polymerase buffer Qiagen Search Results


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  • 95
    Qiagen taq polymerase
    Taq Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 5083 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen qiagen ae buffer
    s-LA versus m-LA with <t>Qiagen</t> Spin blood kit <t>DNA</t> extraction.
    Qiagen Ae Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Qiagen dna polymerase buffer
    s-LA versus m-LA with <t>Qiagen</t> Spin blood kit <t>DNA</t> extraction.
    Dna Polymerase Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffer avl
    ELISAs to demonstrate inactivation efficacy of Buffer <t>AVL,</t> ethanol, or heat alone or in combination on <t>EBOV-Kikwit.</t> Replicate samples of EBOV-Kikwit-infected marmoset serum or EBOV-Kikwit-spiked blood were treated as described below for individual panels.
    Buffer Avl, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen eb buffer
    ELISAs to demonstrate inactivation efficacy of Buffer <t>AVL,</t> ethanol, or heat alone or in combination on <t>EBOV-Kikwit.</t> Replicate samples of EBOV-Kikwit-infected marmoset serum or EBOV-Kikwit-spiked blood were treated as described below for individual panels.
    Eb Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1949 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen pb dna binding buffer
    ELISAs to demonstrate inactivation efficacy of Buffer <t>AVL,</t> ethanol, or heat alone or in combination on <t>EBOV-Kikwit.</t> Replicate samples of EBOV-Kikwit-infected marmoset serum or EBOV-Kikwit-spiked blood were treated as described below for individual panels.
    Pb Dna Binding Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen pe buffer
    ELISAs to demonstrate inactivation efficacy of Buffer <t>AVL,</t> ethanol, or heat alone or in combination on <t>EBOV-Kikwit.</t> Replicate samples of EBOV-Kikwit-infected marmoset serum or EBOV-Kikwit-spiked blood were treated as described below for individual panels.
    Pe Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffer rlt
    <t>RNA</t> integrity of some RNA samples after enrichment with buffer <t>RLT,</t> Triton X-100 and bead beating. Electropherogram profiles were determined with a Fragment Analyzer. Quality for Fragment Analyzer is shown as the RQN value. Reported on a scale of 1 to 10, with higher values indicating a better quality of total RNA. Values above 7 are considered to represent high quality and non-degraded RNA.
    Buffer Rlt, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 4063 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen qiagen asl buffer
    <t>RNA</t> integrity of some RNA samples after enrichment with buffer <t>RLT,</t> Triton X-100 and bead beating. Electropherogram profiles were determined with a Fragment Analyzer. Quality for Fragment Analyzer is shown as the RQN value. Reported on a scale of 1 to 10, with higher values indicating a better quality of total RNA. Values above 7 are considered to represent high quality and non-degraded RNA.
    Qiagen Asl Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffer qf
    <t>RNA</t> integrity of some RNA samples after enrichment with buffer <t>RLT,</t> Triton X-100 and bead beating. Electropherogram profiles were determined with a Fragment Analyzer. Quality for Fragment Analyzer is shown as the RQN value. Reported on a scale of 1 to 10, with higher values indicating a better quality of total RNA. Values above 7 are considered to represent high quality and non-degraded RNA.
    Buffer Qf, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffer pm
    <t>RNA</t> integrity of some RNA samples after enrichment with buffer <t>RLT,</t> Triton X-100 and bead beating. Electropherogram profiles were determined with a Fragment Analyzer. Quality for Fragment Analyzer is shown as the RQN value. Reported on a scale of 1 to 10, with higher values indicating a better quality of total RNA. Values above 7 are considered to represent high quality and non-degraded RNA.
    Buffer Pm, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffer tcl
    <t>RNA</t> integrity of some RNA samples after enrichment with buffer <t>RLT,</t> Triton X-100 and bead beating. Electropherogram profiles were determined with a Fragment Analyzer. Quality for Fragment Analyzer is shown as the RQN value. Reported on a scale of 1 to 10, with higher values indicating a better quality of total RNA. Values above 7 are considered to represent high quality and non-degraded RNA.
    Buffer Tcl, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffer g2
    <t>RNA</t> integrity of some RNA samples after enrichment with buffer <t>RLT,</t> Triton X-100 and bead beating. Electropherogram profiles were determined with a Fragment Analyzer. Quality for Fragment Analyzer is shown as the RQN value. Reported on a scale of 1 to 10, with higher values indicating a better quality of total RNA. Values above 7 are considered to represent high quality and non-degraded RNA.
    Buffer G2, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffer qc
    <t>RNA</t> integrity of some RNA samples after enrichment with buffer <t>RLT,</t> Triton X-100 and bead beating. Electropherogram profiles were determined with a Fragment Analyzer. Quality for Fragment Analyzer is shown as the RQN value. Reported on a scale of 1 to 10, with higher values indicating a better quality of total RNA. Values above 7 are considered to represent high quality and non-degraded RNA.
    Buffer Qc, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen atl buffer
    PCR-RFLP identification of Leishmania species from cutaneous leishmaniasis lesions. (A) <t>DNA</t> extracted from <t>ATL</t> buffer, CL05 and CL06 produced the RFLP pattern characteristic of Leishmania major , with band sizes as described in the text. (B) DNA extracted from culture of the isolate from CL08 (MHOM/SD/2017/ELOBIED) also gave the pattern typical of L. major ; PCR-RFLP of L. tropica was included as control. The L. major identification was verified by DNA sequencing (see text).
    Atl Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffer qg
    PCR-RFLP identification of Leishmania species from cutaneous leishmaniasis lesions. (A) <t>DNA</t> extracted from <t>ATL</t> buffer, CL05 and CL06 produced the RFLP pattern characteristic of Leishmania major , with band sizes as described in the text. (B) DNA extracted from culture of the isolate from CL08 (MHOM/SD/2017/ELOBIED) also gave the pattern typical of L. major ; PCR-RFLP of L. tropica was included as control. The L. major identification was verified by DNA sequencing (see text).
    Buffer Qg, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffer qbt
    PCR-RFLP identification of Leishmania species from cutaneous leishmaniasis lesions. (A) <t>DNA</t> extracted from <t>ATL</t> buffer, CL05 and CL06 produced the RFLP pattern characteristic of Leishmania major , with band sizes as described in the text. (B) DNA extracted from culture of the isolate from CL08 (MHOM/SD/2017/ELOBIED) also gave the pattern typical of L. major ; PCR-RFLP of L. tropica was included as control. The L. major identification was verified by DNA sequencing (see text).
    Buffer Qbt, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen ap1 buffer
    PCR-RFLP identification of Leishmania species from cutaneous leishmaniasis lesions. (A) <t>DNA</t> extracted from <t>ATL</t> buffer, CL05 and CL06 produced the RFLP pattern characteristic of Leishmania major , with band sizes as described in the text. (B) DNA extracted from culture of the isolate from CL08 (MHOM/SD/2017/ELOBIED) also gave the pattern typical of L. major ; PCR-RFLP of L. tropica was included as control. The L. major identification was verified by DNA sequencing (see text).
    Ap1 Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffer aw2
    Optimization of the DNA extraction procedure. Individual Cq values with median after the duplex qPCR amplification of DNA extracted from T. equi -infected equine blood according to the standard protocol provided with the DNA extraction kit or with modifications that involved preliminary lysis of erythrocytes with RBC Lysis Solution or additional wash of the spin column containing adsorbed DNA with either Buffer AW1 (AW1 Plus) or Buffer <t>AW2</t> (AW2 Plus). These samples were amplified either with or without BSA in the reaction mixture. Graph was produced using Prism 6
    Buffer Aw2, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffer aw1
    Optimization of the DNA extraction procedure. Individual Cq values with median after the duplex qPCR amplification of DNA extracted from T. equi -infected equine blood according to the standard protocol provided with the DNA extraction kit or with modifications that involved preliminary lysis of erythrocytes with RBC Lysis Solution or additional wash of the spin column containing adsorbed DNA with either Buffer <t>AW1</t> (AW1 Plus) or Buffer AW2 (AW2 Plus). These samples were amplified either with or without BSA in the reaction mixture. Graph was produced using Prism 6
    Buffer Aw1, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rdd buffer
    Optimization of the DNA extraction procedure. Individual Cq values with median after the duplex qPCR amplification of DNA extracted from T. equi -infected equine blood according to the standard protocol provided with the DNA extraction kit or with modifications that involved preliminary lysis of erythrocytes with RBC Lysis Solution or additional wash of the spin column containing adsorbed DNA with either Buffer <t>AW1</t> (AW1 Plus) or Buffer AW2 (AW2 Plus). These samples were amplified either with or without BSA in the reaction mixture. Graph was produced using Prism 6
    Rdd Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen one step rt pcr
    A 2% agarose gel (0.5 μg/mL EtBr) of amplified DNA from <t>RT-PCR</t> of <t>RNA</t> isolated from cells after XPF (left) and XPG (right) knockdown. 1×TBE was used for the gel and running buffer. The gel was run at 100 V for 120 min.
    One Step Rt Pcr, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1518 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen qiagen al buffer
    Comparison of AOM and <t>Qiagen</t> extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using <t>HSV-positive</t> CSF. Neat HSV1-PC + was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines . The QDM extracted HSV-1 DNA from the serial dilutions was amplified and detected in AOM tubes ( dashed lines ). HSV-negative CSF was extracted by the AOM method and represented by the light solid line . AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.
    Qiagen Al Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen buffers rwt
    Comparison of AOM and <t>Qiagen</t> extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using <t>HSV-positive</t> CSF. Neat HSV1-PC + was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines . The QDM extracted HSV-1 DNA from the serial dilutions was amplified and detected in AOM tubes ( dashed lines ). HSV-negative CSF was extracted by the AOM method and represented by the light solid line . AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.
    Buffers Rwt, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen ave buffer
    Comparison of AOM and <t>Qiagen</t> extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using <t>HSV-positive</t> CSF. Neat HSV1-PC + was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines . The QDM extracted HSV-1 DNA from the serial dilutions was amplified and detected in AOM tubes ( dashed lines ). HSV-negative CSF was extracted by the AOM method and represented by the light solid line . AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.
    Ave Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen pyromark washing buffer
    Comparison of AOM and <t>Qiagen</t> extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using <t>HSV-positive</t> CSF. Neat HSV1-PC + was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines . The QDM extracted HSV-1 DNA from the serial dilutions was amplified and detected in AOM tubes ( dashed lines ). HSV-negative CSF was extracted by the AOM method and represented by the light solid line . AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.
    Pyromark Washing Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rlt plus rneasy lysis buffer
    Comparison of AOM and <t>Qiagen</t> extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using <t>HSV-positive</t> CSF. Neat HSV1-PC + was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines . The QDM extracted HSV-1 DNA from the serial dilutions was amplified and detected in AOM tubes ( dashed lines ). HSV-negative CSF was extracted by the AOM method and represented by the light solid line . AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.
    Rlt Plus Rneasy Lysis Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen toptaq dna polymerase amplification buffer
    Comparison of AOM and <t>Qiagen</t> extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using <t>HSV-positive</t> CSF. Neat HSV1-PC + was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines . The QDM extracted HSV-1 DNA from the serial dilutions was amplified and detected in AOM tubes ( dashed lines ). HSV-negative CSF was extracted by the AOM method and represented by the light solid line . AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.
    Toptaq Dna Polymerase Amplification Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen qiagen multiplex kit
    Comparison of AOM and <t>Qiagen</t> extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using <t>HSV-positive</t> CSF. Neat HSV1-PC + was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines . The QDM extracted HSV-1 DNA from the serial dilutions was amplified and detected in AOM tubes ( dashed lines ). HSV-negative CSF was extracted by the AOM method and represented by the light solid line . AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.
    Qiagen Multiplex Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 318 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rw1 buffer
    Comparison of AOM and <t>Qiagen</t> extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using <t>HSV-positive</t> CSF. Neat HSV1-PC + was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines . The QDM extracted HSV-1 DNA from the serial dilutions was amplified and detected in AOM tubes ( dashed lines ). HSV-negative CSF was extracted by the AOM method and represented by the light solid line . AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.
    Rw1 Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen polymerase buffer
    Comparison of AOM and <t>Qiagen</t> extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using <t>HSV-positive</t> CSF. Neat HSV1-PC + was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines . The QDM extracted HSV-1 DNA from the serial dilutions was amplified and detected in AOM tubes ( dashed lines ). HSV-negative CSF was extracted by the AOM method and represented by the light solid line . AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.
    Polymerase Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    s-LA versus m-LA with Qiagen Spin blood kit DNA extraction.

    Journal: Journal of Clinical Microbiology

    Article Title: Comparison of Real-Time Multiplex Human Papillomavirus (HPV) PCR Assays with the Linear Array HPV Genotyping PCR Assay and Influence of DNA Extraction Method on HPV Detection ▿Comparison of Real-Time Multiplex Human Papillomavirus (HPV) PCR Assays with the Linear Array HPV Genotyping PCR Assay and Influence of DNA Extraction Method on HPV Detection ▿ ‡

    doi: 10.1128/JCM.00235-10

    Figure Lengend Snippet: s-LA versus m-LA with Qiagen Spin blood kit DNA extraction.

    Article Snippet: The purified DNA was eluted in 200 μl of Qiagen AE buffer.

    Techniques: DNA Extraction

    Multiplex HPV PCR versus the standard Linear Array with Qiagen MinElute media kit DNA extraction.

    Journal: Journal of Clinical Microbiology

    Article Title: Comparison of Real-Time Multiplex Human Papillomavirus (HPV) PCR Assays with the Linear Array HPV Genotyping PCR Assay and Influence of DNA Extraction Method on HPV Detection ▿Comparison of Real-Time Multiplex Human Papillomavirus (HPV) PCR Assays with the Linear Array HPV Genotyping PCR Assay and Influence of DNA Extraction Method on HPV Detection ▿ ‡

    doi: 10.1128/JCM.00235-10

    Figure Lengend Snippet: Multiplex HPV PCR versus the standard Linear Array with Qiagen MinElute media kit DNA extraction.

    Article Snippet: The purified DNA was eluted in 200 μl of Qiagen AE buffer.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, DNA Extraction

    Multiplex HPV PCR versus m-LA with Qiagen Spin blood kit DNA extraction.

    Journal: Journal of Clinical Microbiology

    Article Title: Comparison of Real-Time Multiplex Human Papillomavirus (HPV) PCR Assays with the Linear Array HPV Genotyping PCR Assay and Influence of DNA Extraction Method on HPV Detection ▿Comparison of Real-Time Multiplex Human Papillomavirus (HPV) PCR Assays with the Linear Array HPV Genotyping PCR Assay and Influence of DNA Extraction Method on HPV Detection ▿ ‡

    doi: 10.1128/JCM.00235-10

    Figure Lengend Snippet: Multiplex HPV PCR versus m-LA with Qiagen Spin blood kit DNA extraction.

    Article Snippet: The purified DNA was eluted in 200 μl of Qiagen AE buffer.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, DNA Extraction

    Multiplex HPV PCR versus s-LA with Qiagen Spin blood kit DNA extraction.

    Journal: Journal of Clinical Microbiology

    Article Title: Comparison of Real-Time Multiplex Human Papillomavirus (HPV) PCR Assays with the Linear Array HPV Genotyping PCR Assay and Influence of DNA Extraction Method on HPV Detection ▿Comparison of Real-Time Multiplex Human Papillomavirus (HPV) PCR Assays with the Linear Array HPV Genotyping PCR Assay and Influence of DNA Extraction Method on HPV Detection ▿ ‡

    doi: 10.1128/JCM.00235-10

    Figure Lengend Snippet: Multiplex HPV PCR versus s-LA with Qiagen Spin blood kit DNA extraction.

    Article Snippet: The purified DNA was eluted in 200 μl of Qiagen AE buffer.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, DNA Extraction

    ELISAs to demonstrate inactivation efficacy of Buffer AVL, ethanol, or heat alone or in combination on EBOV-Kikwit. Replicate samples of EBOV-Kikwit-infected marmoset serum or EBOV-Kikwit-spiked blood were treated as described below for individual panels.

    Journal: Journal of Clinical Microbiology

    Article Title: Buffer AVL Alone Does Not Inactivate Ebola Virus in a Representative Clinical Sample Type

    doi: 10.1128/JCM.01449-15

    Figure Lengend Snippet: ELISAs to demonstrate inactivation efficacy of Buffer AVL, ethanol, or heat alone or in combination on EBOV-Kikwit. Replicate samples of EBOV-Kikwit-infected marmoset serum or EBOV-Kikwit-spiked blood were treated as described below for individual panels.

    Article Snippet: In this study, we have shown that a combination of Buffer AVL and ethanol or Buffer AVL and heat can inactivate EBOV-Kikwit in whole mouse blood samples, which we believe are likely to form a worst-case sample type in terms of both inactivation and also being able to extract PCR-quality RNA.

    Techniques: Infection

    RNA integrity of some RNA samples after enrichment with buffer RLT, Triton X-100 and bead beating. Electropherogram profiles were determined with a Fragment Analyzer. Quality for Fragment Analyzer is shown as the RQN value. Reported on a scale of 1 to 10, with higher values indicating a better quality of total RNA. Values above 7 are considered to represent high quality and non-degraded RNA.

    Journal: Scientific Reports

    Article Title: Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions

    doi: 10.1038/s41598-019-54608-x

    Figure Lengend Snippet: RNA integrity of some RNA samples after enrichment with buffer RLT, Triton X-100 and bead beating. Electropherogram profiles were determined with a Fragment Analyzer. Quality for Fragment Analyzer is shown as the RQN value. Reported on a scale of 1 to 10, with higher values indicating a better quality of total RNA. Values above 7 are considered to represent high quality and non-degraded RNA.

    Article Snippet: In addition, using qPCR and RT-qPCR, we demonstrated that after cell lysis with Buffer RLT, both fungal DNA and RNA can be further enriched by means of a centrifugation step that effectively reduced human nucleic acids.

    Techniques:

    PCR-RFLP identification of Leishmania species from cutaneous leishmaniasis lesions. (A) DNA extracted from ATL buffer, CL05 and CL06 produced the RFLP pattern characteristic of Leishmania major , with band sizes as described in the text. (B) DNA extracted from culture of the isolate from CL08 (MHOM/SD/2017/ELOBIED) also gave the pattern typical of L. major ; PCR-RFLP of L. tropica was included as control. The L. major identification was verified by DNA sequencing (see text).

    Journal: International Journal of Infectious Diseases

    Article Title: Epidemiological and molecular investigation of resurgent cutaneous leishmaniasis in Sudan

    doi: 10.1016/j.ijid.2019.08.018

    Figure Lengend Snippet: PCR-RFLP identification of Leishmania species from cutaneous leishmaniasis lesions. (A) DNA extracted from ATL buffer, CL05 and CL06 produced the RFLP pattern characteristic of Leishmania major , with band sizes as described in the text. (B) DNA extracted from culture of the isolate from CL08 (MHOM/SD/2017/ELOBIED) also gave the pattern typical of L. major ; PCR-RFLP of L. tropica was included as control. The L. major identification was verified by DNA sequencing (see text).

    Article Snippet: DNA extraction DNA extraction From ATL buffer samples and cultures was performed using the QIAamp DNA Mini Kit (51304; Qiagen) according to the manufacturer’s instructions.

    Techniques: Polymerase Chain Reaction, Produced, DNA Sequencing

    Optimization of the DNA extraction procedure. Individual Cq values with median after the duplex qPCR amplification of DNA extracted from T. equi -infected equine blood according to the standard protocol provided with the DNA extraction kit or with modifications that involved preliminary lysis of erythrocytes with RBC Lysis Solution or additional wash of the spin column containing adsorbed DNA with either Buffer AW1 (AW1 Plus) or Buffer AW2 (AW2 Plus). These samples were amplified either with or without BSA in the reaction mixture. Graph was produced using Prism 6

    Journal: Parasites & Vectors

    Article Title: Development and validation of a duplex real-time PCR assay for the diagnosis of equine piroplasmosis

    doi: 10.1186/s13071-018-2751-6

    Figure Lengend Snippet: Optimization of the DNA extraction procedure. Individual Cq values with median after the duplex qPCR amplification of DNA extracted from T. equi -infected equine blood according to the standard protocol provided with the DNA extraction kit or with modifications that involved preliminary lysis of erythrocytes with RBC Lysis Solution or additional wash of the spin column containing adsorbed DNA with either Buffer AW1 (AW1 Plus) or Buffer AW2 (AW2 Plus). These samples were amplified either with or without BSA in the reaction mixture. Graph was produced using Prism 6

    Article Snippet: The extractions were performed either in strict accordance with the manufacturer’s protocol, or with modifications that entailed two consecutive washes of the spin column containing DNA adsorbed to the filter with either Buffer AW1 or Buffer AW2, or the lysis of erythrocytes using RBC Lysis Solution (Qiagen, Germantown, MD, USA) followed by centrifugation and decanting of most of the supernatant containing PCR inhibitors released from the cells prior to the column purification.

    Techniques: DNA Extraction, Real-time Polymerase Chain Reaction, Amplification, Infection, Lysis, Produced

    Optimization of the DNA extraction procedure. Individual Cq values with median after the duplex qPCR amplification of DNA extracted from T. equi -infected equine blood according to the standard protocol provided with the DNA extraction kit or with modifications that involved preliminary lysis of erythrocytes with RBC Lysis Solution or additional wash of the spin column containing adsorbed DNA with either Buffer AW1 (AW1 Plus) or Buffer AW2 (AW2 Plus). These samples were amplified either with or without BSA in the reaction mixture. Graph was produced using Prism 6

    Journal: Parasites & Vectors

    Article Title: Development and validation of a duplex real-time PCR assay for the diagnosis of equine piroplasmosis

    doi: 10.1186/s13071-018-2751-6

    Figure Lengend Snippet: Optimization of the DNA extraction procedure. Individual Cq values with median after the duplex qPCR amplification of DNA extracted from T. equi -infected equine blood according to the standard protocol provided with the DNA extraction kit or with modifications that involved preliminary lysis of erythrocytes with RBC Lysis Solution or additional wash of the spin column containing adsorbed DNA with either Buffer AW1 (AW1 Plus) or Buffer AW2 (AW2 Plus). These samples were amplified either with or without BSA in the reaction mixture. Graph was produced using Prism 6

    Article Snippet: The extractions were performed either in strict accordance with the manufacturer’s protocol, or with modifications that entailed two consecutive washes of the spin column containing DNA adsorbed to the filter with either Buffer AW1 or Buffer AW2, or the lysis of erythrocytes using RBC Lysis Solution (Qiagen, Germantown, MD, USA) followed by centrifugation and decanting of most of the supernatant containing PCR inhibitors released from the cells prior to the column purification.

    Techniques: DNA Extraction, Real-time Polymerase Chain Reaction, Amplification, Infection, Lysis, Produced

    A 2% agarose gel (0.5 μg/mL EtBr) of amplified DNA from RT-PCR of RNA isolated from cells after XPF (left) and XPG (right) knockdown. 1×TBE was used for the gel and running buffer. The gel was run at 100 V for 120 min.

    Journal: Chembiochem : a European journal of chemical biology

    Article Title: Role of endonucleases XPF and XPG in nucleotide excision repair of platinated DNA and cisplatin/oxaliplatin cytotoxicity

    doi: 10.1002/cbic.201000724

    Figure Lengend Snippet: A 2% agarose gel (0.5 μg/mL EtBr) of amplified DNA from RT-PCR of RNA isolated from cells after XPF (left) and XPG (right) knockdown. 1×TBE was used for the gel and running buffer. The gel was run at 100 V for 120 min.

    Article Snippet: Purified RNA was used as a template for one-step RT-PCR (OneStep RT-PCR Kit with Q solution, QIAGEN), in which the reverse transcription reaction and the amplification took place in the same tube.

    Techniques: Agarose Gel Electrophoresis, Amplification, Reverse Transcription Polymerase Chain Reaction, Isolation

    Comparison of AOM and Qiagen extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using HSV-positive CSF. Neat HSV1-PC + was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines . The QDM extracted HSV-1 DNA from the serial dilutions was amplified and detected in AOM tubes ( dashed lines ). HSV-negative CSF was extracted by the AOM method and represented by the light solid line . AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.

    Journal: The Journal of molecular diagnostics : JMD

    Article Title: A Single-Tube Nucleic Acid Extraction, Amplification, and Detection Method Using Aluminum Oxide

    doi: 10.2353/jmoldx.2006.040398

    Figure Lengend Snippet: Comparison of AOM and Qiagen extraction efficiencies. Nucleic acid extraction using the AOM method was compared to the QDM extraction method using HSV-positive CSF. Neat HSV1-PC + was diluted 1:100 and 1:100,000 and extracted using the AOM and QDM methods as described in Materials and Methods. Equal amounts of input nucleic acid were extracted, amplified, and detected by real-time PCR. The AOM extracted, amplified, and detected HSV dilution series is represented by solid lines . The QDM extracted HSV-1 DNA from the serial dilutions was amplified and detected in AOM tubes ( dashed lines ). HSV-negative CSF was extracted by the AOM method and represented by the light solid line . AOM extractions were performed in quadruplicate and the QDM extractions were performed in parallel.

    Article Snippet: The nonproprietary, α-casein lysis buffer is well suited for the extraction of HSV DNA from CSF and yields comparable results to Qiagen AL buffer.

    Techniques: Amplification, Real-time Polymerase Chain Reaction