polypropylene tubes Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Thermo Fisher polypropylene tubes
    Polypropylene Tubes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene tubes/product/Thermo Fisher
    Average 96 stars, based on 211 article reviews
    Price from $9.99 to $1999.99
    polypropylene tubes - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    99
    Millipore polypropylene tubes
    Polypropylene Tubes, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 384 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene tubes/product/Millipore
    Average 99 stars, based on 384 article reviews
    Price from $9.99 to $1999.99
    polypropylene tubes - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Becton Dickinson polypropylene tubes
    Polypropylene Tubes, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 1056 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene tubes/product/Becton Dickinson
    Average 92 stars, based on 1056 article reviews
    Price from $9.99 to $1999.99
    polypropylene tubes - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    93
    Sarstedt polypropylene tubes
    Polypropylene Tubes, supplied by Sarstedt, used in various techniques. Bioz Stars score: 93/100, based on 361 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene tubes/product/Sarstedt
    Average 93 stars, based on 361 article reviews
    Price from $9.99 to $1999.99
    polypropylene tubes - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    91
    Thermo Fisher nunc conical sterile polypropylene centrifuge tubes
    Nunc Conical Sterile Polypropylene Centrifuge Tubes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nunc conical sterile polypropylene centrifuge tubes/product/Thermo Fisher
    Average 91 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    nunc conical sterile polypropylene centrifuge tubes - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    99
    Eppendorf AG polypropylene eppendorf tubes
    Monocyte and endothelial cell binding of MN10021 and MN20054. A. Human blood monocytes, isolated as described in Methods, were adjusted to 5×10 6 /ml in PBS (0.5% BSA, pH 7.5). Two-tenths ml of cell suspension was added to each of triplicate 12×75 mm polypropylene tubes and the tubes were incubated for 60 min at 37°C with 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The contents of each tube were then layered onto 0.8 ml of 10% sucrose in <t>Eppendorf</t> tubes, centrifuged, and the cell-associated radioactivity determined by gamma scintillation spectrophotometry of the cell pellet. B. Binding/uptake of [ 125 I]-MN20054 by human monocytes was performed as described for panel A with the following exceptions: (1) half the cells were pretreated for 30 min at 37°C with cytochalasin B to inhibit endocytosis and (2) competition was performed only with a 100-fold molar excess of MN10021. C. HUVEC were grown to confluence in 12-well tissue culture plates. To each of triplicate wells was added 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The plates were incubated for 60 min at 37°C with gentle rocking, the wells aspirated and quickly washed 3X with 2.0 ml each of ice-cold PBS/BSA and 1.0 ml of 1.0 N NaOH added to each well to solubilize the cells. Nine-tenths ml of solubilized cells was then removed from each well and cell-associated radioactivity determined by gamma scintillation spectrophotometry.
    Polypropylene Eppendorf Tubes, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene eppendorf tubes/product/Eppendorf AG
    Average 99 stars, based on 188 article reviews
    Price from $9.99 to $1999.99
    polypropylene eppendorf tubes - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    93
    Greiner Bio polypropylene tubes
    Monocyte and endothelial cell binding of MN10021 and MN20054. A. Human blood monocytes, isolated as described in Methods, were adjusted to 5×10 6 /ml in PBS (0.5% BSA, pH 7.5). Two-tenths ml of cell suspension was added to each of triplicate 12×75 mm polypropylene tubes and the tubes were incubated for 60 min at 37°C with 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The contents of each tube were then layered onto 0.8 ml of 10% sucrose in <t>Eppendorf</t> tubes, centrifuged, and the cell-associated radioactivity determined by gamma scintillation spectrophotometry of the cell pellet. B. Binding/uptake of [ 125 I]-MN20054 by human monocytes was performed as described for panel A with the following exceptions: (1) half the cells were pretreated for 30 min at 37°C with cytochalasin B to inhibit endocytosis and (2) competition was performed only with a 100-fold molar excess of MN10021. C. HUVEC were grown to confluence in 12-well tissue culture plates. To each of triplicate wells was added 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The plates were incubated for 60 min at 37°C with gentle rocking, the wells aspirated and quickly washed 3X with 2.0 ml each of ice-cold PBS/BSA and 1.0 ml of 1.0 N NaOH added to each well to solubilize the cells. Nine-tenths ml of solubilized cells was then removed from each well and cell-associated radioactivity determined by gamma scintillation spectrophotometry.
    Polypropylene Tubes, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 93/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene tubes/product/Greiner Bio
    Average 93 stars, based on 126 article reviews
    Price from $9.99 to $1999.99
    polypropylene tubes - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    93
    Corning Life Sciences polypropylene tubes
    Monocyte and endothelial cell binding of MN10021 and MN20054. A. Human blood monocytes, isolated as described in Methods, were adjusted to 5×10 6 /ml in PBS (0.5% BSA, pH 7.5). Two-tenths ml of cell suspension was added to each of triplicate 12×75 mm polypropylene tubes and the tubes were incubated for 60 min at 37°C with 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The contents of each tube were then layered onto 0.8 ml of 10% sucrose in <t>Eppendorf</t> tubes, centrifuged, and the cell-associated radioactivity determined by gamma scintillation spectrophotometry of the cell pellet. B. Binding/uptake of [ 125 I]-MN20054 by human monocytes was performed as described for panel A with the following exceptions: (1) half the cells were pretreated for 30 min at 37°C with cytochalasin B to inhibit endocytosis and (2) competition was performed only with a 100-fold molar excess of MN10021. C. HUVEC were grown to confluence in 12-well tissue culture plates. To each of triplicate wells was added 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The plates were incubated for 60 min at 37°C with gentle rocking, the wells aspirated and quickly washed 3X with 2.0 ml each of ice-cold PBS/BSA and 1.0 ml of 1.0 N NaOH added to each well to solubilize the cells. Nine-tenths ml of solubilized cells was then removed from each well and cell-associated radioactivity determined by gamma scintillation spectrophotometry.
    Polypropylene Tubes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene tubes/product/Corning Life Sciences
    Average 93 stars, based on 115 article reviews
    Price from $9.99 to $1999.99
    polypropylene tubes - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    90
    Becton Dickinson polypropylene conical tubes
    Monocyte and endothelial cell binding of MN10021 and MN20054. A. Human blood monocytes, isolated as described in Methods, were adjusted to 5×10 6 /ml in PBS (0.5% BSA, pH 7.5). Two-tenths ml of cell suspension was added to each of triplicate 12×75 mm polypropylene tubes and the tubes were incubated for 60 min at 37°C with 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The contents of each tube were then layered onto 0.8 ml of 10% sucrose in <t>Eppendorf</t> tubes, centrifuged, and the cell-associated radioactivity determined by gamma scintillation spectrophotometry of the cell pellet. B. Binding/uptake of [ 125 I]-MN20054 by human monocytes was performed as described for panel A with the following exceptions: (1) half the cells were pretreated for 30 min at 37°C with cytochalasin B to inhibit endocytosis and (2) competition was performed only with a 100-fold molar excess of MN10021. C. HUVEC were grown to confluence in 12-well tissue culture plates. To each of triplicate wells was added 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The plates were incubated for 60 min at 37°C with gentle rocking, the wells aspirated and quickly washed 3X with 2.0 ml each of ice-cold PBS/BSA and 1.0 ml of 1.0 N NaOH added to each well to solubilize the cells. Nine-tenths ml of solubilized cells was then removed from each well and cell-associated radioactivity determined by gamma scintillation spectrophotometry.
    Polypropylene Conical Tubes, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene conical tubes/product/Becton Dickinson
    Average 90 stars, based on 151 article reviews
    Price from $9.99 to $1999.99
    polypropylene conical tubes - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    90
    Corning Life Sciences polypropylene centrifuge tubes
    Monocyte and endothelial cell binding of MN10021 and MN20054. A. Human blood monocytes, isolated as described in Methods, were adjusted to 5×10 6 /ml in PBS (0.5% BSA, pH 7.5). Two-tenths ml of cell suspension was added to each of triplicate 12×75 mm polypropylene tubes and the tubes were incubated for 60 min at 37°C with 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The contents of each tube were then layered onto 0.8 ml of 10% sucrose in <t>Eppendorf</t> tubes, centrifuged, and the cell-associated radioactivity determined by gamma scintillation spectrophotometry of the cell pellet. B. Binding/uptake of [ 125 I]-MN20054 by human monocytes was performed as described for panel A with the following exceptions: (1) half the cells were pretreated for 30 min at 37°C with cytochalasin B to inhibit endocytosis and (2) competition was performed only with a 100-fold molar excess of MN10021. C. HUVEC were grown to confluence in 12-well tissue culture plates. To each of triplicate wells was added 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The plates were incubated for 60 min at 37°C with gentle rocking, the wells aspirated and quickly washed 3X with 2.0 ml each of ice-cold PBS/BSA and 1.0 ml of 1.0 N NaOH added to each well to solubilize the cells. Nine-tenths ml of solubilized cells was then removed from each well and cell-associated radioactivity determined by gamma scintillation spectrophotometry.
    Polypropylene Centrifuge Tubes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene centrifuge tubes/product/Corning Life Sciences
    Average 90 stars, based on 83 article reviews
    Price from $9.99 to $1999.99
    polypropylene centrifuge tubes - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    92
    Fisher Scientific polypropylene tubes
    Monocyte and endothelial cell binding of MN10021 and MN20054. A. Human blood monocytes, isolated as described in Methods, were adjusted to 5×10 6 /ml in PBS (0.5% BSA, pH 7.5). Two-tenths ml of cell suspension was added to each of triplicate 12×75 mm polypropylene tubes and the tubes were incubated for 60 min at 37°C with 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The contents of each tube were then layered onto 0.8 ml of 10% sucrose in <t>Eppendorf</t> tubes, centrifuged, and the cell-associated radioactivity determined by gamma scintillation spectrophotometry of the cell pellet. B. Binding/uptake of [ 125 I]-MN20054 by human monocytes was performed as described for panel A with the following exceptions: (1) half the cells were pretreated for 30 min at 37°C with cytochalasin B to inhibit endocytosis and (2) competition was performed only with a 100-fold molar excess of MN10021. C. HUVEC were grown to confluence in 12-well tissue culture plates. To each of triplicate wells was added 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The plates were incubated for 60 min at 37°C with gentle rocking, the wells aspirated and quickly washed 3X with 2.0 ml each of ice-cold PBS/BSA and 1.0 ml of 1.0 N NaOH added to each well to solubilize the cells. Nine-tenths ml of solubilized cells was then removed from each well and cell-associated radioactivity determined by gamma scintillation spectrophotometry.
    Polypropylene Tubes, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene tubes/product/Fisher Scientific
    Average 92 stars, based on 111 article reviews
    Price from $9.99 to $1999.99
    polypropylene tubes - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    Millipore polypropylene tube
    Monocyte and endothelial cell binding of MN10021 and MN20054. A. Human blood monocytes, isolated as described in Methods, were adjusted to 5×10 6 /ml in PBS (0.5% BSA, pH 7.5). Two-tenths ml of cell suspension was added to each of triplicate 12×75 mm polypropylene tubes and the tubes were incubated for 60 min at 37°C with 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The contents of each tube were then layered onto 0.8 ml of 10% sucrose in <t>Eppendorf</t> tubes, centrifuged, and the cell-associated radioactivity determined by gamma scintillation spectrophotometry of the cell pellet. B. Binding/uptake of [ 125 I]-MN20054 by human monocytes was performed as described for panel A with the following exceptions: (1) half the cells were pretreated for 30 min at 37°C with cytochalasin B to inhibit endocytosis and (2) competition was performed only with a 100-fold molar excess of MN10021. C. HUVEC were grown to confluence in 12-well tissue culture plates. To each of triplicate wells was added 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The plates were incubated for 60 min at 37°C with gentle rocking, the wells aspirated and quickly washed 3X with 2.0 ml each of ice-cold PBS/BSA and 1.0 ml of 1.0 N NaOH added to each well to solubilize the cells. Nine-tenths ml of solubilized cells was then removed from each well and cell-associated radioactivity determined by gamma scintillation spectrophotometry.
    Polypropylene Tube, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 163 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene tube/product/Millipore
    Average 99 stars, based on 163 article reviews
    Price from $9.99 to $1999.99
    polypropylene tube - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    90
    Fisher Scientific polypropylene microcentrifuge tubes
    Monocyte and endothelial cell binding of MN10021 and MN20054. A. Human blood monocytes, isolated as described in Methods, were adjusted to 5×10 6 /ml in PBS (0.5% BSA, pH 7.5). Two-tenths ml of cell suspension was added to each of triplicate 12×75 mm polypropylene tubes and the tubes were incubated for 60 min at 37°C with 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The contents of each tube were then layered onto 0.8 ml of 10% sucrose in <t>Eppendorf</t> tubes, centrifuged, and the cell-associated radioactivity determined by gamma scintillation spectrophotometry of the cell pellet. B. Binding/uptake of [ 125 I]-MN20054 by human monocytes was performed as described for panel A with the following exceptions: (1) half the cells were pretreated for 30 min at 37°C with cytochalasin B to inhibit endocytosis and (2) competition was performed only with a 100-fold molar excess of MN10021. C. HUVEC were grown to confluence in 12-well tissue culture plates. To each of triplicate wells was added 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The plates were incubated for 60 min at 37°C with gentle rocking, the wells aspirated and quickly washed 3X with 2.0 ml each of ice-cold PBS/BSA and 1.0 ml of 1.0 N NaOH added to each well to solubilize the cells. Nine-tenths ml of solubilized cells was then removed from each well and cell-associated radioactivity determined by gamma scintillation spectrophotometry.
    Polypropylene Microcentrifuge Tubes, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene microcentrifuge tubes/product/Fisher Scientific
    Average 90 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    polypropylene microcentrifuge tubes - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    93
    Avantor polypropylene tubes
    Monocyte and endothelial cell binding of MN10021 and MN20054. A. Human blood monocytes, isolated as described in Methods, were adjusted to 5×10 6 /ml in PBS (0.5% BSA, pH 7.5). Two-tenths ml of cell suspension was added to each of triplicate 12×75 mm polypropylene tubes and the tubes were incubated for 60 min at 37°C with 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The contents of each tube were then layered onto 0.8 ml of 10% sucrose in <t>Eppendorf</t> tubes, centrifuged, and the cell-associated radioactivity determined by gamma scintillation spectrophotometry of the cell pellet. B. Binding/uptake of [ 125 I]-MN20054 by human monocytes was performed as described for panel A with the following exceptions: (1) half the cells were pretreated for 30 min at 37°C with cytochalasin B to inhibit endocytosis and (2) competition was performed only with a 100-fold molar excess of MN10021. C. HUVEC were grown to confluence in 12-well tissue culture plates. To each of triplicate wells was added 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The plates were incubated for 60 min at 37°C with gentle rocking, the wells aspirated and quickly washed 3X with 2.0 ml each of ice-cold PBS/BSA and 1.0 ml of 1.0 N NaOH added to each well to solubilize the cells. Nine-tenths ml of solubilized cells was then removed from each well and cell-associated radioactivity determined by gamma scintillation spectrophotometry.
    Polypropylene Tubes, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polypropylene tubes/product/Avantor
    Average 93 stars, based on 72 article reviews
    Price from $9.99 to $1999.99
    polypropylene tubes - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Monocyte and endothelial cell binding of MN10021 and MN20054. A. Human blood monocytes, isolated as described in Methods, were adjusted to 5×10 6 /ml in PBS (0.5% BSA, pH 7.5). Two-tenths ml of cell suspension was added to each of triplicate 12×75 mm polypropylene tubes and the tubes were incubated for 60 min at 37°C with 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The contents of each tube were then layered onto 0.8 ml of 10% sucrose in Eppendorf tubes, centrifuged, and the cell-associated radioactivity determined by gamma scintillation spectrophotometry of the cell pellet. B. Binding/uptake of [ 125 I]-MN20054 by human monocytes was performed as described for panel A with the following exceptions: (1) half the cells were pretreated for 30 min at 37°C with cytochalasin B to inhibit endocytosis and (2) competition was performed only with a 100-fold molar excess of MN10021. C. HUVEC were grown to confluence in 12-well tissue culture plates. To each of triplicate wells was added 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The plates were incubated for 60 min at 37°C with gentle rocking, the wells aspirated and quickly washed 3X with 2.0 ml each of ice-cold PBS/BSA and 1.0 ml of 1.0 N NaOH added to each well to solubilize the cells. Nine-tenths ml of solubilized cells was then removed from each well and cell-associated radioactivity determined by gamma scintillation spectrophotometry.

    Journal: PLoS ONE

    Article Title: Anti-Inflammatory and Vasoprotective Activity of a Retroviral-Derived Peptide, Homologous to Human Endogenous Retroviruses: Endothelial Cell Effects

    doi: 10.1371/journal.pone.0052693

    Figure Lengend Snippet: Monocyte and endothelial cell binding of MN10021 and MN20054. A. Human blood monocytes, isolated as described in Methods, were adjusted to 5×10 6 /ml in PBS (0.5% BSA, pH 7.5). Two-tenths ml of cell suspension was added to each of triplicate 12×75 mm polypropylene tubes and the tubes were incubated for 60 min at 37°C with 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The contents of each tube were then layered onto 0.8 ml of 10% sucrose in Eppendorf tubes, centrifuged, and the cell-associated radioactivity determined by gamma scintillation spectrophotometry of the cell pellet. B. Binding/uptake of [ 125 I]-MN20054 by human monocytes was performed as described for panel A with the following exceptions: (1) half the cells were pretreated for 30 min at 37°C with cytochalasin B to inhibit endocytosis and (2) competition was performed only with a 100-fold molar excess of MN10021. C. HUVEC were grown to confluence in 12-well tissue culture plates. To each of triplicate wells was added 20 pmol of [ 125 I]-MN20054 in the presence of either buffer alone, a 100-fold molar excess of MN20054, a 100-fold molar excess of MN10021, or a 100-fold molar excess of MN20050. The plates were incubated for 60 min at 37°C with gentle rocking, the wells aspirated and quickly washed 3X with 2.0 ml each of ice-cold PBS/BSA and 1.0 ml of 1.0 N NaOH added to each well to solubilize the cells. Nine-tenths ml of solubilized cells was then removed from each well and cell-associated radioactivity determined by gamma scintillation spectrophotometry.

    Article Snippet: The contents of each tube were layered onto a layer of 0.8 ml of 10% sucrose in polypropylene Eppendorf tubes and the tubes centrifuged for 5 min at 8,000×g to separate cell-associated from free ligand.

    Techniques: Binding Assay, Isolation, Incubation, Radioactivity, Spectrophotometry

    Doxorubicin remaining in solution in polypropylene microcentrifuge tubes at 37°C over 48 h at pH 4.8 a , pH 6.5 b , and pH 7.4 c . Each value represents the mean ± SD ( n = 4).

    Journal: AAPS PharmSciTech

    Article Title: Adsorption and Degradation of Doxorubicin from Aqueous Solution in Polypropylene Containers

    doi: 10.1208/s12249-012-9885-1

    Figure Lengend Snippet: Doxorubicin remaining in solution in polypropylene microcentrifuge tubes at 37°C over 48 h at pH 4.8 a , pH 6.5 b , and pH 7.4 c . Each value represents the mean ± SD ( n = 4).

    Article Snippet: The amounts of doxorubicin remaining in solution from 3 to 48 h at 37°C in polypropylene microcentrifuge tubes are shown in Fig. , , and at pH values of 4.8, 6.5, and 7.4, respectively, for various starting concentrations.

    Techniques: