polymerase chain reaction pcr buffer 10x Search Results


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  • 99
    Thermo Fisher polymerase chain reaction pcr buffer
    Detection of nptII gene in samples (Results of <t>PCR</t> products of primer pairs (nptIIf-nptIIr)) M: 100 bp <t>DNA</t> marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.
    Polymerase Chain Reaction Pcr Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore pcr buffer
    Twist1 expression in murine and human GC cell lines. (A) <t>RT-PCR</t> analysis of Twist1 mRNA expression in five mouse GC cell lines MDGC-1, MDGC-3, MDGC-7, MDGC-8 and MDGC-9) from DCKO mice. Mouse Gapdh was used as an internal control. (B) Expression of Twist1 protein in MDGC cells by Western blot. α-tubulin was used as an internal control. (C and D) Effects of a <t>DNA</t> demethylation agent in MDGC cells. After treatment with 5-aza-dC, Twist1 expression was up-regulated at the mRNA (C) and protein levels (D) in Twist1 expression-negative MDGC-1 and MDGC-3 cells, but not in Twist1 expression-positive MDGC-9 cells. M, mock; A, 5-aza-dC. (E) RT-PCR analysis of Twist1 mRNA expression in human GC cell lines (KATO-III, GCIY, AGS, MKN74, MKN7, MKN45, NUGC4 and HSC60) (left). Twist1 expression was up-regulated at the mRNA level in KATO-III and GCIY cells after 5-aza-dC treatment (right). Human GAPDH was used as an internal control. (F) qRT-PCR analysis of Twist1 mRNA expression in MDGC-9, MKN7 and MKN45 cells with and without 5-aza-dC treatment (**P
    Pcr Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 14610 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr buffer/product/Millipore
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    90
    TaKaRa polymerase chain reaction pcr buffer 10x
    Twist1 expression in murine and human GC cell lines. (A) <t>RT-PCR</t> analysis of Twist1 mRNA expression in five mouse GC cell lines MDGC-1, MDGC-3, MDGC-7, MDGC-8 and MDGC-9) from DCKO mice. Mouse Gapdh was used as an internal control. (B) Expression of Twist1 protein in MDGC cells by Western blot. α-tubulin was used as an internal control. (C and D) Effects of a <t>DNA</t> demethylation agent in MDGC cells. After treatment with 5-aza-dC, Twist1 expression was up-regulated at the mRNA (C) and protein levels (D) in Twist1 expression-negative MDGC-1 and MDGC-3 cells, but not in Twist1 expression-positive MDGC-9 cells. M, mock; A, 5-aza-dC. (E) RT-PCR analysis of Twist1 mRNA expression in human GC cell lines (KATO-III, GCIY, AGS, MKN74, MKN7, MKN45, NUGC4 and HSC60) (left). Twist1 expression was up-regulated at the mRNA level in KATO-III and GCIY cells after 5-aza-dC treatment (right). Human GAPDH was used as an internal control. (F) qRT-PCR analysis of Twist1 mRNA expression in MDGC-9, MKN7 and MKN45 cells with and without 5-aza-dC treatment (**P
    Polymerase Chain Reaction Pcr Buffer 10x, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher polymerase chain reaction pcr taq polymerase
    Twist1 expression in murine and human GC cell lines. (A) <t>RT-PCR</t> analysis of Twist1 mRNA expression in five mouse GC cell lines MDGC-1, MDGC-3, MDGC-7, MDGC-8 and MDGC-9) from DCKO mice. Mouse Gapdh was used as an internal control. (B) Expression of Twist1 protein in MDGC cells by Western blot. α-tubulin was used as an internal control. (C and D) Effects of a <t>DNA</t> demethylation agent in MDGC cells. After treatment with 5-aza-dC, Twist1 expression was up-regulated at the mRNA (C) and protein levels (D) in Twist1 expression-negative MDGC-1 and MDGC-3 cells, but not in Twist1 expression-positive MDGC-9 cells. M, mock; A, 5-aza-dC. (E) RT-PCR analysis of Twist1 mRNA expression in human GC cell lines (KATO-III, GCIY, AGS, MKN74, MKN7, MKN45, NUGC4 and HSC60) (left). Twist1 expression was up-regulated at the mRNA level in KATO-III and GCIY cells after 5-aza-dC treatment (right). Human GAPDH was used as an internal control. (F) qRT-PCR analysis of Twist1 mRNA expression in MDGC-9, MKN7 and MKN45 cells with and without 5-aza-dC treatment (**P
    Polymerase Chain Reaction Pcr Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 42 article reviews
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    94
    Roche pcr buffer
    Colony <t>PCR</t> showing the <t>cDNA</t> derived maternal and paternal fragment of Nvtra . Panels A – D and E – H indicate the four replicate PCR runs. The maternal origin and age of the embryos is indicated above each set of PCR fragments. White arrow indicates cDNA fragment, black arrow indicates Russia Bait cDNA fragment and dotted arrow indicates gDNA fragment. M is 100 bp molecular standard, ranging from 300 bp (lowest band) to 500 bp (higest band).
    Pcr Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 5189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore toptaq pcr buffer
    Colony <t>PCR</t> showing the <t>cDNA</t> derived maternal and paternal fragment of Nvtra . Panels A – D and E – H indicate the four replicate PCR runs. The maternal origin and age of the embryos is indicated above each set of PCR fragments. White arrow indicates cDNA fragment, black arrow indicates Russia Bait cDNA fragment and dotted arrow indicates gDNA fragment. M is 100 bp molecular standard, ranging from 300 bp (lowest band) to 500 bp (higest band).
    Toptaq Pcr Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Promega pcr buffer
    Colony <t>PCR</t> showing the <t>cDNA</t> derived maternal and paternal fragment of Nvtra . Panels A – D and E – H indicate the four replicate PCR runs. The maternal origin and age of the embryos is indicated above each set of PCR fragments. White arrow indicates cDNA fragment, black arrow indicates Russia Bait cDNA fragment and dotted arrow indicates gDNA fragment. M is 100 bp molecular standard, ranging from 300 bp (lowest band) to 500 bp (higest band).
    Pcr Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 7741 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 7741 article reviews
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    95
    TaKaRa pcr buffer
    Colony <t>PCR</t> showing the <t>cDNA</t> derived maternal and paternal fragment of Nvtra . Panels A – D and E – H indicate the four replicate PCR runs. The maternal origin and age of the embryos is indicated above each set of PCR fragments. White arrow indicates cDNA fragment, black arrow indicates Russia Bait cDNA fragment and dotted arrow indicates gDNA fragment. M is 100 bp molecular standard, ranging from 300 bp (lowest band) to 500 bp (higest band).
    Pcr Buffer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 10602 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Stratagene pcr buffer
    Colony <t>PCR</t> showing the <t>cDNA</t> derived maternal and paternal fragment of Nvtra . Panels A – D and E – H indicate the four replicate PCR runs. The maternal origin and age of the embryos is indicated above each set of PCR fragments. White arrow indicates cDNA fragment, black arrow indicates Russia Bait cDNA fragment and dotted arrow indicates gDNA fragment. M is 100 bp molecular standard, ranging from 300 bp (lowest band) to 500 bp (higest band).
    Pcr Buffer, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 334 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    PEQLAB pcr buffer
    Colony <t>PCR</t> showing the <t>cDNA</t> derived maternal and paternal fragment of Nvtra . Panels A – D and E – H indicate the four replicate PCR runs. The maternal origin and age of the embryos is indicated above each set of PCR fragments. White arrow indicates cDNA fragment, black arrow indicates Russia Bait cDNA fragment and dotted arrow indicates gDNA fragment. M is 100 bp molecular standard, ranging from 300 bp (lowest band) to 500 bp (higest band).
    Pcr Buffer, supplied by PEQLAB, used in various techniques. Bioz Stars score: 92/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sequenom pcr buffer
    Colony <t>PCR</t> showing the <t>cDNA</t> derived maternal and paternal fragment of Nvtra . Panels A – D and E – H indicate the four replicate PCR runs. The maternal origin and age of the embryos is indicated above each set of PCR fragments. White arrow indicates cDNA fragment, black arrow indicates Russia Bait cDNA fragment and dotted arrow indicates gDNA fragment. M is 100 bp molecular standard, ranging from 300 bp (lowest band) to 500 bp (higest band).
    Pcr Buffer, supplied by Sequenom, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Kaneka Corp pcr buffer
    Colony <t>PCR</t> showing the <t>cDNA</t> derived maternal and paternal fragment of Nvtra . Panels A – D and E – H indicate the four replicate PCR runs. The maternal origin and age of the embryos is indicated above each set of PCR fragments. White arrow indicates cDNA fragment, black arrow indicates Russia Bait cDNA fragment and dotted arrow indicates gDNA fragment. M is 100 bp molecular standard, ranging from 300 bp (lowest band) to 500 bp (higest band).
    Pcr Buffer, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 93/100, based on 274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pcr  (Qiagen)
    99
    Qiagen pcr
    Colony <t>PCR</t> showing the <t>cDNA</t> derived maternal and paternal fragment of Nvtra . Panels A – D and E – H indicate the four replicate PCR runs. The maternal origin and age of the embryos is indicated above each set of PCR fragments. White arrow indicates cDNA fragment, black arrow indicates Russia Bait cDNA fragment and dotted arrow indicates gDNA fragment. M is 100 bp molecular standard, ranging from 300 bp (lowest band) to 500 bp (higest band).
    Pcr, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 35685 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Toyobo pcr buffer
    Quantification of <t>HPV16</t> viral copy number by real-time <t>PCR.</t> (A) Standard curve indicating CT value vs. HPV16 copy number. A standard curve was calculated by plotting obtained CT values against serial 10-fold dilutions produced with HPV16 copy numbers ranging from 100 to 109. (B) The average number of viral copies was significantly higher in the gargle samples as compared to the oral rinse samples (0.16±0.27 vs. 1.35±1.26 per cell) (***P
    Pcr Buffer, supplied by Toyobo, used in various techniques. Bioz Stars score: 93/100, based on 1108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Detection of nptII gene in samples (Results of PCR products of primer pairs (nptIIf-nptIIr)) M: 100 bp DNA marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of nptII gene in samples (Results of PCR products of primer pairs (nptIIf-nptIIr)) M: 100 bp DNA marker, B: negative control, P: positive control plasmid (pBI-121), lanes A1–P2: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Plasmid Preparation

    Detection of epsps gene in some samples tested (Results of PCR products of primer pairs (GMO9/GMO5)) M: 100 bp DNA marker, B: negative control, lanes A1–M10: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of epsps gene in some samples tested (Results of PCR products of primer pairs (GMO9/GMO5)) M: 100 bp DNA marker, B: negative control, lanes A1–M10: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control

    Detection of epsps gene in samples (Results of PCR products of primer pairs: GMO5, GMO9) M: 100 bp DNA marker, B: negative control, lanes A1–P2: Tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of epsps gene in samples (Results of PCR products of primer pairs: GMO5, GMO9) M: 100 bp DNA marker, B: negative control, lanes A1–P2: Tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control

    Detection of lectin gene in soybean samples tested (A1, A2, A3, A4, A5, and A6) (Results of PCR products of primer pair (GM03/GM04)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of lectin gene in soybean samples tested (A1, A2, A3, A4, A5, and A6) (Results of PCR products of primer pair (GM03/GM04)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control

    Detection of 35S promoter in samples (Results of PCR products of primer pairs p35S-cf3 and p35S-cf4), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of 35S promoter in samples (Results of PCR products of primer pairs p35S-cf3 and p35S-cf4), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Plasmid Preparation

    Detection of zein gene in maize samples tested (M1, M2, M3, M5, M6, M7, M8, M9, M10, M11, M12, M13, M14, M15, and M16) (Results of PCR products of primer pair zein3/zein4), M: 100 bp DNA marker, B: negative control, lanes A1–P2: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of zein gene in maize samples tested (M1, M2, M3, M5, M6, M7, M8, M9, M10, M11, M12, M13, M14, M15, and M16) (Results of PCR products of primer pair zein3/zein4), M: 100 bp DNA marker, B: negative control, lanes A1–P2: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control

    Detection of nos terminator in samples (Results of PCR products of primer pairs HA-nos118-f/HA-nos118-r), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of nos terminator in samples (Results of PCR products of primer pairs HA-nos118-f/HA-nos118-r), M: 100 bp DNA marker, B: negative control, P: positive control plasmid (PGIIMH35-2PS), lanes A1-R: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Polymerase Chain Reaction, Marker, Negative Control, Positive Control, Plasmid Preparation

    Detection of epsps gene in some samples tested by nested PCR (Results of PCR products of primer pairs (GMO8/GMO7)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

    Journal: Data in Brief

    Article Title: Data in support of the detection of genetically modified organisms (GMOs) in food and feed samples

    doi: 10.1016/j.dib.2016.02.035

    Figure Lengend Snippet: Detection of epsps gene in some samples tested by nested PCR (Results of PCR products of primer pairs (GMO8/GMO7)) M: 100 bp DNA marker, B: negative control, lanes A1–A6: tested samples.

    Article Snippet: The final concentrations of each PCR were as follows: l× of 10× PCR buffer (fermentase); 100 ng of genomic DNA; 0.4 pm of each primers; 0.32 mM of dNTPs mix; 2 mM MgCl2 ; 0.5 unit/reaction of (Fermentas) Taq DNA polymerase.

    Techniques: Nested PCR, Polymerase Chain Reaction, Marker, Negative Control

    Twist1 expression in murine and human GC cell lines. (A) RT-PCR analysis of Twist1 mRNA expression in five mouse GC cell lines MDGC-1, MDGC-3, MDGC-7, MDGC-8 and MDGC-9) from DCKO mice. Mouse Gapdh was used as an internal control. (B) Expression of Twist1 protein in MDGC cells by Western blot. α-tubulin was used as an internal control. (C and D) Effects of a DNA demethylation agent in MDGC cells. After treatment with 5-aza-dC, Twist1 expression was up-regulated at the mRNA (C) and protein levels (D) in Twist1 expression-negative MDGC-1 and MDGC-3 cells, but not in Twist1 expression-positive MDGC-9 cells. M, mock; A, 5-aza-dC. (E) RT-PCR analysis of Twist1 mRNA expression in human GC cell lines (KATO-III, GCIY, AGS, MKN74, MKN7, MKN45, NUGC4 and HSC60) (left). Twist1 expression was up-regulated at the mRNA level in KATO-III and GCIY cells after 5-aza-dC treatment (right). Human GAPDH was used as an internal control. (F) qRT-PCR analysis of Twist1 mRNA expression in MDGC-9, MKN7 and MKN45 cells with and without 5-aza-dC treatment (**P

    Journal: PLoS ONE

    Article Title: DNA Methylation in the Exon 1 Region and Complex Regulation of Twist1 Expression in Gastric Cancer Cells

    doi: 10.1371/journal.pone.0145630

    Figure Lengend Snippet: Twist1 expression in murine and human GC cell lines. (A) RT-PCR analysis of Twist1 mRNA expression in five mouse GC cell lines MDGC-1, MDGC-3, MDGC-7, MDGC-8 and MDGC-9) from DCKO mice. Mouse Gapdh was used as an internal control. (B) Expression of Twist1 protein in MDGC cells by Western blot. α-tubulin was used as an internal control. (C and D) Effects of a DNA demethylation agent in MDGC cells. After treatment with 5-aza-dC, Twist1 expression was up-regulated at the mRNA (C) and protein levels (D) in Twist1 expression-negative MDGC-1 and MDGC-3 cells, but not in Twist1 expression-positive MDGC-9 cells. M, mock; A, 5-aza-dC. (E) RT-PCR analysis of Twist1 mRNA expression in human GC cell lines (KATO-III, GCIY, AGS, MKN74, MKN7, MKN45, NUGC4 and HSC60) (left). Twist1 expression was up-regulated at the mRNA level in KATO-III and GCIY cells after 5-aza-dC treatment (right). Human GAPDH was used as an internal control. (F) qRT-PCR analysis of Twist1 mRNA expression in MDGC-9, MKN7 and MKN45 cells with and without 5-aza-dC treatment (**P

    Article Snippet: Briefly, the PCR reaction was performed for 35 cycles in a 25 μl mixture comprising bisulfite-modified DNA, 2.5μl of 10x PCR buffer, 1.25 μl of 25mM dNTPs, 25 pmol/l of each primer and 1 U of JumpStart RedTaq polymerase (Sigma; #D0563).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Mouse Assay, Western Blot, Quantitative RT-PCR

    Colony PCR showing the cDNA derived maternal and paternal fragment of Nvtra . Panels A – D and E – H indicate the four replicate PCR runs. The maternal origin and age of the embryos is indicated above each set of PCR fragments. White arrow indicates cDNA fragment, black arrow indicates Russia Bait cDNA fragment and dotted arrow indicates gDNA fragment. M is 100 bp molecular standard, ranging from 300 bp (lowest band) to 500 bp (higest band).

    Journal: PLoS ONE

    Article Title: A New Component of the Nasonia Sex Determining Cascade Is Maternally Silenced and Regulates Transformer Expression

    doi: 10.1371/journal.pone.0063618

    Figure Lengend Snippet: Colony PCR showing the cDNA derived maternal and paternal fragment of Nvtra . Panels A – D and E – H indicate the four replicate PCR runs. The maternal origin and age of the embryos is indicated above each set of PCR fragments. White arrow indicates cDNA fragment, black arrow indicates Russia Bait cDNA fragment and dotted arrow indicates gDNA fragment. M is 100 bp molecular standard, ranging from 300 bp (lowest band) to 500 bp (higest band).

    Article Snippet: One l 1∶100 diluted cDNA was mixed with 10x PCR buffer (Roche) (10x concentrate; 100 mM Tris-HCl, 500 mM KCl; pH 8.3), 200 M dNTPs, 2 units Taq polymerase (Roche), 400 nM of primers NvTra_poly_F1 and NvTra_poly_R1 and supplemented with milliQ water to 25 l. PCR profile was as follows: initial denaturing step of 95 C for 3 min, followed by 20 cycles of 95 C for 15 s, 57 C for 30 s and 72 C for 30 s, ending with a final extension step of 72 C for 7 min. As negative control one l of milliQ water was used.

    Techniques: Polymerase Chain Reaction, Derivative Assay

    Quantification of HPV16 viral copy number by real-time PCR. (A) Standard curve indicating CT value vs. HPV16 copy number. A standard curve was calculated by plotting obtained CT values against serial 10-fold dilutions produced with HPV16 copy numbers ranging from 100 to 109. (B) The average number of viral copies was significantly higher in the gargle samples as compared to the oral rinse samples (0.16±0.27 vs. 1.35±1.26 per cell) (***P

    Journal: Journal of Applied Oral Science

    Article Title: Higher prevalence and gene amplification of HPV16 in oropharynx as compared to oral cavity

    doi: 10.1590/1678-775720160009

    Figure Lengend Snippet: Quantification of HPV16 viral copy number by real-time PCR. (A) Standard curve indicating CT value vs. HPV16 copy number. A standard curve was calculated by plotting obtained CT values against serial 10-fold dilutions produced with HPV16 copy numbers ranging from 100 to 109. (B) The average number of viral copies was significantly higher in the gargle samples as compared to the oral rinse samples (0.16±0.27 vs. 1.35±1.26 per cell) (***P

    Article Snippet: Each of those mixtures was amplified with 10x PCR buffer (TOYOBO), dNTPs, Taq DNA polymerase, and HPV16 primers.

    Techniques: Real-time Polymerase Chain Reaction, Produced

    Detection of HPV16 by PCR. (A) Standard curve indicating CT value vs. ERV3-1 copy number. A standard curve was calculated by plotting obtained CT values against serial 10-fold dilutions produced with ERV3-1 copy numbers ranging from 100 to 109. (B) Agarose gel electrophoresis of amplified PCR products from cell lines and oral samples. Ten microliters of each 20-μl PCR product were separated on a 3.0% agarose gel. HPV16 DNA was detected in Caski cells (HPV16 positive), but not in HTB-31 cells (HPV negative). Lanes: 1-5, oral rinse samples (C) PCR products were examined by sequencing for the HPV16 E6 region

    Journal: Journal of Applied Oral Science

    Article Title: Higher prevalence and gene amplification of HPV16 in oropharynx as compared to oral cavity

    doi: 10.1590/1678-775720160009

    Figure Lengend Snippet: Detection of HPV16 by PCR. (A) Standard curve indicating CT value vs. ERV3-1 copy number. A standard curve was calculated by plotting obtained CT values against serial 10-fold dilutions produced with ERV3-1 copy numbers ranging from 100 to 109. (B) Agarose gel electrophoresis of amplified PCR products from cell lines and oral samples. Ten microliters of each 20-μl PCR product were separated on a 3.0% agarose gel. HPV16 DNA was detected in Caski cells (HPV16 positive), but not in HTB-31 cells (HPV negative). Lanes: 1-5, oral rinse samples (C) PCR products were examined by sequencing for the HPV16 E6 region

    Article Snippet: Each of those mixtures was amplified with 10x PCR buffer (TOYOBO), dNTPs, Taq DNA polymerase, and HPV16 primers.

    Techniques: Polymerase Chain Reaction, Produced, Agarose Gel Electrophoresis, Amplification, Sequencing