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  • 88
    Bio-Rad polyvinylidinefluoride membranes
    Polyvinylidinefluoride Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore polyvinylidinefluoride membranes
    Polyvinylidinefluoride Membranes, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer polyvinylidenefluoride membranes
    Polyvinylidenefluoride Membranes, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATTO Corp polyvinylidenefluoride membranes
    Polyvinylidenefluoride Membranes, supplied by ATTO Corp, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polyvinylidenefluoride membrane
    Polyvinylidenefluoride Membrane, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polyvinylidenefluoride membranes
    Polyvinylidenefluoride Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polyvinylidenefluoride pvdf membranes
    Polyvinylidenefluoride Pvdf Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare polyvinylidenefluoride pvdf membrane
    Polyvinylidenefluoride Pvdf Membrane, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PerkinElmer polyvinylidenefluoride pvdf membranes
    Polyvinylidenefluoride Pvdf Membranes, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare hybond p polyvinylidenefluoride membrane
    Hybond P Polyvinylidenefluoride Membrane, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare polyvinylidenefluoride membrane
    Polyvinylidenefluoride Membrane, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore polyvinyldinefluoride membranes
    Polyvinyldinefluoride Membranes, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche polyvinylidenefluoride membrane
    Polyvinylidenefluoride Membrane, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA methanol activated polyvinylidenefluoride membranes
    Methanol Activated Polyvinylidenefluoride Membranes, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche methanol activated polyvinylidenefluoride membranes
    Western blot analysis of immunoreactive isotocin (Ist) receptor 1 (Istr1) and Istr2 in tissues of female (A–E) and male (F–J) ricefield eels. The tissue homogenates (500 µg) from the brain (Br), pituitary (Pi), gonad (Go), spleen (Sp), liver (Li), kindey (Ki), and intestine (In) were separated on 12% SDS-PAGE gels, transferred to <t>polyvinylidenefluoride</t> membranes, and then immunoreacted with the rabbit anti-Istr1 antiserum [1:1,000; (A,F) ], or rabbit anti-Istr1 antiserum pre-absorbed by excessive recombinant full-length Istr1 (B,G) , or the rabbit anti- Istr2 antiserum [1:1,000; (C,H) ], or rabbit anti-Istr2 antiserum pre-absorbed by excessive recombinant full-length Istr2 (D,I) , or mouse anti-Actb monoclonal antibody [1:2,000; catalog number: 60008-1-Ig; ProteinTech Group, Inc.; (E,J) ]. The secondary antibody was 1:5,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (H + L) (catalog number 115-035-003; Jackson ImmunoResearch Laboratories) or HRP-conjugated goat anti-rabbit IgG (H + L) (catalog number 111-035-003, Jackson ImmunoResearch Laboratories, Inc., PE, USA). The blots were visualized using the BeyoECL Plus kit (Beyotime).
    Methanol Activated Polyvinylidenefluoride Membranes, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bio-Rad immunoblot polyvinylidenefluoride membrane
    Western blot analysis of immunoreactive isotocin (Ist) receptor 1 (Istr1) and Istr2 in tissues of female (A–E) and male (F–J) ricefield eels. The tissue homogenates (500 µg) from the brain (Br), pituitary (Pi), gonad (Go), spleen (Sp), liver (Li), kindey (Ki), and intestine (In) were separated on 12% SDS-PAGE gels, transferred to <t>polyvinylidenefluoride</t> membranes, and then immunoreacted with the rabbit anti-Istr1 antiserum [1:1,000; (A,F) ], or rabbit anti-Istr1 antiserum pre-absorbed by excessive recombinant full-length Istr1 (B,G) , or the rabbit anti- Istr2 antiserum [1:1,000; (C,H) ], or rabbit anti-Istr2 antiserum pre-absorbed by excessive recombinant full-length Istr2 (D,I) , or mouse anti-Actb monoclonal antibody [1:2,000; catalog number: 60008-1-Ig; ProteinTech Group, Inc.; (E,J) ]. The secondary antibody was 1:5,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (H + L) (catalog number 115-035-003; Jackson ImmunoResearch Laboratories) or HRP-conjugated goat anti-rabbit IgG (H + L) (catalog number 111-035-003, Jackson ImmunoResearch Laboratories, Inc., PE, USA). The blots were visualized using the BeyoECL Plus kit (Beyotime).
    Immunoblot Polyvinylidenefluoride Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polyvinylidenefluoride biorad membranes
    Western blot analysis of immunoreactive isotocin (Ist) receptor 1 (Istr1) and Istr2 in tissues of female (A–E) and male (F–J) ricefield eels. The tissue homogenates (500 µg) from the brain (Br), pituitary (Pi), gonad (Go), spleen (Sp), liver (Li), kindey (Ki), and intestine (In) were separated on 12% SDS-PAGE gels, transferred to <t>polyvinylidenefluoride</t> membranes, and then immunoreacted with the rabbit anti-Istr1 antiserum [1:1,000; (A,F) ], or rabbit anti-Istr1 antiserum pre-absorbed by excessive recombinant full-length Istr1 (B,G) , or the rabbit anti- Istr2 antiserum [1:1,000; (C,H) ], or rabbit anti-Istr2 antiserum pre-absorbed by excessive recombinant full-length Istr2 (D,I) , or mouse anti-Actb monoclonal antibody [1:2,000; catalog number: 60008-1-Ig; ProteinTech Group, Inc.; (E,J) ]. The secondary antibody was 1:5,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (H + L) (catalog number 115-035-003; Jackson ImmunoResearch Laboratories) or HRP-conjugated goat anti-rabbit IgG (H + L) (catalog number 111-035-003, Jackson ImmunoResearch Laboratories, Inc., PE, USA). The blots were visualized using the BeyoECL Plus kit (Beyotime).
    Polyvinylidenefluoride Biorad Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad polyvinylidenefluoride pdvf membranes
    Western blot analysis of immunoreactive isotocin (Ist) receptor 1 (Istr1) and Istr2 in tissues of female (A–E) and male (F–J) ricefield eels. The tissue homogenates (500 µg) from the brain (Br), pituitary (Pi), gonad (Go), spleen (Sp), liver (Li), kindey (Ki), and intestine (In) were separated on 12% SDS-PAGE gels, transferred to <t>polyvinylidenefluoride</t> membranes, and then immunoreacted with the rabbit anti-Istr1 antiserum [1:1,000; (A,F) ], or rabbit anti-Istr1 antiserum pre-absorbed by excessive recombinant full-length Istr1 (B,G) , or the rabbit anti- Istr2 antiserum [1:1,000; (C,H) ], or rabbit anti-Istr2 antiserum pre-absorbed by excessive recombinant full-length Istr2 (D,I) , or mouse anti-Actb monoclonal antibody [1:2,000; catalog number: 60008-1-Ig; ProteinTech Group, Inc.; (E,J) ]. The secondary antibody was 1:5,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (H + L) (catalog number 115-035-003; Jackson ImmunoResearch Laboratories) or HRP-conjugated goat anti-rabbit IgG (H + L) (catalog number 111-035-003, Jackson ImmunoResearch Laboratories, Inc., PE, USA). The blots were visualized using the BeyoECL Plus kit (Beyotime).
    Polyvinylidenefluoride Pdvf Membranes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare polyvinylidenefluoride pvdf hybond p membranes
    Western blot analysis of immunoreactive isotocin (Ist) receptor 1 (Istr1) and Istr2 in tissues of female (A–E) and male (F–J) ricefield eels. The tissue homogenates (500 µg) from the brain (Br), pituitary (Pi), gonad (Go), spleen (Sp), liver (Li), kindey (Ki), and intestine (In) were separated on 12% SDS-PAGE gels, transferred to <t>polyvinylidenefluoride</t> membranes, and then immunoreacted with the rabbit anti-Istr1 antiserum [1:1,000; (A,F) ], or rabbit anti-Istr1 antiserum pre-absorbed by excessive recombinant full-length Istr1 (B,G) , or the rabbit anti- Istr2 antiserum [1:1,000; (C,H) ], or rabbit anti-Istr2 antiserum pre-absorbed by excessive recombinant full-length Istr2 (D,I) , or mouse anti-Actb monoclonal antibody [1:2,000; catalog number: 60008-1-Ig; ProteinTech Group, Inc.; (E,J) ]. The secondary antibody was 1:5,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (H + L) (catalog number 115-035-003; Jackson ImmunoResearch Laboratories) or HRP-conjugated goat anti-rabbit IgG (H + L) (catalog number 111-035-003, Jackson ImmunoResearch Laboratories, Inc., PE, USA). The blots were visualized using the BeyoECL Plus kit (Beyotime).
    Polyvinylidenefluoride Pvdf Hybond P Membranes, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Roche polyvinylidenefluoride pvdf membrane
    Western blot analysis of immunoreactive isotocin (Ist) receptor 1 (Istr1) and Istr2 in tissues of female (A–E) and male (F–J) ricefield eels. The tissue homogenates (500 µg) from the brain (Br), pituitary (Pi), gonad (Go), spleen (Sp), liver (Li), kindey (Ki), and intestine (In) were separated on 12% SDS-PAGE gels, transferred to <t>polyvinylidenefluoride</t> membranes, and then immunoreacted with the rabbit anti-Istr1 antiserum [1:1,000; (A,F) ], or rabbit anti-Istr1 antiserum pre-absorbed by excessive recombinant full-length Istr1 (B,G) , or the rabbit anti- Istr2 antiserum [1:1,000; (C,H) ], or rabbit anti-Istr2 antiserum pre-absorbed by excessive recombinant full-length Istr2 (D,I) , or mouse anti-Actb monoclonal antibody [1:2,000; catalog number: 60008-1-Ig; ProteinTech Group, Inc.; (E,J) ]. The secondary antibody was 1:5,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (H + L) (catalog number 115-035-003; Jackson ImmunoResearch Laboratories) or HRP-conjugated goat anti-rabbit IgG (H + L) (catalog number 111-035-003, Jackson ImmunoResearch Laboratories, Inc., PE, USA). The blots were visualized using the BeyoECL Plus kit (Beyotime).
    Polyvinylidenefluoride Pvdf Membrane, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher polyvinylidenefluoride pvdf membrane
    Western blot analysis of immunoreactive isotocin (Ist) receptor 1 (Istr1) and Istr2 in tissues of female (A–E) and male (F–J) ricefield eels. The tissue homogenates (500 µg) from the brain (Br), pituitary (Pi), gonad (Go), spleen (Sp), liver (Li), kindey (Ki), and intestine (In) were separated on 12% SDS-PAGE gels, transferred to <t>polyvinylidenefluoride</t> membranes, and then immunoreacted with the rabbit anti-Istr1 antiserum [1:1,000; (A,F) ], or rabbit anti-Istr1 antiserum pre-absorbed by excessive recombinant full-length Istr1 (B,G) , or the rabbit anti- Istr2 antiserum [1:1,000; (C,H) ], or rabbit anti-Istr2 antiserum pre-absorbed by excessive recombinant full-length Istr2 (D,I) , or mouse anti-Actb monoclonal antibody [1:2,000; catalog number: 60008-1-Ig; ProteinTech Group, Inc.; (E,J) ]. The secondary antibody was 1:5,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (H + L) (catalog number 115-035-003; Jackson ImmunoResearch Laboratories) or HRP-conjugated goat anti-rabbit IgG (H + L) (catalog number 111-035-003, Jackson ImmunoResearch Laboratories, Inc., PE, USA). The blots were visualized using the BeyoECL Plus kit (Beyotime).
    Polyvinylidenefluoride Pvdf Membrane, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Carl Roth GmbH polyvinylidenefluoride pvdf membrane
    Western blot analysis of immunoreactive isotocin (Ist) receptor 1 (Istr1) and Istr2 in tissues of female (A–E) and male (F–J) ricefield eels. The tissue homogenates (500 µg) from the brain (Br), pituitary (Pi), gonad (Go), spleen (Sp), liver (Li), kindey (Ki), and intestine (In) were separated on 12% SDS-PAGE gels, transferred to <t>polyvinylidenefluoride</t> membranes, and then immunoreacted with the rabbit anti-Istr1 antiserum [1:1,000; (A,F) ], or rabbit anti-Istr1 antiserum pre-absorbed by excessive recombinant full-length Istr1 (B,G) , or the rabbit anti- Istr2 antiserum [1:1,000; (C,H) ], or rabbit anti-Istr2 antiserum pre-absorbed by excessive recombinant full-length Istr2 (D,I) , or mouse anti-Actb monoclonal antibody [1:2,000; catalog number: 60008-1-Ig; ProteinTech Group, Inc.; (E,J) ]. The secondary antibody was 1:5,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (H + L) (catalog number 115-035-003; Jackson ImmunoResearch Laboratories) or HRP-conjugated goat anti-rabbit IgG (H + L) (catalog number 111-035-003, Jackson ImmunoResearch Laboratories, Inc., PE, USA). The blots were visualized using the BeyoECL Plus kit (Beyotime).
    Polyvinylidenefluoride Pvdf Membrane, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare hybond p membrane
    Western blot analysis of immunoreactive isotocin (Ist) receptor 1 (Istr1) and Istr2 in tissues of female (A–E) and male (F–J) ricefield eels. The tissue homogenates (500 µg) from the brain (Br), pituitary (Pi), gonad (Go), spleen (Sp), liver (Li), kindey (Ki), and intestine (In) were separated on 12% SDS-PAGE gels, transferred to <t>polyvinylidenefluoride</t> membranes, and then immunoreacted with the rabbit anti-Istr1 antiserum [1:1,000; (A,F) ], or rabbit anti-Istr1 antiserum pre-absorbed by excessive recombinant full-length Istr1 (B,G) , or the rabbit anti- Istr2 antiserum [1:1,000; (C,H) ], or rabbit anti-Istr2 antiserum pre-absorbed by excessive recombinant full-length Istr2 (D,I) , or mouse anti-Actb monoclonal antibody [1:2,000; catalog number: 60008-1-Ig; ProteinTech Group, Inc.; (E,J) ]. The secondary antibody was 1:5,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (H + L) (catalog number 115-035-003; Jackson ImmunoResearch Laboratories) or HRP-conjugated goat anti-rabbit IgG (H + L) (catalog number 111-035-003, Jackson ImmunoResearch Laboratories, Inc., PE, USA). The blots were visualized using the BeyoECL Plus kit (Beyotime).
    Hybond P Membrane, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 2096 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Western blot analysis of immunoreactive isotocin (Ist) receptor 1 (Istr1) and Istr2 in tissues of female (A–E) and male (F–J) ricefield eels. The tissue homogenates (500 µg) from the brain (Br), pituitary (Pi), gonad (Go), spleen (Sp), liver (Li), kindey (Ki), and intestine (In) were separated on 12% SDS-PAGE gels, transferred to polyvinylidenefluoride membranes, and then immunoreacted with the rabbit anti-Istr1 antiserum [1:1,000; (A,F) ], or rabbit anti-Istr1 antiserum pre-absorbed by excessive recombinant full-length Istr1 (B,G) , or the rabbit anti- Istr2 antiserum [1:1,000; (C,H) ], or rabbit anti-Istr2 antiserum pre-absorbed by excessive recombinant full-length Istr2 (D,I) , or mouse anti-Actb monoclonal antibody [1:2,000; catalog number: 60008-1-Ig; ProteinTech Group, Inc.; (E,J) ]. The secondary antibody was 1:5,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (H + L) (catalog number 115-035-003; Jackson ImmunoResearch Laboratories) or HRP-conjugated goat anti-rabbit IgG (H + L) (catalog number 111-035-003, Jackson ImmunoResearch Laboratories, Inc., PE, USA). The blots were visualized using the BeyoECL Plus kit (Beyotime).

    Journal: Frontiers in Endocrinology

    Article Title: Isotocin Regulates Growth Hormone but Not Prolactin Release From the Pituitary of Ricefield Eels

    doi: 10.3389/fendo.2018.00166

    Figure Lengend Snippet: Western blot analysis of immunoreactive isotocin (Ist) receptor 1 (Istr1) and Istr2 in tissues of female (A–E) and male (F–J) ricefield eels. The tissue homogenates (500 µg) from the brain (Br), pituitary (Pi), gonad (Go), spleen (Sp), liver (Li), kindey (Ki), and intestine (In) were separated on 12% SDS-PAGE gels, transferred to polyvinylidenefluoride membranes, and then immunoreacted with the rabbit anti-Istr1 antiserum [1:1,000; (A,F) ], or rabbit anti-Istr1 antiserum pre-absorbed by excessive recombinant full-length Istr1 (B,G) , or the rabbit anti- Istr2 antiserum [1:1,000; (C,H) ], or rabbit anti-Istr2 antiserum pre-absorbed by excessive recombinant full-length Istr2 (D,I) , or mouse anti-Actb monoclonal antibody [1:2,000; catalog number: 60008-1-Ig; ProteinTech Group, Inc.; (E,J) ]. The secondary antibody was 1:5,000 diluted horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG) (H + L) (catalog number 115-035-003; Jackson ImmunoResearch Laboratories) or HRP-conjugated goat anti-rabbit IgG (H + L) (catalog number 111-035-003, Jackson ImmunoResearch Laboratories, Inc., PE, USA). The blots were visualized using the BeyoECL Plus kit (Beyotime).

    Article Snippet: Western Blot Analysis The recombinant proteins or tissue homogenates of the brain, pituitary, gonad, spleen, liver, kidney, and intestine from female or male ricefield eels were separated on 12% SDS-PAGE gels and transferred to methanol-activated polyvinylidenefluoride membranes (Roche, Mannheim, Germany) by electroblotting.

    Techniques: Western Blot, SDS Page, Recombinant