; Lane 3: S. aureus ; Lane 4: L . monocytogenes ; Lane 5: P. aeruginosa ; Lane 6: E . coli O157:H7. (b) SERS spectral features of Au-nanoprobes incubated with LAMP products of different bacteria. The inset shows the variation in SERS peak intensity centered at 583 cm -1 as a function of different bacteria. Error bars are standard deviations from a total of three independent measurements. Raman spectroscopic analysis was performed using a 785-nm laser for excitation at 25 mW and 10 sec as integration time. The raw SERS spectra were baseline corrected and the final spectrum was obtained by averaging three different measurements without spectral normalization.

    Article Snippet: This can be attributed to the combined action of Au-nanoprobes post-amplification labeling and nuclease digestion that allow differentiating the specific amplifications form the non-specific amplifications in the final step of SERS detection.

    Techniques: Electrophoresis, Amplification, Generated, Marker, Negative Control, Incubation, Size-exclusion Chromatography

    Schematic illustration of LAMP-SERS assay. The developed LAMP-SERS assay comprises the following steps: 1) LAMP amplification of the target DNA using 4 particular in the presence of Bst enzyme and free dNTPs at 65 C for 40 min; 2) Addition of Au-nanoprobes that preferentially designed of 12-nm gold nanoparticles conjugated to thiolated 20-mer DNA oligonucleotides. In these probes, the surface DNA oligonucleotides act as capturing probes to specifically recognize the target DNA, meanwhile internally labeled with a Raman active dye of cy5 (at position 6 which is

    Journal: Theranostics

    Article Title: Development of a Loop Mediated Isothermal Amplification (LAMP) - Surface Enhanced Raman spectroscopy (SERS) Assay for the Detection of Salmonella Enterica Serotype Enteritidis

    doi: 10.7150/thno.14391

    Figure Lengend Snippet: Schematic illustration of LAMP-SERS assay. The developed LAMP-SERS assay comprises the following steps: 1) LAMP amplification of the target DNA using 4 particular in the presence of Bst enzyme and free dNTPs at 65 C for 40 min; 2) Addition of Au-nanoprobes that preferentially designed of 12-nm gold nanoparticles conjugated to thiolated 20-mer DNA oligonucleotides. In these probes, the surface DNA oligonucleotides act as capturing probes to specifically recognize the target DNA, meanwhile internally labeled with a Raman active dye of cy5 (at position 6 which is

    Article Snippet: This can be attributed to the combined action of Au-nanoprobes post-amplification labeling and nuclease digestion that allow differentiating the specific amplifications form the non-specific amplifications in the final step of SERS detection.

    Techniques: Amplification, Activated Clotting Time Assay, Labeling