Journal: International Journal of Nanomedicine
Article Title: Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes
Figure Lengend Snippet: ( A ) Nuclear morphology, ( B ) cell apoptosis, and ( C ) cell cycle change in A549 cells 48 h after treatment with various formulations. Each row, from top to bottom, presents Hoechst staining of cell nuclei, quantitative detection of apoptotic cells, and cell cycle study. For Hoechst staining, cells were stained with Hoechst 33258 reagent at a concentration of 5 µg/mL for 10 min. For the cell apoptotic assay, cells were stained with Annexin V-FITC/PI for 15 min. For the cell cycle assay, cells were stained with PI (50 µg/mL) for 1 h. Chromatin condensation, nuclear fragmentation, and chromosome abnormalities were found in the PEI-PLA/siRNA/PEG-PAsp, PEI-PLA/PTX/siRNAN.C/PEG-PAsp and PEI-PLA/PTX/siRNA/PEG-PAsp groups (arrows). Abbreviations: FITC, fluorescein isothiocyanate; PI, propidium iodide; PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
Article Snippet: Branched polyethyleneimine (MW=1.8 kDa, bPEI1.8k ) was purchased from Alfa Aesar (Ward Hill, MA, USA).
Techniques: Staining, Concentration Assay, Cell Cycle Assay, Proximity Ligation Assay, Blocking Assay