polyethylenimine Search Results


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  • 99
    Millipore polyethylenimine
    Hemolysis assay on RBCs ( A ) and cytotoxicity of PP-NPs and PPP-NPs on HK-2 cells ( B ). The internalization of PP Cy5 -RH-NPs ( C ) and PPP Cy5 -RH-NPs ( D ) on HK-2 cells was tested by flow cytometry. ( E ) Mean fluorescence intensity curves vs time. PP-NPs, PEG-PCL nanoparticles without loading RH; PPP-NPs, PEG-PCL-PEI nanoparticles without loading RH. Abbreviations: Cy5, cyanine 5; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co <t>-polyethylenimine;</t> RBC, red blood cell; RH, rhein.
    Polyethylenimine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6012 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore polyethylenimine pei
    General scheme of the whole-cell bioluminescence biosensor for detection of oligomerization. Note: Schematic representation explains split-luciferase strategy for monitoring of A53T αS oligomerization and using of this system for screening of GQDs effect on A53T αS oligomerization. Abbreviations: αS, alpha-synuclein; <t>PEI,</t> <t>polyethylenimine;</t> GQDs, graphene quantum dots.
    Polyethylenimine Pei, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore polyethylenimine hydrochloride
    General scheme of the whole-cell bioluminescence biosensor for detection of oligomerization. Note: Schematic representation explains split-luciferase strategy for monitoring of A53T αS oligomerization and using of this system for screening of GQDs effect on A53T αS oligomerization. Abbreviations: αS, alpha-synuclein; <t>PEI,</t> <t>polyethylenimine;</t> GQDs, graphene quantum dots.
    Polyethylenimine Hydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore polyethylenimine reagent
    General scheme of the whole-cell bioluminescence biosensor for detection of oligomerization. Note: Schematic representation explains split-luciferase strategy for monitoring of A53T αS oligomerization and using of this system for screening of GQDs effect on A53T αS oligomerization. Abbreviations: αS, alpha-synuclein; <t>PEI,</t> <t>polyethylenimine;</t> GQDs, graphene quantum dots.
    Polyethylenimine Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Millipore polyethylenimin
    General scheme of the whole-cell bioluminescence biosensor for detection of oligomerization. Note: Schematic representation explains split-luciferase strategy for monitoring of A53T αS oligomerization and using of this system for screening of GQDs effect on A53T αS oligomerization. Abbreviations: αS, alpha-synuclein; <t>PEI,</t> <t>polyethylenimine;</t> GQDs, graphene quantum dots.
    Polyethylenimin, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Mirus Bio polyethylenimine
    General scheme of the whole-cell bioluminescence biosensor for detection of oligomerization. Note: Schematic representation explains split-luciferase strategy for monitoring of A53T αS oligomerization and using of this system for screening of GQDs effect on A53T αS oligomerization. Abbreviations: αS, alpha-synuclein; <t>PEI,</t> <t>polyethylenimine;</t> GQDs, graphene quantum dots.
    Polyethylenimine, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Poly Scientific Inc polyethylenimine
    General scheme of the whole-cell bioluminescence biosensor for detection of oligomerization. Note: Schematic representation explains split-luciferase strategy for monitoring of A53T αS oligomerization and using of this system for screening of GQDs effect on A53T αS oligomerization. Abbreviations: αS, alpha-synuclein; <t>PEI,</t> <t>polyethylenimine;</t> GQDs, graphene quantum dots.
    Polyethylenimine, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 93/100, based on 801 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    PolyScience polyethyleneimine
    as an example) and on the thermally induced functionalization with oligomeric <t>polyethyleneimine</t> (PEI). For the cartoon on carbon dot, it is generally a small carbon nanoparticle core with attached and strongly adsorbed surface passivation molecules (a configuration similar to a soft corona).
    Polyethyleneimine, supplied by PolyScience, used in various techniques. Bioz Stars score: 92/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Polysciences inc polyethyleneimine
    Schematic synthesis of brevinin 2R (BR-2R)-linked <t>polyethylenimine</t> (PEI) by EDC/NHS conjugation method
    Polyethyleneimine, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 94/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher polyethyleneimine
    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, <t>polyethyleneimine-block-polylactic</t> acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Polyethyleneimine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 332 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    AstraZeneca polyethylenimine
    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, <t>polyethyleneimine-block-polylactic</t> acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Polyethylenimine, supplied by AstraZeneca, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    GE Healthcare polyethylenimine
    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, <t>polyethyleneimine-block-polylactic</t> acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Polyethylenimine, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Kaneka Corp polyethylenimine
    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, <t>polyethyleneimine-block-polylactic</t> acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Polyethylenimine, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Polyplus Transfection polyethyleneimine
    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, <t>polyethyleneimine-block-polylactic</t> acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Polyethyleneimine, supplied by Polyplus Transfection, used in various techniques. Bioz Stars score: 92/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor polyethylenimine
    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, <t>polyethyleneimine-block-polylactic</t> acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Polyethylenimine, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Hemolysis assay on RBCs ( A ) and cytotoxicity of PP-NPs and PPP-NPs on HK-2 cells ( B ). The internalization of PP Cy5 -RH-NPs ( C ) and PPP Cy5 -RH-NPs ( D ) on HK-2 cells was tested by flow cytometry. ( E ) Mean fluorescence intensity curves vs time. PP-NPs, PEG-PCL nanoparticles without loading RH; PPP-NPs, PEG-PCL-PEI nanoparticles without loading RH. Abbreviations: Cy5, cyanine 5; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RBC, red blood cell; RH, rhein.

    Journal: International Journal of Nanomedicine

    Article Title: Kidney-targeted drug delivery via rhein-loaded polyethyleneglycol-co-polycaprolactone-co-polyethylenimine nanoparticles for diabetic nephropathy therapy

    doi: 10.2147/IJN.S166445

    Figure Lengend Snippet: Hemolysis assay on RBCs ( A ) and cytotoxicity of PP-NPs and PPP-NPs on HK-2 cells ( B ). The internalization of PP Cy5 -RH-NPs ( C ) and PPP Cy5 -RH-NPs ( D ) on HK-2 cells was tested by flow cytometry. ( E ) Mean fluorescence intensity curves vs time. PP-NPs, PEG-PCL nanoparticles without loading RH; PPP-NPs, PEG-PCL-PEI nanoparticles without loading RH. Abbreviations: Cy5, cyanine 5; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RBC, red blood cell; RH, rhein.

    Article Snippet: Polyethylenimine (PEI; molecular weight 1.8 kD) and 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA).

    Techniques: Hemolysis Assay, Flow Cytometry, Cytometry, Fluorescence

    GPC spectra of the polymers ( A ) and the fluorescent scanning spectra of Cy5-labeled polymers ( B ). Fluorescence spectra of pyrene in water-containing series of concentrations of PEG-PCL ( C ) and PEG-PCL-PEI ( D ). The CMC values of PEG-PCL ( E ) and PEG-PCL-PEI ( F ) were calculated from intersection between the fitting curves of I 338 /I 333 vs concentrations and the fitting curves of fluorescence intensity vs concentrations. Abbreviations: CMC, critical micelle concentration; Cy5, cyanine 5; dRI, differential refractive index; GPC, gel permeation chromatography; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RFU, relative fluorescence unit.

    Journal: International Journal of Nanomedicine

    Article Title: Kidney-targeted drug delivery via rhein-loaded polyethyleneglycol-co-polycaprolactone-co-polyethylenimine nanoparticles for diabetic nephropathy therapy

    doi: 10.2147/IJN.S166445

    Figure Lengend Snippet: GPC spectra of the polymers ( A ) and the fluorescent scanning spectra of Cy5-labeled polymers ( B ). Fluorescence spectra of pyrene in water-containing series of concentrations of PEG-PCL ( C ) and PEG-PCL-PEI ( D ). The CMC values of PEG-PCL ( E ) and PEG-PCL-PEI ( F ) were calculated from intersection between the fitting curves of I 338 /I 333 vs concentrations and the fitting curves of fluorescence intensity vs concentrations. Abbreviations: CMC, critical micelle concentration; Cy5, cyanine 5; dRI, differential refractive index; GPC, gel permeation chromatography; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; RFU, relative fluorescence unit.

    Article Snippet: Polyethylenimine (PEI; molecular weight 1.8 kD) and 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA).

    Techniques: Gel Permeation Chromatography, Labeling, Fluorescence, Concentration Assay, GPC Assay

    The synthesis process of the PEG-PCL-PEI polymer ( A ) and the 1 H-NMR spectra of the polymers: ( B ) PEG-PCL, ( C ) PEG-PCL-COCHCH 2 , ( D ) PEI, ( E ) PEG-PCL-PEI. Note: Dotted circle represented as enlarged position. Abbreviations: DMF, N , N -dimethylformamide; NMR, nuclear magnetic resonance; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; PEI, polyethylenimine.

    Journal: International Journal of Nanomedicine

    Article Title: Kidney-targeted drug delivery via rhein-loaded polyethyleneglycol-co-polycaprolactone-co-polyethylenimine nanoparticles for diabetic nephropathy therapy

    doi: 10.2147/IJN.S166445

    Figure Lengend Snippet: The synthesis process of the PEG-PCL-PEI polymer ( A ) and the 1 H-NMR spectra of the polymers: ( B ) PEG-PCL, ( C ) PEG-PCL-COCHCH 2 , ( D ) PEI, ( E ) PEG-PCL-PEI. Note: Dotted circle represented as enlarged position. Abbreviations: DMF, N , N -dimethylformamide; NMR, nuclear magnetic resonance; PEG-PCL, polyethyleneglycol- co -polycaprolactone; PEG-PCL-PEI, polyethyleneglycol- co -polycaprolactone- co -polyethylenimine; PEI, polyethylenimine.

    Article Snippet: Polyethylenimine (PEI; molecular weight 1.8 kD) and 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA).

    Techniques: Nuclear Magnetic Resonance

    General scheme of the whole-cell bioluminescence biosensor for detection of oligomerization. Note: Schematic representation explains split-luciferase strategy for monitoring of A53T αS oligomerization and using of this system for screening of GQDs effect on A53T αS oligomerization. Abbreviations: αS, alpha-synuclein; PEI, polyethylenimine; GQDs, graphene quantum dots.

    Journal: International Journal of Nanomedicine

    Article Title: Investigation of the effects of carbon-based nanomaterials on A53T alpha-synuclein aggregation using a whole-cell recombinant biosensor

    doi: 10.2147/IJN.S144764

    Figure Lengend Snippet: General scheme of the whole-cell bioluminescence biosensor for detection of oligomerization. Note: Schematic representation explains split-luciferase strategy for monitoring of A53T αS oligomerization and using of this system for screening of GQDs effect on A53T αS oligomerization. Abbreviations: αS, alpha-synuclein; PEI, polyethylenimine; GQDs, graphene quantum dots.

    Article Snippet: Then, the plasmid mixtures were prepared at an N:P ratio of 12 with branched 25 kDa polyethylenimine (PEI) (Sigma-Aldrich, St Louis, MO, USA), and transfection mixture was applied dropwise to each well and incubated with 5% CO2 for 4 hours at 37°C.

    Techniques: Luciferase

    as an example) and on the thermally induced functionalization with oligomeric polyethyleneimine (PEI). For the cartoon on carbon dot, it is generally a small carbon nanoparticle core with attached and strongly adsorbed surface passivation molecules (a configuration similar to a soft corona).

    Journal: Journal of materials chemistry. C, Materials for optical and electronic devices

    Article Title: Photoexcited State Properties of Carbon Dots from Thermally Induced Functionalization of Carbon Nanoparticles

    doi: 10.1039/C6TC03666J

    Figure Lengend Snippet: as an example) and on the thermally induced functionalization with oligomeric polyethyleneimine (PEI). For the cartoon on carbon dot, it is generally a small carbon nanoparticle core with attached and strongly adsorbed surface passivation molecules (a configuration similar to a soft corona).

    Article Snippet: The carbon nanopowder sample was purchased from US Research Nanomaterials, Inc., polyethyleneimine (PEI, branched, average molecular weight ~1,200) from Polyscience, Inc., and silicon carbide (120 Grit) from Panadyne Abrasives.

    Techniques:

    Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by EDC/NHS conjugation method

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Brevinin-2R-linked polyethylenimine as a promising hybrid nano-gene-delivery vector

    doi: 10.22038/ijbms.2019.37125.8842

    Figure Lengend Snippet: Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by EDC/NHS conjugation method

    Article Snippet: Branched polyethyleneimine (b-PEI 10 kDa) has been procured from Polysciences Inc. (Warrington, PA, USA).

    Techniques: Conjugation Assay

    Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by 6-bromohexanoic acid linker

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Brevinin-2R-linked polyethylenimine as a promising hybrid nano-gene-delivery vector

    doi: 10.22038/ijbms.2019.37125.8842

    Figure Lengend Snippet: Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by 6-bromohexanoic acid linker

    Article Snippet: Branched polyethyleneimine (b-PEI 10 kDa) has been procured from Polysciences Inc. (Warrington, PA, USA).

    Techniques:

    Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by SPDP conjugation method

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Brevinin-2R-linked polyethylenimine as a promising hybrid nano-gene-delivery vector

    doi: 10.22038/ijbms.2019.37125.8842

    Figure Lengend Snippet: Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by SPDP conjugation method

    Article Snippet: Branched polyethyleneimine (b-PEI 10 kDa) has been procured from Polysciences Inc. (Warrington, PA, USA).

    Techniques: Conjugation Assay

    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Journal: International Journal of Nanomedicine

    Article Title: Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes

    doi: 10.2147/IJN.S161426

    Figure Lengend Snippet: Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Article Snippet: Branched polyethyleneimine (MW=1.8 kDa, bPEI1.8k ) was purchased from Alfa Aesar (Ward Hill, MA, USA).

    Techniques: Fluorescence, Mouse Assay, Injection, Proximity Ligation Assay, Blocking Assay

    ( A ) PTX/siRNA-loaded layer-by-layer nanoparticle delivery system. ( B ) Schematic of the intracellular therapeutic mechanism of the PTX/siRNA-loaded layer-by-layer nanoparticles. Abbreviations: PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt); EPR, enhanced permeation and retention.

    Journal: International Journal of Nanomedicine

    Article Title: Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes

    doi: 10.2147/IJN.S161426

    Figure Lengend Snippet: ( A ) PTX/siRNA-loaded layer-by-layer nanoparticle delivery system. ( B ) Schematic of the intracellular therapeutic mechanism of the PTX/siRNA-loaded layer-by-layer nanoparticles. Abbreviations: PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt); EPR, enhanced permeation and retention.

    Article Snippet: Branched polyethyleneimine (MW=1.8 kDa, bPEI1.8k ) was purchased from Alfa Aesar (Ward Hill, MA, USA).

    Techniques: Proximity Ligation Assay, Blocking Assay, Electron Paramagnetic Resonance

    Cellular uptake of siRNA or PTX in different formulations. A549 cells were analyzed after 4 h of incubation at the final concentrations of different NPs (Oregon Green PTX content 200 ng/mL, Cy3 siRNA 50 nM, N/P=30, C/N=1/5). ( A ) Quantitative analyses of siRNA and PTX uptake by flow cytometry. Fluorescence signals of Cy3 and Oregon Green PTX are presented by four-quadrant diagram and histogram (400×). (a) Mock; (b) siRNA; (c) PEI-PLA/siRNA/PEG-PAsp; (d) PTX; (e) PEI-PLA/PTX/PEG-PAsp; (f) PEI-PLA/siRNA/PTX; (g) PEI-PLA/siRNA/PTX/PEG-PAsp. ( B ) Confocal laser scanning microscope images of cells treated with different formulations of NPs: (a) siRNA; (b) PEI-PLA/siRNA/PEG-PAsp; (c) PTX; (d) PEI-PLA/PTX/PEG-PAsp; (e) PEI-PLA/siRNA/PTX/PEG-PAsp. For each column, from left to right: nuclei were stained by DAPI (blue); Oregon Green PTX fluorescence in cells (green); Cy3 signal in cells (red); merged with nucleus, Cy3-siRNA, and Oregon Green PTX. The yellow stains (arrows) in the cytoplasm indicates that the NPs successfully enters into the cell nucleus, and could easily escape from the endosomes via the proton sponge effect of PEI. Abbreviations: PTX, paclitaxel; NPs, nanoparticles; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Journal: International Journal of Nanomedicine

    Article Title: Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes

    doi: 10.2147/IJN.S161426

    Figure Lengend Snippet: Cellular uptake of siRNA or PTX in different formulations. A549 cells were analyzed after 4 h of incubation at the final concentrations of different NPs (Oregon Green PTX content 200 ng/mL, Cy3 siRNA 50 nM, N/P=30, C/N=1/5). ( A ) Quantitative analyses of siRNA and PTX uptake by flow cytometry. Fluorescence signals of Cy3 and Oregon Green PTX are presented by four-quadrant diagram and histogram (400×). (a) Mock; (b) siRNA; (c) PEI-PLA/siRNA/PEG-PAsp; (d) PTX; (e) PEI-PLA/PTX/PEG-PAsp; (f) PEI-PLA/siRNA/PTX; (g) PEI-PLA/siRNA/PTX/PEG-PAsp. ( B ) Confocal laser scanning microscope images of cells treated with different formulations of NPs: (a) siRNA; (b) PEI-PLA/siRNA/PEG-PAsp; (c) PTX; (d) PEI-PLA/PTX/PEG-PAsp; (e) PEI-PLA/siRNA/PTX/PEG-PAsp. For each column, from left to right: nuclei were stained by DAPI (blue); Oregon Green PTX fluorescence in cells (green); Cy3 signal in cells (red); merged with nucleus, Cy3-siRNA, and Oregon Green PTX. The yellow stains (arrows) in the cytoplasm indicates that the NPs successfully enters into the cell nucleus, and could easily escape from the endosomes via the proton sponge effect of PEI. Abbreviations: PTX, paclitaxel; NPs, nanoparticles; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Article Snippet: Branched polyethyleneimine (MW=1.8 kDa, bPEI1.8k ) was purchased from Alfa Aesar (Ward Hill, MA, USA).

    Techniques: Incubation, Flow Cytometry, Cytometry, Fluorescence, Proximity Ligation Assay, Laser-Scanning Microscopy, Staining, Blocking Assay

    Characterization of the NPs. ( A ) Particle size (intensity diameter) and zeta potential of PEI-PLA/PTX/siRNA/PEG-PAsp NPs. ( B ) Transmission electron microscopy image of PEI-PLA/PTX/siRNA/PEG-PAsp NPs. The scale bar represents 100 nm. Abbreviations: NPs, nanoparticles; PEI-PLA, polyethyleneimine-block-polylactic acid; PTX, paclitaxel; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Journal: International Journal of Nanomedicine

    Article Title: Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes

    doi: 10.2147/IJN.S161426

    Figure Lengend Snippet: Characterization of the NPs. ( A ) Particle size (intensity diameter) and zeta potential of PEI-PLA/PTX/siRNA/PEG-PAsp NPs. ( B ) Transmission electron microscopy image of PEI-PLA/PTX/siRNA/PEG-PAsp NPs. The scale bar represents 100 nm. Abbreviations: NPs, nanoparticles; PEI-PLA, polyethyleneimine-block-polylactic acid; PTX, paclitaxel; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Article Snippet: Branched polyethyleneimine (MW=1.8 kDa, bPEI1.8k ) was purchased from Alfa Aesar (Ward Hill, MA, USA).

    Techniques: Proximity Ligation Assay, Transmission Assay, Electron Microscopy, Blocking Assay

    ( A ) Synthesis of the PEI-PLA. The PLA-COOH was conjugated to PEI through amide formation in the presence of EDC and NHS, obtaining the cationic amphiphilic copolymer of PEI-PLA. ( B ) 1 H-NMR spectra of PEI and PEI-PLA in D 2 O. The peak of PEI appears at around 2.6 ppm. In PEI-PLA, a new broad peak appears at 2.3–3.4 ppm, which is attributed to the protons of methylene (–CH 2 CH 2 –) in PEI. The signals at δ =1.20 ppm (a) and δ =4.08 ppm (b) correspond to −CH 3 and (–CH) proton in the PLA block of PEI-PLA, respectively. Abbreviations: PEI-PLA, polyethyleneimine-block-polylactic acid; EDC, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride; NHS, N -hydroxysuccinimide; NMR, nuclear magnetic resonance.

    Journal: International Journal of Nanomedicine

    Article Title: Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes

    doi: 10.2147/IJN.S161426

    Figure Lengend Snippet: ( A ) Synthesis of the PEI-PLA. The PLA-COOH was conjugated to PEI through amide formation in the presence of EDC and NHS, obtaining the cationic amphiphilic copolymer of PEI-PLA. ( B ) 1 H-NMR spectra of PEI and PEI-PLA in D 2 O. The peak of PEI appears at around 2.6 ppm. In PEI-PLA, a new broad peak appears at 2.3–3.4 ppm, which is attributed to the protons of methylene (–CH 2 CH 2 –) in PEI. The signals at δ =1.20 ppm (a) and δ =4.08 ppm (b) correspond to −CH 3 and (–CH) proton in the PLA block of PEI-PLA, respectively. Abbreviations: PEI-PLA, polyethyleneimine-block-polylactic acid; EDC, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride; NHS, N -hydroxysuccinimide; NMR, nuclear magnetic resonance.

    Article Snippet: Branched polyethyleneimine (MW=1.8 kDa, bPEI1.8k ) was purchased from Alfa Aesar (Ward Hill, MA, USA).

    Techniques: Proximity Ligation Assay, Nuclear Magnetic Resonance, Blocking Assay

    ( A ) Nuclear morphology, ( B ) cell apoptosis, and ( C ) cell cycle change in A549 cells 48 h after treatment with various formulations. Each row, from top to bottom, presents Hoechst staining of cell nuclei, quantitative detection of apoptotic cells, and cell cycle study. For Hoechst staining, cells were stained with Hoechst 33258 reagent at a concentration of 5 µg/mL for 10 min. For the cell apoptotic assay, cells were stained with Annexin V-FITC/PI for 15 min. For the cell cycle assay, cells were stained with PI (50 µg/mL) for 1 h. Chromatin condensation, nuclear fragmentation, and chromosome abnormalities were found in the PEI-PLA/siRNA/PEG-PAsp, PEI-PLA/PTX/siRNAN.C/PEG-PAsp and PEI-PLA/PTX/siRNA/PEG-PAsp groups (arrows). Abbreviations: FITC, fluorescein isothiocyanate; PI, propidium iodide; PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Journal: International Journal of Nanomedicine

    Article Title: Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes

    doi: 10.2147/IJN.S161426

    Figure Lengend Snippet: ( A ) Nuclear morphology, ( B ) cell apoptosis, and ( C ) cell cycle change in A549 cells 48 h after treatment with various formulations. Each row, from top to bottom, presents Hoechst staining of cell nuclei, quantitative detection of apoptotic cells, and cell cycle study. For Hoechst staining, cells were stained with Hoechst 33258 reagent at a concentration of 5 µg/mL for 10 min. For the cell apoptotic assay, cells were stained with Annexin V-FITC/PI for 15 min. For the cell cycle assay, cells were stained with PI (50 µg/mL) for 1 h. Chromatin condensation, nuclear fragmentation, and chromosome abnormalities were found in the PEI-PLA/siRNA/PEG-PAsp, PEI-PLA/PTX/siRNAN.C/PEG-PAsp and PEI-PLA/PTX/siRNA/PEG-PAsp groups (arrows). Abbreviations: FITC, fluorescein isothiocyanate; PI, propidium iodide; PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Article Snippet: Branched polyethyleneimine (MW=1.8 kDa, bPEI1.8k ) was purchased from Alfa Aesar (Ward Hill, MA, USA).

    Techniques: Staining, Concentration Assay, Cell Cycle Assay, Proximity Ligation Assay, Blocking Assay