polynucleotide kinase buffer Thermo Fisher Search Results


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  • 90
    Thermo Fisher polynucleotide kinase buffer
    Polynucleotide Kinase Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polynucleotide kinase pnk buffer
    Polynucleotide Kinase Pnk Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher t4 pnk
    T4 Pnk, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 812 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher polynucleotide kinase pnk buffer a buffer a 50 mm tris hcl
    Polynucleotide Kinase Pnk Buffer A Buffer A 50 Mm Tris Hcl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher t4 polynucleotide kinase pnk reaction buffer a
    T4 Polynucleotide Kinase Pnk Reaction Buffer A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher mapk lysis buffer
    Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in <t>Brij58</t> containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.
    Mapk Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 923 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher buffer a
    Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in <t>Brij58</t> containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.
    Buffer A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5830 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 18427 013 klenow dna polymerase t4 pnk minelute reaction cleanup kit
    Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in <t>Brij58</t> containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.
    18427 013 Klenow Dna Polymerase T4 Pnk Minelute Reaction Cleanup Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher lysis buffer
    Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in <t>Brij58</t> containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.
    Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 41889 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher novex tbe running buffer
    Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in <t>Brij58</t> containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.
    Novex Tbe Running Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sodium acetate buffer
    Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in <t>Brij58</t> containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.
    Sodium Acetate Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher chek2 recombinant proteins omnia kinase assay buffer
    Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in <t>Brij58</t> containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.
    Chek2 Recombinant Proteins Omnia Kinase Assay Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher novex tbe hi density sample buffer
    Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in <t>Brij58</t> containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.
    Novex Tbe Hi Density Sample Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 5x buffer
    Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in <t>Brij58</t> containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.
    5x Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 5×t4 pnk buffer a
    Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in <t>Brij58</t> containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.
    5×T4 Pnk Buffer A, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher rnalater solution
    Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in <t>Brij58</t> containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.
    Rnalater Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dsf buffer
    Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in <t>Brij58</t> containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.
    Dsf Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in Brij58 containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.

    Journal: The Journal of Experimental Medicine

    Article Title: T Cell Receptor (TCR) Interacting Molecule (TRIM), A Novel Disulfide-linked Dimer Associated with the TCR-CD3-? Complex, Recruits Intracellular Signaling Proteins to the Plasma Membrane

    doi:

    Figure Lengend Snippet: Characterization of pp29/30. ( A ) Left , Anti-pp29/30 Western blot analysis ( WB ) of HPB-ALL lysates (5 × 10 5 / lane) under nonreducing and reducing conditions. Crude pp29/ 30 antiserum or preimmune serum was used at a 1:500 vol/vol dilution. For detection, we used the ECL system ( Amersham Buchler ). Right , and B, Reprecipitation experiments ( RP ). A CD3 (OKT-3) immunoprecipitate was prepared from HPB-ALL cells (10 7 ) lysed in Brij58 containing lysis buffer and subjected to in vitro kinase assay. Phosphorylated proteins were subsequently released using Triton X-100 lysis buffer. 10% of the material was directly separated on two-dimensional IEF/SDS-PAGE ( B , left ). The remaining material was subjected to reprecipitation using preimmune serum or anti-pp29/30 immune serum. The precipitates were run on nonreducing or reducing 12% SDS-PAGE ( A , right ) or were separated on two-dimensional reducing IEF/SDS-PAGE ( B , middle and right ). Radiolabeled proteins were visualized by autoradiography.

    Article Snippet: 5 × 109 HPB-ALL cells were washed once in TBS and lysed in 70 ml of 1% Brij58 lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% Brij58 ( Pierce Chemical Co. , Rockford, IL), 1 mM NaVanadate, 10 mM NaF, 1 mM PMSF, 1 μg/ml aprotinin, and 1 μg/ml leupeptin) at 4°C for 2 h. Postnuclear lysates were consecutively incubated with two aliquots of CNBr-activated Sepharose beads (3 ml packed beads) coupled with the protein A–purified CD3 mAb OKT-3 for 1 h. The immune complexes were subsequently spun down, and the beads from the first and second immunoprecipitations were pooled and washed four times with 10 ml washing buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM NaF, 1 mM PMSF, 0.1% Brij58).

    Techniques: Western Blot, Lysis, In Vitro, Kinase Assay, Electrofocusing, SDS Page, Autoradiography