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  • 99
    Millipore polyethylenimine
    Polyethylenimine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5995 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Polysciences inc polyethyleneimine
    Schematic synthesis of brevinin 2R (BR-2R)-linked <t>polyethylenimine</t> (PEI) by EDC/NHS conjugation method
    Polyethyleneimine, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 94/100, based on 2881 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    PolyScience polyethyleneimine
    as an example) and on the thermally induced functionalization with oligomeric <t>polyethyleneimine</t> (PEI). For the cartoon on carbon dot, it is generally a small carbon nanoparticle core with attached and strongly adsorbed surface passivation molecules (a configuration similar to a soft corona).
    Polyethyleneimine, supplied by PolyScience, used in various techniques. Bioz Stars score: 92/100, based on 258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Polysciences inc polyethylenimine pei
    Recombinant TRIM25 co-purifies with nucleic acid. ( a ) Affinity purification scheme for Strep/FLAG-tagged TRIM25 expressed in insect cells. ( b ) Coomassie-stained SDS-PAGE gels of TRIM25 fractions after initial affinity chromatography step. ( c ) UV absorbance spectra of pooled protein fractions. Vertical dashed lines indicate the peak wavelength. ( d ) Visualization by agarose gel electrophoresis and SYBR-green staining of the co-purifying nucleic acid (−PEI) and its removal by <t>polyethyleneimine</t> treatment <t>(+PEI).</t>
    Polyethylenimine Pei, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 94/100, based on 1789 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    PolyScience polyethylenimine pei
    Recombinant TRIM25 co-purifies with nucleic acid. ( a ) Affinity purification scheme for Strep/FLAG-tagged TRIM25 expressed in insect cells. ( b ) Coomassie-stained SDS-PAGE gels of TRIM25 fractions after initial affinity chromatography step. ( c ) UV absorbance spectra of pooled protein fractions. Vertical dashed lines indicate the peak wavelength. ( d ) Visualization by agarose gel electrophoresis and SYBR-green staining of the co-purifying nucleic acid (−PEI) and its removal by <t>polyethyleneimine</t> treatment <t>(+PEI).</t>
    Polyethylenimine Pei, supplied by PolyScience, used in various techniques. Bioz Stars score: 92/100, based on 197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore polyethyleneimine pei
    Recombinant TRIM25 co-purifies with nucleic acid. ( a ) Affinity purification scheme for Strep/FLAG-tagged TRIM25 expressed in insect cells. ( b ) Coomassie-stained SDS-PAGE gels of TRIM25 fractions after initial affinity chromatography step. ( c ) UV absorbance spectra of pooled protein fractions. Vertical dashed lines indicate the peak wavelength. ( d ) Visualization by agarose gel electrophoresis and SYBR-green staining of the co-purifying nucleic acid (−PEI) and its removal by <t>polyethyleneimine</t> treatment <t>(+PEI).</t>
    Polyethyleneimine Pei, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 449 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Poly Scientific Inc polyethyleneimine
    Recombinant TRIM25 co-purifies with nucleic acid. ( a ) Affinity purification scheme for Strep/FLAG-tagged TRIM25 expressed in insect cells. ( b ) Coomassie-stained SDS-PAGE gels of TRIM25 fractions after initial affinity chromatography step. ( c ) UV absorbance spectra of pooled protein fractions. Vertical dashed lines indicate the peak wavelength. ( d ) Visualization by agarose gel electrophoresis and SYBR-green staining of the co-purifying nucleic acid (−PEI) and its removal by <t>polyethyleneimine</t> treatment <t>(+PEI).</t>
    Polyethyleneimine, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 93/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher polyethyleneimine
    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, <t>polyethyleneimine-block-polylactic</t> acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Polyethyleneimine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 332 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore polyethylenimine branched
    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, <t>polyethyleneimine-block-polylactic</t> acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Polyethylenimine Branched, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Poly Scientific Inc polyethyleneimine pei
    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, <t>polyethyleneimine-block-polylactic</t> acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).
    Polyethyleneimine Pei, supplied by Poly Scientific Inc, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Merck & Co polyethyleneimine cellulose plates
    In vitro GAP activity of Git2-short toward ARF1. ARF1 (0.5 μg) preloaded with [α- 32 P]GTP were incubated for 5 min at 30°C with buffer alone (lane 1), with 2.5 μg of Git2-short (lane 2), or the CA mutant (lane 3). The nucleotides were then extracted and separated on a <t>polyethyleneimine-cellulose</t> plate and subjected to autoradiography. Positions of GTP and GDP were identified using authentic nucleotides.
    Polyethyleneimine Cellulose Plates, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Polysciences inc 25 kda linear polyethylenimines
    In vitro GAP activity of Git2-short toward ARF1. ARF1 (0.5 μg) preloaded with [α- 32 P]GTP were incubated for 5 min at 30°C with buffer alone (lane 1), with 2.5 μg of Git2-short (lane 2), or the CA mutant (lane 3). The nucleotides were then extracted and separated on a <t>polyethyleneimine-cellulose</t> plate and subjected to autoradiography. Positions of GTP and GDP were identified using authentic nucleotides.
    25 Kda Linear Polyethylenimines, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Polyplus Transfection polyethyleneimine
    In vitro GAP activity of Git2-short toward ARF1. ARF1 (0.5 μg) preloaded with [α- 32 P]GTP were incubated for 5 min at 30°C with buffer alone (lane 1), with 2.5 μg of Git2-short (lane 2), or the CA mutant (lane 3). The nucleotides were then extracted and separated on a <t>polyethyleneimine-cellulose</t> plate and subjected to autoradiography. Positions of GTP and GDP were identified using authentic nucleotides.
    Polyethyleneimine, supplied by Polyplus Transfection, used in various techniques. Bioz Stars score: 92/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore ion exchange
    In vitro GAP activity of Git2-short toward ARF1. ARF1 (0.5 μg) preloaded with [α- 32 P]GTP were incubated for 5 min at 30°C with buffer alone (lane 1), with 2.5 μg of Git2-short (lane 2), or the CA mutant (lane 3). The nucleotides were then extracted and separated on a <t>polyethyleneimine-cellulose</t> plate and subjected to autoradiography. Positions of GTP and GDP were identified using authentic nucleotides.
    Ion Exchange, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Polysciences inc polyethylenimine pei transfection reagent
    In vitro GAP activity of Git2-short toward ARF1. ARF1 (0.5 μg) preloaded with [α- 32 P]GTP were incubated for 5 min at 30°C with buffer alone (lane 1), with 2.5 μg of Git2-short (lane 2), or the CA mutant (lane 3). The nucleotides were then extracted and separated on a <t>polyethyleneimine-cellulose</t> plate and subjected to autoradiography. Positions of GTP and GDP were identified using authentic nucleotides.
    Polyethylenimine Pei Transfection Reagent, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 89/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Polysciences inc polyethylenimine reagent
    In vitro GAP activity of Git2-short toward ARF1. ARF1 (0.5 μg) preloaded with [α- 32 P]GTP were incubated for 5 min at 30°C with buffer alone (lane 1), with 2.5 μg of Git2-short (lane 2), or the CA mutant (lane 3). The nucleotides were then extracted and separated on a <t>polyethyleneimine-cellulose</t> plate and subjected to autoradiography. Positions of GTP and GDP were identified using authentic nucleotides.
    Polyethylenimine Reagent, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 89/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Polyplus Transfection polyethylenimine pei
    In vitro GAP activity of Git2-short toward ARF1. ARF1 (0.5 μg) preloaded with [α- 32 P]GTP were incubated for 5 min at 30°C with buffer alone (lane 1), with 2.5 μg of Git2-short (lane 2), or the CA mutant (lane 3). The nucleotides were then extracted and separated on a <t>polyethyleneimine-cellulose</t> plate and subjected to autoradiography. Positions of GTP and GDP were identified using authentic nucleotides.
    Polyethylenimine Pei, supplied by Polyplus Transfection, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by EDC/NHS conjugation method

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Brevinin-2R-linked polyethylenimine as a promising hybrid nano-gene-delivery vector

    doi: 10.22038/ijbms.2019.37125.8842

    Figure Lengend Snippet: Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by EDC/NHS conjugation method

    Article Snippet: Branched polyethyleneimine (b-PEI 10 kDa) has been procured from Polysciences Inc. (Warrington, PA, USA).

    Techniques: Conjugation Assay

    Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by 6-bromohexanoic acid linker

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Brevinin-2R-linked polyethylenimine as a promising hybrid nano-gene-delivery vector

    doi: 10.22038/ijbms.2019.37125.8842

    Figure Lengend Snippet: Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by 6-bromohexanoic acid linker

    Article Snippet: Branched polyethyleneimine (b-PEI 10 kDa) has been procured from Polysciences Inc. (Warrington, PA, USA).

    Techniques:

    Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by SPDP conjugation method

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Brevinin-2R-linked polyethylenimine as a promising hybrid nano-gene-delivery vector

    doi: 10.22038/ijbms.2019.37125.8842

    Figure Lengend Snippet: Schematic synthesis of brevinin 2R (BR-2R)-linked polyethylenimine (PEI) by SPDP conjugation method

    Article Snippet: Branched polyethyleneimine (b-PEI 10 kDa) has been procured from Polysciences Inc. (Warrington, PA, USA).

    Techniques: Conjugation Assay

    Transfection mediated by linear polyethylenimine (25 KDa) (LPEI)-pDNA (1 µg) nanoparticle complexes and branched polyethylenimine (25 KDa) (BPEI)-pDNA nanoparticle complexes at various N/P ratios in (a) HeLa, (b) HEK, (c) COS7 and (d) HepG2. Luciferase

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo

    doi: 10.1016/j.jconrel.2008.04.014

    Figure Lengend Snippet: Transfection mediated by linear polyethylenimine (25 KDa) (LPEI)-pDNA (1 µg) nanoparticle complexes and branched polyethylenimine (25 KDa) (BPEI)-pDNA nanoparticle complexes at various N/P ratios in (a) HeLa, (b) HEK, (c) COS7 and (d) HepG2. Luciferase

    Article Snippet: Linear polyethylenimine (PEI, MW 25kDa) was obtained from Polysciences Inc. (Warrington, PA).

    Techniques: Transfection, Luciferase

    Chemical structure of (a) linear and (b) branched polyethylenimine

    Journal: Journal of controlled release : official journal of the Controlled Release Society

    Article Title: Characterization of the transgene expression generated by branched and linear polyethylenimine-plasmid DNA nanoparticles in vitro and after intraperitoneal injection in vivo

    doi: 10.1016/j.jconrel.2008.04.014

    Figure Lengend Snippet: Chemical structure of (a) linear and (b) branched polyethylenimine

    Article Snippet: Linear polyethylenimine (PEI, MW 25kDa) was obtained from Polysciences Inc. (Warrington, PA).

    Techniques:

    HeLa cell growth in the presence of polyethylenimine (PEI)-coated magnetic nanoparticles (MNPs) at 300 μg/mL and with MNP/antibody complexes added at 100, 200 and 300 μg/mL. The control did not include these treatments. Cell numbers were normalized by the number of control cells. Cell numbers decreased with increasing complex doses.

    Journal: Nanomaterials

    Article Title: Hyperthermia Using Antibody-Conjugated Magnetic Nanoparticles and Its Enhanced Effect with Cryptotanshinone

    doi: 10.3390/nano4020319

    Figure Lengend Snippet: HeLa cell growth in the presence of polyethylenimine (PEI)-coated magnetic nanoparticles (MNPs) at 300 μg/mL and with MNP/antibody complexes added at 100, 200 and 300 μg/mL. The control did not include these treatments. Cell numbers were normalized by the number of control cells. Cell numbers decreased with increasing complex doses.

    Article Snippet: Materials and Reagents Fe3 O4 nanoparticles (primary diameter, 20–30 nm) were obtained from Nanostructured and Amorphous Materials, Inc. Polyethylenimine max (PEI; mw 40,000) was from Polysciences, Inc.

    Techniques:

    as an example) and on the thermally induced functionalization with oligomeric polyethyleneimine (PEI). For the cartoon on carbon dot, it is generally a small carbon nanoparticle core with attached and strongly adsorbed surface passivation molecules (a configuration similar to a soft corona).

    Journal: Journal of materials chemistry. C, Materials for optical and electronic devices

    Article Title: Photoexcited State Properties of Carbon Dots from Thermally Induced Functionalization of Carbon Nanoparticles

    doi: 10.1039/C6TC03666J

    Figure Lengend Snippet: as an example) and on the thermally induced functionalization with oligomeric polyethyleneimine (PEI). For the cartoon on carbon dot, it is generally a small carbon nanoparticle core with attached and strongly adsorbed surface passivation molecules (a configuration similar to a soft corona).

    Article Snippet: The carbon nanopowder sample was purchased from US Research Nanomaterials, Inc., polyethyleneimine (PEI, branched, average molecular weight ~1,200) from Polyscience, Inc., and silicon carbide (120 Grit) from Panadyne Abrasives.

    Techniques:

    Recombinant TRIM25 co-purifies with nucleic acid. ( a ) Affinity purification scheme for Strep/FLAG-tagged TRIM25 expressed in insect cells. ( b ) Coomassie-stained SDS-PAGE gels of TRIM25 fractions after initial affinity chromatography step. ( c ) UV absorbance spectra of pooled protein fractions. Vertical dashed lines indicate the peak wavelength. ( d ) Visualization by agarose gel electrophoresis and SYBR-green staining of the co-purifying nucleic acid (−PEI) and its removal by polyethyleneimine treatment (+PEI).

    Journal: Journal of molecular biology

    Article Title: TRIM25 binds RNA to modulate cellular anti-viral defense

    doi: 10.1016/j.jmb.2018.10.003

    Figure Lengend Snippet: Recombinant TRIM25 co-purifies with nucleic acid. ( a ) Affinity purification scheme for Strep/FLAG-tagged TRIM25 expressed in insect cells. ( b ) Coomassie-stained SDS-PAGE gels of TRIM25 fractions after initial affinity chromatography step. ( c ) UV absorbance spectra of pooled protein fractions. Vertical dashed lines indicate the peak wavelength. ( d ) Visualization by agarose gel electrophoresis and SYBR-green staining of the co-purifying nucleic acid (−PEI) and its removal by polyethyleneimine treatment (+PEI).

    Article Snippet: TRIM25 -KO HEK 293T cells [ ], seeded into 12-well plates (~5 × 105 cells/well), were transfected with 2 μg of pCMV empty vector or the indicated FLAG-tagged TRIM25 constructs using linear polyethylenimine (PEI) (1 mg/mL solution in 20 mM Tris pH 6.8; Polysciences), according to the manufacturer’s instructions.

    Techniques: Recombinant, Affinity Purification, Staining, SDS Page, Affinity Chromatography, Agarose Gel Electrophoresis, SYBR Green Assay

    Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Journal: International Journal of Nanomedicine

    Article Title: Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes

    doi: 10.2147/IJN.S161426

    Figure Lengend Snippet: Fluorescence images of organs and tumors in A549 tumor-bearing mice 24 h after intravenous injection of complex nanoparticles (DIR=50 µg/kg, siRNA Cy5 =2 mg/kg) (n=3). ( A ) Fluorescence image of DIR channel: (a) DIR; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. ( B ) Fluorescence image of Cy5 channel: (a) siRNA Cy5 ; (b) PEI-PLA/DIR/siRNA Cy5 ; (c) PEI-PLA/DIR/siRNA Cy5 /PEG-PAsp. Abbreviations: PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Article Snippet: Branched polyethyleneimine (MW=1.8 kDa, bPEI1.8k ) was purchased from Alfa Aesar (Ward Hill, MA, USA).

    Techniques: Fluorescence, Mouse Assay, Injection, Proximity Ligation Assay, Blocking Assay

    ( A ) PTX/siRNA-loaded layer-by-layer nanoparticle delivery system. ( B ) Schematic of the intracellular therapeutic mechanism of the PTX/siRNA-loaded layer-by-layer nanoparticles. Abbreviations: PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt); EPR, enhanced permeation and retention.

    Journal: International Journal of Nanomedicine

    Article Title: Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes

    doi: 10.2147/IJN.S161426

    Figure Lengend Snippet: ( A ) PTX/siRNA-loaded layer-by-layer nanoparticle delivery system. ( B ) Schematic of the intracellular therapeutic mechanism of the PTX/siRNA-loaded layer-by-layer nanoparticles. Abbreviations: PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt); EPR, enhanced permeation and retention.

    Article Snippet: Branched polyethyleneimine (MW=1.8 kDa, bPEI1.8k ) was purchased from Alfa Aesar (Ward Hill, MA, USA).

    Techniques: Proximity Ligation Assay, Blocking Assay, Electron Paramagnetic Resonance

    Cellular uptake of siRNA or PTX in different formulations. A549 cells were analyzed after 4 h of incubation at the final concentrations of different NPs (Oregon Green PTX content 200 ng/mL, Cy3 siRNA 50 nM, N/P=30, C/N=1/5). ( A ) Quantitative analyses of siRNA and PTX uptake by flow cytometry. Fluorescence signals of Cy3 and Oregon Green PTX are presented by four-quadrant diagram and histogram (400×). (a) Mock; (b) siRNA; (c) PEI-PLA/siRNA/PEG-PAsp; (d) PTX; (e) PEI-PLA/PTX/PEG-PAsp; (f) PEI-PLA/siRNA/PTX; (g) PEI-PLA/siRNA/PTX/PEG-PAsp. ( B ) Confocal laser scanning microscope images of cells treated with different formulations of NPs: (a) siRNA; (b) PEI-PLA/siRNA/PEG-PAsp; (c) PTX; (d) PEI-PLA/PTX/PEG-PAsp; (e) PEI-PLA/siRNA/PTX/PEG-PAsp. For each column, from left to right: nuclei were stained by DAPI (blue); Oregon Green PTX fluorescence in cells (green); Cy3 signal in cells (red); merged with nucleus, Cy3-siRNA, and Oregon Green PTX. The yellow stains (arrows) in the cytoplasm indicates that the NPs successfully enters into the cell nucleus, and could easily escape from the endosomes via the proton sponge effect of PEI. Abbreviations: PTX, paclitaxel; NPs, nanoparticles; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Journal: International Journal of Nanomedicine

    Article Title: Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes

    doi: 10.2147/IJN.S161426

    Figure Lengend Snippet: Cellular uptake of siRNA or PTX in different formulations. A549 cells were analyzed after 4 h of incubation at the final concentrations of different NPs (Oregon Green PTX content 200 ng/mL, Cy3 siRNA 50 nM, N/P=30, C/N=1/5). ( A ) Quantitative analyses of siRNA and PTX uptake by flow cytometry. Fluorescence signals of Cy3 and Oregon Green PTX are presented by four-quadrant diagram and histogram (400×). (a) Mock; (b) siRNA; (c) PEI-PLA/siRNA/PEG-PAsp; (d) PTX; (e) PEI-PLA/PTX/PEG-PAsp; (f) PEI-PLA/siRNA/PTX; (g) PEI-PLA/siRNA/PTX/PEG-PAsp. ( B ) Confocal laser scanning microscope images of cells treated with different formulations of NPs: (a) siRNA; (b) PEI-PLA/siRNA/PEG-PAsp; (c) PTX; (d) PEI-PLA/PTX/PEG-PAsp; (e) PEI-PLA/siRNA/PTX/PEG-PAsp. For each column, from left to right: nuclei were stained by DAPI (blue); Oregon Green PTX fluorescence in cells (green); Cy3 signal in cells (red); merged with nucleus, Cy3-siRNA, and Oregon Green PTX. The yellow stains (arrows) in the cytoplasm indicates that the NPs successfully enters into the cell nucleus, and could easily escape from the endosomes via the proton sponge effect of PEI. Abbreviations: PTX, paclitaxel; NPs, nanoparticles; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Article Snippet: Branched polyethyleneimine (MW=1.8 kDa, bPEI1.8k ) was purchased from Alfa Aesar (Ward Hill, MA, USA).

    Techniques: Incubation, Flow Cytometry, Cytometry, Fluorescence, Proximity Ligation Assay, Laser-Scanning Microscopy, Staining, Blocking Assay

    ( A ) Nuclear morphology, ( B ) cell apoptosis, and ( C ) cell cycle change in A549 cells 48 h after treatment with various formulations. Each row, from top to bottom, presents Hoechst staining of cell nuclei, quantitative detection of apoptotic cells, and cell cycle study. For Hoechst staining, cells were stained with Hoechst 33258 reagent at a concentration of 5 µg/mL for 10 min. For the cell apoptotic assay, cells were stained with Annexin V-FITC/PI for 15 min. For the cell cycle assay, cells were stained with PI (50 µg/mL) for 1 h. Chromatin condensation, nuclear fragmentation, and chromosome abnormalities were found in the PEI-PLA/siRNA/PEG-PAsp, PEI-PLA/PTX/siRNAN.C/PEG-PAsp and PEI-PLA/PTX/siRNA/PEG-PAsp groups (arrows). Abbreviations: FITC, fluorescein isothiocyanate; PI, propidium iodide; PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Journal: International Journal of Nanomedicine

    Article Title: Smart polymeric nanoparticles with pH-responsive and PEG-detachable properties for co-delivering paclitaxel and survivin siRNA to enhance antitumor outcomes

    doi: 10.2147/IJN.S161426

    Figure Lengend Snippet: ( A ) Nuclear morphology, ( B ) cell apoptosis, and ( C ) cell cycle change in A549 cells 48 h after treatment with various formulations. Each row, from top to bottom, presents Hoechst staining of cell nuclei, quantitative detection of apoptotic cells, and cell cycle study. For Hoechst staining, cells were stained with Hoechst 33258 reagent at a concentration of 5 µg/mL for 10 min. For the cell apoptotic assay, cells were stained with Annexin V-FITC/PI for 15 min. For the cell cycle assay, cells were stained with PI (50 µg/mL) for 1 h. Chromatin condensation, nuclear fragmentation, and chromosome abnormalities were found in the PEI-PLA/siRNA/PEG-PAsp, PEI-PLA/PTX/siRNAN.C/PEG-PAsp and PEI-PLA/PTX/siRNA/PEG-PAsp groups (arrows). Abbreviations: FITC, fluorescein isothiocyanate; PI, propidium iodide; PTX, paclitaxel; PEI-PLA, polyethyleneimine-block-polylactic acid; PEG-PAsp, poly(ethylene glycol)-block-poly(L-aspartic acid sodium salt).

    Article Snippet: Branched polyethyleneimine (MW=1.8 kDa, bPEI1.8k ) was purchased from Alfa Aesar (Ward Hill, MA, USA).

    Techniques: Staining, Concentration Assay, Cell Cycle Assay, Proximity Ligation Assay, Blocking Assay

    In vitro GAP activity of Git2-short toward ARF1. ARF1 (0.5 μg) preloaded with [α- 32 P]GTP were incubated for 5 min at 30°C with buffer alone (lane 1), with 2.5 μg of Git2-short (lane 2), or the CA mutant (lane 3). The nucleotides were then extracted and separated on a polyethyleneimine-cellulose plate and subjected to autoradiography. Positions of GTP and GDP were identified using authentic nucleotides.

    Journal: Molecular Biology of the Cell

    Article Title: An ADP-Ribosylation Factor GTPase-activating Protein Git2-short/KIAA0148 Is Involved in Subcellular Localization of Paxillin and Actin Cytoskeletal Organization

    doi:

    Figure Lengend Snippet: In vitro GAP activity of Git2-short toward ARF1. ARF1 (0.5 μg) preloaded with [α- 32 P]GTP were incubated for 5 min at 30°C with buffer alone (lane 1), with 2.5 μg of Git2-short (lane 2), or the CA mutant (lane 3). The nucleotides were then extracted and separated on a polyethyleneimine-cellulose plate and subjected to autoradiography. Positions of GTP and GDP were identified using authentic nucleotides.

    Article Snippet: Bound nucleotides were eluted in 2 M formic acid and were separated by chromatography on polyethyleneimine-cellulose plates (Merck, Rahway, NJ) in 1 M formic acid/1 M LiCl.

    Techniques: In Vitro, Activity Assay, Incubation, Mutagenesis, Autoradiography